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Društvo za jezičke resurse i tehnologije 13

[ "Bugarski, Diana" ]

[ "Korać, Aleksandra", "Santibañez, Juan" ]

[ "2016-01-05T11:46:17", "2020-07-03T08:08:30" ]

[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=64", "https://nardus.mpn.gov.rs/handle/123456789/2060", "https://fedorabg.bg.ac.rs/fedora/get/o:3419/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024511922" ]

[ "Interleukin (IL) 17 familija citokina se smatra najmanje istraženom citokinskom familijom sa značajnom ulogom u procesu inflamacije. Ubikvitarna rasprostranjenost receptora za IL-17 omogućava ovom citokinu mnogobrojne biološke efekte u organizmu, poput važne uloge u imunskom odgovoru i regulaciji procesa hematopoeze, ali i u progresiji različitih bolesti, uključujući inflamatorna, autoimunska i maligna oboljenja. IL-17 ima ulogu u ćelijskim procesima poput proliferacije i diferencijacije, i to prvenstveno na hematopoetskim matičnim i progenitor ćelijama, pri čemu IL-17 ispoljava različite efekte na proliferaciju i diferencijaciju zavisno od tipa ćelijske loze i stepena diferencijacije ćelija. Takoñe, zbog ubikvitarne rasprostranjenosti svog receptora, IL-17 je uključen u interakcije izmeñu imunskog sistema i somatskih tkiva. Novija istraživanja su pokazala da IL-17 ima i ulogu finog regulatora diferencijacije mezenhimskih matičnih ćelija (MSC, Mesenchymal stem cells), s obzirom na to da stimuliše osteogenu diferencijaciju dok, s druge strane, inhibira adipogenu diferencijciju humanih MSC. Savremena istraživanja biologije MSC ukazala su na širok opseg njihove potencijalne terapijske primene, prvenstveno u regenerativnoj medicini, jer ove multipotentne adultne matične ćelije koje predstavljaju nediferencirane ćelije sa sposobnošću samoobnove, imaju sposobnost diferenciranja u ćelije tkiva mezenhimskog porekla, poput osteoblasta, hondroblasta i adipocita, a prema saznanjima koja imamo danas, i u ćelije tkiva ektodermalnog i endodermalnog porekla. Matične ćelije različitog stepena multipotentnosti izolovane su iz raznih adultnih tkiva, kao i perinatalnih tkiva koja se poslednjih godina sve češće pominju kao povoljan izvor MSC. Meñutim, i pored velikog napretka u ovoj oblasti, nema konačnih saznanja kako o mehanizmima odgovornim za regulaciju višestrukih funkcija MSC, tako i o njihovom ukupnom terapijskom potencijalu. Kako biološke funkcije MSC umnogome zavise od konteksta mikrosredine, ispitivanje interakcija MSC i IL-17 u održavanju multipotentnosti, mobilizaciji i usmerenoj diferencijaciji MSC je od posebnog značaja. Pored mezenhimskih ćelija, IL-17 ostvaruje svoje efekte delovanjem na endotelske ćelije. Jedan od terapijskih ciljeva u regenerativnoj medicini, koji je još uvek nedovoljno objašnjen, je i podsticanje revaskularizacije oštećenog tkiva. Stimulacija postojećih, in situ prisutnih endotelskih ćelija na angiogenezu je jedan od mogućih mehanizama. Efekti IL-17 na endotelske ćelije nisu do sada u potpunosti opisani, i poznato je samo da IL-17 u prisustvu drugih poznatih angiogenih faktora stimuliše sposobnost endotelskih ćelija za angiogenezu. Pored toga, poznato je da se funkcije ćelija kao i delovanje citokina značajano razlikuju u zavisnosti od procenta kiseonika prisutnog u mikrosredini ćelija. Efekat delovanja IL-17 na endotelske ćelije u prisustvu različitih koncentracija kiseonika do sada nije istraživan. Takoñe je još uvek nepoznato da li IL-17 može uticati na diferencijaciju MSC u endotelske ćelije. Da bi se sprovela istraživanja u ovom pravcu, neophodno je uspostaviti pogodan model za diferencijaciju MSC u endotelske ćelije Cilj istraživanja ove disertacije bilo je ispitivanje efekata IL-17 na različite ćelijske funkcije mezenhimskih i endotelskih ćelija u specifičnim uslovima mikrosredine. Analizirano je dejstvo IL-17 na proliferaciju, migraciju, angiogenezu i diferencijaciju ćelija. Istraživani su i molekularni mehanizmi koji omogućavaju finu regulaciju ćelijskih funkcija od strane IL-17, analizom aktivacije signalnih molekula, kao i ekspresije gena i proteina neophodnih za specifične funkcije. Posebna pažnja u istraživanjima bila je posvećena izolaciji, karakterizaciji i endotelskoj diferencijaciji primarnih MSC iz tkiva pupčanika (UC-MSC, Umbilical cord-mesenchymal stem cells). U okviru razjašnjavanja efekata i mehanizama delovanja IL-17 na diferencijaciju mezenhimskih ćelija, primenjena je multipotentna mišja C2C12 ćelijska linija. Uticaj IL-17 na diferencijaciju analiziran je na osnovu ekspresije gena i proteina specifičnih za odreñeni tip diferencijacije: teškog lanca miozina (MyHC, Myosin heavy chain) i miogenina za miogenu, a alkalne fosfataze (ALP, Alkaline phosphatase), Runx2/Cbfa1 (Runt-related transcription factor 2/Core-binding factor subunit alpha-1) transkripcionog faktora i ciklooksigenaze 2 (Cox-2, Cyclooxygenase 2) za osteogenu diferencijaciju. Pored toga, utvrñivano je učešće mitogenom aktiviranih protein kinaza (MAPK) i morfogenetskih proteina kosti (BMP, Bone morphogenic proteins) u IL-17-usmerenoj diferencijaciji C2C12 ćelija. Rezultati su pokazali da IL-17 inhibira miogenu i stimuliše osteogenu diferencijaciju C2C12 ćelija, s obzirom na to da je IL-17-zavisna inhibicija miogeneze povezana sa redukcijom ekspresije iRNK za miogenin, redukcijom ekspresije MyHC i izostankom formiranja miotuba, dok je IL-17-zavisna indukcija osteogeneze povezana sa indukcijom ekspresije iRNK za Runx2/Cbfa1, indukcijom ekspresije Cox-2 i povišenom aktivnošću ALP. Rezultati su takoñe pokazali da IL-17 ostvaruje ove efekte putem aktivacije ERK1,2 MAPK signalnog puta, nezavisno od BMP-Smad signalnog puta. U drugom delu ove studije ispitivan je efekat IL-17 na funkcije endotelskih ćelija na modelu humane endotelske ćelijske linije, EA.hy 926, praćenjem njihove proliferacije, migracije i tubulogeneze u uslovima normoksije i hipoksije. Analizirana je i ekspresija gena za endotelsku azot monoksid sintazu (eNOS, Endothelial nitric oxide synthase) i Cox-2, kao i njihovih proteinskih produkata, budući da ovi molekuli imaju važnu ulogu u procesu angiogeneze. Dobijeni rezultati su potvrdili da IL-17 stimuliše ključne funkcije uključene u proces angiogeneze EA.hy 926 ćelija, poput ćelijske migracije i tubulogneze, kao i ekspresiju eNOS i Cox-2 u ovim ćelijama. Pro-angiogeni efekti IL-17 su pokazani u uslovima normoksije (20% O2). Uslovi hipoksije (3% O2) pokazali su toksično dejstvo na ovu ćelijsku liniju, dok je IL-17 u ovakvim uslovima umanjio broj apoptotičnih ćelija. U okviru trećeg dela ove disertacije MSC su izolovane iz vezivnog tkiva pupčanika, tzv. Vartonove sluzi, umnožene i okarakterisane prema važećim preporukama i kriterijumima Meñunarodnog društva za ćelijsku terapiju. Zatim je ispitan i odreñen optimalni protokol za njihovu efikasnu diferencijaciju u endotelske ćelije, u cilju uspostavljanja novog ćelijskog modela za buduća istraživanja. Princip diferenciranja UC-MSC u endotelske ćelije zasnivao se na inhibiciji signalizacije faktora transformacije rasta (TGF-β, Transforming growth factor beta), i to njegovog receptora, aktivinu-nalik kinaze 5 (ALK5, Activin-like kinase). Na osnovu dobijenih rezultata, zaključeno je da se UC-MSC odlikuju visokom sposobnošću samoobnove i visokim kapacitetom za diferencijaciju kao i da se mogu diferencirati u endotelske ćelije putem inhibicije ALK5 receptora" ]

[ "Interleukin (IL) 17 cytokine family is considered the least explored cytokine family with an important role in inflammation. Due to the ubiquitous expression of its receptor, IL-17 has been implicated in interactions between the immune system and somatic tissues. In this context, recent findings have shown that IL-17 acts as a fine regulator of mesenchymal stem cell (MSC) differentiation. Having in mind that behaviour of MSC depends on the surrounding microenvironment, it is important to analyze the interactions between MSC and IL-17 involved in the maintenance of their multipotency, mobilization and directed differentiation. In addition to mesenchymal cells, IL-17 exerts its roles by acting on endothelial cells as well. Even though IL-17’s effects on endothelial cells have not been fully discovered yet, it is well known that IL- 17, in the presence of other angiogenic factors, has the ability to stimulate endothelial cells to undergo angiogenesis. Furthermore, it is well known that cellular functions, as well as cytokine effects can be significantly modulated by the percentage of oxygen present in their microenvironment. However, effects of IL-17 on endothelial cells in the context of different O2 concentrations have not been explored yet. Also, it is still unknown whether IL-17 can affect endothelial differentiation of MSC, but to address this issue, it is necessary to create a valid model of endothelial differentiation of MSC. The objective of this study was to investigate the effects of IL-17 on different functions of mesenchymal and endothelial cells in specific microenvironmental conditions. The influence of IL-17 on proliferation, migration, angiogenesis and differentiation was analyzed, as well as the molecular mechanisms involved. The activation of various signaling molecules and the expression of genes and proteins involved in specific functions were examined. Yet another objective was to establish an appropriate model for the mesenchymal-endothelial transdifferentiation. For this task primary MSC from umbilical cord tissue (UC-MSC) were isolated and characterized with emphases on their endothelial differentiation. In order to elucidate the effects and mechanisms involved in IL-17 acting on mesenchymal cell differentiation, a multipotent mouse C2C12 cell line was used. The influence of IL-17 on cell differentiation was analyzed based on the expression of genes and proteins specific for the certain type of differentiation: Myosin heavy chain (MyHC) and Myogenin for myogenic, and Alkaline phosphatase (ALP), Runt-related transcription factor 2/Core-binding factor subunit alpha-1 (Runx2/Cbfa1) and Cyclooxygenase-2 (Cox-2) for osteogenic differentiation. Additionaly, the involvement of Mitogen-activated protein kinases (MAPK) and Bone morphogenetic proteins (BMP) in IL-17-directed differentiation of C2C12 cells was investigated. Results obtained demonstrated that IL-17 inhibits myogenic and stimulates osteogenic differentiation of C2C12 cells, by down-regulating the Myogenin mRNA expression, MyHC expression and myotube formation, while up-regulating the Runx2/Cbfa1 mRNK expression, Cox- 2 expression and ALP activity. IL-17 exerted these effects by activating ERK1,2 MAPK signaling pathway, which in turn regulated the expression of relevant genes and proteins to inhibit myogenic differentiation and induce osteogenic differentiation. It was also shown that the induction of osteogenic differentiation by IL-17 is independent of the BMP-Smad signaling pathway. In the second part of this study the effect of IL-17 on endothelial cell functions was investigated using human endothelial cell line EA.hy 926 as a model. The changes in cells’ proliferation, migration and tubulogenesis were analyzed, along with the expression of genes for endothelial Nitric oxide synthase (eNOS) and Cox-2, and their corresponding protein products, since these molecules have important roles in angiogenesis. The influence of the environmental stimuli (normoxia vs. hypoxia) was also investigated. Results obtained demonstrated that IL-17 stimulates crutial events involved in angiogenesis of EA.hy 926 cells, such as cell migration and tubulogenesis, along with the expression of eNOS and Cox-2. However, the effects of IL-17 were dependent on the O2 concentration, since pro-angiogenic effects of IL-17 were noticed in the presence of 20% O2, while 3% O2 exerted toxic effect on this cell line, and IL-17 in these conditions decreased the number of apoptotic cells. In the final task, MSC were isolated from the umbilical cord Wharton’s jelly, expanded in culture and characterized according to the recomendations from the International Society for Cell Therapy. Following this, an optimal protocol for the endothelial diffrentiation of UC-MSC was established. The endothelial-directed differentiation was based on the inhibition of the Transforming growth factor beta (TGF-β) signaling, specifficaly its receptor activin-like kinase 5 (ALK5). Based on the results gained, it was concluded that UC-MSC have a high ability to selfrenew and high diferentiation capacity, as well as that these cells can be differentiated into endothelial cells by inhibiting ALK5 receptor" ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175062/RS//", "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175062/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "Interleukin-17", "mezenhimske matične ćelije", "endotelske ćelije", "diferencijacija", "angiogeneza", "C2C12", "EA.hy 926", "tkivo pupčanika", "hipoksija" ]

[ "Interleukin-17", "mesenchymal stem cells", "endothelial cells", "differentiation", "angiogenesis", "C2C12", "EA.hy 926", "umbilical cord", "hypoxia" ]

[ "Efekti i mehanizmi delovanja interleukina-17 na funkcije mezenhimskih i endotelskih ćelija", "Effects of interleukin-17 on mesenchymal and endothelial cell functions and mechanisms involved" ]

[ "" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/1898/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1898/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2060" ]

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[ "Kotur, Nikola" ]

[ "Matić, Gordana", "Radović, Svetlana", "Zukić, Branka", "Dokmanović, Lidija" ]

[ "2019-11-15T10:03:58", "2020-07-03T08:07:53" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/11509", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6926", "https://fedorabg.bg.ac.rs/fedora/get/o:20286/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025219506" ]

[ "Personalizovana medicina, medicina XXI veka, nastoji da individualizuje terapiju za svakog pacijenta, kako bi lečenje bilo što efikasnije i sa što manje rizika od toksičnosti. Personalizovana medicina se danas najviše oslanja na farmakogenomiku i farmakotranskriptomiku, koje su već dale svoj doprinos unapređenju lečenja mnogih bolesti, a posebno maligniteta. Akutna limfoblastna leukemija (ALL) predstavlja najčešći hematološki malignitet pedijatrijskog uzrasta. Mada stopa izlečenja dostiže 80-90%, ALL je i dalje glavni uzrok mortaliteta u ovih pacijenata. Terapija izaziva neželjene efekte u 75% pacijenata. Pored toga, u 1-3% pedijatrijskih pacijenata sa ALL smrtni ishod nije posledica bolesti, već je uzrokovan terapijom. Lečenje pedijatrijske ALL nije napredovalo uvođenjem novih lekova, već nastojanjem da se smanje neželjena dejstva onih lekova koji su već sastavni delovi postojećih terapijskih protokola, zbog čega su farmakogenomika i farmakotranskriptomika dobile ključno mesto u ovoj oblasti. Glukokortikoidni lekovi (GK) se koriste u početnoj fazi lečenja ALL u dece, u fazi indukcije remisije. Od početka lečenja do 8. dana se primenjuju isključivo GK. Ipak, farmakogenomske i farmakotranskriptomske studije za glukokortikoidne lekove još uvek nisu dovele do algoritma koji bi mogao biti primenjen u lečenju dečje ALL. Stoga je izuzetno značajno nastaviti sa istraživanjima farmakogenomskih i farmakotranskriptomskih markera relevantnih za uspešnost GK terapije u dece sa ALL. Razumevanje molekularnog mehanizma dejstva GK vodi ka otkrivanju novih markera koji mogu biti iskorišćeni za optimizaciju GK terapije. Nove tehnologije, kao što je sekvenciranje nove generacije (eng, next generation sequencing, NGS) su omogućile dizajniranje panela za farmakogenomske i farmakotranskriptomske markere za različite lekove..." ]

[ "Personalized medicine, the medicine of the XXI century, aims to individualize therapy for each patient, in order for the treatment to be as efficient and safe as possible. Today, personalized medicine is the most reliant on pharmacogenomics and pharmacotranscriptomics, which have already given their contribution to enhancing treatment of many diseases, especially malignancies. Acute lymphoblastic leukemia (ALL) is the most common hematological malignancy of childhood. Even though the percentage of cured patients reaches 80-90%, ALL is still the main cause of mortality in this group of patients. Therapy causes side effects in 75% of patients. Aside from that, 1-3% of pediatric ALL patients die because of therapy side effects rather than the disease itself. Treatment of pediatric ALL wasn’t improved by the introduction of new drugs, but by decreasing the side effects of the drugs which are already included in existing protocols. Due to this fact, pharmacogenomics and pharmacotranscriptomics have gained key positions in this field. Glucocorticoid drugs (GC) are used in the initial phase of childhood ALL treatment, in the phase of remission induction therapy. From the beginning of the treatment until day 8, GCs are exclusively applied. Pharmacogenomic and pharmacotranscriptomic studies for GC drugs have yet to produce an algorithm that could be applied in childhood ALL treatment. Therefore, it is of extreme importance to continue researching pharmacogenomic and pharmacotranscriptomic markers relevant to the success of the GC therapy of children with ALL. Understanding the molecular mechanism of action of GC can lead to discovery of new markers that could be used for the optimization of GC therapy. New technologies, such as next generation sequencing (NGS) have created a possibility for designing panels for pharmacogenomic and pharmacotranscriptomic markers of response to different drugs. Utilization of these panels in population pharmacogenomic studies can lead to new knowledge that could open wide the doors to predictive pharmacogenomic testing..." ]

[ "info:eu-repo/grantAgreement/EC/FP7/316088/EU//", "info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41004/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "farmakogenetika", "farmakotranskriptomika", "populaciona farmakogenomika", "pedijatrijska akutna limfoblastna leukemija", "glukokortikoidni lekovi", "GAS5", "NF-κB", "sekvenciranje nove generacije" ]

[ "pharmacogenetics", "pharmacotranscriptomics", "population pharmacogenomics", "pediatric acute lymphoblastic leukemia", "glucocorticoid drugs", "GAS5", "NF-κB", "next generation sequencing" ]

[ "Farmakogenomski i farmakotranskriptomski markeri odgovora na terapiju glukokortikoidnim lekovima kod dece sa akutnom limfoblastnom leukemijom: molekularni mehanizmi delovanja, klinički i populacioni aspekti" ]

[ "Pharmacogenomic and pharmacotranscriptomic markers in glucocorticoid treatment of pediatric acute lymphoblastic leukemia: molecular mechanism of action, clinical and population aspects" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/1719/IzvestajKomisije21120.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1718/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1719/IzvestajKomisije21120.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1718/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_11509" ]

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[ "Smederevac, Snežana" ]

[ "Biro, Mikloš", "Čolović, Petar", "Tenjović, Lazar", "Smederevac, Snežana" ]

[ "2017-05-31T12:22:55", "2020-07-03T14:22:57" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/8121", "http://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija140549416002562.pdf?controlNumber=(BISIS)89538&fileName=140549416002562.pdf&id=2461&source=NaRDuS&language=sr", "http://www.cris.uns.ac.rs/record.jsf?recordId=89538&source=NaRDuS&language=sr", "http://www.cris.uns.ac.rs/DownloadFileServlet/IzvestajKomisije140549450872631.pdf?controlNumber=(BISIS)89538&fileName=140549450872631.pdf&id=2463&source=NaRDuS&language=sr" ]

[ "Ispitivanje prikazano u radu problematizira relacije između ličnosti posmatrane sa tipološke perspektive i ideologije obuhvaćene preko sistema vrijednosti, društvenih stavova i religioznih uvjerenja. Istraživanje je provedeno na uzorku od 1046 ispitanika (65% žena), starosti 18-74 godine (prosječna starost 32,74 godine). U ispitivanju su korišteni sljedeći instrumenti: upitnik ličnosti Velikih pet plus dva (VP+2), Švarcov upitnik vrijednosti (PVQ), Ajzenkova skala društvenih stavova, Skala religioznih uvjerenja i Skala polariteta. Klasterizacija upitnika VP+2 dovela je do kategorizacije ličnosti na: hiperkontrolisani, rezilijentni i hipokontrolisani tip. Rezultati su pokazali da se za svaki od tipova ličnosti veže specifičan vrijedonosni profil. Rezilijentni tip ličnosti se uglavnom veže za klaster vrijednosti Otvorenost za promjene (Nezavisnost, Poticaj) i Sopstveno odricanje (Univerzalnost, Dobronamjernost), dok se hipokontrolisani tip uglavnom veže za klaster vrijednosti Samounapređenja (Postignuće, Moć). Oko 40% varijabiliteta vrijednosti može se objasniti preko tipova ličnosti. Društveni stavovi Etnocentrizam i Liberalizam slabo su povezani sa tipovima ličnosti. Isto je konstatovano i za religiozna uvjerenja i normativno-humanističku orijentaciju. U osnovi, ličnost posmatrana preko tipova ili preko dimenzija, nije se pokazala kao značajan prediktor političkog ponašanja. Društveni stavovi i vrijednosti takođe su skromni prediktori političkog ponašanja, s tim da je prognoza nešto bolja u slučaju predikcije glasanja za stranke desnog centra (SDA), gdje je moguće objasniti oko 20% varijanse glasačkog ponašanja." ]

[ "This study discusses relation between personality from aspects of typological perspective and ideology determined as system of values, social attitudes and religious beliefs. The research was conducted on sample of 1046 respondents (65% female), aged from 18 to 74 years (average age 32,74 years). The instruments administrated in research were: Big Five plus Two (Velikih pet plus dva, VP+2), Schwartz Value Survey (PVQ), Eysenck’s Inventory of Social Attitudes, Religious Beliefs Scale and Polarity Scale. Clustering of Big Five plus Two (VP+2) inventory led to the categorization of personality to overcontrolled, undercontrolled and resilient type. The results showed that specific value profile is related to each type of personality. Resilient type is mostly related to the value cluster Openness to Change (Self direction and Stimulation) and Self-Transcendence (Universalism and Benevolence), while undercontrolled type is mostly related to value cluster Self-Enhancement (Power and Achievement). About 40% variance of values could be explained through personality types. Social attitudes Ethnocentrism and Liberalism are weakly associated with personality types. The same relation could be stated to religious beliefs and normative-humanistic orientation with personality types. Basically, personality operationalized through types or through dimensions either, has not showed as significant predictors of political behavior. Social attitudes and values are modest predictors of political behavior, although the prediction is somewhat better in case of prediction for right-wing parties (SDA), where is possible to explain about 20% of variance of political behavior" ]

[ "" ]

[ "openAccess" ]

[ "" ]

[ "Tipovi ličnosti", "ideologija", "vrijednosti", "društveni stavovi", "političko ponašanje" ]

[ "Personality types", "ideology", "values", "social attitudes", "political behavior" ]

[ "Tipološki pristup dimenzijama ideološke orijentacije" ]

[ "Typological approach to dimensions of ideological orientation" ]

[ "ARR" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/45908/Disertacija9336.pdf", "https://nardus.mpn.gov.rs/bitstream/id/45909/IzvestajKomisije9336.pdf", "http://nardus.mpn.gov.rs/bitstream/id/45908/Disertacija9336.pdf", "http://nardus.mpn.gov.rs/bitstream/id/45909/IzvestajKomisije9336.pdf", "http://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija140549416002562.pdf?controlNumber=(BISIS)89538&fileName=140549416002562.pdf&id=2461&source=NaRDuS&language=sr" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_8121" ]

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[ "Milošević, Vesna" ]

[ "Lozo, Jelena", "Velhner, Maja", "Golić, Nataša", "Strahinić, Ivana" ]

[ "2016-01-05T11:45:30", "2020-07-03T08:08:23" ]

[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=10", "https://nardus.mpn.gov.rs/handle/123456789/2036", "https://fedorabg.bg.ac.rs/fedora/get/o:2131/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=" ]

[ "Salmoneloze spadaju među najznačajnije zoonoze u svetu. S obzirom da se ljudi zaražavaju najčešće putem konzumacije hrane životinjskog porekla, nadzor nad salmonelama neophodan je u svakom stadijumu proizvodnje hrane od farme do viljuške. Širenje rezistencije na hinolone među salmonelama predstavlja veliki javno zdravstveni problem zbog uticaja na lečenje ljudi i životinja. Cilj istraživanja je bio da se ispita mogućnost brze i efikasne tipizacije Salmonella enterica subspecies enterica serotip Enteritidis (S. Enteritidis) kao najčešćeg serotipa kod ljudi i učestalost i molekularni mehanizmi rezistencije na hinolone kod salmonela. U kolekciji izolata S. Enteritidis poreklom od ljudi, pilića i iz namirnica živinskog porekla, kombinacijom RAPD-PCR tipizacije sa četiri prajmera, rezistotipizacije i polimorfizma mutacija u regionu gyrA gena koji kodira rezistenciju na hinolone (QRDR), diskriminisano je 22 genetičke grupe sa indeksom diskriminacije 0.828. Svi izolati bili su visoko srodni, a tipizacija je dokazala nasumičnu raspoređenost tipova u sve tri kategorije izolata, odnosno cirkulaciju sojeva kroz razne stadijume lanca ishrane. RAPD-PCR tipizacija uspešno je izdvojila epidemijski soj među tri izolata S. Enteritidis iz jednog restorana brze hrane. Analizom uzoraka iz fabrike biskvita ustanovljena je kontaminacija sa najmanje četiri soja S. Enteritidis i povezanost serotipa Enteritidis sa jajima kao izvorom kontaminacije. Analizom većeg uzorka izolata poreklom od namirnica i ljudi sa teritorije Južnobačkog okruga u šestogodišnjem periodu ustanovljena je mala učestalost rezistencije S. Enteritidis na antibiotike, retka pojava višestruke rezistencije na antibiotike i mala učestalost rezistencije na hinolone (2.9%). Nađena su tri tipa mutacija odgovornih za rezistenciju na hinolone u QRDR regionu: Asp87Asn, Asp87Gly i Ser83Phe. Dominirale su mutacije na 87. poziciji i to Asp87Asn supstitucija. Nisu nađeni dokazi o klonalnom širenju sojeva rezistentnih na nalidiksičnu kiselinu. Rezistencija na fluorohinolone nije otkrivena. Dokazana je smanjena osetljivost na fluorohinolone kod S. Enteritidis rezistentnih na nalidiksičnu kiselinu. Kod drugih serotipova salmonela rezistencija na nalidiksičnu kiselinu i udruženost sa više markera rezistencije bila je učestalija nego kod S. Enteritidis, naročito kod S. Hadar. Rezistencija na ampicilin i nalidiksičnu kiselinu bile su najčešće kod salmonela bez obzira na serotip. Diskriminativna moć i jednostavnost RAPD-PCR metode sa četiri prajmera udružene sa rezistotipizacijom preporučuju ih kao efikasan sistem tipizacije prilikom nadzora nad kretanjem salmonela u humanoj i životinjskoj populaciji. Ispitivanje rezistencije na antibiotike, a naročito na hinolone kod salmonela neophodno je u cilju upozoravanja kliničara na terapijske rizike, a sa epidemiološkog aspekta, radi detekcije pojave i širenja opasnih sojeva." ]

[ "Salmonelloses are among most significant zoonoses in the world. Since people are most often infected by consumption of food of animal origin, surveillance of salmonella is necessary in every stage of food production from farm to fork. Emergence of resistance to quinolones among salmonella is a significant public health problem, considering implications on the treatment of humans and animals. The aims of the investigation were to analyze the possibility of fast and efficient typing of Salmonella enterica subspecies enterica serotype Enteritidis (S. Enteritidis) as the most frequent serotype in humans and to investigate frequency and molecular mechanisms determining resistance to quinolones in salmonella. In a collection of S. Enteritidis isolates originating from humans, poultry and marketed poultry products and eggs, a combination of RAPD-PCR typing with four primers, resistotyping and polymorphism of mutations in quinolone resistance determining region (QRDR) of gyrA gene revealed 22 genetic groups with discrimination index of 0.828. All isolates were closely related and typing has proved random distribution of types in all three categories of isolates, ie. circulation of strains through subsequent stages of food chain. RAPD-PCR typing succesfully discriminated epidemic strain among 3 S. Enteritidis isolates from one fast food restaurant. Analysis of samples from one biscuit factory showed contamination with at least four strains of S. Enteritidis and strong connection of serotype Enteritidis with eggs as a source of contamination. Analysis of larger collection of isolates from food and humans in Southern Bačka county in a six-year period revealed low frequency of resistance of S. Enteritidis to antibiotics, rare occurence of multiple resistance to antibiotics and low frequency of resistance to quinolones (2.9%). Three types of mutations determining resistance to quinolones in QRDR were discovered: Asp87Asn, Asp87Gly and Ser83Phe. Mutations at position 87 are dominant, with Asp87Asn as the most frequent. There was no evidence of clonal spread of strains resistant to quinolones. Resistance to fluoroquinolones was not detected. Reduced susceptibility to fluoroquinolones among S. Enteritidis resistant to nalidixic acid was proved. Resistance to nalidixic acid and association with multiple antibiotic resistance were more common among other serotypes of salmonella, especially in S. Hadar, than among S. Enteritidis. Resistance to ampicillin and nalidixic acid were the most common in all investigated salmonella serotypes. Discriminative power and simplicity of RAPD-PCR method with four primers together with resistotyping recommend them as the efficient typing system for monitoring of salmonella in human and animal population. Investigation of resistance to antibiotics, especially to quinolones among salmonella is essential for alerting clinicians for therapy risks, and from epidemiological aspect, for detection of emergence and spread of dangerous strains." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173019/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "Salmonella Enteritidis", "RAPD", "rezistencija", "hinoloni", "gyrA", "QRDR" ]

[ "Salmonella Enteritidis", "RAPD", "resistance", "quinolones", "gyrA", "QRDR" ]

[ "Analiza prirode rezistencije na hinolone i molekularna tipizacija odabranih serotipova Salmonella enterica subspecies enterica", "Analysis of nature of resistance to quinolones and molecular typing of selected serotypes of Salmonella enterica subspecies enterica" ]

[ "" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/1878/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1878/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2036" ]

[ "" ]

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[ "Pavić, Dragoslav" ]

[ "Plavša, Jovan", "Pavić, Dragoslav", "Dolinaj, Dragan", "Garača, Vuk", "Milanović, Miško" ]

[ "2015-12-29T11:16:17", "2020-07-03T13:41:59" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/1657", "http://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija144707854589318.pdf?controlNumber=(BISIS)95358&fileName=144707854589318.pdf&id=4563&source=NaRDuS&language=sr", "http://www.cris.uns.ac.rs/record.jsf?recordId=95358&source=NaRDuS&language=sr", "http://www.cris.uns.ac.rs/DownloadFileServlet/IzvestajKomisije143583115827360.pdf?controlNumber=(BISIS)95358&fileName=143583115827360.pdf&id=3896&source=NaRDuS&language=sr" ]

[ "У овој дисертацији су студиозно сагледане могућности унапређења туристичке понуде северозападне Црне Горе(општине Жабљак, Пљевља, Плужине, Шавник) на бази недовољно искоришћених природних туристичких вредности. Управо разноврстан, хоризонтално и вертикално рашчлањен рељеф и веома сложен геолошки састав, специфичне климатске карактеристике, потом реке и бројна планинска језера, те богат биљни и животињски свет, представљају оне природне вредности које потенцијално чине основу унапређења туристичке понуде овог дела Црне Горе базираног и на заштићеним природним добрима(Национални парк„Дурмитор“, Регионални парк„Пива“). Посебан акценат је дат у сагледавању и оцени нивоа искоришћености природних туристичких вредности, као примарних туристичких мотива у границама истраживаног простора. Истовремено су уз процену и вредновање ових ресурса предложене поједине активности за њихово квалитетније уклапање у туристичку понуду северозападне Црне Горе. Реализација таквих активности би требала утицати на унапређење туристичке понуде које се може постићи, генерално само развојем туризма који је у сагласности са еколошким нормама, нарочито ако се имају у виду постојећа заштићена природна добра на територији подручја. Такође је у разматрању наведене проблематике презентовано актуелно стање постојеће укупне туристичке понуде истраживаног подручја која укључује или би требала укључивато поједине антропогене вредности. Оне практично нису укључене у понуду локалних туристичких агенција, с обзиром на то да су агенције готово искључиво усмерене на програме валоризације природних вредности(рафтинг, планинарење и др.). Ове вредности јесу основа унапређења туристичке понуде подручја, али су антропогене вредности њена потенцијално адекватна допуна која уз одговарајуће активности надлежних субјеката може утицати једним делом на садржајну диверзификацију туристичке понуде подручја. SWOT анализом се кроз њене саставне факторе указало на значајне претпоставке у формирању туристичке понуде истраживаног подручја, као што се указало и на прагове ограничења, али и на могућности које могу послужити за умањење или превазилажење неких од њих. За реализацију тих могућности је потребна свест локалне заједнице о томе да се резултати појединих негативних процеса на делу подручја (изразити пример НП„Дурмитор“) који су мотивисани краткорочним потребама морају са аспекта квалитета природних вредности, одразити на исте кроз дугорочне импликације. Осим овом анализом, значајна сазнања су добијена и из анкетног истраживања спроведеног на узорку од200 случајно изабраних туриста на више локација унутар подручја. Резултати овог истраживања су послужили као веома валидан индикатор предности и недостатака актуелне туристичке понуде, али и нивоа задовољства анкетираних туриста чиме је такође указано на смернице унапређења појединих сегмената туристичке понуде. Наглашеним апликативним карактером овај део истраживања пружа одговарајући информативни допринос надлежним туристичким субјектима којим се указује на анкетним истраживањем утврђен степен афирмативности места, односно подручја у целини. Истовремено, резултати поменутог истраживања пружају, између осталог, и значајне информације које се односе на остварен квалитет, као и на израженије преферирање неких сегмената туристичке понуде при чему исто може послужити делом и као полазна основа у прилагођавању појединих сегмената понуде потребама туриста. Истраживањем у оквиру израде дисертације је констатоватована присутност одређене дискоординације услед различитости у нивоима надлежности и неуједначености у погледу централизације/децентрализације управљања над појединим организационим субјектима. У вези са тим, а на основу резултата проистеклих из целокупно спроведеног истраживања кроз интердисциплинарни приступ дошло се до сагледавања потребе реализације више конкретних мера међу којима је једна од њих потреба формирања Регионалне туристичке организације што је једна од предиспозиција које су од значаја у просецу бољег позиционирања истраживаног подручја као туристичке дестинације.", "U ovoj disertaciji su studiozno sagledane mogućnosti unapređenja turističke ponude severozapadne Crne Gore(opštine Žabljak, Pljevlja, Plužine, Šavnik) na bazi nedovoljno iskorišćenih prirodnih turističkih vrednosti. Upravo raznovrstan, horizontalno i vertikalno raščlanjen reljef i veoma složen geološki sastav, specifične klimatske karakteristike, potom reke i brojna planinska jezera, te bogat biljni i životinjski svet, predstavljaju one prirodne vrednosti koje potencijalno čine osnovu unapređenja turističke ponude ovog dela Crne Gore baziranog i na zaštićenim prirodnim dobrima(Nacionalni park„Durmitor“, Regionalni park„Piva“). Poseban akcenat je dat u sagledavanju i oceni nivoa iskorišćenosti prirodnih turističkih vrednosti, kao primarnih turističkih motiva u granicama istraživanog prostora. Istovremeno su uz procenu i vrednovanje ovih resursa predložene pojedine aktivnosti za njihovo kvalitetnije uklapanje u turističku ponudu severozapadne Crne Gore. Realizacija takvih aktivnosti bi trebala uticati na unapređenje turističke ponude koje se može postići, generalno samo razvojem turizma koji je u saglasnosti sa ekološkim normama, naročito ako se imaju u vidu postojeća zaštićena prirodna dobra na teritoriji područja. Takođe je u razmatranju navedene problematike prezentovano aktuelno stanje postojeće ukupne turističke ponude istraživanog područja koja uključuje ili bi trebala uključivato pojedine antropogene vrednosti. One praktično nisu uključene u ponudu lokalnih turističkih agencija, s obzirom na to da su agencije gotovo isključivo usmerene na programe valorizacije prirodnih vrednosti(rafting, planinarenje i dr.). Ove vrednosti jesu osnova unapređenja turističke ponude područja, ali su antropogene vrednosti njena potencijalno adekvatna dopuna koja uz odgovarajuće aktivnosti nadležnih subjekata može uticati jednim delom na sadržajnu diverzifikaciju turističke ponude područja. SWOT analizom se kroz njene sastavne faktore ukazalo na značajne pretpostavke u formiranju turističke ponude istraživanog područja, kao što se ukazalo i na pragove ograničenja, ali i na mogućnosti koje mogu poslužiti za umanjenje ili prevazilaženje nekih od njih. Za realizaciju tih mogućnosti je potrebna svest lokalne zajednice o tome da se rezultati pojedinih negativnih procesa na delu područja (izraziti primer NP„Durmitor“) koji su motivisani kratkoročnim potrebama moraju sa aspekta kvaliteta prirodnih vrednosti, odraziti na iste kroz dugoročne implikacije. Osim ovom analizom, značajna saznanja su dobijena i iz anketnog istraživanja sprovedenog na uzorku od200 slučajno izabranih turista na više lokacija unutar područja. Rezultati ovog istraživanja su poslužili kao veoma validan indikator prednosti i nedostataka aktuelne turističke ponude, ali i nivoa zadovoljstva anketiranih turista čime je takođe ukazano na smernice unapređenja pojedinih segmenata turističke ponude. Naglašenim aplikativnim karakterom ovaj deo istraživanja pruža odgovarajući informativni doprinos nadležnim turističkim subjektima kojim se ukazuje na anketnim istraživanjem utvrđen stepen afirmativnosti mesta, odnosno područja u celini. Istovremeno, rezultati pomenutog istraživanja pružaju, između ostalog, i značajne informacije koje se odnose na ostvaren kvalitet, kao i na izraženije preferiranje nekih segmenata turističke ponude pri čemu isto može poslužiti delom i kao polazna osnova u prilagođavanju pojedinih segmenata ponude potrebama turista. Istraživanjem u okviru izrade disertacije je konstatovatovana prisutnost određene diskoordinacije usled različitosti u nivoima nadležnosti i neujednačenosti u pogledu centralizacije/decentralizacije upravljanja nad pojedinim organizacionim subjektima. U vezi sa tim, a na osnovu rezultata proisteklih iz celokupno sprovedenog istraživanja kroz interdisciplinarni pristup došlo se do sagledavanja potrebe realizacije više konkretnih mera među kojima je jedna od njih potreba formiranja Regionalne turističke organizacije što je jedna od predispozicija koje su od značaja u prosecu boljeg pozicioniranja istraživanog područja kao turističke destinacije." ]

[ "The dissertation deals with the advancement possibilities of tourist offer in north western part of Montenegro (municipalities of Žabljak, Pljevlja, Plužine, Šavnik) based on insufficiently utilised natural tourism values. Natural tourism values such as diverse, horizontally and vertically segmented relief, extremely complex geological formation, specific climate features, rivers and numerous mountain lakes, bio-diversity of flora and fauna form the prospective ground for tourism offer advancement in this part of Montenegro jointly with protected natural assets (National Park Durmitor and Regional Park Piva). There is special emphasis on comprehension and evaluation of utilization level of natural tourism assets, as primary tourist motives within the boundaries of the researched area. Simultaneous to estimation and evaluation of the resources, certain activities have been proposed for their qualitative integration into the tourist offer of north western part of Montenegro. Realisation of the activities should impact the tourist offer advancement that can be achieved only by means of tourism development that is in concordance with ecology principles, especially related to existing natural assets of this area. Moreover, the study of the issues describes the current condition of the total tourist offer which implies or should imply certain anthropogenic values of the researched area. In practice anthropogenic values were not included into the offer of the local travel agents. Instead, there was exclusive orientation towards the valorisation programmes of natural values (rafting, mountaineering, etc.). Natural values do create the basis for tourist offer advancement in the area, but anthropogenic values create prospective adequate complement to the tourist offer which together with appropriateactivities of the subjects in charge may in return partly impact the content diversification of the tourist offer in the area. SWOT analysis by means of its constituent factors specifies the presuppositions in tourist offer creation of the researched area, identifies restriction factors and also the possibilities that may help to alleviate or surpass some of them. Furthermore, the realisation of the possibilities demands higher awareness of the local community on the issue that the results of certain negative processes in the part of the researched area (prominent example National Park Durmitor) motivated by short-termed needs must have long-termed impact on natural values in terms of quality. Apart from this analysis, important findings have been obtained by the questionnaire conducted on the sample of 200 randomly selected tourists on several locations within the area. The results of the research serve as a valid indicator of strengths and weaknesses of the current tourist offer and the level of satisfaction of the respondents, which also indicates the directions for future improvement of certain segments within the tourist offer. This part of the research offers appropriate informative contribution to the tourism subjects by means of its highlighted applicative potential and defined level of affirmative features of the total area. At the same time the results of the research offer significant information on the achieved quality level and preferred segments of tourist offer that may partly serve as the basisfor adaptation of certain segments within the offer to tourist needs. The research conducted for doctoral thesis highlighted certain discoordination due to differences between the levels of responsibility and unevenly entralised/decentralised management of organisational subjects. Related to this and based on the results of the research based on interdisciplinary approach several measures have been highlighted, among which the need to form Regional tourist organisation as one of the predispositions for the process of better positioning of the researched area as a tourist destination." ]

[ "" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "северозападна Црна Гора", "severozapadna Crna Gora", "природне вредности", "туристичка понуда", "prirodne vrednosti", "turistička ponuda" ]

[ "northwestern Montenegro", "natural values", "tourist offer" ]

[ "Природне вредности као основа унапређења туристичке понуде северозападне Црне Горе", "Prirodne vrednosti kao osnova unapređenja turističke ponude severozapadne Crne Gore", "Natural values as the basis of tourism in north western Montenegro" ]

[ "" ]

[ "BY-NC-ND" ]

[ "http://nardus.mpn.gov.rs/bitstream/id/37808/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/37808/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/37809/IzvestajKomisije.pdf", "http://nardus.mpn.gov.rs/bitstream/id/37809/IzvestajKomisije.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_1657" ]

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[ "Borozan, Igor" ]

[ "Živković, Predrag", "Merenik, Lidija", "Gnjatović, Dragana", "Marković, Predrag" ]

[ "2022-05-31T09:40:19" ]

[ "https://eteze.bg.ac.rs/application/showtheses?thesesId=8614", "https://fedorabg.bg.ac.rs/fedora/get/o:25798/bdef:Content/download", "https://plus.cobiss.net/cobiss/sr/sr/bib/67232777", "https://nardus.mpn.gov.rs/handle/123456789/20481" ]

[ "У расправама теоретичара из различитих научних области, тема интерпретације визуелног идентитета на новцу све је присутнија. Међу економистима и стручњацима из сродних дисциплина, наглашава се међусобни однос између културе, друштава и економије – „непоправљиво испреплетених неодвојивих области“–. Поједине представе на анализираним ликовним решењима, касније и на оптицајном новцу су истовремено допринеле изградњи перцепције становништва у различитим државним трансформацијама у Србији. Помирење дуалне природе новца тежак је задатак који захтева даља питања у вези са фундаменталним значењем новца. Које су се то поруке преносиле путем папирног и кованог новца и у којим је друштвено – економским праксама он циркулисао? Пропаганда ових симбола, кроз разне жанрове илуструје и конзервативну природу „монетарне културе“, што није велико изненађење, али указује и на начине на које је иконографија папирног и кованог новца учествовала у визуелној конструкцији и јачању друштвених идентитета и сврставњу Србије у Европске и светске токове. Дискусија о формирању „монетарне културе“, а уједно и њене безбедносне улоге, начину на који се креира, прво на ликовним решењима за новчанице, затим и на издатом новцу у истраженом периоду од 1868-2018, заснива се на њиховој усклађености и једнакости. Активности по питању обликовања, израде и борбе против фалсификовања новца у Србији су биле веома интензивне и оне представљају један од предмета истраживања у овом раду. У обновљеној Кнежевини Србији, затим, Краљевини Србији у оптицају је био страни новац све до 1868. године, када се појављује први бакарни српски новац кнеза Михаила Обреновића. Сребрни новац уведен је 1875, а златни 1879. године за време Милана Обреновића. Готово све стране монете (43 врсте) фалсификовале су се одмах по пуштању у оптицај. У Кнежевини Србији, у настојању да се оствари контрола технолошког поступка израде националне монете и истовремено спречи фалсификовање новца свих врста и порекла, именовани су „испитивачи руда и фаличног новца“, хемичар Михајло Рашковић, Сима Лозанић и касније Марко Леко од 1884. до 1920. године. Од оснивања Привилеговане народне банке Краљевине Србије (1884) у оптицају је и српски новац – валута динар. Посебно се истичу периоди када је Банка била, у својим почецима и раној фази, везана за стручњаке и гравере Banque de France и Banque nationale de Belgique. Временом је Народна Банка, мудрим укључивањем српских и југословенских научника и уметника успела да оствари визуелну самосталност и изразитост визуелног идентитета, који одражава и континуитет и данас. Томе је свакако допринело укључивање највећих стручњака за ликовну уметност у Управни одбор и Комисију за сарадњу са уметницима Народне банке – Михаила Валтровића, Уроша Предића, Ђорђа Ђоке Јовановића и Паје Јовановића, Рудолфа Арчибалда Рајса, Младена Јосића, Васе Поморишца и других, који су путем позива или конкурса укључили плејаду уметника и научника у рад за ликовна решења, а затим за оптицајни новац. У процесу развоја токова визуелног идентитета на новцу може се издвојити шест периода: крај XIX века и почетак XX века, период између два рата, период после Другог светског рата, период Социјалистичке изградње, Распад II Југославије и период од 2003. до 2018. године. У процесу развоја токова у оквиру визуелног идентитета, прати се хронолошки след догађаја у оквиру надлежности и функција Народне банке. Анализирана идејна решења за новчанице потичу из збирке Народне Банке Србије, односно из Завода за израду новчаница и кованог новца у Топчидеру. У овом раду се сагледавају уметничко-технолошки аспекти и токови развоја визуелног идентитета у служби заштите новца од фалсификовања. viii Поред тога, развој науке и технологије омогућавао је примену савременијих поступака производње и унапређење система заштите новца, а истовремено и веће техничко-технолошке могућности за израду фалсификата. У другом делу овог рада представљен је потенцијал примене Оптичке микроскопије и Раманове спектроскопије као недеструктивних форензичких метода у анализи историјских и оптицајних новчаница. Циљ истраживања јесте доказати да су Оптичка микроскопија и Раманова спектроскопија успешно примењиве у анализи новчаница. Утврђене су сигурносне технике штампе и сигурносни елементи у оквиру уобличавања визуелног идентитета, односно монетарне културе. Експериментални део рада извршен је на историјским новчаницама које су издате у периоду од 1893. до 2018. године." ]

[ "In discussions of theoreticians from various scientific fields, the issue of interpretation of visual identity on money is increasingly present. Economists and experts from similar disciplines are underlining the mutual relation between culture, societies and economy – “irreparably intertwined areas”–. Some illustrations on analysed design solutions, and later on circulating money, also helped build the perception of residents in various state transformations in Serbia. To reconcile the dual nature of money is a difficult task that requires further questions into the fundamental meaning of money. What are the messages that were conveyed by way of paper banknotes and coins, and in which socio-economic practices did such money circulate? The propaganda of these symbols through various genres also illustrates a conservative nature of the “monetary culture”, which is no big surprise, but it does indicate the ways in which the iconography of banknotes and coins participated in the visual construction and strengthening of social identities, and helped make Serbia part of European and global trends. The discussion about the formation of a “monetary culture”, and at the same time its security role, the manner of creation, first in artistic design solutions for banknotes and then on issued money in the researched period 1868–2018, is based on their harmonisation and equivalency. Activities in terms of shaping, producing and fighting against counterfeit money in Serbia were very intensive and are one of the topics of research in this paper. In the renewed Principality of Serbia, and later the Kingdom of Serbia, foreign money was in circulation until 1868, when the first copper Serbian money of Prince Mihailo Obrenović appeared. Silver money was introduced in 1875, and gold in 1879, during Milan Obrenović. Counterfeits of almost all foreign legal tenders (43 kinds) appeared immediately after money was released into circulation. In the Principality of Serbia, in an effort to establish control of the technological process of manufacturing a national legal tender and simultaneously prevent counterfeits of money of all kinds and origins, “examiners of ores and flawed money” were appointed – chemist Mihajlo Rašković, Sima Lozanić and later Marko Leko, from 1884 until 1920. Since the establishment of the Privileged National Bank of the Kingdom of Serbia (1884), Serbian money has also been in circulation – the dinar. Of particular significance are periods when in its beginnings and the early stage, the Bank had close ties with experts and engravers from Banque de France and Banque nationale de Belgique. Over time, by wisely involving Serbian and Yugoslav scientists and artists, the National Bank managed to achieve visual independence and a particular visual identity, which has been maintained in continuity to date. This was certainly facilitated by the involvement of major arts experts in the National Bank’s Governing Board and the Committee for Cooperation with Artists – Mihailo Valtrović, Uroš Predić, Đorđe Đoka Jovanović and Paja Jovanović, Rodolphe Archibald Reiss, Mladen Josić, Vasa Pomorišac and others. By invitation or a competition, these artists joined a plethora of other artists and scientists engaged in coming up with design solutions, and then in circulating money. The process to develop the trends of visual identity on money can be classified into six periods: the end of the 19th century and the beginning of the 20th century, the period between the two world wars, the period after World War II, the period of the Socialist construction, the Dissolution of Yugoslavia, and the period from 2003 until 2018. In the process to develop trends within visual identity, we monitor the chronological sequence of events under the competences and functions of the National Bank. The analysed design solutions for banknotes come from the collection of the National Bank of Serbia, i.e. the Institute for Manufacturing Banknotes and Coins in Topčider. This paper looks into the artistic-technological aspects and trends of developing a visual identity in the service of protecting money from counterfeiting. x In addition, the development of science and technology enabled the implementation of more modern manufacturing procedures and the improvement of the money protection system, while at the same time it made room for greater technical-technological possibilities for counterfeiting. In the second part of the paper, the authors present the potential for implementing optical microscopy and Raman spectroscopy as non-destructive forensic methods for analysing historical and circulating banknotes. The aim of the research was to prove that optical microscopy and Raman spectroscopy have been successfully applied in banknote analysis. Security printing techniques were defined, as well as security elements within the shaping of visual identity, i.e. monetary culture. The experimental part of the paper was conducted on historical banknotes issued between 1893 and 2018." ]

[ "" ]

[ "openAccess" ]

[ "" ]

[ "новац", "Народна Банка", "сигурносне технике штампе", "заштитни елементи", "пигменти", "Раманова спектроскопија", "форензика", "визуелни идентитет на новцу" ]

[ "money", "National Bank", "security printing techniques", "security features", "pigments", "security inks", "Raman spectroscopy", "forensic", "visual identity on money" ]

[ "Формирање и историјски развој визуелног идентитета у служби заштите кованог и папирног новца у Србији (1868-2018)" ]

[ "Formation and historical development of visual identity in service of protection of coins and banknotes in Serbia (1868-2018)" ]

[ "ARR" ]

[ "http://nardus.mpn.gov.rs/bitstream/id/144737/Izvestaj_Komisije_12304.pdf", "http://nardus.mpn.gov.rs/bitstream/id/144736/Disertacija_12304.pdf", "https://fedorabg.bg.ac.rs/fedora/get/o:25798/bdef:Content/download" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_20481" ]

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[ "Korać, Bato" ]

[ "Korać, Bato", "Vučić, Vesna", "Vučić, Vesna", "Glibetić, Marija" ]

[ "2016-01-05T11:46:17", "2020-07-03T08:08:34" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/2061", "http://eteze.bg.ac.rs/application/showtheses?thesesId=50", "https://fedorabg.bg.ac.rs/fedora/get/o:3038/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024528818" ]

[ "Intenzivna fizička aktivnost utiče na metabolizam lipida i masnih kiselina (MK), dovodi do povećane potrošnje kiseonika u mišićima i drugim tkivima što vodi povećanoj produkciji reaktivnih kiseoničnih vrsta i može uticati na funkciju imunog sistema. Uticaj hroničnog intenzivnog vežbanja na parametre oksidativnog stresa i masnokiselinski profil fosfolipida kod aktivnih sportistkinja nije dovoljno ispitivan. Zbog toga je ova studija imala za cilj da ispita MK profil fosfolipida plazme i eritrocita, parametre oksidativnog stresa, aktivnost enzima antioksidativne zaštite, kao i aktivnost monocita kao dela imunog sistema, kod dve grupe aktivnih sportistkinja: vaterpolistkinja (n=15) i fudbalerki (n=19). Kontrolnu grupu (n=14) su činile zdrave devojke istih godina starosti koje se ne bave fizičkom aktivnošću. Uprkos sličnom dijetarnom unosu, MK profil plazme pokazao je znatno veći udeo stearinske, oleinske i mononezasićenih masnih kiselina (MUFA) i značajno niži procenat ukupnih i n-6 polinezasićenih MK (PUFA) kod fudbalerki u odnosu na vaterpolo i kontrolnu grupu. Sa druge strane vaterpolistkinje su imale višu procentualnu zastupljenost palmitoleinske i arahidonske kiseline u odnosu na kontrolnu grupu. MK profil eritrocita pokazao je značajno veći procenat oleinske kiseline i MUFA kod fudbalerki u odnosu na kontrolnu grupu, i smanjeni procenat stearinske kiseline uz viši udeo palmitinske i palmitoleinske kiseline kod vaterpolistkinja u odnosu na druge dve grupe. Obe grupe sportistkinja su imale značajno niži procenat n-6 PUFA i ukupnih PUFA u poređenju sa kontrolnom grupom. Procenjena aktivnost desaturaza i elongaza u eritrocitima takođe je promenjena kod sportistkinja. Analiza parametara oksidativnog stresa pokazala je značajno veći ukupni antioksidativni status u plazmi i veći stepen lipidne peroksidacije u eritrocitima kod sportistkinja u odnosu na kontrolnu grupu. Koncentracija H2O2 u plazmi bila je veća kod sportistkinja u odnosu na neaktivne žene, ali su fudbalerke imale viši nivo H2O2 i u odnosu na vaterpolistkinje. Sa druge strane, koncentracija O2 .- bila je najniža kod vaterpolistkinja a najviša kod fudbalerki, značajno viša i u odnosu na kontrolnu grupu. Koncentracija NO2 - u plazmi bila je značajno viša kod fudbalerki u odnosu na kontrolnu grupu. Aktivnost enzima antioksidativne zaštite u eritrocitima razlikovala se između sportistkinja i kontrolne grupe. Tako je aktivnost katalaze kod sportistkinja bila značajno viša u odnosu na kontrolnu grupu, dok se aktivnost glutationperoksidaze nije razlikovala između ispitivanih grupa. Aktivnost superoksid-dismutaze bila je najviša kod vaterpolistkinja, i nije se značajno razlikovala između fudbalerki i kontrolne grupe. Analiza metaboličke aktivnosti mononuklearnih ćelija periferne krvi pokazala je veću aktivnost mononuklearnih ćelija kod sportistkinja, dok se produkcija O2 .- i NO2- u mononuklearnim ćelijama nije razlikovala između ispitivanih grupa. Naši rezultati su pokazali da dugotrajna intenzivna fizička aktivnost značajno utiče namasnokiselinski profil fosfolipida plazme i eritrocita, parametre oksidativnog stresa, aktivnost enzima antioksidativne zaštite, menja aktivnost mononuklearnih ćelija periferne krvi ali ne utiče na produkciju O2 .- i NO2 - u mononuklearnim ćelijama. Zapažene razlike između grupa vaterpolistkinja i fudbalerki ukazuju da tip sporta igra značajnu ulogu u promenama u metablizmu kod sportistkinja." ]

[ "Intensive physical activity affects metabolism of lipids and fatty acids (FA), increases consumption of oxygen in muscles and other tissues that leads to increased production of reactive oxygen species and can affect immune system. The effect of chronic intensive exercise on parameters of oxidative stress and phospholipids FA composition in elite female athletes has not been studied extensively so far. Thus the aim of this study was to investigate FA profiles in plasma and erythrocytes phospholipids, parameters of oxidative stress, activity of antioxidative enzyme system and activity of monocytes as a part of immune system in two groups of active female athletes: water polo (n=15) and football (n=19) players. The control group was composed of 14 apparently healthy age-matched sedentary women. In spite of similar dietary patterns, plasma FA profile in the football players showed significantly higher proportion of stearic acid, oleic acid and monounsaturated FA (MUFA), and significantly lower proportion of total and n-6 polyunsaturated FA (PUFA) than the water polo and control group. The water polo players had higher percentages of palmitoleic acid and arachidonic acid than the control subjects. Erythrocyte FA profile differed among groups. We found significantly higher proportion of oleic acid and MUFA in the football group than in the controls, and decreased stearic acid and elevated palmitic and palmitoleic acid in the water polo players than in the other two groups. Both groups of athletes had significantly lower percentages of n-6 PUFA and total PUFA compared to the controls. The estimated activities of elongase and desaturases in erythrocytes were also altered in the athletes. Analysis of oxidative stress parameters showed significantly higher plasma total antioxidant status and higher lipid peroxidation in erythrocytes of the athletes than in the controls. Concentration of H2O2 in plasma was higher in the athletes compared with sedentary women, but also higher in football than in the water polo players. On the other hand, level of O2 .- was the lowest in water polo players and the highest in the football group. Concentrations of both O2 .- and NO2 - were significantly higher in the football than in the control group. Catalase activity was significantly higher in the athletes that in sedentary women, while glutation peroxidase activity was similar in all groups. Superoxide-dismutase activity was higher in the water polo group than in the other two groups. Furthermore, metabolic activity of peripheral blood mononuclear cells was higher in the athletes, and production of O2 .- i NO2 - in mononuclear cells did not differ among the studied groups. Our results have shown that long-term, intense physical training significantly affected FA status of plasma and erythrocyte phospholipids, oxidative stress and antioxidant enzyme activity in women. It also changes metabolic activity of mononuclear cells but not the production of O2 .- i NO2 - in mononuclear cells. The observed differences between the water polo and the football players suggest that the type of regular training may contribute to the altered metabolism in athletes. Key words: phospholipids, fatty acids, physical activity, water polo, football, female athletes" ]

[ "info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41030/RS//", "info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41030/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "Fosfolipidi", "masne kiseline", "fizička aktivnost", "vaterpolo", "fudbal", "sportistkinje", "slobodni radikali", "enzimi antioksidativne zaštite", "mononuklearne ćelije" ]

[ "Phospholipids", "fatty acids", "physical activity", "water polo", "football", "female athletes", "free radicals", "antioxidant enzymes", "mononuclear cells" ]

[ "Uticaj hroničnog intenzivnog treninga na parametre oksidativnog stresa i masnokiselinski profil plazme i eritrocita kod sportistkinja", "The effect of chronic intensive training on oxidativ stress parameters and fatty acid profiles of plasma and erythrocytes in female athletes" ]

[ "" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/1909/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1909/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2061" ]

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[ "Marković, Dragana" ]

[ "Vujović, Predrag", "Marković, Dragana", "Vujović, Predrag", "Ilić, Vesna" ]

[ "2017-04-29T21:48:41", "2020-07-03T08:07:33" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/7988", "http://eteze.bg.ac.rs/application/showtheses?thesesId=4893", "https://fedorabg.bg.ac.rs/fedora/get/o:15331/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=48841999" ]

[ "Инфлуенца је инфективна респираторна болест узрокована вирусима инфлуенце. Иако је сама инфекција вирусом инфлуенце ретко била повезивана са различитим орган-специфичним и системским аутоимунским болестима, неуролошке аутоимунске манифестације после примене инфлуенца вакцине су биле документоване током времена. Мултипла склероза (МС) је хронична, у основи аутоимунска неуролошка болест која се најчешће доводи у везу са вакцинацијом. Експериментални аутоимунски енцефаломијелитис (EAE) је најинтензивније изучаван мишји модел МС, а мијелин олигодендроцитним гликопротеином (MOG) индукован EAE је много сличнији МС код људи него друге варијанте ЕАЕ. С обзиром да је расправа о добробити вакцинације болесника са MС још увек отворена циљ наше студије је био давање доприноса у расветљавању улоге вакцине против грипа и њеног ефекта на регулацију ЕАЕ кроз праћење клиничких знакова, титра серумских антитела ELISA тестом, експресије молекула главног хистокомпатибилног комплекса (MHC) I и II класе на пресецима можданог ткива имунохистохемијским методама и ефекат на когнитивне функције (просторно учење и памћење одређивано Морисовим тестом воденог лавиринта; MWM). C57BL/6 мишеви су прво вакцинисани split virion инактивисаном инфлуенца вакцином и три дана касније је индукован ЕАЕ са MOG35-55 пептидом. Укупно добијени резултати представљени у овој дисертацији указују да инфлуенца вакцина није утицала на продукцију анти- MOG антитела и на развој клиничких знакова ЕАЕ. И поред тога што је поремећај на нивоу (ауто)имунског одговора код МС болесника евидентан, имунски одговор на већину уобичајених вирусних и бактеријских инфекција изгледа да је очуван. Резултати ове студије су показали да је титар анти- инфлуенца антитела код здравих вакцинисаних мишева и код оних код којих је након вакцинације индукован ЕАЕ, четири недеље након вакцинације, био идентичан, што указује на очуваност хуморалног имунског одговора и код мишева код којих је индукован ЕАЕ..." ]

[ "Influenza is a contagious respiratory illness caused by influenza viruses. Influenza infection per se has seldom been associated with various organ-specific and systemic autoimmune diseases but neurological autoimmune phenomena have been reported following influenza vaccine. Multiple sclerosis (MS) is the most common chronic neurological disease of an autoimmune nature that has been viewed in conjunction with vaccination. Experimental autoimmune encephalomyelitis (EAE) is the most extensively studied mouse model of MS and myelin oligodendrocyte glycoprotein (MOG) induced EAE more closely resembles MS than other EAE variants. Since the debate about vaccine safety in patients with MS is still wide open, the objective of our study was to shed more light on and supply additional information about the influenza vaccination effects on regulation of EAE through monitoring of clinical signs, serum antibody titers by using ELISA, expression of MHC (Major Histocompatibility Complex) class I and II molecules in brain tissue by immunohistochemical detection, and its effect on cognitive functions (spatial learning and memory measured by Morris Water Maze test). C57BL/6 mice were first vaccinated with influenza split virion inactivated vaccine and three days later EAE was induced with MOG35-55 peptide. The overall data presented in this study indicate that influenza vaccine had no effect on the production of anti-MOG autoantibodies and the development of clinical signs. Although the unbalanced immune system is evident in patients with MS, immune defense against common viral and bacterial infections appears to be preserved. In this study anti-influenza antibody titers in healthy vaccinated mice and in MOG induced EAE-vaccinated mice, four weeks after vaccination with influenza vaccine have indicated preserved long-lasting antibody response in MOG induced EAE mice. High expression of MHC II and mild-to-low expression of MHC I was detected in mice with EAE..." ]

[ "" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "Вакцина против грипа", "Pertussis вакцина", "Мијелин олигодендроцитни гликопротеин", "Експериментални аутоимунски енцефаломијелитис", "Хороидни плексус", "Хипокампус", "MHC молекули I и II класе", "CD4 молекул", "Просторно учење", "Морисов тест воденог лавиринта" ]

[ "Influenza vaccine", "Choroid plexus", "CD4 molecules", "Hyppocampus", "MHC molecules class I and II", "Pertussis vaccine", "Myelin oligodendrocyte glycoprotein", "Experimental autoimmune encephalomyelitis", "Spatial learning – Morris water maze test" ]

[ "Улога инактивисане вакцине против грипа у регулацији аутоимунских процеса у експерименталном аутоимунском енцефаломијелитису" ]

[ "Role of inactivated influenza vaccine in regulation of autoimmune processes in experimental autoimmune encephalomyelitis" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/1631/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1632/IzvestajKomisije8387.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1631/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1632/IzvestajKomisije8387.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_7988" ]

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[ "Kostić, Tatjana" ]

[ "Kostić, Tatjana", "Matić, Gordana", "Matić, Gordana", "Andrić, Silvana" ]

[ "2017-06-17T08:53:24", "2020-07-03T08:08:18" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/8303", "http://eteze.bg.ac.rs/application/showtheses?thesesId=5055", "https://fedorabg.bg.ac.rs/fedora/get/o:15785/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025154738" ]

[ "Biološki časovnik organizuje metabolizam i fiziološke procese u cirkadijalne ritmove. Na nivou ćelije, on se sastoji od grupe gena koji preko negativnih povratnih sprega održavaju ritam sopstvene transkripcije, ali regulišu i ritmičnost transkripcije mnogih drugih gena. Iako je poznato da su određeni geni časovnika neophodni za sintezu testosterona i fertilnost mužjaka, još uvek nema preciznih podataka o cirkadijalnoj fiziologiji testosteron produkujućih Lajdigovih ćelija. Ova studija je dizajnirana da definiše (1) cirkadijalni obrazac endokrine funkcije Lajdigovih ćelija uključujući i eksprimiranje gena perifernog časovnika i (2) upletenost LH-cAMP signalizacije u sinhronizaciju ritma Lajdigovih ćelija korišćenjem in vivo modela poremećene cAMP homeostaze (hipogonadotropni hipogonadizam, starački hipogonadizam, pinealektomija) i in vitro stimulacije Lajdigovih ćellija. Rezultati su pokazali cirkadijaln ritam funkcije Lajdigovih ćelija koji se ogleda u vremenski koordinisanoj oscilatornoj produkciji testosterona i intracelularnog cAMP, cirkadijalnom eksprimiranju regulatora (Nur77 i Arr19), steroidogenih elementa (Star/StAR, Cyp11a1 i Cyp17a1), kao i elementa časovnika (Bmal1/BMAL1, Per1/2/3, Cry1/2, Rev-erba/b/REV-ERBA, Rorb, Dec1/2, Dbp i E4bp4). Ritam transkripcije osnovnih gena časovnika kao i ključnog elementa steroidogeneze (Star) se održava i u primarnoj kulturi ovih ćelija. Redukcija cAMP detektovana u Lajdigovim ćelijama pacova sa hipogonadotropnim hipogonadizmom stimuliše transkripciju većeg broja gena časovnika: Per2, Rorb, Rev-erbb, Dec1/2, E4bp4, Ck1e/d, i inhibiše Npas2. Sa druge strane, in vitro stimulacija cAMP-signalizacije povećava transkripciju Per1, Dec1/2, Rorb, Npas2 i E4bp4, i smanjuje transkripciju Rev-erba. Starenje, dovodi do opadanja robusnosti cirkadijalne funkcije Lajdigovih ćelija koja se ogleda u smanjenju oscilacija intracelularnog cAMP, smanjenja amplitude eksprimiranja najvažnijih gena časovnika (Bmal1/BMAL1, Per1/2, Rev-erba/REV-ERBA), gena uključenih u metabolizam holesterola (Lipe, Soat2, Scarb1) i steroidogenih gena, (Star/StAR, Cyp11a1, Cyp17a1, Hsd3b1/2/HSD3B, Hsd17b4)..." ]

[ "Biological clock organizes metabolic and physiological processes in circadian rhythms. At cell level, it consists of group of genes that regulate its own transcription by negative feedback loop, also regulating transcription rhythmicity of other genes. Although, it is known that some clock genes are necessary for testosterone synthesis and male fertility, there is no precise data about circadian physiology of testosterone-producing Leydig cells. This thesis was design to define (1) circadian pattern of endocrine function of Leydig cells, including expression of clock genes, and (2) involvement of LH-cAMP signaling in synchronization of Leydig cells rhythm using in vivo model of disturbed cAMP homeostasis (hypogonadotropic hypogonadism, hypogonadism in aging, pinealectomy) and in vitro Leydig cell stimulation. Results confirmed circadian rhythmicity of Leydig cell function represented by temporal coordination of cyclic testosterone production and intracellular cAMP, circadian expression of regulators (Nur77, Arr19), steroidogenic (Star/StAR, Cyp11a1 i Cyp17a1) and clock elements (Bmal1/BMAL1, Per1/2/3, Cry1/2, Rev-erba/b/REV-ERBA, Rorb, Dec1/2, Dbp, E4bp4). Rhythm in transcription of core clock genes as well as key steroidogenic element (Star) was preserved in primary Leydig cell culture. Reduction in cAMP, detected in Leydig cells from hypogonadotropic hypogonadal rats, stimulated transcription of some clock genes: Per2, Rorb, Rev-erbb, Dec1/2, E4bp4, Ck1e/d, but inhibited Npas2. On the other hand, in vitro stimulation of cAMP signaling increased transcription of Per1, Dec1/2, Rorb, Npas2 and E4bp4, and reduced transcription of Rev-erba. Aging dulled robustness of circadian function of Leydig cells, represented by decline in intracellular cAMP oscillations and amplitude of expression of core clock genes (Bmal1/BMAL1, Per1/2, Rev-erba/REV-ERBA), genes involved in cholesterol metabolism (Lipe, Soat2, Scarb1) and steroidogenic genes (Star/StAR, Cyp11a1, Cyp17a1, Hsd3b1/2/HSD3B, Hsd17b4). Abolishment of melatonin, a main cue that spread information of light regime via cAMP signaling, stimulated expression of clock (Bmal1/BMAL1, Per1/2) and steroidogenic (Star/StAR, Hsd3b/HSD3B) elements..." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173057/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]

[ "Lajdigove ćelije", "cirkadijalni časovnik", "steroidogeneza", "testosteron", "cAMP", "cGMP", "starenje", "hipogonadizam", "melatonin", "pacov" ]

[ "Leydig cells", "circadian clock", "steroidogenesis", "testosterone", "cAMP", "cGMP", "aging", "hypogonadism", "melatonin", "rat" ]

[ "Karakterizacija i putevi sinhronizacije perifernog biološkog časovnika i steroidogeneze u Lajdigovim ćelijama pacova" ]

[ "Characterization and pathways of synhronization of peripheral biological clock and steroidogenesis in rat Leydig cells" ]

[ "BY-NC-SA" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/1856/IzvestajKomisije10978.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1855/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1855/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1856/IzvestajKomisije10978.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_8303" ]

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[ "Uskoković, Aleksandra" ]

[ "Vidaković, Melita", "Savić-Pavićević, Dušanka", "Uskoković, Aleksandra", "Vidaković, Melita" ]

[ "2020-02-07T10:29:24", "2020-07-03T08:08:06" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/11828", "http://eteze.bg.ac.rs/application/showtheses?thesesId=7183", "https://fedorabg.bg.ac.rs/fedora/get/o:20881/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51818255" ]

[ "Važan aspekt u rasvetljavanju procesa demetilacije DNK predstavlja identifikacija faktora koji regulušu aktivnost TET proteina. Cilj ove doktorske disertacije bio je da se ispita funkcionalna veza između TET-posredovane oksidacije 5mC i PARP-zavisne PARilacije u procesu demetilacije DNK, na globalnom i lokalnom nivou. Rezultati ovog istraživanja ukazuju da PARP-1 interaguje sa TET1 i TET2. Pokazano je i da oba proteina podležu in vitro PARilaciji. PARP-1-zavisna PARilacija TET1 proteina negativno utiče na kinetiku aktivnosti TET1 in vitro. Rezultati in cellulo eksperimenata pokazali su da u odsustvu PARP-1 dolazi do porasta ekspresije Tet1 i Tet2, a da prilikom inhibicije PARilacije i u odsustvu PARP-1 dolazi do pada globalnog nivoa metilacije i porasta hidroksimetilacije DNK. Za analizu lokalnih efekata TET-PARP interakcije izabran je gen za hemokin CXCL12 koji učestvuje u brojnim fiziološkim i patološkim procesima pa je ispitivanje regulacije njegove ekspresije važno za buduće terapijske pristupe. Pokazano je da u odsustvu PARP-1 dolazi do povećane ekspresije kao i do pada u metilaciji Cxcl12. Nakon tretmana aktivatorom/inhibitorom TET aktivnosti, u odsustvu PARP-1, ekpresija Cxcl12 je povećana/smanjena. Prisustvo TET1 i TET2 detektovano je na promotoru Cxcl12 uz povećano regrutovanje TET2 u odsustvu PARP-1. U ovoj disertaciji pokazan je inhibitorni uticaj PARP-1 i PARilacije na aktivnost TET enzima u procesu demetilacije DNK na globalnom nivou, a funkcionalnost lokalnih efekata povezanosti TET i PARP enzima u procesu (de)metilacije ustanovljena je na primeru Cxcl12. Demetilacija DNK nalazi se u osnovi brojnih fizioloških i patoloških stanja, zato rasvetljavanje mehanizama regulacije ovog procesa predstavlja važan korak u potencijalnoj primeni stečenih saznanja u medicini." ]

[ "elucidating the process of DNA demethylation. The aim of this doctoral dissertation was to investigate the functional relationship between TET-mediated oxidation of 5mC and PARP-dependent PARylation in the process of DNA demethylation at both global and local levels. This thesis shows that PARP-1 interacts with TET1 and TET2, and that both TET proteins can undergo in vitro PARylation. PARP-1-dependent PARylation of TET1 negatively affected the kinetics of TET1 activity in vitro. The results of in cellulo experiments revealed that the expression of Tet1 and Tet2 increased in the absence of PARP-1, whereas when PARylation was inhibited or in the absence of PARP-1, there was a decrease in the global level of DNA methylation and an increase in DNA hydroxymethylation. The Cxcl12 gene was selected for the analysis of the local effects of TET-PARP interaction since chemokine CXCL12 participates in numerous physiological and pathological processes, and exploring the regulation of expression of this gene is important for potential clinical intervention. Increased expression and decreased methylation of Cxcl12 were observed in the absence of PARP-1. After treatment with an activator or inhibitor of TET activity in the absence of PARP-1, Cxcl12 expression was respectively increased or decreased. The presence of TET1 and TET2 was detected on the Cxcl12 promoter, with enhanced recruitment of TET2 in the absence of PARP-1. This dissertation describes the inhibitory effects of PARP-1 and PARylation on the activity of TET enzymes in the process of DNA demethylation at the global level, and the local effects of TET-PARP interplay on DNA (de)methylation of Cxcl12 gene. DNA demethylation is at the root of numerous physiological and pathological conditions, and elucidating the mechanisms that regulate this process is an important step in the potential application of acquired knowledge in medicine." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173020/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]

[ "TET1", "TET2", "PARP-1", "PAR polimeri", "PARilacija", "metilacija DNK", "demetilacija DNK", "Cxcl12 gen" ]

[ "TET1", "TET2", "PARP-1", "PAR polymers", "PARylation", "DNA methylation", "DNA demethylation", "Cxcl12 gene" ]

[ "Funkcionalna analiza interakcija TET-posredovane oksidacije 5-metilcitozina i PARP-zavisne ADP-ribozilacije u procesu demetilacije DNK" ]

[ "Functional analysis of interactions between TET-mediated oxidation 5-methylcytosine and PARP-dependent ADP-ribosylation in the process of DNA demethylation" ]

[ "BY-NC-SA" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/1800/IzvestajKomisije21854.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1799/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1800/IzvestajKomisije21854.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1799/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_11828" ]

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[ "Drakulić, Danijela" ]

[ "Stevanović, Milena", "Brajušković, Goran", "Drakulić, Danijela", "Stevanović, Milena" ]

[ "2020-01-27T12:20:32", "2020-07-03T08:07:57" ]

[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=7077", "https://nardus.mpn.gov.rs/handle/123456789/11740", "https://fedorabg.bg.ac.rs/fedora/get/o:20712/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025220274" ]

[ "Glioblastom, glioma tumor gradusa IV, je najčešći maligni tumor mozga kod odraslih i jedan od najsmrtonosnijih tipova tumora. I pored agresivne terapije koja obuhvata hirurško uklanjanje tumora, radio- i hemio-terapiju, prosečno preživljavanje bolesnika sa ovim tipom tumora je oko 15 meseci. Glioblastom (GBM), pored tumorskih ćelija, sadrži i populaciju samo-obnavljajućih tumor-inicirajućih matičnih ćelija (matične ćelije glioblastoma) koje se smatraju odgovornim za nastanak, progresiju, metastaziranje i rezistenciju na terapiju. Geni SOXB1 podgrupe (SOX1, SOX2 i SOX3) kodiraju regulatorne proteine koji imaju značajne uloge u mnogim procesima u toku razvića, kao što su održavanje pluripotentnosti matičnih ćelija i održavanje populacije neuralnih progenitora u pluripotentnom i proliferišućem stanju. Ovi geni imaju i značajne funkcije u procesu karcinogeneze. Ekspresija gena ove podgrupe detektovana je u GBM. Funkcija gena SOX2 je dobro proučena kod ovog tipa tumora; pokazano je da ovaj gen promoviše maligni potencijal ćelija GBM i neophodan je za održavanje tumorogenog potencijala matičnih ćelija glioblastoma. Za razliku od gena SOX2, uloga gena SOX1 i SOX3 u ćelijama GBM još uvek nije dovoljno istražena. Stoga, u okviru ove doktorske disertacije analizirana je uloga ovih gena u ćelijama glioblastoma. Dobijeni rezultati pokazuju da ćelijske linije GBM eksprimiraju gen SOX1. Pored toga, u uslovima utišane ekspresije proteina SOX1 detektovano je smanjenje proliferativnog kapaciteta, vijabilnosti i migratornog potencijala U251 ćelija GBM, kao i povećanje broja ovih ćelija u senescenciji. Nakon dediferencijacije ćelijskih linija GBM detektovano je povećanje ekspresije gena SOX1 u poređenju sa ekspresijom uočenom u njihovim parentalnim ćelijama. Nivo ekspresije gena SOX1 povećan je u kulturama matičnih ćelija glioblastoma u poređenju sa ekspresijom ovog gena u imortalizovanim U87 i U251 ćelijama; pri diferencijaciji ovih kultura uočeno je smanjenje ekspresije gena SOX1. Utišavanje ekspresije gena SOX1 u GNS166 kulturi matičnih ćelija GBM dovodi do smanjenja proliferativnog kapaciteta i vijabilnosti ovih ćelija..." ]

[ "one of deadliest cancers. Despite aggressive treatment, including surgical resection, chemotherapy and radiation, the median survival of patients with glioblastoma is 15 months. A growing body of evidence indicates that GBM contains a population of selfrenewing tumor-initiating cells (glioblastoma stem cells - GSCs) that drive tumor initiation, propagation, metastasis and therapy resistance. SOXB1 genes (SOX1, SOX2 and SOX3) encode transcription regulators with important roles in embryonic development and carcinogenesis. Literature date revealed that SOXB1 genes are expressed in GMB tumor samples. The role of one member of this group, SOX2 gene, is well documented in GBM. It was shown that SOX2 gene promotes malignant potential of GBM cells and it is mandatory for maintenance of tumorogenicity of GSCs. Since the function of SOX1 and SOX3 genes in GBM still remains to be established, the aim of this thesis was to analyze the role of these genes in GBM. Obtained results demonstrated that all analyzed GBM cell lines express SOX1. Downregulation of this gene expression decreases proliferation, viability and migration, and induces senescence of U251 cells. Furthermore, dedifferentiation of GBM cells is accompanied by increase of SOX1 level compared to that in parental cells. Expression of this gene was significantly increased in patient-derived GSC cultures compared to that in U87 and U251 cells; downregulation of SOX1 gene expression was seen upon differentiation of GSCs. In addition, knock-down of this gene expression reduced proliferation and viability of GSCs. Results obtained in this thesis reveal SOX3 expression in GBM cell lines and tumor tissue; the expression of this gene was elevated in the most of analyzed GBM samples compared to expression levels detected in non-tumoral brain tissues. A high SOX3 expression was not associated with the overall survival of GBM patients. Ectopic overexpression of this gene increased proliferation, viability, migration and invasion of GBM cells..." ]

[ "" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "glioblastom", "SOX1", "SOX3", "matične ćelije glioblastoma", "maligni fenotip", "diferencijaciona terapija", "retinoična kiselina" ]

[ "glioblastoma", "SOX1", "SOX3", "glioblastoma stem cells", "malignant phenotype", "differentiation therapy", "retinoic acid" ]

[ "Analiza uloge gena SOX1 i SOX3 u promovisanju malignog fenotipa ćelija glioblastoma" ]

[ "Analysis of the role of SOX1 and SOX3 genes in promotion of malignant phenotype of glioblastoma cells" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/1747/IzvestajKomisije21589.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1746/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1746/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1747/IzvestajKomisije21589.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_11740" ]

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[ "Stojković, Biljana" ]

[ "Lazarević, Jelica", "Šešlija- Jovanović, Darka", "Petrović, Anđeljko" ]

[ "2017-06-17T08:53:25", "2020-07-03T08:08:19" ]

[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=5056", "https://nardus.mpn.gov.rs/handle/123456789/8304", "https://fedorabg.bg.ac.rs/fedora/get/o:15786/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025151410" ]

[ "Ključna pretpostavka evolucione teorije starenja jeste da opadanje uzrasno-specifičnog preživljavanja organizama predstavlja rezultat smanjenja intenziteta prirodne selekcije nakon početka reprodukcije. U osnovi evolucije starenja nalaze se dva populaciono-genetička mehanizma: akumulacija mutacija (AM) i antagonistička plejotropija (AP). U skladu sa pretpostavkama evolucione teorije starenja, kod eksperimentalnih populacija pasuljevog žiška (Acanthoscelides obtectus) selektovanih više od 170 generacija za ranu (E režim) ili kasnu (L režim) reprodukciju, uočena je divergencija u dužini života i drugim preadultnim i adultnim osobinama životne istorije. Evolucija veće dužine života u L režimu u saglasnosti je sa pretpostavkama AP modela, dok u evoluciji kraćeg života E žižaka važnu ulogu ima akumulacija mutacija. Tretiranjem E i L žižaka pesticidima (parakvat i tebufenpirad) koji generišu slobodne radikale, potvrđena je pozitivna korelacija između dužine života i otpornosti na oksidativni stres. Budući da mitohondrija predstavlja glavno mesto sinteze energije i slobodnih radikala, u ovoj tezi je istraženo da li je uzrasno-specifična selekcija oblikovala varijabilnost mtDNK, nDNK i njihovih epistatičkih interakcija. Genotipizacijom mitohondrijskog COI gena i mikrosatelitskih lokusa pokazana je efikasnost uzrasno-specifične selekcije u međupopulacionoj divergenciji mitohondrijskog i jedarnog genskog pula kod pasuljevog žiška. Odvijanje procesa mito-jedarne koevolucije analizirano je korišćenjem mito-jedarnih linija u kojima su E i L mitohondrijski genomi kombinovani sa E i L jedarnim genomima. Pokazano je da su linije sa narušenim mito-jedarnim interakcijama imale niže aktivnosti mitohondrijskih kompleksa elektron transportnog lanca i lošiju preadultnu performansu." ]

[ "According to the evolutionary theory of ageing, age-specific decline in survival is the result of decreasing intensity of natural selection after the onset of reproduction. The evolution of ageing may be explained by two different, but not mutually exclusive, genetic mechanisms: antagonistic pleiotropy (AP) and mutation accumulation (MA). In accordance with evolutionary theory of ageing, two sets of the seed beetle (Acanthoscelides obtectus) experimental lines selected for more than 170 generations for early (E regime) or late (L regime) reproduction, show divergence in longevity and ageing patterns, as well as in other preadult and adult life history traits. Evolution of long life in L regime is predominantly based on antagonistic pleiotropy gene effects, while mutation accumulation plays important role in evolution of short life in E regime. By exposure of E and L beetles to two pesticides (paraquat and tebufenpyrad) that generate free radicals, it was confirmed that extended longevity in L beetles (especially in females) was associated with higher oxidative stress resistance. Additionally, since mitochondria are main site of free radical and energy production, effects of age-specific laboratory selection on mtDNA, nDNA and their epistatic interaction were assessed. Genotyping of mitochondrial COI gene and nuclear microsatellite loci showed that selection in E and L regimes led to divergent evolution of mtDNA and nDNA gene pools. Mitonuclear coevolution was investigated using mitonuclear introgression lines in which E and L mitochondrial genomes were expressed in both E and L nuclear background. It was found that lines with disrupted mitonuclear interactions had lower activity of the mitochondrial electron transport chain complexes and lower preadult performance." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173007/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "starenje", "mitohondrije", "oksidativni stres", "mito-jedarne interakcije", "elektron transportni lanac", "osobine životne istorije", "laboratorijska evolucija", "Acanthoscelides obtectus" ]

[ "ageing", "mitochondria", "oxidative stress", "mitonuclear interactions", "electron transport chain", "life history traits", "laboratory evolution", "Acanthoscelides obtectus" ]

[ "Evolucija starenja kod laboratorijskih populacija Acanthoscelides obtectus: uloga mitohondrija i oksidativnog stresa" ]

[ "Evolution in laboratory populations of Acanthoscelides obtectus: role of mitochondria and oxidative stress" ]

[ "BY-NC-ND" ]

[ "http://nardus.mpn.gov.rs/bitstream/id/1862/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1863/IzvestajKomisije10979.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1863/IzvestajKomisije10979.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1862/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_8304" ]

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[ "Ivanović, Ana" ]

[ "Ivanović, Ana", "Cvijanović, Milena", "Cvijanović, Milena", "Stojković, Biljana" ]

[ "2020-02-07T10:29:22", "2020-07-03T08:08:04" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/11824", "http://eteze.bg.ac.rs/application/showtheses?thesesId=7178", "https://fedorabg.bg.ac.rs/fedora/get/o:20869/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025238450" ]

[ "Hibridizacija može imati različite evolucione i ekološke ishode. Veliki mrmoljci (Triturus spp.) formiraju hibridne zone u kojima učestvuju vrste različitog stepena filogenetske srodnosti i predstavljaju pogodan model sistem za ispitivanje procesa i mehanizama hibridizacije i njenih ishoda. Vrste T. ivanbureschi i T. macedoncius hibridizuju u centralnoj i istočnoj Srbiji formirajući specifičnu zonu u kojoj dolazi do zamene vrsta i asimetrične introgresije mitohondrijske DNK uz postojanje vijabilnih hibridnih populacija. Relativno sličan scenario sreće se u zapadnoj Francuskoj gde T. cristatus i T. marmoratus hibridizuju i produkuju sterilne hibride. Ciljevi ove doktorske disertacije su određivanje parametara koji su doprineli formiranju i održavanju hibridne zone, kao i procena uticaja hibridizacije na vijabilitet, morfološku varijabilnost (sa posebnim osvrtom na složeni životni ciklus i metamorfozu) i stabilnost razvića hibrida i roditeljskih vrsta. Podaci su prikupljani tokom tri godine intra- i interspecijskog eksperimentalnog ukrštanja, kao i analizom materijala iz prirodnih populacija. Praćeni su parametri rane životne istorije, ontogenija i disparitet oblika tela i uticaj hibridizacije na stabilnost razvića. Morfološka varijabilnost i stabilnost razvića procenjeni su metodama geometrijske morfometrije. Triturus macedonicus odlikuje se većim reproduktivnim potencijalom u odnosu na T. ivanbureschi, što je moglo da omogući početak širenja areala. Hibridizacija smanjuje vijabilitet jaja, ali ne i embriona. Hibridi se morfološki razlikuju od obe roditeljske vrste i uglavnom su intermedijarni. Larveno razviće je kanalisano, dok su stupnjevi kada jedinke dolaze u kontakt sa novom sredinom (izvaljivanje larvi i metamorfoza) izuzetno varijabilni. Hibridizacija ne utiče na stabilnost razvića velikih mrmoljaka." ]

[ "Hybridization can have various evolutionary and ecological outcomes. Large-bodied newts (Triturus spp.) hybridize mutually regardless of the degree of genetic similarity. This characteristic makes them a good model system to explore hybridization, as well as hybridization outcomes. Triturus ivanbureschi and T. macedonicus have specific species displacement scenario which includes hybridization in Serbia, wide zone of asymmetric mtDNA introgression and viable hybrid populations. Relatively similar species displacement scenario is found in France, where T. cristatus and T. marmoratus hybridize, but their hybrids are sterile. The main aims of this doctoral dissertation are to establish parameters which enabled formation and maintenance of hybrid and introgressive zone and to estimate effects of hybridization on viability, morphological variation (with special emphasis on complex, biphasic life cycle and metamorphosis) and developmental stability. The data was collected during a three-year hybridization experiment, as well as from natural populations. The early life history parameters, ontogeny and disparity of body shape, and the effects of hybridisation on developmental stability were recorded. Morphological variability and developmental stability were analysed by geometric morphometrics. Triturus macedonicus have higher reproductive output compared to T. ivanbureschi which could be an advantage at the initial species contact. Hybridization reduces egg viability, but not the viability of embryos. Hybrids are morphologically divergent from parental species, with mostly intermediate position relative to parental species. Larval development is highly canalized, while the ontogenetic stages when newts confront new environment (hatching and metamorphosis) are highly variable. Hybridization does not affect developmental stability." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173043/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "hibridizacija", "vijabilnost", "morfološka varijabilnost", "varijansa", "morfološki disparitet", "ontogenetske trajektorije", "metamorfoza", "stabilnost razvića", "fluktuirajuća asimetrija", "Triturus spp." ]

[ "hybridisation", "viability", "morphological variability", "variance", "morphological disparity", "ontogenetic trajectories", "metamorphosis", "developmental stability", "fluctuating asymmetry", "Triturus spp." ]

[ "Hibridizacija, morfološka varijabilnost i stabilnost razvića velikih mrmoljaka (Triturus spp.)" ]

[ "Hybridization, morphological variability and developmental stability of large-bodied newts (Triturus spp.)" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/1786/IzvestajKomisije21834.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1785/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1785/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1786/IzvestajKomisije21834.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_11824" ]

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[ "Ćurčić, Srećko" ]

[ "Stanisavljević, Ljubiša", "Kereši, Tatjana", "Pešić, Vladimir", "Ćurčić, Srećko" ]

[ "2016-01-05T11:45:52", "2020-07-03T08:08:41" ]

[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=63", "https://nardus.mpn.gov.rs/handle/123456789/2045", "https://fedorabg.bg.ac.rs/fedora/get/o:3424/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024543410" ]

[ "U studiji je dat prikaz jedanaestogodišnjih istraţivanja insekata iz reda Lepidoptera na Fruškoj gori. Registrovane su 934 vrste leptira i moljaca. Za 382 vrste Lepidoptera ili 40,89% od ukupnog broja vrsta izvršena je taksonomska verifikacija analizom hitinskih armatura genitalnih aparata. U periodu od 2001. do 2011. godine sakupljani su leptiri i moljci uglavnom uz pomoć svetlosne klopke (ţivine sijalice TEŢ WTF od 250 W, “Philips Ml“ od 100, 160, 250 i 400 W i petromaks lampe od 400 W), iza koje je postavljeno belo pamuĉno platno. 934 zabeleţene vrste su svrstane u 22 superfamilije, 47 familija i 564 rodova. Broj od 934 vrste Lepidoptera predstavlja u odnosu na evropsku faunu leptira (8478 vrsta) 11,01%. Sve vrste leptira i moljaca Fruške gore svrstane su u 7 zoogeografskih kategorija. PreovlaĊuju evroazijske vrste (449 vrsta ili 48,07% od ukupnog broja vrsta). Evropskih vrsta je 236 (25,27% od ukupnog broja vrsta). Mediteransko-azijski elementi su zastupljeni sa 162 vrste ili 17,34% od ukupnog broja vrsta. Holarktiĉki elementi zastupljeni su sa 44 vrste ili sa 4,71% od ukupnog broja vrsta, a palearktiĉki sa 25 vrsta ili 2,68% od ukupnog broja vrsta. Paleotropsko-suptropski elementi zastupljeni su sa 10 vrsta ili sa 1,07% od ukupnog broja vrsta. Kosmopolitskih vrsta je 8 vrsta ili 0,86% od ukupnog broja vrsta. Sve evidentirane vrste u ovoj studiji grupisane su u pet skupova: mali moljci, veliki moljci, dnevni leptiri, zemljomerke i sovice. Ukupno 287 vrsta malih moljaca svrstano je u 16 superfamilija i 31 familiju. Više od 5% vrsta malih moljaca Evrope (5,16%) je zastupljeno na Fruškoj gori. Veliki moljci (Lasiocampidae, Saturniidae, Sphingidae, Drepanidae, Notodontidae, Lymantriidae i Arctiidae) su zastupljeni na Fruškoj gori sa 77 vrsta . Više od ĉetvrtine vrsta velikih moljaca Evrope (27,5%) je zastupljeno na Fruškoj gori. Dnevni leptiri (Hesperiidae, Papilionidae, Pieridae, Lycaenidae i Nymphalidae) su zastupljeni na Fruškoj gori sa 115 vrsta. Više od ĉetvrtine vrsta dnevnih leptira Evrope (26,13%) je zastupljeno na Fruškoj gori. Zemljomerke su zastupljene na Fruškoj gori sa 187 vrsta. Oko petine vrsta zemljomerki Evrope (20,43%) je zastupljeno na Fruškoj gori. Sovice (Noctuidae, Pantheidae, Nolidae) su zastupljene na Fruškoj gori sa 268 vrsta. Više od petine vrsta sovica Evrope (20,87%) je zastupljeno na Fruškoj gori. Najbrojnija je grupa malih moljaca sa 287 zabeleţenih vrsta ili sa 30,73% od ukupnog broja utvrĊenih vrsta. Zatim sledi grupa sovica sa 268 zabeleţenih vrsta ili 28,69% od ukupnog broja utvrĊenih vrsta. Na trećem mestu po zastupljenosti nalazi se grupa zemljomerki sa 187 zabeleţenih vrsta ili sa 20,02% od ukupnog broja utvrĊenih vrsta. Slede dnevni leptiri (sa 115 vrsta ili 12,31% od ukupnog broja vrsta) i veliki moljci (sa 77 vrsta ili 8,24% od ukupnog broja vrsta). Na Fruškoj gori je zabeleţeno 129 vrsta Lepidoptera (13,80% od ukupnog broja vrsta) koje mogu naneti štetu lišćarskom i ĉetinarskom drveću. Istraţivanjima na Fruškoj gori identifikovana je 61 vrsta Lepidoptera iz grupe migratornih vrsta, tj. selica. Sezonskih selica prvog reda ima 7 vrsta, sezonskih selica drugog reda 1 vrsta, selica iseljenika (lokalnih selaca prvog i drugog reda) 36 vrsta, selica raseljenika (povremenih selaca) 10 vrsta, selica raseljenika (vrsta koje proširuju svoj areal) 2 vrste, dok mogućih selica ima 5 vrsta. U odnosu na zabeleţeni broj od 934 vrste leptira, procenjuje se da je poznato svega oko jedne trećine vrsta koje bi se mogle otkriti na Fruškoj gori. PronaĊene su 133 nove vrste iz reda Lepidoptera za faunu Srbije. Prvi put u Srbiji, ali i u zemljama u sastavu bivše SFRJ je kao prilog poznavanju faune Lepidoptera u ovoj studiji dat detaljan prikaz hitinskih armatura genitalnih aparata sa opredeljujućim taksonomskim karakteristikama za 250 vrsta (26,76% od ukupnog broja vrsta) uglavnom iz grupe malih moljaca i za nekoliko izdvojenih taksona ostalih grupa. Dat je uporedni prikaz brojnosti vrsta po familijama i superfamilijama za Frušku goru, Timoĉku krajinu, Evropu, Rumuniju, rumunski Banat i MaĊarsku. Lepidoptera su veoma slabo istraţeni na pojedinim podruĉjima Fruške gore. Ovo se prvenstveno odnosi na podruĉje juţno od Dunava oko Neština, Suseka, Banoštora i Ĉerevića, deo od Sremskih Karlovaca do Petrovaradina i Ĉortanovaca, od Krušedola i Neradina do Iriga sa juţne strane Fruške gore i od Vrdnika do Leţimira sa jugozapadne strane (izuzimajući podruĉje oko grgurevaĉkog lovaĉkog doma, selo Grgurevci). Neophodno je da se faunistiĉka i ekološka istraţivanja nastave. Veliki broj lokaliteta je nedovoljno istraţen, na pojedinim mikrolokalitetima istraţivanja nisu ni otpoĉela, a potencijalno su na njima se mogu naći zanimljivi nalazi." ]

[ "This thesis presents results of an eleven-year research on insects belonging from order Lepidoptera on Mt. Fruška Gora. Total of 934 species were recorded. For 382 species Lepidoptera, or 40.89% of the total number of species, a taxonomic verification has been performed by the analysis of chitinous armatures of genital apparatus. In the 2001-2011 period, the specimens were collected mostly by light trap (mercury bulb TEŢ WTF of 250W, “Philips Ml“ of 100, 160, 250 and 400W, and Petromax lamp of 400W), with a white cotton sheet propped in behind. 934 recorded species of Lepidoptera belong to 22 superfamilies, 47 families and 564 genera. The number of 934 species represents 11,01% of European fauna of Lepidoptera (8,478 species). The Lepidoptera species from Mt. Fruška Gora are classified into seven zoogeographical categories. Eurasian species are prevailing (449 species or 48.07% of the total number of species). We recorded 236 European species (25.27% of the total number of species) and 162 Mediterranean-Asian species (17.34% of the total number of species). Holarctic elements are represented by 44 species (4.71% of the total number of species), Palearctic elements by 25 species (2.68% of the total number of species), while Paleotropical-subtropical elements are represented by 10 species (1.07% of the total number of species). Finally, cosmopolitan elements are represented by eight species (0.86% of the total number of species). Within the research, all registered species were classified into five groups: Microlepidoptera (micromoths), macromoths, butterflies, geometrid moths and owlet moths. 287 species of micromoths are classified into 16 superfamilies and 31 families. More than 5% of European species of micromoths (5.16%) are present on Mt. Fruška Gora. Macromoths (Lasiocampidae, Saturniidae, Sphingidae, Drepanidae, Notodontidae, Lymantriidae and Arctiidae) are represented on Mt. Fruška Gora with 77 species. More than one quarter of European species of macromoths (27.5%) are recorded on Mt. Fruška Gora. Butterflies (Hesperiidae, Papilionidae, Pieridae, Lycaenidae and Nymphalidae) are represented on Mt. Fruška Gora with 115 species, which is more than a quarter of the butterfly species of Europe (26.13%). Geometrid moths are represented by 187 species on Mt. Fruška Gora, which is about one fifth of European species of Geometridae (20.43%). Owlet moths (Noctuidae, Pantheidae and Nolidae) are represented on Mt. Fruška Gora by 268 species, which is more than a fifth of the European owlet moth species (20.87%). The micromoths are the most numerous group with 287 recorded species, or 30.73% of the total number of species recorded on Mt. Fruška Gora. They are followed by the group of owlet moths with 268 recorded species (28.69% of the total number of species), geometrid moths with 187 recorded species (20.02% of the total number of species), butterflies (with 115 species or 12.31% of the total number of species), and macromoths (with 77 species or 8.24% of the total number of species). Of all Lepidoptera species recorded in Mt. Fruška Gora, 129 (or 13.80% of the total number of species) may be harmful for deciduous and coniferous trees. Research also revealed 61 species of Lepidoptera belonging to the group of migratory species. Seven species are first-order seasonal migratory species (eumigrators), one seasonal species is second-order migratory species (paramigrator), and 36 species are emigrants (first- and second-order local migrants). Finally, among the dismigrators there are two invasive species that expand their areas and five species that are possibly migratory. Regarding the recorded number of 934 species of Lepidoptera, it is estimated that this is just one third of the species that might exist on Mt. Fruška Gora and the rest could be recorded in the future. From Lepidoptera species recorded, 133 are new for the fauna of Serbia. For the first time in Serbia, but also in other former Yugoslav countries, a detailed analysis of chitinous armatures of genital apparatus with presenting specific taxonomic characteristics for 250 species (26.76% of the total number of recorded species), mostly for micromoths and some selected taxa. A comparative overview is given of species population number within families and superfamilies in investigated Fruška Gora area, as well as for Timoĉka Krajina, Europe, Romania, Romanian Banat, and Hungary. The order Lepidoptera is not sufficiently investigated in some areas of Mt. Fruška Gora. This primarily refers to the area south of the Danube River around Neštin, Susek, Banoštor, and Ĉerević, to the part between Sremski Karlovci, Petrovaradin and Ĉortanovci, from Krušedol and Neradin to Irig on the south slope of Mt. Fruška Gora, and from Vrdnik to Leţimir on the southwest slope (except the area around hunter’s house in the village of Grgurevci). Therefore, it is necessary to continue further faunistic and ecological research. A large number of localities are not explored enough, while at some microlocalities investigations have not even started, although they could be very interesting with possible new findings." ]

[ "info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43002/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "Lepidoptera", "Fruška gora", "fauna" ]

[ "Lepidoptera", "Mt. Fruška Gora", "fauna" ]

[ "Taksonomsko-faunistička studija leptira (Insecta : Lepidoptera) Fruške Gore", "Taxonomic-faunistical study of butterflies and moths (Insecta : Lepidoptera) of mt. Fruška Gora" ]

[ "" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/1937/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1937/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2045" ]

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[ "Dinić, Svetlana" ]

[ "Dinić, Svetlana", "Vidaković, Melita", "Vidaković, Melita", "Matić, Gordana" ]

[ "2020-02-24T09:14:53", "2020-07-03T08:08:16" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/11892", "http://eteze.bg.ac.rs/application/showtheses?thesesId=7230", "https://fedorabg.bg.ac.rs/fedora/get/o:20969/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=" ]

[ "Kičica se u Srbiji tradicionalno koristi za lečenje različitih oboljenja, uključujući i dijabetes. Cilj ovog rada bio je rasvetljavanje mehanizama antioksidativnog i protektivnog dejstva ekstrakta kičice (EK) koji doprinose očuvanju strukture i funkcije β-ćelija pankreasa i ublažavanju komplikacija u dijabetesu. Efekti EK analizirani su na eksperimentalnom modelu dijabetesa pacova izazvanom streptozotocinom (STZ) kao i na Rin-5F β-ćelijama izloženim oksidativnom stresu primenom vodonik peroksida, natrijum nitroprusida ili STZ-a. EK je ispoljio antidijabetogeno dejstvo uočeno kao sniženje nivoa glukoze/glikohemoglobina, poboljšanje lipidnog statusa i povećanje nivoa insulina u cirkulaciji dijabetičnih pacova što se može pripisati detektovanom uticaju EK na oc uvanje strukture Langerhansovih ostrvaca i povec anju mase β-c elija koje produkuju insulin. ntioksidativni efekat EK na β-c elije ogledao se u redukovanju oksidativnih os tec enja, modulisanju aktivnosti i ekspresije enzima antioksidativne zaštite (C T, SOD, GPx i GR) i uravnotežavanju aktivnosti redoks-senzitivnih faktora (NFκB, Nrf-2, Sp1, FOXO3 ) uključenih u regulaciju transkripcije gena za antioksidativne enzime. Dodatni doprinos EK boljem preživljavanju β-ćelija i sekreciji/ekspresiji insulina ostvaren je finim promenama u aktivnosti Akt, ERK i p38 kinaza kao i PDX-1 i MafA proteina. Pored toga, antioksidativni efekat EK kod dijabetičnih životinja ogledao se u zaštiti eritrocita, jetre i bubrega od gliko-oksidativnih oštećenja što je doprinelo poboljšanju njihovih funkcionalnih parametara. Opisani efekti EK ukazuju na značaj daljeg razjašnjavanja mehanizama antioksidativnog i protektivnog dejstva EK i njegovih komponenti u cilju potencijalnog terapijskog delovanja u dijabetesu." ]

[ "Centaury is traditionally used in Serbia for treating different diseases, including diabetes. This study aimed to elucidate the mechanisms of the antioxidant and protective effects of the centaury extract (CE) that preserve the structure and function of pancreatic β-cells and alleviate diabetic complications. The effects of the CE were analyzed on the experimental model of streptozotocin (STZ)-induced diabetic rats and in Rin-5F β-cells exposed to oxidative stress after exposure to hydrogen peroxide, sodium nitroprusside or STZ. The CE exhibited an anti-diabetic effect, observed as a decrease in hyperglycemia and glycohemoglobin concentration, an improved lipid status, and as increased insulin level in the circulation of diabetic rats. These effects can be attributed to the detected impact of the CE on the preservation of the structure of the islets of Langerhans, and on the increase in insulin producing β-cell mass. The antioxidant effect of the CE on β-cells was manifested as a reduction in oxidative damage, changed activities and expression of antioxidant enzymes, CAT, SOD, GPx and GR, and as a readjustment of the activities of redox-sensitive factors, NFκB, Nrf-2, Sp1, FOXO3A, involved in the regulation of gene transcription of antioxidant enzymes. Additional contributions of the CE to improved β-cell survival and insulin secretion/expression were achieved by the fine-tuning of the activities of Akt, ERK and p38 kinases and transcription regulators, PDX-1 and MafA. The antioxidant effect of the CE in diabetic animals was also reflected on increased protection of erythrocytes, liver and kidneys from glyco-oxidative damage, which contributed to the improvement of their functional parameters in diabetic rats. The described effects of the CE point to the importance of further clarification of the mechanisms of the antioxidant and general protective effects of the CE and its components in view of its potential use for therapeutic intervention in diabetes." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173020/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]

[ "kičica", "dijabetes", "pankreas", "β-ćelije", "oksidativni stres", "reaktivne vrste kiseonika/azota", "antioksidativna zaštita", "polifenoli", "komplikacije bolesti" ]

[ "centaury", "diabetes", "pancreas", "β-cells", "oxidative stress", "reactive oxygen/nitrogen species", "antioxidant protection", "polyphenols", "diabetic complications" ]

[ "Molekularni mehanizmi antioksidativnog dejstva ekstrakta kičice (Centaurium erythraea RAFN) u eksperimentalnom modelu dijabetesa pacova" ]

[ "Molecular mechanisms of the antioxidative effect of centaury (Centaurium erythraea RAFN), extract in the experimental model of diabetes in rats" ]

[ "BY-NC-SA" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/1842/IzvestajKomisije21928.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1841/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1841/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1842/IzvestajKomisije21928.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_11892" ]

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[ "Koko, Vesna" ]

[ "Manojlović-Stojanoski, Milica", "Milošević, Verica", "Šošić-Jurjević, Branka", "Ajdžanović, Vladimir" ]

[ "2016-01-05T11:45:33", "2020-07-03T08:08:25" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/2040", "http://eteze.bg.ac.rs/application/showtheses?thesesId=24", "https://fedorabg.bg.ac.rs/fedora/get/o:2269/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024496818" ]

[ "Izoflavoni soje, genistein i daidzein, se sve više koriste u preventivi i alternativnoj terapiji različitih patoloških stanja. Uticaj izoflavona soje na neuroendokrini sistem bio je, a i danas je, predmet istraživanja različitih studija. Veliki broj literaturnih podataka govori o uticaju izoflavona soje na reproduktivni sistem, s obzirom na njihovu estogensku i anti-estrogensku aktivnost. Ipak, podaci o njihovom uticaju na somatotropni i adrenokortikotropni sistem oskudni su, a neophodni, imajuću u vidu značaj ovih sistema za normalan razvoj i funkcionisanje organizma. Takoñe, mnogobrojne in vitro studije obezbeñuju samo limitirane informacije o potencijalnim efektima in vivo. Cilj ove studije je bio da se ispita uticaj hroničnih tretmana estradioldiproprionatom, genisteinom i daidzeinom na histološke, stereološke i biohemijske parametre somatotropnog i adrenokortikotropnog sistema orhidektomisanih adultnih pacova. Na početku eksperimenta adultni pacovi Wistar soja, stari 3 meseca, su podeljeni u dve eksperimentalne grupe: lažno orhidektomisanu (Sham) i orhidektomisanu (Orx) grupu. Dve nedelje nakon sham operacije ili orhidektomije pacovi su podeljeni u sedam eksperimentalnih grupa. Prvu (Sham1) grupu su sačinjavale sham operisane životinje subkutano (s.c.) tretirane odgovarajućom zapreminom sterilnog maslinovog ulja, medijumom estradiol-diproprionata (medijum I). Drugu (Orx1) grupu su sačinjavale orhidektomisane životinje s.c. tretirane odgovarajućom zapreminom medijuma I. U trećoj (Orx1+E) grupi orhidektomisane životinje su s.c. tretirane estradioldipropionatom u medijumu I (0,6 mg/kg t.m.). Četvrta (sham operisana - Sham2) i peta (orhidektomisana - Orx2) grupa s.c. su tretirane odgovarajućom zapreminom mešavine alkohola i sterilnog maslinovog ulja, medijumom genisteina i daidzeina (u odnosu 1:2; medijum II). Šestu (Orx2+G) grupu su sačinjavale orhidektomisane životinje s.c. tretirane genisteinom u medijumu II (30 mg/kg t.m.), dok je sedma (Orx2+D) grupa orhidektomisanih pacova s.c. tretirana daidzeinom u istom medijumu (30 mg/kg t.m.). Sve grupe životinja su dobijale tretman tokom tri nedelje (hroničan tretman), a žrtvovane su 24 h nakon poslednje primljene doze. Pre početka i nakon završenog tretmana izmerene su telesne mase životinja svih eksperimentalnih grupa, dok su mase hipofiza izmerene nakon žrtvovanja. Za lokalizaciju arkuatnog (Arc) i periventrikularnog (Pe) jedra, paravenrikularnog jedra (PVN) i eminencije medijane (ME) korišćeno je histološko bojenje krezil-ljubičastom bojom. Za obeležavanje oslobañajućih hormona hipotalamusa (oslobañajućeg hormona hormona rasta–GHRH, somatostatina-SS, kortikotropnog oslobañajućeg hormona-CRH) i ćelija adenohipofize (somatotropnih–GH i adrenokortikotropnih-ACTH) korišćeno je imunohistohemijsko bojenje. Histološki i imunohistohemijski obojeni preseci hipotalamusa, kao i imunohistohemijski obojeni preseci hipofiza stereološki su analizirani, uz pomoć newCAST stereološkog softverskog paketa. Dobijene su vrednosti: volumena Arc i Pe jedra i PVN, volumenske gustine SS neurona, volumena hipofize, volumenske i numeričke gustine GH i ACTH ćelija, apsolutnog broja i volumena GH i ACTH ćelija. Koncentracija GH i ACTH u cirkulaciji odreñena je biohemijskim metodama. Dobijeni podaci su statistički obrañeni..." ]

[ "The soy isoflavones genistein and daidzein are being used increasingly as preventive or alternative therapeutics in various pathological conditions. The effects of soy isoflavones on the neuroendocrine system were, and still are, the subject of numerous studies. Literature data predominantly focus on their effects on the gonadotrophic axis, considering the soy isoflavones established estrogenic and antiestrogenic mode of action. However, data about their potential effects on the somatotrophic and adrenocorticotrophic systems are rather scarce, but nevertheless very meaningful, considering the importance of these systems for the normal development and function of an organism. Furthermore, in vitro studies provide only limited data, that cannot be fully extrapolated to in vivo conditions. The aim of this study was to investigate the effects of chronically applied estradiol dipropionate, genistein and daidzein on the histological, stereological and biochemical parameters of somatotrophic and adrenocorticotrophic systems in orchidectomized adult rats. Initially, adult (three months old) Wistar rats were divided into two groups: sham operated (Sham) and orchidectomized (Orx). Two weeks after the surgery, the rats were subdivided into seven experimental groups. The first group (Sham1) included sham operated rats subcutaneously (s.c.) treated with an adequate volume of sterile olive oil, the medium for estradiol dipropionate (medium I). In the second group (Orx1) were orchidectomized rats s.c. treated with an adequate volume of medium I. The third group (Orx1+E) was comprised of orchidectomized rats s.c. treated with estradiol dipropionate in medium I (0.6 mg/kg b.w.). The fourth (sham operated – Sham2) and fifth (orchidectomized – Orx2) group of rats were s.c. treated with adequate volumes of absolute ethanol and sterile olive oil mixture, the medium for genistein or daidzein (mixing ratio was 1:2; medium II). In the sixth group (Orx2+G) were orchidectomized rats s.c. treated with genistein in medium II (30 mg/kg b.w.), while the seventh group (Orx2+D) included orchidectomized rats s.c. treated with daidzein in the same medium (30 mg/kg b.w.). All the groups of animals received the treatment during three weeks (chronic treatment), and were sacrificed 24 h after the last injected dose. All the animals were weighed before and after the treatment, while the pituitary weights were measured after sacrificing. For the localization of arcuate (Arc), periventricular (Pe) and paraventricular (PVN) nuclei, as well as for median eminence (ME) cresyl violet histochemical staining was used. Hypothalamic releasing hormones (growth hormone releasing hormone – GHRH, somatostatin – SS, corticotrophin releasing hormone – CRH) and pituitary cells (somatotrophic – GH and adrenocorticotrophic – ACTH) were immunohistochemically (peroxidase-antiperoxidase) labeled. Histochemically stained and immunohistochemically labeled hypothalamic sections, as well as immunohistochemically labeled pituitary sections were stereologically analysed, using newCast stereological software package. The following values were obtained: the Arc, Pe and PVN nuclei volumes, volume density of SS neurones, volumes of the pituitaries, the volume and numerical density of GH and ACTH cells, as well as the absolute numbers and volumes of GH and ACTH cells. The circulating GH and ACTH were determined biochemically. The obtained data were statistically processed..." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173009/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "Genistein", "daidzein", "GHRH", "SS", "GH ćelije", "CRH", "ACTH ćelije" ]

[ "Genistein", "daidzein", "GHRH", "SS", "GH cells", "CRH", "ACTH cells" ]

[ "Odgovor hipotalamo-hipofiznog sistema pacova na izoflavone soje : morfofunkcionalna studija", "Effects of soy isoflavones on the hypothalamic-pituitary system in rats: a morphofunctional study" ]

[ "" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/1886/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1886/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2040" ]

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[ "Tarasjev, Aleksej" ]

[ "Stevanović, Vladimir", "Miljković, Danijela", "Avramov, Stevan" ]

[ "2016-01-05T11:46:19", "2020-07-03T08:08:36" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/2065", "http://eteze.bg.ac.rs/application/showtheses?thesesId=151", "https://fedorabg.bg.ac.rs/fedora/get/o:5384/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=43481359" ]

[ "Promene u životnoj sredini od industrijske revolucije do danas su bez presedana u istoriji živog sveta i svojim razmerama prete da nadmaše adaptivni potencijal mnogih živih organizama, naročito biljaka, koje kao sesilni organizmi imaju samo ograničene mehanizme za izbegavanje stresa. Razumevanje kako biljke reaguju na ovakve promene, od subcelularnog do nivoa zajednica, je od krucijalnog značaja. U ovom radu smo ispitivali mogućnosti korišćenja analiza fluktuirajuće i radijalne asimetrije kao pokazatelja stabilnosti razvića vegetativnih i reproduktivnih organa kod dve zeljaste monokotile (Iris pumila i Iris germanica) i jedne drvenaste dikotile (Robinia pseudoacacia), u uslovima nenarušene životne sredine i u uslovima antropogeno uzrokovanog stresa (zagađenja u industrijskim i urbanim zonama). Analizirali smo mogućnosti korišćenja ovih analiza kao primarnih indikatora u sredinama sa negativnim antropogenim uticajem, kao i prednosti i mane ovog i nekih drugih metoda detekcije zagađenja. Stabilnost razvića procenjena putem fluktuirajuće i radijalne asimetrije se razlikuje između izučavanih sredina u slučaju sve tri vrste. Kod vrste R. pseudoacacia kao sredina u kojoj je detektovan najveći stepen razvojne nestabilnosti se izdvojio Kostolac (antropogeno degradirana sredina). Kod vrste I. pumila su biljke poreklom iz sredine pod antropogenim uticajem pokazale veću razvojnu nestabilnost kako u slučaju fluktuirajuće asimetrije, tako i u slučaju radijalne asimetrije. Kod vrste I. germanica su detektovane značajne razlike između zagađenja u slučaju indeksa radijalne asimetrije gde su biljke poreklom sa zagađenog staništa pokazale značajno veću razvojnu nestabilnost od biljaka poreklom sa nezagađenog staništa. Kod sve tri vrste je utvrđena značajna interakcija individue i asimetrije osobina, tj. da ekspresija razlika u fluktuirajućoj i radijalnoj asimetriji između ispitivanih osobina zavisi od klona tj. individue." ]

[ "Changes in the environment from the industrial revolution till present are unprecedented in the history of the life on Earth and their magnitude can overpower the adaptive potential of many living organisms, especially plants, that are sessile and have only limited mechanisms for avoiding stress. Understanding how plants respond to these changes, from subcellular to the community level, is crucial. In this study we investigated the possibility of using analysis of fluctuating and radial asymmetry as an indicator of developmental stability of vegetative and reproductive organs in two herbaceous monocots (Iris pumila and Iris germanica) and one wooden dikotile (Robinia pseudoacacia), in unpolluted environment, as well as in environment characterized by anthropogenically induced stress (industrial and urban zones pollution). We have analyzed the possibility of using this analysis as a primary indicator of pollution in areas with negative human impact, and the advantages and disadvantages of these and some other methods of pollution detection. Developmental stability estimated by fluctuating and radial asymmetry differed between the studied habitats for all three species. For R. pseudoacacia plants from Kostolac (polluted environment) had the highest degree of developmental instability. I. pumila plants originated from the environment under the influence of anthropogenic stress showed greater developmental instability in the case of fluctuating asymmetry, as well as radial asymmetry. In I. germanica species we detected significant differences between polluted and unpolluted habitats estimated by radial asymmetry where the plants originated from contaminated habitats showed significantly greater developmental instability. In all three species we detected significant interaction between individuals and trait asymmetries, e.g. expression of the difference in the radial and fluctuating asymmetry between the studied traits depended on the individual (clone)." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173025/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "Robinia pseudoacacia", "Iris pumila", "Iris germanica", "razvojna stabilnost", "radijalna asimetrija", "fluktuirajuća asimetrija", "biomonitoring" ]

[ "Robinia pseudoacacia", "Iris pumila", "Iris germanica", "developmental stability", "radial asymmetry", "fluctuating asymmetry", "biomonitoring" ]

[ "Fluktuirajuća asimetrija kao pokazatelj stabilnosti razvića odabranih biljnih taksona u uslovima nenarušene životne sredine i antropogeno indukovanog stresa", "Fluctuating asymmetry as an indicator of developmental stability of selected plant taxa in unpolluted environment and under anthropogenically induced stress" ]

[ "" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/1917/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1917/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2065" ]

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[ "Janaćković, Peđa" ]

[ "Marin, Petar", "Lakušić, Dmitar", "Zlatković, Bojan", "Rajčević, Nemanja" ]

[ "2020-02-07T10:29:23", "2020-07-03T08:08:13" ]

[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=7179", "https://nardus.mpn.gov.rs/handle/123456789/11825", "https://fedorabg.bg.ac.rs/fedora/get/o:20872/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025234354" ]

[ "Predstavnici kompleksa Centaurea atropurpurea (Acrocentron, Asteraceae) naseljavaju Balkan i južne Karpate. Varijabilnost taksona ovog kompleksa navodi na potrebu detaljnog istraživanja, kako bi se razjasnio njihov taksonomski status i bolje razumela filogenija grupe. Morfološke, fitohemijske, citološke i molekularne analize obuhvatile su 23 populacije vrste Centaurea calocephala sa celog areala, kao i 16 populacija ostalih taksona iz kompleksa Centaurea atropurpurea. Na osnovu horoloških podataka urađene su mape distribucije pojedinačnih taksona i istraživanog kompleksa. Opisana je nova vrsta za nauku – Centaurea zlatiborensis, endemična vrsta za Srbiju. Urađena je lektotipifikacija C. gjurasinii i C. crnogorica. Rezultati morfoloških analiza pokazali su veću varijabilnost karaktera stabljike i listova nego karaktera cvasti. Klaster analiza i CDA populacija C. calocephala pokazale su izdvajanje populacija: 1 (Lika) i 17 (Cluj-Napoca) na osnovu karaktera cvasti, dok su se populacije: 17 (Cluj-Napoca) i 7 (Đerdap) izdvojile na osnovu karaktera stabljike i listova. Fitohemijska ispitivanja isparljivih komponenti taksona kompleksa Centaurea atropurpurea pokazala su prisustvo dominantnih komponenti: germakrena D, E-kariofilena i kariofilen oksida. Na osnovu fitohemijskih markera u okviru taksona C. calocephala izdvojile su populacije 1 (Lika) i 20 (Cheile Turzii), dok su se taksoni C. zlatiborensis i C. crnogorica izdvojili od ostalih takona kompleksa. ETS analiza pokazala je monofiletsko poreklo Balkanske klade sek. Acrocentron. Uočena je introgresija i retikulacija koje su posledice homoploidne hibridizacije između taksona tokom ledenog doba u refungijumima na Balkanu. Na osnou ETS i AGT1 izdvojile su se grupe Calocephala A i B, kao i Grbavacensis grupa. Citološke analize pokazale su da svi istraživani taksoni iz kompleksa imaju diploidan broj hromozoma: 2n=20 i 2n=22." ]

[ "Representatives of Centaurea atropurpurea complex (Acrocentron, Asteraceae) are distributed on the Balkans and the southern Carpathians. The variability of the taxa of this complex indicates the need for detailed research, in order to clarify their taxonomic status and phylogeny. Morphological, phytochemical, cytological and molecular analyses included twenty-three populations of Centaurea calocephala and sixteen populations of other taxa from Centaurea atropurpurea complex. Based on the horological data, maps of the distribution for each taxon and the investigated complex were made. A new species for science has been described ‒ Centaurea zlatiborensis, an endemic species for Serbia. C. gjurasinii and C. crnogorica are lectotypified. The results of morphological analyses showed a greater variability of the stem and the leaves characters in comparison to the capitula characters. In cluster analysis and CDA population of C. calocephala separated from populations: 1 (Lika) and 17 (Cluj-Napoca) based on the characters of capitula, while the populations: 17 (Cluj-Napoca) and 7 (Djerdap) were separated based on the characters of the stem and leaves. Phytochemical analysis of the volatile compounds of the Centaurea atropurpurea complex showed the presence of the dominant components: germacrene D, E-caryophyllene and caryophyllene oxide. On the basis of the phytochemical markers, the populations of 1 (Lika) and 20 (Cheile Turzii) were separated within the C. calocephala, while the C. zlatiborensis and C. crnogorica separated from other taxa of the complex. ETS analysis showed the monophyletic origin of the Balkan clade of the sect. Acrocentron. Introgression and reticulation were observed, which are the consequences of homoploid hybridization between taxa during the ice age in Balkan refugia. ETS and AGT1 showed forming the groups Calocephala A and B, as well as the Grbavacensis group. Cytological analyses have shown that all investigated taxa from the complex have a diploid chromosome number: 2n = 20 and 2n = 22." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173029/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "Asteraceae", "listova i stabljike", "isparljive komponente", "ETS i AGT1", "broj hromozoma", "distribucija", "taksonomija", "filogenija", "kompleks Centaurea atropurpurea", "morfologija cvasti" ]

[ "Asteraceae", "leaves", "and stems", "volatile components", "ETS and AGT1", "chromosome number", "distribution", "taxonomy", "phylogeny", "complex Centaurea atropurpurea", "morphology of capitula" ]

[ "Morfološka, fitohemijska i molekularna istraživanja karpatsko - balkanskog kompleksa Centaurea atropurpurea (Asteraceae) - filogenetski i taksonomski aspekt" ]

[ "Morphological, phytochemical and molecular studies of Carpathian-Balkan complex Centaurea atropurpurea (Asteraceae) - phylogenetic and taxonomic implications" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/1828/IzvestajKomisije21835.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1827/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1827/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1828/IzvestajKomisije21835.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_11825" ]

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[ "Vandenbroucke, Roosmarijn" ]

[ "Kanazir, Selma", "Nedeljković, Nadežda", "Anđus, Pavle", "Libert, Claude" ]

[ "2020-02-14T14:30:25", "2020-07-03T08:08:07" ]

[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=7215", "https://nardus.mpn.gov.rs/handle/123456789/11836", "https://fedorabg.bg.ac.rs/fedora/get/o:20932/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51822095" ]

[ "" ]

[ "Neuroinflammation has been considered a common denominator and crucial player in neurodegeneration observed in various central nervous system (CNS) disorders. Considering the increasing evidence on the role of choroid plexus (CP) in neuroinflammatory processes through alterations in morphology and functionality of the choroid plexus epithelial (CPE) cells, the main goal of this thesis was to test the contribution of the CP in the initiation of neuroinflammation in two animal models of neuroinflammation-associated diseases: a model of Alzheimer’s disease, induced by intracerebroventricularly (i.c.v.) injected amyloid beta oligomers (AβO) and a lipopolysaccharide (LPS) induced sepsis animal model of systemic inflammation. Main findings from this research conclude that blood cerebrospinal fluid barrier (BCSFB) permeability is increased upon AβO injection, resulting from the loss of typical cuboidal morphology of CPE cells and a decrease in expression of tight junctions components. In the CP, upregulation of gene expression for various cytokines was observed, as well as their increased levels in the cerebrospinal fluid (CSF). Also, increase in gene expression for several matrix metalloproteinases (MMP) in the CP, and in MMP activity in the CSF was noted. After i.c.v. injection of broad spectrum MMP inhibitor with AβO, prevention of AβO-induced BCSFB permeability was found. In accordance with this, increase in BCSFB permeability upon AβO injection was observed in MMP3 deficient mice, but to a lesser extent. Furthermore, an increase in the number of particles in the CSF and an increase in gene expression of extracellular vesicles (EV) markers and miR-155 was found in the CP. A similar pattern of changes in the CP was observed in response to LPS injection compared to AβO injection. The results of this study revealed a significant role for the CP in the initiation of neuroinflammation, through structural and functional changes, in two different models associated with neuroinflammation." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173056/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]

[ "BCSFB", "CP", "MMP", "EV", "AβO", "neuroinflammation", "sepsis" ]

[ "KLB", "HP", "MMP", "EV", "AβO", "neuroinflamacija", "sepsa" ]

[ "Studies on structural and functional changes of mouse choroid plexus in the initiation of neuroinflammation" ]

[ "Значај структурних и функционалних промена хороидног плексуса миша у иницијацији неуроинфламације" ]

[ "BY-NC-SA" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/1806/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1807/IzvestajKomisije21878.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1806/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1807/IzvestajKomisije21878.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_11836" ]

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[ "Cvetković, Dragana" ]

[ "Blagojević, Jelena", "Jovanović, Slobodan" ]

[ "2016-01-05T11:45:32", "2020-07-03T08:08:38" ]

[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=33", "https://nardus.mpn.gov.rs/handle/123456789/2039", "https://fedorabg.bg.ac.rs/fedora/get/o:2320/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024510642" ]

[ "Vrste roda Mercurialis su se pokazale kao atraktivan objekat za raznovrsna ekološka, genetička i istraţivanja u evolucionoj biologiji biljaka. Šumski prosinac, Mercurialis perennis L., je višegodišnja zeljasta anemofilna i dvodoma vrsta, karakteristična za primarna ili manje narušena šumska staništa. Analiza populacija ove vrste sa različitih nadmorskih visina pruţila je mogućnost da se na relativno malom geografskom prostoru istraţi uticaj velikog raspona variranja ekoloških faktora na morfološke karakteristike i reproduktivnu alokaciju (RA) kod pripadnika različitih polova, kao i na brojčani odnos polova u populaciji. Istraţivanje je obuhvatilo populacije sa 14 lokaliteta u Srbiji, koje su uzorkovane u periodu 2006–2009. Statistički značajno odstupanje od pretpostavljenog ravnoteţnog odnosa polova ustanovljeno je u 69,5% populacionih uzoraka. Broj uzoraka sa neravnoteţnim odnosom polova varirao je meĎu godinama, a u većini istraţenih populacija neravnoteţa je bila u korist muških biljaka. Neravnoteţa u korist muških biljaka ustanovljena je i u istraţivanjima na drugim populacijama ove vrste, a ovakav obrazac neravnoteţe polova se smatra uobičajenim za višegodišnje dvodome biljke. Indeks polnog dimorfizma je kod većine analiziranih osobina imao male do umerne vrednosti, dok su izrazito i dosledno dimorfne bile one osobine koje su direktno povezane sa reprodukcijom – broj cvetova i masa cvasti. Smer polnog dimorfizma razlikuje se kod ovih osobina. Muški pol produkuje više cvetova, što ukazuje na pojačanu intraseksualnu selekciju u muškom polu za veću produkciju polena. S druge strane, masa ţenskih cvasti je višestruko veća od mase muških cvasti, što se preslikava i u veću reproduktivnu alokaciju ţenskih jedinki. U ţenskom polu je detektovan i obrazac smanjenja ulaganja u reprodukciju sa povećanjem nadmorske visine na kojoj biljka raste. Analize polnog dimorfizma u visini biljke pokazale su da je u većini populacionih uzoraka muški pol viši, što se uklapa u pretpostavke hipoteze disperzije polena..." ]

[ "The plant species of genus Mercurialis are very attractive object for various ecological, genetical and evolutionary researches. Dog’s mercury, Mercurialis perennis L., is a perennial anemophilous and dioecious herb, which usually grows in old and undisturbed forests. The analyses of populations of dog’s mercury from various altitudes gave us opportunity to investigate the effect of great environmental variation on morphological traits, reproductive allocation in different sexes, as well as on sex ratios in populations. This research included populations from 14 localities in Serbia sampled in 2006–2009. Statistically significant bias in sex ratio was found in 69.5% of population samples. Number of samples with biased sex ratio varied among years, and the majority of samples were male-biased. The male-biased populations of dog’s mercury were found elsewhere, and this pattern of bias is regarded to as the common one in dioecious perennials. Sexual dimorphism index for greater part of analysed traits had small to moderate values, while reproductive traits (number of flowers, mass of inflorescences) were markedly and consistently dimorphic. The range and direction of dimorphism differed in these reproductive traits. Males produced more flowers, and this information indicates pronounced intrasexual selection in males for greater pollen production. On the other hand, mass of female inflorescences was manifold bigger than mass of male inflorescences, and female reproductive allocation was bigger in a similar manner. In females, we also found pattern of decrease in reproductive allocation with the increase of altitude. The analyses of sexual dimorphism in plant size showed that males were the higher sex in majority of population samples. This is in agreement with pollen-dispersal hypothesis..." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173003/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]

[ "Mercurialis perennis", "odnos polova", "polni dimorfizam", "reproduktivno ulaganje", "evoluciona ekologija", "citogeografija" ]

[ "Mercurialis perennis", "sex ratio", "sexual dimorphism", "reproductive allocation", "evolutionary ecology", "cytogeography" ]

[ "Variranje odnosa polova, polnog dimorfizma i komponenti adaptivne vrednosti u populacijama Mercurialis perennis L. (Euphoriaceae) duž gradijenta nadmorske visine", "Variation in sex ratio, sexual dimorphism, and fitness components in populations of Mercurialis perennis L. (Euphorbiaceae) along the altitudinal gradient" ]

[ "" ]

[ "BY-NC-SA" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/1925/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1925/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2039" ]

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[ "Korićanac, Goran" ]

[ "Cvijić, Gordana", "Žakula, Zorica" ]

[ "2016-01-05T11:45:51", "2020-07-03T08:08:40" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/2044", "http://eteze.bg.ac.rs/application/showtheses?thesesId=11", "https://fedorabg.bg.ac.rs/fedora/get/o:2124/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=" ]

[ "Insulin i estradiol imaju va.nu ulogu u regulaciji metabolizma ugljenih hidrata i lipida. Razli.ita klini.ka istra.ivanja i eksperimentalni podaci ukazuju da varijacije u koncentraciji estrogena uti.u na insulinsko delovanje. Cilj ove studije je bilo pore.enje uticaja estradiola na po.etne molekule insulinskog signalnog puta u glavnim ciljnim tkivima za insulin (jetra) i estradiol (uterus), kao i u srcu, u kome oba hormona ostvaruju zna.ajne efekte. Ovarijektomisane .enke pacova su tretirane estradiolom 6 h pre analize sadr.aja proteina i iRNK molekula signalnog puta insulina. Da bi se istakli efekti estradiola na fosforilacije i asocijacije molekula relevantne za insulinski signalni put, .ivotinje su dodatno injecirane insulinom 30 min pre eksperimenta. Tretman estradiolom nije promenio nivo insulina i glukoze u plazmi, ali je doveo do zna.ajnog smanjenja nivoa slobodnih masnih kiselina i pove.anja te.ine uterusa. U jetri, tretman estradiolom je doveo do smanjenja fosforilacije IR, kao i smanjenja proteinskog sadr.aja IRS-1, .to navodi na zaklju.ak da je estradiol suprimirao efekte insulina preko IR/IRS-1 puta i verovatno ih usmerio na alternativni put, .to je potkrepljeno i pove.anjem proteinskog sadr.aja IRS-2. U uterusu je tretman estradiolom nakon 6 h doveo do statisti.ki zna.ajnog pove.anja proteinskog sadr.aja skoro svih ispitivanih molekula signalnog puta insulina. U srcu je estradiol uzrokovao pove.anje asocijacije IRS-1/p85, pove.anja sadr.aja proteina i iRNK p85, kao i pove.anje fosforilacije Akt na Ser473. S druge strane, tretman estradiolom je u srcu izazvao i smanjenje fosforilacije tirozina IR, smanjenje proteinskog sadr.aja IRS-2 i iRNK oba IRS proteina. Rezultati ove studije ukazuju na to da tretman estradiolom indukuje tkivno specifi.ne promene u insulinskom signalnom putu. Posledice tretmana estradiolom na molekule insulinskog signalnog puta su o.iglednije u uterusu, ali je njihov fiziolo.ki zna.aj za insulinsko delovanje verovatno ve.i u jetri. S druge strane, rezultati dobijeni u srcu sugeri.u veoma kompleksnu ulogu estradiola u fiziologiji srca, koja se manifestuje i kombinacijom pozitivnog i negativnog, genomskog i negenomskog delovanja ovog hormona na molekule signalnog puta insulina." ]

[ "Insulin and estradiol play important role in regulation of carbohydrate and lipid metabolism. Various clinical observations and experimental data suggest that variations in the concentration of estrogens affect insulin action. The aim of the present study was to compare the impact of estradiol on early steps of insulin signaling in main target tissues of insulin (the liver) and estradiol (the uterus), and in the heart, where both hormones realize important effects. Ovariectomized female rats were treated with estradiol 6 h prior to analysis of protein and mRNA content of insulin signaling molecules. To delineate estradiol effects on phosphorylations and molecular associations relevant for insulin signaling, animals were treated additionally with insulin 30 min before the experiment. Treatment with estradiol did not change the levels of plasma insulin and glucose, but it significantly decreased the free fatty acid level and increased uterine weight. In liver, estradiol treatment decreased IR phosphorylation and IRS-1 protein content, suggesting that estradiol suppressed insulin action through IR/IRS-1 and probably redirected it to alternative pathway. This conclusion is supported with increase of IRS-2 protein content. In uterus, estradiol treatment resulted in significant increase of protein content of almost all analyzed molecules. In heart, estradiol increased IRS-1/p85 association, p85 protein and mRNA level, and Ser473Akt phosphorylation. On the other hand, estradiol treatment decreased tyrosine phosphorylation of cardiac IR, protein content of IRS-2, and mRNA of both IRS proteins. These results suggest that estradiol treatment induces tissue-specific changes in insulin signaling. Consequences of estradiol treatment on insulin signaling molecules are more apparent in uterus, but their physiological relevance for insulin action is probably more important in liver. On the other hand, results obtained in heart suggest very complex role of estradiol in heart physiology, represented by, amongst other effects, combination of positive and negative, genomic and nongenomic actions of this hormone on insulin signaling molecules." ]

[ "info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41009/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "Estradiol", "insulinski signalni put", "jetra", "uterus", "srce" ]

[ "Estradiol", "insulin signaling", "liver", "uterus", "heart" ]

[ "Tkivna specifičnost efekata 17ß-estradiola na signalni put insulina", "Tissue specificity of 17ß-estradiol effects on insuli signaling pathway." ]

[ "" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/1933/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1933/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2044" ]

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[ "Đorđević, Valentina" ]

[ "Radojković, Dragica", "Đorđević, Valentina", "Radojković, Dragica", "Savić-Pavićević, Dušanka" ]

[ "2017-07-10T15:03:06", "2020-07-03T08:08:21" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/8364", "http://eteze.bg.ac.rs/application/showtheses?thesesId=5098", "https://fedorabg.bg.ac.rs/fedora/get/o:15910/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025154994" ]

[ "Protrombin predstavlja zimogen trombina (koagulacinog faktora II), koji ima centralnu ulogu u održanju hemostazne ravnoteže. Zahvaljujući alosteričnoj regulaciji, trombin može da vrši i prokoagulantnu i antikoagulantnu funkciju. Region 3' kraja gena za protrombin ima nekanonsku organizaciju i podložan je nastanku genskih varijanti koje mogu imati značajnu ulogu u regulaciji ekspresije i funkcije protrombina. Do sada opisane varijante u ovom regionu su povezivane sa povišenom ekspresijom protrombina koja može dovesti do hiperkoagulacije i povećane sklonosti ka trombozama, odnosno trombofiliji. U Laboratoriji za molekularnu biologiju, Instituta za molekularnu genetiku i genetičko inženjerstvo, u 3' kraju gena za protrombin opisane su FIIc.1787G>A i FIIc.*64_*66del varijante čiji mehanizmi do sada nisu rasvetljeni. Varijanta FIIc.1787G>A (protrombin Beograd) se nalazi u poslednjem egzonu gena za protrombin i dovodi do aminokiselinske zamene Arg596Gln u proteinu. Ova varijanta se nalazi u regionu za koji se vezuju joni natrijuma, neophodni za prokoagulantnu aktivnost trombina, kao i prirodni trombinski inhibitor- antitrombin. Varijanta FIIc.*64_*66del se nalazi u 3' netranslatirajućem regionu gena i predstavlja deleciju 3 nukleotida. Imajući u vidu pozicije u različitim strukturnim elementima 3' kraja gena, pretpostavlja se da su mehanizmi delovanja ovih varijanti različiti. Prvi deo studije je imao za cilj određivanje učestalosti FIIc.1787G>A i FIIc.*64_*66del varijanti kod 353 pacijenta sa različitim trombotičkim poremećajima i 250 zdravih ispitanika u srpskoj populaciji. Pacijenti su u zavisnosti od tipa trombotičkog poremećaja podeljeni u 4 grupe: tromboze dubokih vena (94 pacijenta), izolovani plućni embolizam (104 pacijenta), kombinovani trombotički poremećaji (49 pacijenata) i spontani pobačaji (106 pacijentkinja). Drugi deo studije je obuhvatio ex vivo analize, s ciljem da se ispita uticaj navedenih varijanti na funkcionalnost i raspoloživu količinu protrombina u plazmi ispitanika..." ]

[ "Prothrombin is a zymogen of thrombin (coagulation factor II), which plays a central role in maintaining hemostatic balance. Due to the allosteric regulation, thrombin has dual function: procoagulant and anticoagulant. The 3' end of prothrombin gene has noncanonical organization, which is susceptible to the genetic variants that might have a significant role in the regulation of prothrombin expression and function. Variants reported within this region lead to increased prothrombin expression which is associated with hypercoagulability and increased tendency to thrombophilia. In the Laboratory for Molecular Biology at the Institute of Molecular Genetics and Genetic Engineering, we have reported FIIc.1787G>A and FIIc.*64_*66del variants in 3' end of prothrombin gene, but their mechanisms of action have not been elucidated so far. The FIIc.1787G>A (prothrombin Belgrade) is located in the last exon of the prothrombin gene, leading to amino acid substitution Arg596Gln in the protein. This variant is positioned in a region of antithrombin (thrombin inhibitor) binding site, as well as in Na+ binding loop, which is significant for procoagulant activity of thrombin. The FIIc.*64_*66del is located in the 3' untranslated gene region and represents a deletion of three nucleotides. Taking into account the position of these two variants in different structural elements of 3' end of prothrombin gene, it is assumed that they differ in the mechanisms of action. In the first part of the study, we determined the frequency of FIIc.1787G>A and FIIc.*64_*66del variants in 353 patients with thrombotic disorders and 250 healthy subjects in Serbian population. Patients were divided into 4 groups according to the type of thrombotic disorders: deep vein thrombosis (94 patients), isolated pulmonary embolism (104 patients), combined thrombotic disorders (49 patients) and fetal loss (106 patients). The second part of the study included the ex vivo analyses, with aim to investigate the impact of these variants on the functionality and available amount of prothrombin in the subjects plasma samples..." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173008/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "FIIc.1787G>A", "protrombin Beograd", "FIIc.*64_*66del", "protrombin", "3' kraj gena", "antitrombin rezistencija", "regulacija genske ekspresije", "thrombofilija" ]

[ "FIIc.1787G>A", "prothrombin Belgrade", "FIIc.*64_*66del", "prothrombin", "3end of gene", "antithrombin resistance", "gene expression regulation", "thrombophilia" ]

[ "Funkcionalna analiza genskih varijanti FIIc.1787G>A (protrombin, Beograd) i FIIc.*64_*66del i njihova povezanost sa trombofolijom" ]

[ "Functional analysis of FIIc.1787G>A (protrombin, Belgrade) and FIIc.*64_*66del gene variants and their assotiation with trombophilia" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/1869/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1870/IzvestajKomisije11048.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1869/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1870/IzvestajKomisije11048.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_8364" ]

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[ "Kanazir, Selma" ]

[ "Nedeljković, Nadežda", "Pešić, Vesna", "Ruždijić, Sabera", "Perović, Milka" ]

[ "2016-01-05T11:45:33", "2020-07-03T08:08:29" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/2042", "http://eteze.bg.ac.rs/application/showtheses?thesesId=39", "https://fedorabg.bg.ac.rs/fedora/get/o:2348/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=41539855" ]

[ "Traumatska povreda mozga (engl. traumatic brain injury, TBI) predstavlja povredu moždanog tkiva uzrokovanu mehaničkom povredom glave. TBI je jedan od vodećih uzroka smrtnosti i invaliditeta u svetu, u populaciji ljudi do 45 godina starosti. Posledice TBI zavise od lokalizacije i količine oštećenja moždanog tkiva i mogu varirati od blažih motoričkih i kognitivnih smetnji, pa sve do težih oblika invaliditeta i smrti. Traumatska povreda mozga dovodi do narušavanja krvno-moždane barijere, što rezultuje tačno određenim sledom događaja u povređenom tkivu CNS-a. Naime, akutna faza, označena kao primarna povreda, nastaje direktnim delovanjem mehaničke sile na moždano tkivo i javlja se u trenutku povrede. Događaji koji slede nakon toga označeni su kao sekundarna povreda koja nastaje kao posledica inicijalnog oštećenja, a obuhvata procese koji dovode do daljeg oštećenja tkiva u danima i nedeljama nakon povrede. To su pre svega zapaljenski procesi koji dovode do smrti neurona koji prvobitno nisu bili zahvaćeni mehaničkom povredom. Smatra se da je broj neurona koji umre usled širenja sekundarne povrede daleko veći od broja neurona koji strada usled primarne povrede. Činjenica da se većina procesa u okviru sekundarne povrede odigrava relativno kasno (satima i danima nakon povrede) čini ih podložnim različitim terapeutskim intervencijama. Već decenijama je poznato da restrikcija hrane povoljno deluje na čitav organizam tako što odlaže starosno-zavisne fiziološke promene i smanjuje incidencu različitih obolenja (kancer, autoimunske bolesti, Parkinsonova i Alchajmerova bolest, itd.) Međutim, tek u poslednjih desetak godina intenzivno se istražuje uticaj smanjenog unosa hrane na procese oporavka nakon povrede. Dosadašnja istraživanja na ovom polju su pokazala da restrikcija hrane u trajanju od nekoliko meseci pre povrede CNS-a ima neuroprotektivno dejstvo i promoviše funkcionalni oporavak u nekoliko različitih modela povrede..." ]

[ "Traumatic brain injury (TBI) represents a brain tissue injury caused by mechanical head injury. TBI is one of the leading causes of death and disability in the world human population, up to 45 years of age. Consequences of brain injury depend on the location and amount of brain tissue damage, and can range from mild motor and cognitive impairment, to severe forms of disability and death. Traumatic brain injury leads to impairment of blood-brain barrier, resulting in a precisely defined sequence of events in injured CNS tissue. Two main phases could be distinguished: acute phase, which is called the primary injury that occurs by direct action of mechanical forces on the brain tissue and occurs at the time of injury. The events that followed thereafter are called the secondary injury. Secondary injury occurs as a result of the initial damage, and includes the processes that lead to further tissue damage in the days and weeks following injury. These are primarily inflammatory processes that lead to the death of neurons that initially were not affected by mechanical injury. It is believed that the neuronal cell death caused by secondary injury is far greater than the one caused by primary injury. The fact that most of the processes within the secondary injury occur relatively late (hours and days following injury) makes them subject to various therapeutic interventions. For decades, it is known that food restriction has a beneficial effect on the entire organism by delaying age-dependent physiological changes and reducing the incidence of various diseases (cancer, autoimmune disease, Parkinson's and Alzheimer's, etc). However, only in the last ten years has been to investigate the influence reduced food intake in the recovery process following injury. Previous research in this field has shown that several months long food restriction prior to CNS injury has neuroprotective effects and promotes functional recovery in several different models of injury..." ]