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https://nardus.mpn.gov.rs/handle/123456789/9381
123456789-9381.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:17526/bdef:Content/download
no
Medonosna pčela (Apis mellifera) kao bioindikator zagađenja toksičnim metalima na teritoriji Srbije
Honeybees (Apis mellifera) as bioindicators of toxic metal pollution in Serbia.
[ "Stanisavljević, Ljubiša" ]
[ "Gržetić, Ivan", "Krpo-Ćetković, Jasmina", "Jovančićević, Branimir", "Stanisavljević, Ljubiša" ]
Zarić, Nenad M.
2018-04-23T09:30:07
[ "2018-04-23T09:30:07", "2020-07-03T08:06:08" ]
2017-12-21
[ "https://nardus.mpn.gov.rs/handle/123456789/9381", "http://eteze.bg.ac.rs/application/showtheses?thesesId=5721", "https://fedorabg.bg.ac.rs/fedora/get/o:17526/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=49969935" ]
[ "Povećanje broja stanovnika na Zemlji neminovno dovodi i do povećanja antropogenog zagađenja. Metali se smatraju jednim od najopasnijih zagađivača današnjice, posebno zbog toga što mnogi od njih ne podležu biodegradaciji. Uzimajući ovo u obzir, neophodno je pronaći metode za njihovo praćenje u životnoj sredini koje su jeftinije, pokrivaju veća područja i daju brže rezultate. Jedna od alternativa klasičnim tehnikama merenja jeste upotreba bioindikatorskih organizama. U ovom radu korišćena je medonosna pčela (Apis mellifera L.) kao bioindikator zagađenja toksičnim metalima na teritoriji Srbije. U telima medonosnih pčela sakupljenih iz pčelinjaka u Beogradu, Kostolcu i Mesiću tokom dva meseca (jul i septembar) 2014. godine analizirani su Al, Ba, Cd, Co, Cr, Cu, Fe, Li, Mn, Na, Ni, Pb, Sr i Zn. U telima pčela uzorkovanih u Pančevu i Vršcu tokom septembra 2013. godine, jula i septembra 2014. i jula i septembra 2015. godine analizirani su Al, Ba, Cd, Co, Cr, Cu, Fe, Mn, Na, Ni, Sr i Zn. Pčele su uzorkovane u ovom periodu jer je njihova aktivnost usko vezana za vremenske prilike, a ovа dva meseca predstavljaju vrhunac te aktivnosti. Isti metali su analizirani i u uzorcima zemljišta uzetih u blizini pčelinjaka u Pančevu i Vršcu. Za određivanje koncentracije ovih metala korišćena je optička emisiona spektrometrija sa induktivno spregnutom plazmom (ICP-OES). Dvosmerna ANOVA je pokazala da postoje statistički značajne razlike između koncentracija pojedinih metala u Beogradu, Kostolcu i Mesiću. Značajne razlike između bar dve lokacije ustanovljene su za Al, Ba, Cr, Cu, Fe, Li i Ni. Korišćenjem analize glavnih komponenti (PCA), klasterske analize (CA) i ANOVA-e, ustanovljeno je da povećane koncentracije Al, Fe i Cr u okolini Kostolca potiču iz termoelektrana „Kostolac A i B“ i vi pripadajućeg pepelišta..." ]
[ "The increase of the human population on Earth has led to the increase of anthropogenic pollution. Toxic metals are considered one of the most dangerous pollutants because many of them are not biodegradable. With this in mind, it is important to find monitoring methods that cost less, cover bigger areas and give faster results. An alternative to classical monitoring techniques is the use of bioindicators. For the purposes of this study, honeybees (Apis mellifera L.) were used as bioindicators of toxic metal pollution in Serbia. One set of samples were adult honeybees collected during two sampling periods (July and September) in 2014 from apiaries located in Belgrade, Kostolac, and Mesić. Analyzed metals in these samples were Al, Ba, Cd, Co, Cr, Cu, Fe, Li, Mn, Na, Ni, Pb, Sr, and Zn. Another set of samples were honeybees from apiaries located in Pančevo and Vršac that were sampled during September 2013, July and September 2014, and July and September 2015. The bees were sampled during these two months because they represent the peak of bee activity. Metals analyzed in these samples were Al, Ba, Cd, Co, Cr, Cu, Fe, Mn, Na, Ni, Sr, and Zn. The same metals were analyzed in soil samples taken from Pančevo and Vršac in the vicinity of apiaries. For the quantification of analyzed metals, inductively coupled plasma – optical emission spectrometry (ICP-OES) was used. The two-way ANOVA showed that there are statistically significant differences between some of the analyzed metals in Belgrade, Kostolac, and Mesić. Significant differences between at least two of the locations were found for Al, Ba, Cr, Cu, Fe, Li, and Ni. Using principal component anlasys (PCA), cluster analysis, and ANOVA, it was determined that higher concentrations of Al, Fe, and Cr in Kostolac originate from thermal ix power plants “Kostolac A and B” and the accompanying ash disposal site..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/176006/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc/4.0/" ]
Универзитет у Београду
[ "monitoring", "medonosna pčela", "biomonitoring", "bioindikatori", "toksični metali", "zagađenje" ]
[ "monitoring", "honeybee", "biomonitoring", "bioindicator", "toxic metals", "pollution" ]
[ "Medonosna pčela (Apis mellifera) kao bioindikator zagađenja toksičnim metalima na teritoriji Srbije" ]
[ "Honeybees (Apis mellifera) as bioindicators of toxic metal pollution in Serbia." ]
doctoralThesis
[ "BY-NC" ]
Станисављевић, Љубиша; Станисављевић, Љубиша; Гржетић, Иван; Крпо-Ћетковић, Јасмина; Јованчићевић, Бранимир; Зарић, Ненад М.; Медоносна пчела (Aпис меллифера) као биоиндикатор загађења токсичним металима на територији Србије; Медоносна пчела (Aпис меллифера) као биоиндикатор загађења токсичним металима на територији Србије;
[ "http://nardus.mpn.gov.rs/bitstream/id/1282/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1283/IzvestajKomisije16024.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1283/IzvestajKomisije16024.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1282/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_9381" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/9380
123456789-9380.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:17451/bdef:Content/download
no
Rasprostranjenje, diverzitet i struktura zajednica ektoparazita litofilnih slepih miševa (Chiroptera) centralnog Balkana
Distribution, diversity and structure of ectoparasite communities of cave-dweeling bats (Chiroptera) from the central Balkans
[ "Ćirović, Duško" ]
[ "Tomanović, Snežana", "Petrović, Anđeljko", "Ćirović, Duško", "Tomanović, Snežana" ]
Burazerović, Jelena O.
2018-04-23T09:30:07
[ "2018-04-23T09:30:07", "2020-07-03T08:06:07" ]
2017-12-29
[ "https://nardus.mpn.gov.rs/handle/123456789/9380", "http://eteze.bg.ac.rs/application/showtheses?thesesId=5706", "https://fedorabg.bg.ac.rs/fedora/get/o:17451/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=49963279" ]
[ "Studija predstavlja prvo sveobuhvatno istraživanje faune, brojnosti, učestalosti i specifičnosti različitih vrsta ektoparazita u odnosu na vrstu domaćina, kao i analizu ekoloških faktora koji utiču na brojnost i reprodukciju ektoparazita litofilnih slepih miševa na teritoriji centralnog Balkana. Trogodišnje terensko istraživanje je sprovedeno na 45 lokaliteta u Srbiji, Bosni i Hercegovini, Bivšoj Jugoslovenskoj Republici Makedoniji i Crnoj Gori. Prikupljeno je i analizirano preko 3500 jedinki ektoparazita klasifikovanih u 21 vrstu: tri vrste krpelja, sedam vrsta parazitskih grinja (uključujući jednog predstavnika familije Trombiculidae), osam vrsta parazitskih muva i tri vrste buva, od čega je najveći broj nalaza prvi za teritorije istraživanih država. Identifikovano je 75 asocijacija ektoparazit-domaćin. Veći broj vrsta ektoparazita slepih miševa identifikovane su kao specijalisti. Miniopterus schreibersii je domaćin sa najvećim brojem vrsta ektoparazita. Najrasprostranjenije vrste ektoparazita u studiji su bile Spinturnix psi i Penicillidia dufourii. Kod tri vrste slepih miševa (Miniopterus schreibersii, Myotis myotis i Rhinolophus ferrumequinum) utvrđeno je da postoji statistički značajan efekat pola domaćina na brojnost ektoparazita. Faza životnog ciklusa, pol, starost i reproduktivni status domaćina značajno su uticali na brojnost i reproduktivnu aktivnost vrsta ektoparazita karakterističnih za Miniopterus schreibersii. Osim originalnih podataka, studijom su obuhvaćeni i analizirani do sada objavljeni literaturni podaci o rasprostranjenju i domaćinima ektoparazita slepih miševa na istraživanoj teritoriji." ]
[ "The study represents first comprehensive research of fauna, abundance, prevalence and host specificity, as well as analysis of ecological factors influencing abundance and reproduction of ectoparasites parasitizing cave-dwelling bats at the territory of the central Balkans. Three years long field research was conducted at 45 localities in Serbia, Bosnia and Herzegovina, former Yugoslav Republic of Macedonia, and Montenegro. Over 3,500 ectoparasite specimens belonging to 21 species were identified: three species of ticks, seven species of mites (including one specimen of Trombiculidae family), eight species of bat flies and three species of fleas, most being the first records at the territory of investigated countries. A total of 75 host-parasite associations were identified. A high level of host specificity in analysed ectoparasite species was registered. Miniopterus schreibersii was host species with the greatest number of ectoparasite species. Mite Spinturnix psi and bat fly Penicillidia dufourii were the most widely distributed species. There was significant effect of host sex on ectoparasite abundance in three bat species (Miniopterus schreibersii, Myotis myotis and Rhinolophus ferrumequinum). Significant effects of host life cycle, age, sex and reproductive status on total number and reproductive activity were identified in the case of ectoparasite species characteristic for Miniopterus schreibersii. Besides original data, the study includes and analyses published literature data about ectoparasite hosts and distribution of bat ectoparasites at the studied territory." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173006/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "ektoparaziti", "slepi miševi", "centralni Balkan", "asociranost", "sezonska dinamika ektoparazita" ]
[ "ectoparasites", "bats", "central Balkans", "associations", "seasonal dynamics of ectoparasites" ]
[ "Rasprostranjenje, diverzitet i struktura zajednica ektoparazita litofilnih slepih miševa (Chiroptera) centralnog Balkana" ]
[ "Distribution, diversity and structure of ectoparasite communities of cave-dweeling bats (Chiroptera) from the central Balkans" ]
doctoralThesis
[ "BY-NC-ND" ]
Ћировић, Душко; Петровић, Aнђељко; Ћировић, Душко; Томановић, Снежана; Томановић, Снежана; Буразеровић, Јелена О.; Распрострањење, диверзитет и структура заједница ектопаразита литофилних слепих мишева (Цхироптера) централног Балкана; Распрострањење, диверзитет и структура заједница ектопаразита литофилних слепих мишева (Цхироптера) централног Балкана;
[ "https://nardus.mpn.gov.rs/bitstream/id/1276/IzvestajKomisije16010.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1275/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1275/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1276/IzvestajKomisije16010.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_9380" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/7948
123456789-7948.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:15161/bdef:Content/download
no
Rečna mrena (Barbus barbus) kao bioindikator zagađenja rečnih sedimenata metalima
Common barbel (Barbus barbus ) as a bioindicator of river sediment pollution with metals
[ "Krpo-Ćetković, Jasmina" ]
[ "Đikanović, Vesna", "Krpo-Ćetković, Jasmina", "Spasić, Slađana", "Rašković, Božidar" ]
Morina, Arian G.
2017-04-22T09:33:48
[ "2017-04-22T09:33:48", "2020-07-03T08:05:49" ]
2016-12-30
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=4834", "https://nardus.mpn.gov.rs/handle/123456789/7948", "https://fedorabg.bg.ac.rs/fedora/get/o:15161/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025149362" ]
[ "Metali spadaju među značajne zagađivače akvatičnih ekosistema, s obzirom na njihovu stabilnost u ekosistemu i transport kroz lanac ishrane. Rečni sedimenti mogu deponovati visoke koncentracije metala, a brojni akvatični organizmi koriste sediment za različite potrebe u različitim fazama svog životnog ciklusa. U ovom radu analizirane su ukupna i dostupna frakcija 15 elemenata u površinskim sedimentima Dunava, Zapadne Morave i Južne Morave, u tri sezone 2012. godine (april, jul, novembar), na tri različite dubine sedimenta (0-5 cm, 5-10 cm, 10-15 cm), uz upotrebu optičke emisione spektrometrije sa induktivno spregnutom plazmom (ICP-OES). Koncentracije elemenata utvrđene su i u tkivima rečne mrene (Barbus barbus) uzorkovane iz sve tri analizirane reke, i to u mišiću, škrgama, jetri i crevu. Analiza koncentracije metala obuhvatila je i intestinalnog parazita Pomphorhynchus laevis (Acanthocephala) izolovanom iz mrene iz Dunava. Histološke analize urađene su u cilju utvrđivanja promena na škrgama i jetri mrene iz Dunava i Južne Morave. Za dodatnu analizu elemenata u sedimentu Zapadne Morave, u tkivima mrene iz Dunava, kao i u parazitu P. laevis, korišćena je skenirajuća elektronska mikroskopija sa energodisperzivnom spektroskopijom (SEM-EDS). Statistička analiza i trofaktorska MANOVA pokazale su postojanje značajnih razlikau veličini čestica sedimenta, prosečnoj koncentraciji elemenata i sezonskim promenama u koncentraciji elemenata između tri posmatrane reke. Koncentracije Cu i Ni u Zapadnoj Moravi i Dunavu bile su veće od maksimalno dozvoljenih koncentracija (MDK) za sediment u Republici Srbiji, dok je koncentracija Hg bila veća od MDK u sve tri reke. Koncentracije Al, Ba, Cu, Fe, Mn i Zn varirale su između sezona u Zapadnoj Moravi i Dunavu, sa najvećim vrednostima u letnjem periodu. U Južnoj Moravi varirale su koncentracije Al, As, Fe, Mn i Zn sa najmanjim koncentracijama u letnjem periodu. Zapadna Morava je sadržala najveće količine finih čestica u svom sedimentu, dok se najveći deo sedimenta Južne Morave sastojao od čestica peska..." ]
[ "Metals are listed among the most important pollutants in aquatic ecosystems due to their stability and mobility through the food webs. River sediments can contain high concentrations of metals, and various aquatic organisms use sediments for various requirements in different stages of their life cycle. In this research, total and bioavailable concentrations of 15 elements in surface sediments of three rivers – the Danube, the Zapadna Morava, and the Južna Morava, were analyzed during three seasons in 2012 (April, July, November), at three different sediment depths (0-5 cm, 5-10 cm, 10-15 cm), using inductively coupled plasma optical emission spectrometry (ICP-OES). Element concentrations were also determined in 4 tissues of the common barbel (Barbus barbus) sampled from all three rivers (muscle, gills, intestine, and liver). Element analysis was further carried out on barbel intestinal parasite Pomphorhynchus laevis (Acanthocephala), collected from the Danube River. Histological analyses of gills and liver from fish collected from the Danube and the Južna Morava was performed with the purpose of determining any tissue alterations. Scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM-EDS) was used for in-depth grain analysis of the Zapadna Morava sediments, as well as tissue and parasite P. laevis analyses of elements in barbel sampled from the Danube River. Statistical analysis and three-way MANOVA showed significant differences in sediment grain size, average element concentration, and seasonal element concentration in the three rivers. Concentrations of Cu and Ni in the Zapadna Morava River and the Danube River were above the maximum allowable concentrations (MAC) of elements in river sediments for the Republic of Serbia, while Hg concentration was above the MAC in all three observed rivers. Concentrations of Al, Ba, Cu, Fe, Mn, and Zn varied between seasons in the Zapadna Morava and the Danube, with the highest values during the summer season. Concentrations of Al, As, Fe, Mn, and Zn varied between seasons in the Južna Morava, with the lowest values during the summer season..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc/4.0/" ]
Универзитет у Београду
[ "ukupni i dostupni metali", "sediment", "Dunav", "Zapadna Morava", "Južna Morava", "rečna mrena", "mišić", "jetra", "škrge", "creva", "intestinalni paraziti" ]
[ "total and available metals", "sediment", "the Danube", "the Zapadna Morava", "the Južna Morava", "common barbel", "muscle", "liver", "gills", "intestine", "intestinal parasites" ]
[ "Rečna mrena (Barbus barbus) kao bioindikator zagađenja rečnih sedimenata metalima" ]
[ "Common barbel (Barbus barbus ) as a bioindicator of river sediment pollution with metals" ]
doctoralThesis
[ "BY-NC" ]
Крпо-Ћетковић, Јасмина; Крпо-Ћетковић, Јасмина; Спасић, Слађана; Рашковић, Божидар; Ђикановић, Весна; Морина, Aриан Г.; Речна мрена (Барбус барбус) као биоиндикатор загађења речних седимената металима; Речна мрена (Барбус барбус) као биоиндикатор загађења речних седимената металима;
[ "https://nardus.mpn.gov.rs/bitstream/id/1184/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1185/IzvestajKomisije8314.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1184/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1185/IzvestajKomisije8314.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_7948" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/5477
123456789-5477.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:11197/bdef:Content/download
no
Између медија и инструмента: савремена феноменологија архитеконског модела
Contemporary Phenomenology of Architectural Model.
[ "Mako, Vladimir" ]
[ "Timotijević, Mihailo", "Pavić, Branko", "Šuvaković, Miodrag" ]
Stratimirović, Tatjana V.
2016-06-19T15:45:35
[ "2016-06-19T15:45:35", "2020-07-02T16:25:03" ]
2015-12-09
[ "https://nardus.mpn.gov.rs/handle/123456789/5477", "http://eteze.bg.ac.rs/application/showtheses?thesesId=2950", "https://fedorabg.bg.ac.rs/fedora/get/o:11197/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=513052561" ]
[ "Ова докторска дисертација испитује како се данас испољава појавност архитектонског модела, кроз приступ феноменолошке анализе. Намера тезе је двојака: да осветли улогу и значај модела за пројектовање савремене архитектуре, и да истовремено покуша да разјасни потребу за уважавањем феноменологије у домену савременог архитектонског образовања. Да би се ово спровело, рад је усредсређен на (1) питање савремености, (2) однос феноменологије и архитектуре и (3) анализирање предмета архитектонског модела. Теоријски план подупрт је експерименталним пројектима модела, коришћеним као архитектонско средство за истраживање аутономије идентитета, порекла форме и смисла сврхе, израђеним кроз студентске вежбе на Архитектонском факултету у Београду. Ова теза такође одражава и како се све могу употребљавати модели у архитектури." ]
[ "This doctoral thesis explores the contemporary appearance of phenomenon of architectural model, accessed through phenomenological analysis. The intention is twofold: to highlight the role and importance of models for the design of contemporary architecture, and at the same time to clarify the need for recognition of phenomenology in the field of contemporary architectural education. To carry out these intentions, the work focuses on (1) the issue of contemporariness, (2) the open relationship of phenomenology and architecture and (3) the structural analysis of the object of architectural model. Theoretical plane is underpinned by experimental project-models, used as architecture’s investigative tool for the study of autonomy identity, origin of form and meaning of purpose, delivered through student exercises at Belgrade Faculty of Architecture. This thesis also presents reflections on how to use models in architecture." ]
application/pdf
sr
Универзитет у Београду, Архитектонски факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]
Универзитет у Београду
[ "архитектонски модел", "савременост", "феноменологија", "медиј", "инструмент" ]
[ "architectural model", "contemporariness", "phenomenology", "medium", "instrument" ]
[ "Између медија и инструмента: савремена феноменологија архитеконског модела", "Contemporary Phenomenology of Architectural Model." ]
[ "" ]
doctoralThesis
[ "BY-NC-SA" ]
Мако, Владимир; Павић, Бранко; Шуваковић, Миодраг; Тимотијевић, Михаило; Стратимировић, Татјана В.; Između medija i instrumenta: savremena fenomenologija arhitekonskog modela;
[ "https://nardus.mpn.gov.rs/bitstream/id/907/Disertacija2828.pdf", "https://nardus.mpn.gov.rs/bitstream/id/908/Tatjana_Stratimirovic_Ocena_Referat_AF.pdf", "http://nardus.mpn.gov.rs/bitstream/id/907/Disertacija2828.pdf", "http://nardus.mpn.gov.rs/bitstream/id/908/Tatjana_Stratimirovic_Ocena_Referat_AF.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_5477" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/5559
123456789-5559.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:11228/bdef:Content/download
no
Концепт интегрисане рекреације и могућности примене у условима развоја градова Србије
The concept of integrated recreation and possibilities of implementation in development of Serbian cities
[ "Nikezić, Zoran" ]
[ "Đokić, Vladan", "Vujović, Sreten" ]
Živković, Jelena A.
2016-06-25T19:15:40
[ "2016-06-25T19:15:40", "2020-07-02T16:24:46" ]
2015-07-07
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=2994", "https://nardus.mpn.gov.rs/handle/123456789/5559", "https://fedorabg.bg.ac.rs/fedora/get/o:11228/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=47624207" ]
[ "Ова дисертација се бави истраживањем савременог развоја рекреације и њене улоге у урбаном развоју. У контексту трагања за новим обрасцима развоја, којима би се омогућило превазилажење проблема дезинтеграције урбане структуре, рекреација се преиспитује као интегративни урбани садржај који је као такав препознат у урбаној теорији. Фокус рада је на урбаној рекреацији, дакле оној која се одвија у граду и одражава урбане стилове живота. Пажња је усмерена на истраживање феномена \"интегрисане рекреације\" за који се претпоставља да значајно увећава могућности за рекреацију у савременом граду. Овај феномен је резултат укључивања рекреације у различите процесе производње простора. Реч је о процесима који, поред традиционалних нормативно-дистрибутивних политика развоја рекреације у јавном сектору, реализују рекреативне садржаје и просторе у савременом граду као средство развоја града. Циљ овог истраживања је био да утврди основе и испита могућности коришћења рекреације као средства повезивања урбане структуре, као и да омогући примену стечених знања у усмеравању развоја градова Србије. Будући да феномен интегрисане рекреације није укључен у постојећи концепт развоја рекреације у Србији, претпоставка о промени улоге рекреације у развоју савремених градова је условила да се, у складу са налазима истраживања, редефинише и концепт рекреације у урбаном развоју у Србији. Због тога је и истраживање било подељено у два дела. У првој фази су објашњене околности појаве феномена интегрисане рекреације, систематизоване карактеристике савремене провизије садржаја и простора рекреације, и на основу теоријског дефинисања рекреације као интегративног урбаног садржаја испитиване могућности коришћења рекреације као средства повезивања урбане структуре. Испитивање карактеристика интегрисане рекреације и њеног интегративног потенцијала у савременом граду било је засновано на жељи да се препознају..." ]
[ "This thesis explores the contemporary urban recreation and its use as a tool for urban development. Based on integrative capacity of recreation as an urban activity, the aim of the research was to examine possibilities to use this asset in development of Serbian cities. Since the existing concept of recreation development in Serbia doesn‟t acknowledge its possible use as a tool for urban development, thesis aims to redefine the concept of recreation so that it can be implemented in Serbian context. For that reason the research was organized on two levels. At the first level the integrative role of recreation was examined through research in urban theory and in the fields of psychology, sociology and geography of leisure. Integrative capacity of urban recreation was defined through concepts of leisure experience, encounter and practice and as such operationalized as fluid, focal and porous integrative practice. Different possibilities for activating integrative potential of urban recreation were identified for autonomous and integrated forms of urban recreation. The occurrence and spreading of the phenomena of integrated recreation is then explained and its integrative capacity explored through comparative analysis of eight concepts of development through recreation: „city of fun“, „fantasy city, „tourist city“, „experience city“, „city of play“, „healthy/active city“, „green city“ and „creative city“. The aim was to identify possibilities and constraints of each mode of urban development. The characteristics of each concept of development through recreation were mapped on hypothetical model of contemporary city so that a new „landscape of integrated recreation“ can be identified. The results have proved that various developent paths enabled recreation to function as an integrative tool for urban development at different levels, in various forms and on many locations in the city. These results formed the basis for redefining a concept of recreation in Serbia and for developing a model for integrating urban structure through development of..." ]
application/pdf
sr
Универзитет у Београду, Архитектонски факултет
[ "info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/36035/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "рекреација", "развој града", "урбана структура", "процеси производње простора", "интеграција", "Србија", "Београд" ]
[ "urban recreation", "leisure", "city development", "urban structure", "integration", "production of space", "Serbia", "Belgrade" ]
[ "Концепт интегрисане рекреације и могућности примене у условима развоја градова Србије", "The concept of integrated recreation and possibilities of implementation in development of Serbian cities" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Никезић, Зоран; Ђокић, Владан; Вујовић, Сретен; Живковић, Јелена A.; Koncept integrisane reakcije i mogućnosti primene u uslovima razvoja gradova Srbije;
[ "https://nardus.mpn.gov.rs/bitstream/id/861/Jelena_Zivkovic_Ocena_Referat_AF.pdf", "http://nardus.mpn.gov.rs/bitstream/id/860/Disertacija3522.pdf", "http://nardus.mpn.gov.rs/bitstream/id/861/Jelena_Zivkovic_Ocena_Referat_AF.pdf", "https://nardus.mpn.gov.rs/bitstream/id/860/Disertacija3522.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_5559" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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[ "" ]
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[ "" ]
[ "" ]
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[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/8939
123456789-8939.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:16799/bdef:Content/download
no
Geometrijsko-morfometrijske analize glavenog skeleta šakala (Canis aureus) i lisice (Vulpes vulpes) sa područja Srbije: biogeografski aspekti morfološke varijabilnosti
Geometric-morphometric analyses of golden jackal (Canis aureus) and red fox (Vulpes vulpes) skulls from the territory of Serbia: biogeographical aspects of morphological variability
[ "Ćirović, Duško" ]
[ "Jojić, Vida", "Penezić, Aleksandra", "Ćirović, Duško", "Jojić, Vida" ]
Porobić, Jelena M.
2017-12-12T15:45:02
[ "2017-12-12T15:45:02", "2020-07-03T08:06:02" ]
2017-09-24
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=5423", "https://nardus.mpn.gov.rs/handle/123456789/8939", "https://fedorabg.bg.ac.rs/fedora/get/o:16799/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025175474" ]
[ "Metodama geometrijske morfometrije, analizirana je varijabilnost veličine i oblika ventralnog, dorzalnog i lateralnog kranijuma i mandibule subadultnih i adultnih šakala (Canis aureus) i lisice (Vulpes vulpes) iz tri biogeografske oblasti sa područja Srbije: panonske, planinsko-kotlinske i peripanonske. Kod obe uzrasne grupe, utvrđeno je odsustvo razlika u veličini i prisustvo razlika u obliku glavenog skeleta između šakala iz različitih biogeografskih oblasti. Na većoj geografskoj skali ventralni, a na manjoj lateralni kranijum se pokazao kao najinformativniji. Između lisica iz tri biogeografske oblasti nema razlika u veličini, ali postoje razlike u obliku glavenog skeleta, sa lateralnim kranijumom kao najinformativnijim. Oblik glavenog skeleta lisica varira duž gradijenata geografske širine i dužine, kao i u zavisnosti od udela agrarnih površina, za razliku od njegove veličine koja je uniformna. Promene oblika ventralnog i lateralnog kranijuma uslovljene razlikama u udelu agrarnih površina su u saglasnosti sa promenama oblika duž gradijenata geografske širine i dužine, kao i sa biogeografskim obrascima varijabilnosti oblika. Kod obe vrste, statička alometrija ne utiče na biogeografske obrasce varijabilnosti oblika glavenog skeleta. Polni dimorfizam u veličini glavenog skeleta je izraženiji kod lisica u odnosu na šakale. Kod šakala, polni dimorfizam u obliku najuočljiviji je na nivou ventralnog kranijuma i mandibule. Kod lisica, polni dimorfizam u obliku, detektovan na nivou svih kranijalnih perspektiva, najuočljiviji je na nivou lateralnog kranijuma. Kod obe vrste, razlike između polova u obliku baze lobanje su uslovljene razlikama u veličini, dok širi rostralni region i zigomatični lukovi kod mužjaka nisu u vezi sa promenama u veličini." ]
[ "Geometric morphometric approaches were employed to explore size and shape changes of ventral, dorsal and lateral cranium and mandible in subadults and adults of golden jackal (Canis aureus) and red fox (Vulpes vulpes) from three biogeographical areas in Serbia: pannonian, mountain-valley and peripannonian. In both age groups, absence of size, but presence of cranial and mandibular shape differences were found between jackals from different biogeographical areas. At larger geographic scale ventral cranium was the most informative, while at smaller geographic scale it was lateral cranium. There were no skull size differences among foxes from three biogeographical areas, whereas shape changes were detected, and lateral cranium was the most informative. While uniform considering size, the shape of red fox skulls varies in relation to latitude and longitude, as well as depending on proportion of agricultural habitats. Shape changes of ventral and lateral cranium related to the proportion of agricultural habitats correspond to those associated with latitudinal and longitudinal gradients resembling biogeographical patterns of shape changes. Static allometry does not influence biogeographical patterns of shape changes observed for both species. Skull size differences between the sexes were more pronounced in foxes than in jackals. In jackals, sexual shape dimorphism was the most obvious at the level of ventral cranium and mandible. In foxes, sexual dimorphism was also detected for cranial shape, and lateral cranium was the most informative. In both species, basicranial shape changes between the sexes are size-related, while the wider rostral and zygomatic regions in males are not associated with size differences." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Canidae", "Canis aureus", "Vulpes vulpes", "geometrijska morfometrija", "biogeografska oblast", "veličina", "oblik", "glaveni skelet", "polni dimorfizam", "alometrija" ]
[ "Canidae", "Canis aureus", "Vulpes vulpes", "geometric morphometrics", "biogeographical area", "size", "shape", "skull", "sexual dimorphism", "allometry" ]
[ "Geometrijsko-morfometrijske analize glavenog skeleta šakala (Canis aureus) i lisice (Vulpes vulpes) sa područja Srbije: biogeografski aspekti morfološke varijabilnosti" ]
[ "Geometric-morphometric analyses of golden jackal (Canis aureus) and red fox (Vulpes vulpes) skulls from the territory of Serbia: biogeographical aspects of morphological variability" ]
doctoralThesis
[ "BY-NC-ND" ]
Ћировић, Душко; Јојић, Вида; Пенезић, Aлександра; Ћировић, Душко; Јојић, Вида; Поробић, Јелена М.; Геометријско-морфометријске анализе главеног скелета шакала (Цанис ауреус) и лисице (Вулпес вулпес) са подручја Србије: биогеографски аспекти морфолошке варијабилности; Геометријско-морфометријске анализе главеног скелета шакала (Цанис ауреус) и лисице (Вулпес вулпес) са подручја Србије: биогеографски аспекти морфолошке варијабилности;
[ "http://nardus.mpn.gov.rs/bitstream/id/1247/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1248/IzvestajKomisije15287.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1247/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1248/IzvestajKomisije15287.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_8939" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/10620
123456789-10620.pdf
no
no
https://fedorabg.bg.ac.rs/fedora/get/o:19186/bdef:Content/download
no
Morphological and molecular characterization of Aphidius eadyi species complex (Hymenoptera, Braconidae, Aphidiinae), parasitoids of pea aphid - Acyrthosiphon pisum Harr. (Hemiptera, Aphididae)
Морфолошка и молекуларна карактеризација врста Aphidius eadyi комплекса (Hymenoptera, Braconidae, Aphidiinae), паразитоида зелене луцеркине ваши - Acyrthosiphon pisum Harr. (Hemiptera, Aphididae)
[ "Tomanović, Željko" ]
[ "Petrović, Anđeljko", "Mitrović, Milana" ]
Ghaliow, Mustafa
2019-01-18T09:36:09
[ "2019-01-18T09:36:09", "2020-07-03T08:05:53" ]
2018-11-14
[ "https://nardus.mpn.gov.rs/handle/123456789/10620", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6461", "https://fedorabg.bg.ac.rs/fedora/get/o:19186/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025212594" ]
[ "" ]
[ "Acyrthosiphon pisum Harris is an aphid species of the greatest agricultural importance. It is a major pest on several plants of the family Fabacae, and there have been numerous programs involving biological control of Acyrthosiphon pisum worldwide. Species belonging to the Aphidius eadyi group have been used as biocontrol agents in those programs, but knowledge about their taxonomy and distribution has remained scarce with big gaps. Here we identify all aphidiine parasitoid species that have parasitized A. pisum in Europe, including three species within the Aphidius eadyi species group, using both molecular (mtDNA COI sequences) and morphological analyses. The Aphidius eadyi species group consists of the following species: Aphidius smithi, A. eadyi, and A. banksae. Morphological characterization showed that the most important morphological characters for separation of species of the Aphidius eadyi group are: shape of costulae on the anterolateral part of the petiole; shape of the central areola on the propodeum; and shape and venation of the forewings. Forewing shape was analysed using geometric morphometrics, and it is demonstrated that all three species differ in wing shape with some overlap. Morphological differences were confirmed by molecular data, mean genetic distances between the species varying from 5 to 7.4%. Identification of Aphidius banksae as a widely distributed pea aphid parasitoid whose range covers most of the western Palaearctic (from the United Kingdom to Israel) is the most interesting finding of this study. In addition, Aphidius banksae is diagnosed and redescribed. A key for identification of all aphidiine species attacking Acyrthosiphon pisum in Europe is provided.", "Acyrthosiphon pisum Harris је једна од економски најзначајнијих биљних ваши у пољопривреди, првенствено на културама из фамилије Fabacae. Из тог разлога су и бројни програми биолошке контроле реализовани широм света. У овим програмима су веома често као агенти коришћене врсте Aphidius eadyi комплекса. И поред честе употребе и значаја, број врста унутар комплекса, њихова таксономија и распрострањење су углавном непознати. У овој студији извршена је идентификација свих паразитоида из потфамилије Aphidiinae који паразитирају A. pisum на простору Европе. Међу њима су, употребом морфолошких и молекуларних анализа (секвенци mtCOI гена) идентификоване три врсте које припадају Aphidius eadyi комплексу: Aphidius smithi, A. eadyi и A. banksae. Морфолошком карактеризацијом је утврђено да су за разликовање ових врста најзначајнији следећи морфолошки карактери: облик бразди на антеролатералном региону петиолуса, облик централне ареоле на проподеуму, облик и нерватура предњих крила. Облик предњих крила је анализиран употребом геометријске морфометрије и утврђено је да се све три врсте разликују и поред мањег преклапања. Морфолошке разлике су потврђене и молекуларним анализама којима је утврђено да се генетичке дистанце између врста A. eadyi комплекса крећу у распону од 5% до 7,4%. Идентификација врсте Aphidius banksae, као широко распрострањеног паразитоида зелене луцеркине ваши представља најинтересантнији налаз ове студије. Утврђено је да распрострањење врсте A. banksae обухвата највећи део западног Палеарктика, од Уједињеног Краљевства до Израела. Додатно, дат је и поновни опис врсте Aphidius banksae као и кључ за идентификацију свих паразитоида потфамилије Aphidiinae који паразитирају Acyrthosiphon pisum у Европи." ]
application/pdf
en
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Aphidius eadyi species complex", "mtDNA barcoding", "geometric morphometrics", "integrative taxonomy" ]
[ "Aphidius eadyi коплекс врста", "ДНК баркодинг", "геометријска морфометрија", "интегративна таксономија" ]
[ "Morphological and molecular characterization of Aphidius eadyi species complex (Hymenoptera, Braconidae, Aphidiinae), parasitoids of pea aphid - Acyrthosiphon pisum Harr. (Hemiptera, Aphididae)" ]
[ "Морфолошка и молекуларна карактеризација врста Aphidius eadyi комплекса (Hymenoptera, Braconidae, Aphidiinae), паразитоида зелене луцеркине ваши - Acyrthosiphon pisum Harr. (Hemiptera, Aphididae)" ]
doctoralThesis
[ "BY-NC-ND" ]
[ "http://nardus.mpn.gov.rs/bitstream/id/1199/IzvestajKomisije18777.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1199/IzvestajKomisije18777.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1198/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1198/Disertacija.pdf", "https://fedorabg.bg.ac.rs/fedora/get/o:19186/bdef:Content/download" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10620" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/7943
123456789-7943.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:15156/bdef:Content/download
no
Имуномодулаторни ефекти антикоагуланта варфарина код пацова
Immunomodulatory effects of anticoagulant warfarin in rats
[ "Pejović, Janko" ]
[ "Kataranovski, Milena", "Kataranovski, Milena", "Kataranovski, Dragan", "Vasilijić, Saša" ]
Subota, Vesna S.
2017-04-22T09:33:44
[ "2017-04-22T09:33:44", "2020-07-03T08:06:20" ]
2016-09-28
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=4828", "https://nardus.mpn.gov.rs/handle/123456789/7943", "https://fedorabg.bg.ac.rs/fedora/get/o:15156/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025143218" ]
[ "Варфарин (4-хидроксикумарин) је антагонист витамина К. Овај кумарински дериват се користи као антикоагулантни родентицид и као терапеутско средство у профилакси тромбоемболијских болести. Антикоагулантни ефекат варфарина се заснива на инхибицији корака, који зависе од витамина К, у синтези бројних фактора коагулације у јетри. Захваљујући инхибицији циклуса витамина К, варфарин утиче и на друге протеине неопходне за биолошке процесе ван хемостазе (раст и калцификацију костију, раст глаткомишићних ћелија васкулатуре и мезангијских ћелија и друге), доводећи до штетних последица. Варфарин инхибира и процесе који нису у вези са витамином К, укључујући раст тумора. Малобројни подаци указују да овај агенс може да делује и на поједине аспекте имунске функције. Механизми деловања варфарина на имунски систем су, међутим, најмање познати. Ова дисертација је имала за циљ да испита имуномодулаторни потенцијал варфарина након његове епикутане и оралне примене код пацова. Акутни епикутани третман (три дана за редом у дози од 10 μg и 100 μg натријум варфарина) одговара професионалној или акциденталној изложености, а субакутни орални третман (30 дана у дози од 0.35 mg/L и 3.5 mg/L натријум варфарина у пијаћој води) одговара терапијској примени. Проинфламаторни и имуномодулаторни потенцијал варфарина је испитан анализом хуморалних параметара (концентрација акутно фазних протеина и проинфламаторног цитокина, интерлеукина 6 (IL-6) у плазми, као и активности основних ензима антиоксидативне одбране супероксид дисмутазе и каталазе у еритроцитима), као и ћелијских параметара [квантитативне и квалитативне промене леукоцита периферне крви, посебно полиморфонуклеарних леукоцита (PMN)] као показатеља запаљења на системском нивоу. Механизми дејства на полиморфонуклеарне ћелије периферне крви испитани су у погледу њихове оксидативне активности, продукције проинфламаторних цитокина, фактора некрозе тумора (TNF) и IL-6, способности адхезије, миграције и екстравазације у циљна ткива, коришћењем модела субкутано имплантираних стерилних поливинилских сунђера. Резултати су показали да варфарин доспева у организам и када се наноси на кожу и након оралне примене, судећи по продужењу протромбинског времена..." ]
[ "Warfarin (4-hydroxycoumarin) is a vitamin K antagonist. This coumarin derivative is used as an anticoagulant rodenticide and as a therapeutic agent for the prophylaxis of thromboembolic disorders. The anticoagulant effect of warfarin is based on the inhibition of the steps which are dependent on Vitamin K in the synthesis of a number of coagulation factors in the liver. Owing to the inhibition of the vitamin K cycle, warfarin also affects the other proteins which are necessary for the biological processes apart from hemostasis (growth and calcification of bones, vascular smooth muscle cells, mesangial cells and others), leading to harmful effects. Warfarin also inhibits the processes which are not related to vitamin K, including the growth of tumors. Few data suggest that this agent may act on certain aspects of immune function. Mechanisms of action of warfarin on the immune system are, however, the least known. This study was aimed to investigate the immunomodulatory potential of warfarin after its epicutaneous and oral administration in rats. The acute epicutaneous treatment (within three consecutive days in doses of 10 μg and 100 μg sodium warfarin) corresponds to professional or accidental exposure, and the subacute oral treatment (30 days in doses of 0.35 mg/L and 3.5 mg/L sodium warfarin in drinking water) corresponds to the therapeutic use. The proinflammatory and immunomodulatory potential of warfarin has been tested by analyzing the humoral parameters (concentration of acute phase proteins and pro-inflammatory cytokines, interleukin 6 (IL-6) in the plasma, as well as the activities of basic enzymes of antioxidant defence, superoxide dismutase and catalase in erythrocytes) as well as the cell parameters [quantitative and qualitative changes in peripheral blood leukocytes, especially polymorphonuclear leukocytes (PMN)] as an indicator of inflammation at the system level. Mechanisms of effects to the peripheral blood polymorphonuclear cells were tested with regard to their oxidative activity, production of pro-inflammatory cytokines, tumor necrosis factor (TNF) and IL-6, ability of adhesion, migration and extravasation in the target tissues, using models of subcutaneously implanted sterile polyvinyl sponges. The results showed that warfarin comes into the organism both when it is applied on the skin and after oral administration, judging by the extension of prothrombin time..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Лабораторијски пацови", "епикутана апликација варфарина", "орални субакутни режим примене варфарина", "субкутани стерилни поливинилски сунђери", "полиморфонуклеарни леукоцит (PMN)", "инфламација" ]
[ "Laboratory rats", "epicutaneous application of warfarin", "oral subchronic administration regimen of warfarin", "subcutaneous sterile polyvinyl sponges", "polymorphonuclear leukocyte (PMN)", "inflammation" ]
[ "Имуномодулаторни ефекти антикоагуланта варфарина код пацова" ]
[ "Immunomodulatory effects of anticoagulant warfarin in rats" ]
doctoralThesis
[ "BY-NC-ND" ]
Пејовић, Јанко; Катарановски, Милена; Василијић, Саша; Катарановски, Драган; Катарановски, Милена; Субота, Весна С.; Imunomodulatorni efekti antikoagulanta varfarina kod pacova;
[ "https://nardus.mpn.gov.rs/bitstream/id/1331/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1332/IzvestajKomisije8308.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1331/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1332/IzvestajKomisije8308.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_7943" ]
[ "" ]
[ "" ]
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[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/9382
123456789-9382.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:17528/bdef:Content/download
no
Потенцијал рекомбинантних протеина пљувачке крпеља Ixodes ricinus (Linnaeus, 1758) за детекцију специфичних антитела као маркера убода крпељa
Potential of recombinant Ixodes ricinus (Linnaeus, 1758) tick saliva proteins for detection of specific antibodies as markers of tick bite
[ "Marković, Dragana" ]
[ "Božić Nedeljković, Biljana", "Radulović, Željko", "Tomanović, Snežana", "Stanisavljević, Ljubiša Ž." ]
Mihaljica, Darko B.
2018-04-23T09:30:08
[ "2018-04-23T09:30:08", "2020-07-03T08:06:09" ]
2017-12-27
[ "https://nardus.mpn.gov.rs/handle/123456789/9382", "http://eteze.bg.ac.rs/application/showtheses?thesesId=5722", "https://fedorabg.bg.ac.rs/fedora/get/o:17528/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025183410" ]
[ "Процена изложености крпељима је значајна у епидемиолошким студијама обољења чије узрочнике преносе крпељи, а потврда убода крпеља је важна за њихову дијагностику. У овом раду су испитана три протеина пљувачке, PA107, AV422 и калретикулин (CAL), крпеља Ixodes ricinus, медицински најзначајније врсте у Европи, у контексту употребе за детекцију специфичних антитела у серуму домаћина, као маркера убода. Анализа варијабилности њихових секвенци је указала на високу конзервираност сва три протеина, при чему се PA107 показао као најконзервиранији. С обзиром на убиквитарну заступљеност CAL код еукариота, даљи фокус у овој студији је био на PA107 и AV422, као протеинима који су специфични за крпеље и чији хомолози су први пут анализирани код врсте Ixodes ricinus. Испитивана је реактивност серума експерименталних животиња, пацова, на којима су храњене ларве Ixodes ricinus или Dermacentor reticulatus, са рекомбинантним формама IrPA107 и IrAV422. Коришћењем rIrAV422, специфична антитела су детектована код свих инфестираних пацова, што указује да се rIrAV422 може употребити за детекцију маркера убода различитих група тврдих крпеља уз високу сензитивност и специфичност. Реактивност у случају IrPA107 је била значајно мања. За испитивање употребе rIrAV422 у детекцији маркера убода у серумима домаћина изложених крпељима у природном окружењу, коришћени су ловачки пси као адекватна индикаторска група. Добијена серореактивност код свих анализираних паса у студији указује на широк потенцијал примене овог протеина пљувачке у процени изложености различитим врстама крпеља и ризика од инфекције патогенима које они преносе." ]
[ "Assessment of exposure to ticks is a significant data in epidemiological studies of tick-borne diseases, and confirmation of tick bite is important for their diagnosis. In this study, three saliva proteins, PA107, AV422 and calreticulin (CAL), of Ixodes ricinus tick, medically most important species in Europe, were examined in the context of their use for detection of specific antibodies in sera of hosts, as bite markers. Sequence variability analysis revealed high conservativity of all three proteins, with PA107 as the most conservative. Since CAL is ubiquitous in eukaryotes, the further focus of this study was on PA107 and AV422, as proteins that are specific for ticks. Homologues of these proteins from Ixodes ricinus were analyzed for the first time. Reactivity of sera of experimental animals, rats, which were infested separately with Ixodes ricinus or Dermacentor reticulatus larvae, with recombinant forms of IrPA107 and IrAV422 were examined. Specific antibodies were detected in sera of all infested rats using rIrAV422, which implied its usage for detection of bites of tick species that belong to different groups of ixodid ticks, with high sensitivity and specificity. Reactivity with IrPA107 was significantly lower. Hunting dogs, as good sentinels, were used for assessment of rIrAV422 usage for detection of markers in sera of hosts which are exposed to ticks in natural environment. Obtained seroreactivity in all dogs implies broad applicability of this saliva protein, in terms of assessment of exposure to different tick species and risk of infection with tick-borne pathogens." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173006/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "протеини пљувачке", "рекомбинантни протеини", "антитела", "маркери убода крпеља", "изложеност крпељима" ]
[ "saliva proteins", "recombinant proteins", "antibodies", "tick bite markers", "exposure to ticks" ]
[ "Потенцијал рекомбинантних протеина пљувачке крпеља Ixodes ricinus (Linnaeus, 1758) за детекцију специфичних антитела као маркера убода крпељa" ]
[ "Potential of recombinant Ixodes ricinus (Linnaeus, 1758) tick saliva proteins for detection of specific antibodies as markers of tick bite" ]
doctoralThesis
[ "BY-NC-ND" ]
Марковић, Драгана; Радуловић, Жељко; Станисављевић, Љубиша Ж.; Томановић, Снежана; Божић Недељковић, Биљана; Михаљица, Дарко Б.; Potencijal rekombinantnih proteina pljuvačke krpelja Ixodes ricinus (Linnaeus, 1758) za detekciju specifičnih antitela kao markera uboda krpelja;
[ "http://nardus.mpn.gov.rs/bitstream/id/1290/IzvestajKomisije16025.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1290/IzvestajKomisije16025.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1289/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1289/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_9382" ]
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https://nardus.mpn.gov.rs/handle/123456789/9548
123456789-9548.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:17746/bdef:Content/download
no
Diverzitet i uloga mikromiceta u procesu biodeterioracije zidnih slika crkve Svetog Vaznesenja Gospodnjeg u Velikom Krčimiru
Diversity and role of micromycetes in wall painting biodeterioration process: Church of the Holy Ascension in Veliki Krčimir
[ "Ljaljević Grbić, Milica" ]
[ "Vukojević, Jelena", "Đorđević, Aleksandar", "Janaćković, Peđa", "Debljović Ristić, Nevena" ]
Unković, Nikola D.
2018-07-02T11:36:10
[ "2018-07-02T11:36:10", "2020-07-03T08:05:55" ]
2018-04-20
[ "https://nardus.mpn.gov.rs/handle/123456789/9548", "http://eteze.bg.ac.rs/application/showtheses?thesesId=5844", "https://fedorabg.bg.ac.rs/fedora/get/o:17746/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50177807" ]
[ "Analiziran je diverzitet i sezonska dinamika mikromiceta na zidnim slikama i u vazduhu istraživane crkve. Identifikovana je mikobiota od 46 taksona mikromiceta, sa dominacijom Aspergillus, Penicillium i Cladosporium vrsta. Najveći diverzitet je zabeležen tokom leta, dok je najveća brojnost propagula po jedinici površine (“fungalni otisak”) konstatovana u zimu. Aeromikobiota je okarakterisana sa 33 taksona gljiva. Kontaminacija vazduha propagulama gljiva tokom godine višestruko je prevazilazila standarde za zatvorene prostore. Mikroskopska analiza biofilma pokazala je da su reproduktivne strukture Cladosporium sp. i Chaetomium sp. u kontaktu bojenog sloja i maltera glavni biotski faktor deterioracije. Metodom ATP bioluminiscenije, 75% površina zidnih slika je okarakterisano kao “Zona opasnosti”. Monitoringom indukovane i spontane kolonizacije na modelu zidne slike zaključeno je da su lihenizovane i mikrokolonijalne gljive uzrok fenomena “biopitting”. Testirani izolati su demonstrirali veliki potencijal deterioracije zidnih slika u eksperimentima in vitro. BAC i novosintetisani BAC/FNP nanokompozit pokazali su dobru antifungalnu aktivnost, kao i uticaj na povećanje produkcije aflatoksina B1 i smanjenje produkcije ohratoksina A. Etarsko ulje tamjana imalo je jači antifungalni efekat u odnosu na etarsko ulje smirne, ali slabiji u poređenju sa smešom ulja. Dim tamjana poseduje inhibitorno dejstvo na germinaciju konidija, mehaničkim i hemijskim dejstvom voštanog sloja deponovanog iz dima, in vitro. Tretman vazduha crkve dimom tamjana redukovao je nivo kontaminacije vazduha fungalnim propagulama za približno 80%. Preko predloženog metodološkog protokola, data je mogućnost implementacije rezultata ovog istraživanja u praksu konzervacije i restauracije kulturnih dobara." ]
[ "Diversity and seasonal dynamics of micromycetes from wall paintings and surrounding air of investigated church was analyzed. A total of 46 fungal taxa was documented, with dominance of Aspergillus, Penicillium and Cladosporium species. The greatest species diversity was recorded in the summer, while the highest abundance of fungal propagules per unit area (“fungal print”) was observed in the winter. Air mycobiota was characterized by 33 fungal taxa. Fungal air contamination, throughout the year, exceeded permisable levels for indoor environment. Microscopic biofilm analyzes revealed that reproductive structures of Cladosporium sp. and Chaetomium sp. are the main biodeteriogens. ATP bioluminescence method showed that 75% of the surfaces are deemed as “Danger zone”. Monitoring of induced and spontaneous colonization of mock-model established lichenized and micro-colonial fungi as the main causative agents of “biopitting” phenomenon. Tested isolates demonstrated pronounced deterioration capabilities in experiments in vitro. BAC and newlysynthesized BAC/FNP nanocomposite had very strong antifungal activity, stimulated aflatoxin B1 production, and inhibited ochratoxin A production. Frankincense essential oil showed stronger antifungal property compared to the oil obtained from myrrh, but weaker than the oil mixture. Burn incense had inhibitory effect on conidia germination via mechanical and chemical activity of thin waxy layer deposited from fume, in vitro. Treatment of air within church, with burn incense, reduced the level of fungal air contamination by approximatly 80%. Through the proposed methodological protocol, the possibility of implementing results into the practice of conservation and restoration of cultural heritage is given." ]
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Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173032/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "antifungalna aktivnost", "biodeterioracija", "biofilm", "“fungalni otisak”", "mikromicete", "model zidne slike", "nanokompozit", "tamjan", "Veliki Krčimir", "zidne slike" ]
[ "antifungal activity", "biodeterioration", "biofilm", "frankincense", "“fungal print”", "micromycetes", "mock-model", "nanocomposite", "Veliki Krčimir", "wall paintings" ]
[ "Diverzitet i uloga mikromiceta u procesu biodeterioracije zidnih slika crkve Svetog Vaznesenja Gospodnjeg u Velikom Krčimiru" ]
[ "Diversity and role of micromycetes in wall painting biodeterioration process: Church of the Holy Ascension in Veliki Krčimir" ]
doctoralThesis
[ "BY-NC-ND" ]
Љаљевић Грбић, Милица; Вукојевић, Јелена; Ђорђевић, Aлександар; Јанаћковић, Пеђа; Дебљовић Ристић, Невена; Унковић, Никола Д.; Диверзитет и улога микромицета у процесу биодетериорације зидних слика цркве Светог Вазнесења Господњег у Великом Крчимиру; Диверзитет и улога микромицета у процесу биодетериорације зидних слика цркве Светог Вазнесења Господњег у Великом Крчимиру;
[ "http://nardus.mpn.gov.rs/bitstream/id/1212/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1213/IzvestajKomisije17021.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1213/IzvestajKomisije17021.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1212/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_9548" ]
[ "" ]
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[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/9550
123456789-9550.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:17753/bdef:Content/download
no
Razvoj visoko automatizovanih mikrotitarskih testova na Saccharomyces cerevisiae za detekciju jedinjenja sa antifungalnim i citostatskim dejstvom
Development of two high-throughput screening methods to identify molecules with antifungal and cytostatic activity using Saccharomyces cerevisiae
[ "Vuković-Gačić, Branka" ]
[ "Knežević-Vukčević, Jelena", "Krnjaić, Dejan" ]
Petrović-Bulatović, Katarina
2018-07-02T11:36:11
[ "2018-07-02T11:36:11", "2020-07-03T08:05:59" ]
2018-03-30
[ "https://nardus.mpn.gov.rs/handle/123456789/9550", "http://eteze.bg.ac.rs/application/showtheses?thesesId=5850", "https://fedorabg.bg.ac.rs/fedora/get/o:17753/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50173199" ]
[ "Kvasac Saccharomyces cerevisiae je široko upotrebljavan model za istraživanje potencijala malih molekula u modulaciji signalnih puteva relevantnih za nastanak i razvoj bolesti u eukariotskim organizmima. U ovoj tezi je opisan razvoj dva visoko automatizovana testa zasnovana na praćenju ćelijske vijabilnosti kroz merenje fluorescentnog signala redukcijom alamarBlue® (resazurina) ili direktnim merenjem optičke gustine. Obe metode su pogodne za pretragu velikog broja uzoraka, u mikrotitarskim pločama sa 348 i 1536 bunarčića upotrebom diploidnog pdr5/pdr5 soja S.cereviseae kome nedostaje glavni transmembranski transporter Pdr5p, koji doprinosi multirezistenciji na lekove. Oba testa su pokazala visok kvalitet sa Z’ vrednosti > 0,5. Korišćenjem alamarBlue® testa ispitano je 1.101.408 jedinjenja male molekulske težine, od kojih je 25.144 inhibiralo rast kvasca. Određivanjem IC50 vrednosti, od 1930 odabranih jedinjenja iz primarnog testiranja, inhibitorni potencijal je potvrđen u 868 (45%) u oba ili bar jednom testu. Rezultat dobijeni haplodeficijentnim profilisanjem genoma kvasca su pokazali njihovu visoku raznovrsnost delovanja, 572 odbrana aktivna jedinjenja je uticalo na rast >25% ukupnog broja ispitivanih sojeva kvasca. Molekuli sa inhibitornom aktivnošću detektovanom na ovakav način predstavljaju impresivnu bazu za dalje istraživanje mehanizama putem kojih suprimiraju proliferaciju i/ili vijabilnost ćelija kvasaca, drugih patogenih gljiva, ali i sisarskih ćelija." ]
[ "The budding yeast S. cerevisiae is widely used model organism to elucidate the mechanism of action of low molecular weight compounds in modulation of signaling pathways involved in different diseases in eukaryotic organisms. This thesis describes the development of two high throughput screening methods based on cell viability either by monitoring the reduction of alamarBlue® (resazurin) or by direct optical measurement of cell growth. Both methods can be miniaturized to allow screening of large numbers of samples, in 384 and 1536-well format using the diploid pdr5/pdr5 strain of S.cereviseae lacking the major transmembrane multi-drug resistance pump Pdr5p. Both tests show high quality with Z’ value > 0.5. With the alamarBlue® approach, 1,101,408 low molecular weight compounds were screened, identifying 25,144 primary hits. Dose response curves (IC50) generated for a subset of 1930 active compounds using both alamarBlue® and optical density methods confirmed inhibitory potential for 868 of them either by both or one method. In genome-wide haploinsufficiency profiling (HIP), 572 of selected hits demonstrated a diverse mechanism of action, affecting >25% of all yeast strains. These hits represent an impressive basis for further evaluation of mechanisms of action for suppression of yeast’s, pathogenic fungi and mammalian cells proliferation and viability." ]
application/pdf
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Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]
Универзитет у Београду
[ "ultra visoko automatizovani testovi", "Saccharomyces cerevisiae", "alamarBlue®", "optička gustina" ]
[ "High-Throughput screening", "Saccharomyces cerevisiae", "alamarBlue®", "optical density" ]
[ "Razvoj visoko automatizovanih mikrotitarskih testova na Saccharomyces cerevisiae za detekciju jedinjenja sa antifungalnim i citostatskim dejstvom" ]
[ "Development of two high-throughput screening methods to identify molecules with antifungal and cytostatic activity using Saccharomyces cerevisiae" ]
doctoralThesis
[ "BY-NC-SA" ]
Вуковић-Гачић, Бранка; Кнежевић-Вукчевић, Јелена; Крњаић, Дејан; Петровић-Булатовић, Катарина;
[ "https://nardus.mpn.gov.rs/bitstream/id/1226/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1227/IzvestajKomisije17025.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1226/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1227/IzvestajKomisije17025.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_9550" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/10711
123456789-10711.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:19338/bdef:Content/download
no
Morfološka varijabilnost, evolucija i razviće kičmenice kod velikih mrmoljaka (Triturus, Salamandridae, Caudata)
Morphological variability, evolution and development of vertebral column in crested newts (Triturus, Salamandridae, Caudata)
[ "Ivanović, Ana" ]
[ "Urošević, Aleksandar", "Filipović, Branko", "Ivanović, Ana", "Urošević, Aleksandar" ]
Slijepčević, Maja
2019-01-31T12:45:45
[ "2019-01-31T12:45:45", "2020-07-03T08:06:06" ]
2018-05-18
[ "https://nardus.mpn.gov.rs/handle/123456789/10711", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6564", "https://fedorabg.bg.ac.rs/fedora/get/o:19338/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025211570" ]
[ "Analize intra- i interspecijske varijabilnosti u ontogeniji morfoloških struktura (npr. razlike u vremenu diferencijacije, stopi i pravcu ontogenetskih promena u veličini i obliku), kao i varijabilnosti na adultnom stupnju, omogućavaju utvrđivanje obrazaca morfološke varijabilnosti i sagledavanje procesa i mehanizma koji dovode do evolucionih promena morfoloških struktura. U ovoj tezi izučavana je morfološka varijabilnost osovinskog skeleta u okviru monofiletske grupe velikih mrmoljaka koju čine devet vrsta roda Triturus. Različit stepen izduživanja tela, varijabilnost u broju trupnih pršljenova kao i poznati filogenetski odnosi čine ovu grupu izuzetno pogodnim model-organizmima za studije razvića kičmenice i evolucije kičmenih pršljenova. Histološkim metodama praćeno je postembrionsko razviće trupnih pršljenova, radi utvrđivanja eventualnih razlika u vremenu formiranja, osifikacionim sekvencama kao i stopi njihove diferencijacije. Za ove analize odabrane su dve vrste velikih mrmoljaka koje se razlikuju u stepenu izduživanja tela, odnosno u broju trupnih pršljenova. Utvrđeno je da nema razlika u vremenu i načinu formiranja pršljenova između vrsta. Tokom formiranja kičmenih pršljenova zabeležena su tri tipa osifikacije: 1) perihordalna osifikacija, 2) perihondrijalna osifikacija i 3) endohondralna osifikacija. Takođe, zabeleženo je i prisustvo intravertebralne (notohordalne) hrskavice unutar tela pršljena pri čemu je razviće kičmenih pršljenova roda Triturus veoma slično razviću kod drugih vrsta repatih vodozemaca. Homeotske transformacije predstavljaju transformacije pri kojima jedan deo tela preuzima identitet drugog dela tela (npr. transformacija trupnog u sakralni pršljen) usled mutacije ili promena u ekspresiji određenih Hox gena. Studija variabilnosti u broju trupnih pršljenova i tipova homeotskih transformacija urađena je na uzorku koji pokriva celokopnu genetičku i geografsku varijabilnost roda Triturus. Uočeno je odsustvo korelacije između nepotpunih homeotskih transformacija sakralnog pršljena i varijabilnosti u broju trupnih pršljenova što može biti rezultat razvojnih mehanizama koji favorizuju kompletan broj presakralnih pršljenova ili favorizovanje selekcije protiv tranzicionih pršljenova kod ove grupe repatih vodozemaca..." ]
[ "Analyses of intra- and interspecific morphological variation through ontogeny (e.g. difference in the time of differentiation, rate and direction of ontogenetic changes in size and shape), as well as variability at the adult stage, enable determination of morphological patterns and mechanism which lead to evolution of morphological structures. In this thesis, morphological variability of axial skeleton was studied within monophyletic group of crested newts which comprises nine species of the genus Triturus. Crested newts are suitable model organisms for studies of vertebral development and evolution. They are closely related group of species with known phylogenetic relations, different rate of body elongation and great variability in the number of trunk vertebrae. Histological techiques were used in tracing postembrionic development of trunk verteberae to test difference in the time of vertebral development, ossification sequences and rate of vertebral differentiation. For these analyses, two species of crested newts which differ in rate of body elongation and number of trunk vertebrae were chosen. Results show that there is no difference between analysed species of Triturus newts in development time and formation of vertebrae. Three modes of the ossification process were recorded during vertebral formation: 1) perichordal ossification, 2) perichondrial ossification and 3) endochondral ossification. Also, the presence of intravertebral (notochordal) cartilage was recorded at centrum which makes vertebral development in Triturus newts very similar to other species of tailed amphibians. Homeotic transformations are replacements of one body part by another (e.g. transformation of trunk into sacral vertebra) due to the mutation or changes in expression of certain Hox genes. The studies of variability in the number of trunk vertebrae and types of homeotic transformations were done on sample that represents all genetical and geographical variability in the genus Triturus. The absence of correlation between the frequency of incomplete homeotic transformation of sacral vertebrae and variation in the number of trunk vertebrae could be a result of developmental mechanisms that favour complete numbers of presacral vertebrae or selection against transitional vertebrae in this group of tailed amphibians..." ]
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Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173043/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Triturus sp.", "kičmeni pršljen", "razviće", "tipovi osifikacije", "inter- i intravertebralna hrskavica", "homeotske transformacije", "broj pršljenova", "veličina", "oblik", "geometrijska morfometrija" ]
[ "Triturus sp.", "vertebra", "development", "modes of ossification", "inter- and inbtravertebral cartilage", "homeotic transformation", "vertebral number", "size", "shape", "geometric morphometric" ]
[ "Morfološka varijabilnost, evolucija i razviće kičmenice kod velikih mrmoljaka (Triturus, Salamandridae, Caudata)" ]
[ "Morphological variability, evolution and development of vertebral column in crested newts (Triturus, Salamandridae, Caudata)" ]
doctoralThesis
[ "BY-NC-ND" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1269/IzvestajKomisije18916.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1268/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1269/IzvestajKomisije18916.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1268/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10711" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/8938
123456789-8938.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:16798/bdef:Content/download
no
Analiza polimorfizama gena za receptor za vitamin D i gena asociranih sa trombofilijom kod žena sa idiopatskim infertilitetom
Analysis of the polymorphisms in vitamin D receptor gene and genes associated with thrombophilia in women with idiopathic infertility
[ "Stojković, Oliver" ]
[ "Zeljić, Katarina", "Stamenković, Gorana" ]
Đurović, Jelena Ž.
2017-12-12T15:45:01
[ "2017-12-12T15:45:01", "2020-07-03T08:06:01" ]
2017-10-02
[ "https://nardus.mpn.gov.rs/handle/123456789/8938", "http://eteze.bg.ac.rs/application/showtheses?thesesId=5421", "https://fedorabg.bg.ac.rs/fedora/get/o:16798/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025171890" ]
[ "Uvod. Genetičke analize mogu ukazati na uzrok infertiliteta kod ţena kod kojih klinički testovi nisu uspeli da utvrde razloge reproduktivnog neuspeha. Cilj. Cilj ovog rada je da se ispita postojanje veze genskih polimorfizama asociranih sa trombofilijom i polimorfizama u genu za receptor za vitamin D (VDR) sa idiopatskim infertilitetom. Materijal i metode. U studiju je uključeno 117 pacijentkinja sa idiopatskim infertilitetom, kao i 130 ţena sa najmanje jednom trudnoćom realizovanom bez komplikacija. Primenom TaqMan metode uzorci su genotipizirani u odnosu na polimorfizme: FV 1691 G>A, FII 20210 G>A, MTHFR 677 C>T, MTHFR 1298 A>C, PAI-1 -675 4G/5G, ATIII 786 G>A, ACE I/D i ITGB3 1565 T>C, dok su polimorfizmi u VDR genu analizirani primenom restrikcionih enzima (FokI, BsmI, ApaI i TaqI). Rezultati. Ispitivanjem etioloških faktora pokazano je da je porodična anamneza značajan faktor pri proceni individualnog rizika za infertilitet. Utvrđeno je da visokorizične varijante FV 1691A i FII 20210A predstavljaju nezavisne faktore rizika za nastanak primarnog infertiliteta. Analizom multilokusnih interakcija definisani su kompleksniji genotipovi asocirani sa sekundarnim infertilitetom. Ispitivanja polimorfizama u VDR genu pokazala su postojanje protektivne uloge alela F u FokI i alela B u BsmI polimorfizmu. Pored toga, analizom vezanosti markera u VDR genu identifikovano je postojanje haplotipova, od kojih je bAT povećavao rizik za sekundarni infertilitet, dok je BAT imao protektivnu ulogu za nastanak primarnog infertiliteta. Zaključak. Analiza polimorfizama u genima asociranim sa trombofilijom i genu za VDR ne samo da definiše moguće kliničko-genetičke dijagnostičke procedure, već sugeriše moguće mehanizme za odrţavanje hemostatskog i imunološkog balansa u procesu reprodukcije." ]
[ "Introduction. Genetic analysis may indicate cause of infertility in women to whom clinical trials have failed to determine the causes of reproductive failure. Aim. The aim of this study is to investigate a link between polymorphisms associated with thrombophilia and polymorphisms in the vitamin D receptor gene (VDR) with idiopathic infertility. Material and methods. This study included 117 female patients with idiopathic infertility, as well as 130 women with at least one pregnancy without complications. Using TaqMan method, the samples were genotyped for polymorphisms: FV 1691 G>A, FII 20210 G>A, MTHFR 677 C>T, MTHFR 1298 A>C, PAI-1 -675 4G/5G, ATIII 786 G>A, ACE I/D and ITGB3 1565 T>C, while VDR gene polymorphisms were analyzed using restriction enzymes (FokI, BsmI, ApaI and TaqI). Results. Examination of etiological factors has shown that family history is a significant factor in assessing individual risk for infertility. High risk variants FV 1691A and FII 20210A have been shown to be independent risk factors for occurrences of primary infertility. An analysis of multilocus interactions defined more complex genotypes associated with secondary infertility. Polymorphisms testing in VDR gene showed the existence of a protective role of allele F in FokI and allele B in BsmI polymorphisms. In addition, the analysis of the linkage in VDR gene identified the existence of haplotypes, of which bAT increased the risk of secondary infertility, while BAT had a protective role of primary infertility. Conclusions. The analysis of polymorphisms in the genes associated with thrombophilia and the VDR gene not only defines possible clinical-genetic diagnostic procedures, but suggests possible mechanisms for maintaining hemostatic and immune balance in the reproductive process." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "idiopatski infertilitet", "geni asocirani sa trombofilijom", "VDR gen", "multilokusne interakcije" ]
[ "idiopathic infertility", "genes associated with thrombophilia", "VDR gene", "multilocus interactions" ]
[ "Analiza polimorfizama gena za receptor za vitamin D i gena asociranih sa trombofilijom kod žena sa idiopatskim infertilitetom" ]
[ "Analysis of the polymorphisms in vitamin D receptor gene and genes associated with thrombophilia in women with idiopathic infertility" ]
doctoralThesis
[ "BY-NC-ND" ]
Стојковић, Оливер; Зељић, Катарина; Стаменковић, Горана; Ђуровић, Јелена Ж.; Aнализа полиморфизама гена за рецептор за витамин Д и гена асоцираних са тромбофилијом код жена са идиопатским инфертилитетом; Aнализа полиморфизама гена за рецептор за витамин Д и гена асоцираних са тромбофилијом код жена са идиопатским инфертилитетом;
[ "https://nardus.mpn.gov.rs/bitstream/id/1240/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1241/IzvestajKomisije15286.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1240/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1241/IzvestajKomisije15286.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_8938" ]
[ "" ]
[ "" ]
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[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/9383
123456789-9383.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:17529/bdef:Content/download
no
Kvalitativna i kvantitativna analiza humanih bioloških tragova minimalnih graničnih količina u forenzičkim analizama DNK
Qualitative and quantitative assesment of biological traces in forensic DNA analysis
[ "Keckarević, Dušan" ]
[ "" ]
Ostojić, Lana
2018-04-23T09:30:08
[ "2018-04-23T09:30:08", "2020-07-03T08:06:10" ]
2017-01-22
[ "https://nardus.mpn.gov.rs/handle/123456789/9383", "http://eteze.bg.ac.rs/application/showtheses?thesesId=5723", "https://fedorabg.bg.ac.rs/fedora/get/o:17529/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025183666" ]
[ "Ćelije kože ostavljene na površini bilo kog predmeta nakon kontakta sa njim mogu biti izvor DNK materijala. Ova vrsta biološkog materijala, u odnosu na količinu DNK koju je moguće izolovati iz nje, obično je mnogo manje izdašna u odnosu na tragove koji se rutinski analiziraju (krv, semena tečnost, pljuvačka). Iako visoko osetljive tehnike analize omogućavaju dobijanje rezultata iz minimalnih količina DNK, još uvek postoje znatne poteškoće u radu sa ovakvim uzorcima, i to iz više razloga. Različit kvalitet i kvantitet izolovane DNK, te posebno izraženi stohastički efekti samo su neki od njih. Zbog toga je proces analize DNK materijala izolovan iz ovakvih uzoraka posebno kompleksan. Biološki uzorci ove vrste se vrlo često nalaze u izuzetno malim količinama (doslovno tragovima), što se postojećim rutinskim metodama analize DNK ne mogu pouzdano interpretirati. U cilju poboljšavanja rezultata analize DNK materijala dobijenog izolovanjem biološkog materijala zaostalog na površini dodirnutih predmeta, analizirano je više od 700 otisaka prstiju. Svaki korak procesa je testiran posebno u cilju dobijanja STR profila koji su bar 70% kompletni, te stoga mogu biti uneti u baze podataka. Kompletnost profila je određivana na osnovu broja dobijenih alela iz 15 testiranih lokusa (najviše moguće 30 alela). Testirani su sledeći koraci u proceduri dobijanja DNK profila: prikupljanje uzoraka, izolovanje DNK, umnožavanje STR lokusa kao i detekcija proizvoda umnožavanja kapilarnom elektroforezom. Ćelije su prikupljene korišćenjem mikrosfera, lepljivih traka, lepila ili pamučnih briseva natopljenih različitim rastvorima. DNK materijal je izolovan korišćenjem interno razvijenih metoda i/ili komercijalno dostupnih kompleta hemikalija za njenu izolaciju. Izolovana DNK je umnožavana Identifiler® kompletom, korišćenjem različitog broja PCR ciklusa..." ]
[ "Skin flakes left on an object after it has been touched or handled could be a source of DNA. These skin flakes tend to be deposited in considerably smaller amounts than from routinely tested cells of blood, semen or saliva. Although, highly sensitive DNA analysis procedures are able to provide results from trace amounts of DNA there are still some fundamental difficulties inherent to these samples, including variability in quality and quantity of extracted DNA and exaggerated stochastic effects, making it hard to reliably interpret DNA profiles of these samples. These types of samples could also carry skin flakes in trace, which currently applied methodology of testing frequently cannot interpret. In order to improve the results from touched DNA samples, over 700 fingerprints were tested. Each step of the workflow for genotyping was assessed with the goal to generate STR profiles that were at least 70% complete and therefore database eligible. The profiles were calculated from the number of obtained alleles with a maximum of 30 for the 15 amplified STR loci. The steps evaluated in the workflow included sample collection, DNA extraction, STR amplification and detection utilizing capillary electrophoresis. Cells were collected using microglobes, tapes, glues, or cotton swabs moistened with different solutions. DNA extraction was assessed with methods designed in the laboratory and commercially available extraction kits. Extracted DNA was amplified with Identifiler® kits using various number of PCR cycles. These comparisons led to the best method that generated a database eligible STR profiles from almost 70% of tested fingerprints. This method suggested collection of fingerprints by swabbing with cotton swab moistened in detergent solution, then extracting DNA using a commercially available extraction kit that uses enzyme activated at a high temperature, followed by amplification at higher PCR cycle number and analysis at longer injection time and higher voltage during capillary electrophoresis..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]
Универзитет у Београду
[ "fingerprints", "touched DNA", "short tandem repeats (STRs)", "biological mixtures", "micromanipulations" ]
[ "" ]
[ "Kvalitativna i kvantitativna analiza humanih bioloških tragova minimalnih graničnih količina u forenzičkim analizama DNK" ]
[ "Qualitative and quantitative assesment of biological traces in forensic DNA analysis" ]
doctoralThesis
[ "BY-NC-SA" ]
Кецкаревић, Душан; Остојић, Лана; Квалитативна и квантитативна анализа хуманих биолошких трагова минималних граничних количина у форензичким анализама ДНК; Квалитативна и квантитативна анализа хуманих биолошких трагова минималних граничних количина у форензичким анализама ДНК;
[ "https://nardus.mpn.gov.rs/bitstream/id/1296/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1297/IzvestajKomisije16026.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1296/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1297/IzvestajKomisije16026.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_9383" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/2010
123456789-2010.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:9213/bdef:Content/download
no
Модел за оцену еколошке исправности кућа за индивидуално становање на подручју Београда
Model for environmental assessment of single family houses in Belgrade area
[ "Jovanović-Popović, Milica" ]
[ "Radivojević, Ana", "Pucar, Mila" ]
Kosanović, Saja
2016-01-05T11:43:37
[ "2016-01-05T11:43:37", "2020-07-02T16:24:30" ]
2012-12-27
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=1675", "https://nardus.mpn.gov.rs/handle/123456789/2010", "https://fedorabg.bg.ac.rs/fedora/get/o:9213/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=43987983" ]
[ "Основна сврха истраживања спроведеног у докторској дисертацији јесте дефинисање методологије јединственог процеса свеобухватног еколошког вредновања кућа за индивидуално становање на подручју Београда. У складу са тим, модел за оцену еколошке исправности формиран је прво као општи, а затим су из њега, због карактеристика сектора индивидуалног становања на подручју Београда, изведена три посебна модела, и то за оцену: новопројектованих, обновљених и постојећих кућа за индивидуално становање. Модел је структуиран по: категоријама, подкатегоријама и критеријумима (најпрецизнијим темама) оцене еколошке исправности. За одређивање категорија, које представљају основна поља оцене еколошке исправности, пресудан значај имали су еколошки утицаји кућа за индивидуално становање на животну средину, док су се карактеристике подручја Београда, постојећа домаћа и инострана регулатива и друге смернице, као и развијени инострани модели одразили на дефинисање садржаја тих категорија. Сви дефинисани критеријуми у моделу подељени су на обавезне услове и мере са добровољним испуњењем којима су додељени еколошки поени. Сабирањем броја додељених еколошких поена, прво за припадајуће критеријуме, затим подкатегорије и коначно категорије добија се укупни број еколошких поена додељен кући чија се еколошка исправност испитује. Успостављен је минимални праг еколошке исправности испод којег кућа која се оцењује не може бити окарактерисана као еколошки исправна, а изнад којег се, у зависности од броја додељених поена, она сврстава у један од три нивоа еколошке исправности. Кући која је постављена у најоптималнији положај према окружењу (са минималним токовима од природе и ка њој) биће додељен максимални број еколошких поена. На тај начин дефинисана је идеална комбинација примењених мера, релевантних за подручје Београда и за тип кућа за индивидуално становање. Након што су дефинисане структура модела и методологија оцене, креиран је електронски алат у рачунарском програму EXCEL, чиме је створеном методу дата употребна вредност. Рад алата тестиран је на примерима постојећих кућа за индивидуално становање на подручју Београда, чиме је утврђено да је модел функционалан, а да су резултати који се преко њега добијају тачни..." ]
[ "The aim of the research conducted in this doctoral dissertation was to define methodology for developing a system for comprehensive environmental assessment of individual houses in the area of Belgrade. According to this, model for environmental assessment was initially formed as general. Three separate models for assessments of new, renewed and existing houses, based on characteristics of individual housing sector in Belgrade, were derived later. Model structure is consisted of categories, subcategories and criteria (the most precise items) of environmental assessment. Environmental impacts of individual houses were found as the most relevant factor for determination of categories (basic fields of environmental assessment). Characteristics of the area of Belgrade, existing domestic and international legislative and other sources, as well as internationally developed models, on the other side, all influenced the content of categories. All defined criteria were divided on prerequisites and voluntary measures which carry a certain number of ecological points. By summing the numbers of ecological points, first by criteria, then subcategories and finally categories, the total number of achieved ecological points is obtained. Minimum threshold below which the house cannot be defined as environmentally correct is set; above this threshold, according to the total number of the obtained ecological points, the house will be classified among three set levels of environmental quality. Maximum number of ecological points will be given to the house set in optimal position towards environment (with minimal flows towards nature and from it). Therefore, the ideal combination of applied measures relevant for the area of Belgrade and for the type of individual houses is determined. Defined model structure and methodology for assessment opened the road to the creation of electronic tool in EXCEL computer program by which the practical value has been given to the model. The tool was tested on examples of existing individual houses in Belgrade; it is confirmed that the model is functional and that the obtained results are correct. The structure of general model, from which three separate were later derived, was created in segments; from one side, this enabled simplified separation of variants, and from other, more important, integration of life cycle with criteria of environmental assessment..." ]
application/pdf
sr
Универзитет у Београду, Архитектонски факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "еколошки поен", "категорија оцене еколошке исправности", "критеријум за оцену еколошке исправности", "кућа за индивидуално становање" ]
[ "ecological point", "category of environmental assessment", "criteria for the environmental assessment", "individual house" ]
[ "Модел за оцену еколошке исправности кућа за индивидуално становање на подручју Београда", "Model for environmental assessment of single family houses in Belgrade area" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Јовановић-Поповић, Милица; Радивојевић, Aна; Пуцар, Мила; Косановић, Саја; Model za ocenu ekološke ispravnosti kuća za individualno stanovanje na području Beograda;
[ "https://nardus.mpn.gov.rs/bitstream/id/806/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/806/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2010" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/9460
123456789-9460.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:17626/bdef:Content/download
no
Uloga HMGB1 proteina u modifikaciji i integraciji signalnih puteva apoptoze i autofagije u ćelijama jetre pacova sa indukovanim dijabetesom
The role of HMGB1 protein in the modification and integration of signaling pathways of apoptosis and autophagy in liver cells of diabetic rats.
[ "Grigorov, Ilijana" ]
[ "Matić, Gordana", "Poznanović, Goran", "Grigorov, Ilijana", "Matić, Gordana" ]
Petrović, Anja M.
2018-05-24T09:51:45
[ "2018-05-24T09:51:45", "2020-07-03T08:06:11" ]
2017-09-27
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=5769", "https://nardus.mpn.gov.rs/handle/123456789/9460", "https://fedorabg.bg.ac.rs/fedora/get/o:17626/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025176754" ]
[ "Ćelijska smrt je jedan od uzroka patoloških promena u jetri tokom dijabetesa. Protein koji može imati važnu ulogu u ovim procesima, bilo kao marker nekroze ili aktivator signalnih puteva koji vode ćelijskoj smrti ili preživljavanju, je HMGB1. U vanćelijsku sredinu HMGB1 dospeva iz nekrotičnih, oštećenih ćelija i aktiviranih imunskih ćelija. U ovoj doktorskoj disertaciji ispitivana je uloga ovog proteina u određivanju profila ćelijske smrti u jetri pacova sa dijabetesom kroz njegov doprinos u modulaciji procesa apoptoze i autofagije. Rezultati ovog istraživanja pokazali su da je nivo HMGB1 proteina u serumu i jetri pacova sa dijabetesom u korelaciji sa nivoom oštećenja jetre i nekroze, što ga svrstava među potencijalne biološke markere za praćenje dijabetičnih komplikacija u jetri. Na aktivaciju procesa apoptoze i autofagije HMGB1 protein deluje dvojako, kroz aktivaciju nizvodnih signalnih puteva pokrenutih interakcijama vanćelijskog HMGB1 sa TLR4 i RAGE receptorima, i kroz uticaj citoplazmatskog HMGB1 na formiranje autofagozoma kroz interakcije sa Beklinom1. Pokazano je da nivo vanćelijskog HMGB1 proteina i njegov doprinos profilu ćelijske smrti u jetri zavisi od intenziteta prisutnog oksidativnog stresa. U uslovima oksidativnog stresa prisutnog u nelečenom dijabetesu, HMGB1 favorizuje puteve apoptoze i doprinosi ćelijskoj smrti i oštećenju jetre. Smanjenjem oksidativnog stresa i nivoa vanćelijskog HMGB1 melatoninom ili etil piruvatom, favorizuje se delovanje citoplazmatskog HMGB1 u pravcu protektivne autofagije i uklanjanja oštećenih unutarćelijskih struktura. Ovi podaci ukazuju na terapeutski značaj melatonina i etil piruvata u regulaciji aktivnosti HMGB1 u dijabetesu i na ulogu HMGB1 proteina u regulaciji i povezivanju apoptoze i autofagije." ]
[ "Cell death underlies hepatic pathological changes in diabetes. HMGB1 is a protein with important roles in these changes, whether as a necrotic marker or a pathway activator determining cell death or survival. HMGB1 is released into the extracellular space by damaged or necrotic cells and activated immune cells. The role of HMGB1 in determining the profile of cell death in the liver of diabetic rats was studied by examining its role in the modulation of apoptosis and autophagy. The levels of HMGB1 protein in the serum and liver of diabetic rats correlate with the degree of necrosis and liver damage, thus rendering HMGB1 as a potential biological marker of diabetic complications. To activate apoptosis and autophagy, HMGB1 protein acts by stimulating downstream signaling pathways triggered by interactions of extracellular HMGB1 with TLR4 and RAGE receptors, and through the activity of cytoplasmic HMGB1 on the formation of autophagosomes via interaction with Beclin1, which has a central role in autophagy. Extracellular expression of HMGB1 and its contribution to the cell death profile in the liver depends on the level of oxidative stress. In oxidative stress accompanying untreated diabetes, HMGB1 promotes apoptosis and resulting liver damage. The reduction of oxidative stress by melatonin and HMGB1 release by ethyl pyruvate stimulates the cytoprotective role of cytosolic HMGB1 in autophagy activation These results points to the therapeutic significance of melatonin and ethyl pyruvate in the regulation of HMGB1 activity in diabetes, and the role of HMGB1 in the regulation and interplay between apoptosis and autophagy." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173020/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]
Универзитет у Београду
[ "HMGB1 protein", "dijabetes", "jetra", "oksidativni stres", "apoptoza", "autofagija", "nekroza" ]
[ "HMGB1 protein", "diabetes", "liver", "oxidative stress", "apoptosis", "autophagy", "necrosis" ]
[ "Uloga HMGB1 proteina u modifikaciji i integraciji signalnih puteva apoptoze i autofagije u ćelijama jetre pacova sa indukovanim dijabetesom" ]
[ "The role of HMGB1 protein in the modification and integration of signaling pathways of apoptosis and autophagy in liver cells of diabetic rats." ]
doctoralThesis
[ "BY-NC-SA" ]
Григоров, Илијана; Матић, Гордана; Познановић, Горан; Григоров, Илијана; Матић, Гордана; Петровић, Aња М.; Улога ХМГБ1 протеина у модификацији и интеграцији сигналних путева апоптозе и аутофагије у ћелијама јетре пацова са индукованим дијабетесом; Улога ХМГБ1 протеина у модификацији и интеграцији сигналних путева апоптозе и аутофагије у ћелијама јетре пацова са индукованим дијабетесом;
[ "http://nardus.mpn.gov.rs/bitstream/id/1304/IzvestajKomisije16867.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1303/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1304/IzvestajKomisije16867.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1303/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_9460" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/9461
123456789-9461.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:17628/bdef:Content/download
no
Efekat mutacije gena VHL na nivo ekspresije proteina mTOR signalnog puta u svetloćelijskom karcinomu bubrega
The effect of VHL gene mutation on the expression levels of mTOR signaling pathway proteins in clear cell renal cell carcinoma
[ "Damjanović, Svetozar" ]
[ "Matić, Gordana", "Brajušković, Goran", "Đorđević, Ana", "Marković-Lipkovski, Jasmina" ]
Ilić, Bojana B.
2018-05-24T09:51:45
[ "2018-05-24T09:51:45", "2020-07-03T08:06:17" ]
2018-03-30
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=5771", "https://nardus.mpn.gov.rs/handle/123456789/9461", "https://fedorabg.bg.ac.rs/fedora/get/o:17628/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025184946" ]
[ "Sporadični svetloćelijski karcinomi bubrega asocirani su sa mutacijama tumor supresorskog gena VHL kod 60-80 % pacijenata. U odsustvu funkcionalnog proteina VHL dolazi do akumulacije faktora indukovanih hipoksijom koji regulišu ekspresiju gena uključenih u procese angiogeneze, eritropoeze, ćelijskog metabolizma i preživljavanja. Pored specifičnog genetičkog profila i povišenog nivoa HIF-2α svetloćelijske karcinome bubrega karakteriše glikolitički metabolički put i povišena aktivnost mTOR signalnog puta. Nepovoljan energetski status i hipoksija blokiraju aktivnost mTOR signalnog puta preko proteina AMPK i REDD1. Ova studija imala je za cilj da utvrdi mogću asocijaciju mutacionog statusa gena VHL sa patohistološkim parametrima, nivoom HIF-2α iRNK i nivoom proteina HIF-2α, mTOR, TSC1, TSC2, eIF4E-BP1, p70S6K1, AMPK, REDD1 i PDK3 u svetloćelijskom karcinomu bubrega. Studija je obuhvatala 47 pacijenata kojima je urađena totalna nefrektomija zbog prisustva svetloćelijskog karcinoma bubrega. Genomska DNK izolovana iz uzoraka venske krvi i somatska DNK izolovana iz uzoraka tumorskog i neizmenjenog tkiva bubrega upotrebljene su za određivanje mutacionog statusa gena VHL i gubitka heterozigotnosti 3p lokusa sekvenciranjem, metodom amplifikacije višestruko ligiranih proba i fragmentnom analizom mikrosatelitnih markera. Metilacioni status promotora gena VHL ispitan je metil specifičnom PCR reakcijom nakon bisulfitne modifikacije DNK. Kvantitativni RT-PCR primenjen je za relativnu kvantifikaciju HIF-2α iRNK u tumorskom i neizmenjenog tkivu bubrega. Kvantitafikacija ekspresije proteina HIF-2α, mTOR, TSC1, eIF4E-BP1, p70S6K1, AMPK, REDD1 i PDK3 utvrđena je Western blot metodom. Semikvantitativna imunohistohemijska analiza korišćena je za utvrđivanje nivoa ekpresije proteina TSC2..." ]
[ "Sporadic clear cell renal carcinoma is associated with mutations of the VHL gene in 60-80 % patients. The absence of a functional VHL protein leads to accumulation of hypoxia-inducible factors which regulate expression of genes involved in angiogenesis, erythropoiesis, cell metabolism and survival. Specific genetic background, overexpression of HIF-2α transcription factor as well as glycolytic metabolism and upregulated activity of mTOR pathway are the main characteristics of clear cell renal carcinoma. The lack of energy and hypoxia inhibit mTOR signaling through activity of AMPK and REDD1 proteins. The aim of present study was to determine association of VHL gene mutational status with pathohistological parameters, expression level of mRNA HIF-2α and expression levels of mTOR, TSC1, TSC2, eIF4E-BP1, p70S6K1, AMPK, REDD1 and PDK3 proteins in clear cell renal carcinoma. The study included 47 patients who underwent radical nephrectomy due to unilateral kidney tumor. DNA was extracted from peripheral venous blood, tumorous and corresponding healthy renal tissue. The VHL gene mutational status and loss of heterozigosity of 3p loci were detected using DNA sequencing, MLPA and analysis of microsatellite markers. The VHL gene promoter methylation analysis was examined by MS-PCR after bisulphite modification of DNA. Quantitative real time PCR was performed for relative quantification of HIF-2 alpha mRNA. Expression levels of HIF- 2α, mTOR, TSC1, eIF4E-BP1, p70S6K1, AMPK, REDD1 and PDK3 proteins was determined using Western blot method. Immunohistochemistry was performed for semiquantitative analysis of TSC2 protein. Alteration of VHL gene and/or changes of 3p loci were detected in 37/47 (78.7 %) analysed tumorous samples..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "svetloćelijski karcinom bubrega", "gen VHL", "mTOR signalni put", "ćelijski metabolizam" ]
[ "clear cell renal cell carcinoma", "VHL gene", "mTOR signaling pathway", "cellular metabolism" ]
[ "Efekat mutacije gena VHL na nivo ekspresije proteina mTOR signalnog puta u svetloćelijskom karcinomu bubrega" ]
[ "The effect of VHL gene mutation on the expression levels of mTOR signaling pathway proteins in clear cell renal cell carcinoma" ]
doctoralThesis
[ "BY-NC-ND" ]
Дамјановић, Светозар; Матић, Гордана; Марковић-Липковски, Јасмина; Ђорђевић, Aна; Брајушковић, Горан; Илић, Бојана Б.; Ефекат мутације гена ВХЛ на ниво експресије протеина мТОР сигналног пута у светлоћелијском карциному бубрега; Ефекат мутације гена ВХЛ на ниво експресије протеина мТОР сигналног пута у светлоћелијском карциному бубрега;
[ "https://nardus.mpn.gov.rs/bitstream/id/1317/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1318/IzvestajKomisije16869.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1317/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1318/IzvestajKomisije16869.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_9461" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/2003
123456789-2003.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:8131/bdef:Content/download
no
Модел класификације елемената архитектонских објеката у техничкој документацији - формирање и примена
A model for classifying building elements in the design documentation - establishment and application
[ "Ćosić, Gordana" ]
[ "Đokić, Lidija", "Svetel, Igor" ]
Jurenić, Tatjana S.
2016-01-05T11:43:17
[ "2016-01-05T11:43:17", "2020-07-02T16:24:20" ]
2013-06-06
[ "https://nardus.mpn.gov.rs/handle/123456789/2003", "http://eteze.bg.ac.rs/application/showtheses?thesesId=1216", "https://fedorabg.bg.ac.rs/fedora/get/o:8131/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=512978833" ]
[ "објеката у техничкој документацији. Природа процеса пројектовања и изградње објеката захтева стварање и сакупљање информација широког спектра у свакој фази животног циклуса архитектонског објекта. Специфичности овог процеса захтевају јасне формате у којима ће информације конзистентно да се преносе из фазе у фазу. Правци развоја грађења и тенденције високоразвијених система у свету указују да је потребно обратити више пажње процесима који претходе самој градњи, у које сигурно спада и подробно планирање и усклађивање форми израде техничке документације, што води најважнијим циљевима у изградњи, а то су унапређење квалитета, скраћење времена и контролисани трошкови грађења. Ниво обрађености техничке документације, која је неопходни елемент процеса уговарања за изградњу објекта, потребно је да одговара како домаћим, тако и светским стандардима и да омогући лакше управљање процесом изградње и бржу и прецизнију процену трошкова грађења у раним фазама инвестиционог циклуса. Анализом стања у домаћој и светској пракси искристалисао се и обликовао предмет истраживања: недостатак јасно формулисане, научно засноване номенклатуре и класификације, која би омогућила стандардизацију у изради техничке документације, а самим тим и унапредила односе и процесе у свим фазама животног циклуса архитектонских објеката.Форматизована и стандардизована техничка документација постаје заједничка нит за пренос података, описа, праћење и евалуацију кроз све фазе пројекта. Реализовано истраживање има за резултат дефинисање конкретних критеријума, чијом применом је формиран стандардизован модел класификације, базиран на елементима зграде према месту и функцији на објекту, неопходан је у циљу омогућавања јасне комуникације између учесника у изградњи у погледу обима послова и трошкова у свакој фази животног циклуса зграде. Овако формиран модел класификације елемената у техничкој документацији представља константу у изузетно динамичном процесу пројектовања и грађења архитектонског објекта. Истовремено, модел нуди флексибилност у даљем развоју технологије и примене материјала, с обзиром да, постављајући архитектонски објекат у логичну, централну позицију у процесима инвестиционе изградње, није зависан од економских и технолошких промена које су неминовне..." ]
[ "The research is devoted to the creation of a model for classifying building elements and related sitework in the design documentation. The nature of the design and construction process requires creation and collection of a wide range of information at each stage of the of the building life cycle. Specifics of this process requires a clear format which will provide transmission of the information consistently among different phases. Directions of construction development and tendencies of the world’s highly developed systems indicate that it is necessary to pay more attention to the process preceding the construction, which certainly includes the detailed planning and coordination of design and building documentation, leading to the most important goals, such as improving quality, reducing time and controling construction costs. The processing of the design documentation is a necessary element in the contracting process for building construction and it is required in order to comply with both national and international standards and provide an easier management of the building process, as well as faster and more accurate construction cost estimates in the early stages of the investment cycle. Analysis of the state of local and global practice crystallized and formed the subject of research: the lack of clearly formulated, science-based nomenclature and classification, which would allow standardization in the development of the design documentation, and therefore improve the relations and processes in all stages of the building life cycle. Structured and standardized design documentation becomes the common link for the transmision of data, descriptions, monitoring and evaluation informations throughout all phases of the project. The research has resulted in the definition of specific criteria according to which a standardized model of classification was formed, based on the elements of the building according to the place and function of the building. The purpose of the model is to provide clear communication between participants in the building process in terms of workload and costs in each stage of the building life cycle. A model of classification based on building elements becomes a design documentation constant base in the highly dynamic process of designing and constructing. At the same time, the model provides flexibility in the further development of building technology and the application of the materials. The model is not dependent on the economic and technological changes that are inevitable, since, it sets the building into a logical, central position in the construction process..." ]
application/pdf
sr
Универзитет у Београду, Архитектонски факултет
[ "info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/36038/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]
Универзитет у Београду
[ "модел", "класификација елемената", "техничка документација", "архитектонски објека" ]
[ "model", "classification of building elements", "design documentation" ]
[ "Модел класификације елемената архитектонских објеката у техничкој документацији - формирање и примена", "A model for classifying building elements in the design documentation - establishment and application" ]
[ "" ]
doctoralThesis
[ "BY-NC-SA" ]
Ћосић, Гордана; Ђокић, Лидија; Светел, Игор; Јуренић, Татјана С.; Model klasifikacije elemenata arhitektonskih objekata u tehničkoj dokumentaciji - formiranje i primena;
[ "https://nardus.mpn.gov.rs/bitstream/id/779/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/779/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2003" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/9039
123456789-9039.pdf
no
no
https://fedorabg.bg.ac.rs/fedora/get/o:16858/bdef:Content/download
no
Molecular characterization and phylogenetic relationships among European Aphidius Nees (Hymenoptera, Braconidae, Aphidiinae)
Молекуларна карактеризација и филогенетски односи европских врста рода Aphidius Nees (Hymenoptera, Braconidae, Aphidiinae)
[ "Tomanović, Željko" ]
[ "Mitrović, Milana", "Petrović, Anđeljko" ]
Jamhour, Aiman M.
2017-12-20T13:08:34
[ "2017-12-20T13:08:34", "2020-07-03T08:06:22" ]
2017-10-30
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=5468", "https://nardus.mpn.gov.rs/handle/123456789/9039", "https://fedorabg.bg.ac.rs/fedora/get/o:16858/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025181362" ]
[ "У род Aphidius класификоване су многе врсте које су економски значајне као биолошки агенти за контролу штетних врста биљних вашију у стакленицима и на отвореном пољу. Обзиром да биологија, екологија и таксономски статус многих врста нису довољно истражени, класификација врста унутар рода се континуирано ревидира. Делимичне секвенце гена митохондријске ДНК цитохром оксидазе субјединица I (mtCOI) и једарног гена за фактор елонгације 1-α a (EF1-α) су маркери коришћени у истраживању таксономског статуса и филогенетиских односа 33 врсте рода Aphidius у асоцијацији са раличитим врстама ваши и биљака домаћина сакупљених са ширег арела у Европи. Филогенетске анализе су потврдиле да је род Aphidius парафилетички. На филогенетском стаблу конструисаном maximum likelihood методом јасно је издвајање следећа 22 таксона као засебних врста: A. erysimi, A. sonchi, A. linosiphonis, A. hieraciorum, A. arvensis, A. balcanicus, A. phalangomyzi, A. banksae, A. uzbekistanicus, A. sussi, A. silvaticus, A. avenae, A. rosae, A. ericaphidis, A. eadyi, A. viaticus, A. schimitscheki, A. ribis, A. setiger, A. asteris, A. matricariae и A. urticae Поред ових „добрих“ врста, издовјило се још пет клада: i) A. salicis и A. aquilus; ii) A. funebris, A. tanacetarius, A. absinthii; iii) A. ervi и A. microlophii; iv) A. chaetosiphonis и A. hortensis; v) A. rubi и A. rhopalosiphi. Врсте у овим кладама се не могу јасно идентификовати на основу митохондријског и једарног маркера. Неуспех у идентификацији врста применом ових маркера може се приписати адаптивној дивергенцији услед ширења круга домаћина или станишта и специјацији, или митохондријалној интрогресији приликом хибридизације примерака сродних врста. За поједине врсте сугерише се ревизија таксономског статуса, примењујући савремени интегративни приступ. Молекуларна идентификација mtCOI открила је у оквиру комплекса врста A. urticae три криптичне врсте A. urticae s. str., A. rubi и A. silvaticus. Такође, ДНК баркодинг метод је потврдио први пут у Европи присуство врсте A. ericaphidis." ]
[ "The genus Aphidius includes many species of economic importance that are used as biocontrol agents against numerous pest aphids in greenhouses and under open field conditions. However, classification within this genus is constantly revisited, in view of the fact that the biology, ecology and taxonomic status of many species are still understudied. Partial sequences of the cytochrome oxidase subunit I mitochondrial gene (mtCOI) and Elongation factor 1-α nuclear gene (EF1-α) were used to explore the taxonomic status and phylogenetic relationships of 33 European species from the genus Aphidius in different aphid /plant host associations over a wide area of distribution. Phylogenetic analyses clarified that Aphidius is in fact a paraphyletic group. Topology of the maximum likelihood tree showed separation of 22 taxa as independent species: A. erysimi, A. sonchi, A. linosiphonis, A. hieraciorum, A. arvensis, A. balcanicus, A. phalangomyzi, A. banksae, A. uzbekistanicus, A. sussi, A. silvaticus, A. avenae, A. rosae, A. ericaphidis, A. eadyi, A. viaticus, A. schimitscheki, A. ribis, A. setiger, A. asteris, A. matricariae and A. urticae. Besides “good” species, five more clades were distinguished: i) A. salicis and A. aquilus; ii) A. funebris, A. tanacetarius, A. absinthii; iii) A. ervi and A. microlophii; iv) A. chaetosiphonis and A. hortensis; v) A. rubi and A. rhopalosiphi. Taxa within five clades could not be clearly discriminated as separate species based on either mtCOI or EF1-α. Failure of the two markers to delimit these taxa could be attributed either to adaptive divergence due to host and/or habitat range expansion and speciation or to mitochondrial introgression via hybridization of sibling species. In any event, it is suggested that their taxonomic status be re-visited using an integrative approach. Molecular characterization revealed cryptic taxa associated with different hosts within the A. urticae group. Re-descriptions of A. urticae s. str., A. rubi and A. silvaticus are given. Also, mtDNA barcoding identified the presence of A. ericaphidis for the first time in Europe" ]
application/pdf
en
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Aphidius", "mtDNA barcoding", "speciation", "elongation factor 1-α", "paraphyletic group" ]
[ "Aphidius", "ДНК баркодинг", "специјација", "фактор елонгације 1-α", "парафилетичка група" ]
[ "Molecular characterization and phylogenetic relationships among European Aphidius Nees (Hymenoptera, Braconidae, Aphidiinae)" ]
[ "Молекуларна карактеризација и филогенетски односи европских врста рода Aphidius Nees (Hymenoptera, Braconidae, Aphidiinae)" ]
doctoralThesis
[ "BY-NC-ND" ]
Томановић, Жељко; Митровић, Милана; Петровић, Aнђељко; Јамхоур, Aиман М.;
[ "http://nardus.mpn.gov.rs/bitstream/id/1343/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1344/IzvestajKomisije15367.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1344/IzvestajKomisije15367.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1343/Disertacija.pdf", "https://fedorabg.bg.ac.rs/fedora/get/o:16858/bdef:Content/download" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_9039" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/5086
123456789-5086.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:10896/bdef:Content/download
no
Промена урбанистичко-архитектонске парадигме на примеру београдских стамбених насеља
Change of urban and architectural paradigm exemplified by Belgrade housing estates: the period of Yugoslav late socialism (1965-1991)
[ "Đokić, Vladan" ]
[ "Lazović, Zoran", "Bojanić, Petar" ]
Kušić, Aleksandar V.
2016-04-10T11:21:53
[ "2016-04-10T11:21:53", "2020-07-02T16:23:58" ]
2014-10-13
[ "https://nardus.mpn.gov.rs/handle/123456789/5086", "http://eteze.bg.ac.rs/application/showtheses?thesesId=2752", "https://fedorabg.bg.ac.rs/fedora/get/o:10896/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=513012625" ]
[ "Овај рад истражује динамику и правце промене урбанистичко-архитектонске парадигме на примеру стамбених насеља пројектованих и/или изграђених у Београду између 1965. и 1991. године. Временски оквир рада обухвата три крупна историјска процеса који су обликовали теорију и праксу социјалистичког самоуправљања у Југославији: привредну реформу из 1965. године, доношење Устава СФРЈ 1974. године и демонтирањe социјалистичког друштвено-симболичког поретка током касних 80-их година. Промена парадигме се у раду разматра у контексту ових процеса, то јест, у погледу маргиналнe позиције коју је партиципација крајњих корисника заузимала у оквиру промене парадигме. Уколико је самоуправљање теоријски постављено као врхунски облик партиципације и уколико је инострани развој парадигми присутних у београдском урбанизму и архитектури периода обухватио концепте попут отворене форме, партиципативног пројектовања и самоградње, онда је циљ овог рада утврђивање позиције коју је промена парадигме заузимала у ширем оквиру социјалистичке производње простора, а у светлу недостатка партиципације крајњих корисника. Ослањајући се на тезу француског неомарксисте Анри Лефевра (Henri Lefebvre) о производњи простора као друштвеној пракси, те на савремене теорије непотпуности друштва, рад полази од претпоставке да је самоуправљање, без обзира на маргиналност партиципације, било део урбанизма и архитектуре београдских стамбених насеља, то јест, да су се касномодернa, постмодернa и традиционалнa парадигма периода перманентно односиле према самоуправљању и антагонизмима који су одређивали његову теорију и праксу. У погледу искључивања крајњих корисника из урбанистичког и архитектонског пројектовања рад полази од претпоставке да је овај поступак грађен упоредним деловањем више актера, укључујући оне у чије име су малобројни примери отворене форме, партиципативног пројектовања и самоградње и наступали..." ]
[ "This dissertation investigates dynamics and directions of the change of urban and architectural paradigm exemplified by the housing estates designed and/or built in Belgrade between 1965 and 1991. The dissertation’s time frame envelops three major historical processes shaping the theory and practice of socialist self-management in Yugoslavia: the economic reform of 1965, the enactment of the 1974 SFRY Constitution and the deconstruction of socialist socio-symbolic order during the late 1980s. The paradigm change is discussed in the context of the processes, that is, in the light of the marginal position occupied within it by participation of end users. If self-management was conceived theoretically as the ultimate form of participation and if the foreign development of paradigms present in Belgrade urbanism and architecture of the period involved concepts such as open form, participatory design and self-built, than the goal of this dissertation is to establish the position paradigm change occupied within the wider framework of socialist production of space, in the light of a lack of user participation. Supported by the thesis of the French neo-Marxist philosopher Henri Lefebvre, regarding the production of space as a social practice, and by contemporary theories of society’s lack of closure, the dissertation’s hypothesis is that self-management, regardless of the marginality of participation, was indeed part of the urbanism and architecture of Belgrade housing estates, or that late modern, postmodern, and traditional paradigms of the period had constantly addressed self-management and the antagonisms which had determined its theory and practice. Regarding the exclusion of end users from urban and architectural design, the dissertation’s hypothesis is that this procedure was shaped by the parallel strivings of several actors, including those in whose name rare examples of open form, participatory design and self-built operated..." ]
application/pdf
sr
Универзитет у Београду, Архитектонски факултет
[ "info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/36035/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "стамбена насеља", "касномодерна парадигма", "постмодерна парадигма", "традиционална парадигма", "партиципација", "самоуправљање", "југословенски касни социјализам", "антагонизам", "друштвено-симболички поредак", "Београд" ]
[ "housing estates", "late modern paradigm", "postmodern paradigm", "traditional paradigm", "participation", "self-management", "Yugoslav late socialism", "antagonism", "socio-symbolic order", "Belgrade" ]
[ "Промена урбанистичко-архитектонске парадигме на примеру београдских стамбених насеља", "Change of urban and architectural paradigm exemplified by Belgrade housing estates: the period of Yugoslav late socialism (1965-1991)" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Ђокић, Владан; Лазовић, Зоран; Бојанић, Петар; Кушић, Aлександар В.; Promena urbanističko-arhitektonske paradigme na primeru beogradskih stambenih naselja;
[ "http://nardus.mpn.gov.rs/bitstream/id/704/Disertacija759.pdf", "http://nardus.mpn.gov.rs/bitstream/id/705/Aleksandar_Kusic_Ocena_Referat_AF.pdf", "https://nardus.mpn.gov.rs/bitstream/id/704/Disertacija759.pdf", "https://nardus.mpn.gov.rs/bitstream/id/705/Aleksandar_Kusic_Ocena_Referat_AF.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_5086" ]
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https://nardus.mpn.gov.rs/handle/123456789/10116
123456789-10116.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:18584/bdef:Content/download
no
Funkcionalnost i obrazac signalnih puteva Lajdigovih ćelija odraslih pacova nakon primene anaboličkih androgenih steroida
Functionality and pattern of signaling pathways of Leydig cells in adult rats after administration of anabolic androgenic steroids
[ "Andrić, Silvana" ]
[ "Jasnić, Nebojša", "Kostić, Tatjana", "Đorđević, Jelena", "Andrić, Silvana" ]
Srbovan, Maja M.
2018-11-09T16:41:29
[ "2018-11-09T16:41:29", "2020-07-03T08:06:31" ]
2018-09-27
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6124", "https://nardus.mpn.gov.rs/handle/123456789/10116", "https://fedorabg.bg.ac.rs/fedora/get/o:18584/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025205682" ]
[ "Lajdigove ćelije intersticijuma testisa su primarno mesto sinteze androgenih hormona, a dominantno testosterona (T). Ovaj hormon je zajedno sa svojim metabolitom dehidrotestosteronom (DHT) neophodan, kako za produženje vrste obezbeđivanjem pravilnog razvoja i funkcionisanja muškog reproduktivnog sistema, tako i za opšte zdravlje individue. U cilju tretiranja brojnih kliničkih poremećaja, kao i u svrhu kontracepcije, sintestisani su derivati androgena čija se primena u klinici zasniva na dejstvu koje ostvaruju posredstvom androgenih i/ili anaboličkih efekata, te su zajedničkim imenom nazvani anabolički androgeni steroidi (AAS). Nažalost, AAS se često zloupotrebljavaju, ne samo od strane profesionalnih i rekreativnih sportista, nego i velike populacije adolescenata, iako je dobro poznato da njihova upotreba u neterapeutske svrhe može izazvati niz neželjenih zdravstvenih posledica. Stoga su AAS svrstani u grupu farmakoloških preparata čija je upotreba strogo regulisana i nisu dostupni bez lekarskog recepta. Uprkos široko rasprostranjenoj kliničkoj upotrebi i zloupotrebi AAS, kao i velikom interesovanju naučne zajednice za ovu problematiku, nisu u potpunosti razjašnjeni molekulski događaji koji su posledica njihove kratkoročne i dugoročne primene. S obzirom na značaj T za reprodukciju i produženje vrste, ali i zdravlje i kvalitet života indivudue, kao i široku primenu i zloupotrebu T i njegovih derivata, neophodno je okarakterisati precizne molekulske događaje nastale kao posledica poremećene homeostaze T. Ovo je važno zbog toga što, prema trenutno dostupnoj literaturi, ne postoji dovoljan broj podataka o funkcionalnosti i obrascima signalnih puteva Lajdigovih ćelija, čija je osnovna uloga sinteza i sekrecija T. Stoga je glavni cilj ovog istraživanja bio da ispita funkcionalnost i obrasce signalnih puteva važnih za održavanje steroidogene funkcije Lajdigovih ćelija, narušene primenom egzogenih agonista i/ili antagonista T u in vivo ili in vitro uslovima. U tu svrhu primenjen je derivat T, testosteron-enantat (TE), koji se najčešće upotrebljava u kliničkoj praksi, ali se i u najvećoj meri zloupotrebljava i najprodavaniji je na tzv. “crnom” tj. ilegalnom tržištu. Ovakav model daje mehanistički pristup, ali ima i translacioni aspekt, s obzirom na to da su upotrebljavane doze/koncentracije T koje se koriste u kliničkoj praksi ili se zloupotrebljavaju. Rezultati su pokazali da in vivo aplikacija TE inhibira steroidogenu funkciju Lajdigovih ćelija odraslih pacova, kao i relativnu ekspresiju gena za komponente cAMP-PRKA signalizacije, kao glavnog regulatora steroidogeneze ovih ćelija..." ]
[ "Leydig cells of testis interstitium represent the major site for synthesis of androgenic hormone, primarily testosterone (T). This hormone, together with its metabolite dihydrotestosterone (DHT), is required, not only for the continuation of the species by ensuring proper development and functioning of male reproductive system, but for overall health of an individual as well. For the purpose of treatment of multiple clinical disorders, as well as for contraception purposes, androgen derivatives have been synthesized, the clinical application of which is based on the influence they have through anabolic and/or androgenic effects, thus having a common name anabolic androgenic steroids (AASs). Unfortunately, AASs are often abused, not only by professional and recreational athletes, but by a large population of adolescents as well, although it is well known that non-therapeutic use thereof may cause a series of adverse health effects. Therefore, AASs are classified into a group of pharmacological preparations, the use of which is strictly regulated and which are not available without a medical prescription. Despite widespread clinical use and abuse of AASs, as well as the great interest shown by the scientific community in this field, molecular events resulting from their short-term and long-term use have not been fully clarified. Given the importance of T for reproduction and continuation of the species, but also for health and quality of life of an individual, and the widespread use and abuse of T and its derivatives, it is necessary to characterize precise molecular events resulting from disturbed T homeostasis. This is important because, according to the currently available literature, there is insufficient data on the functionality and patterns of signaling pathways of Leydig cells, the basic role of which is the synthesis and secretion of T. Therefore, the main aim of this study is to examine the functionality and patterns of signaling pathways relevant for maintaining the steroidogenic function of Leydig cells, impaired by the use of exogenous T agonists and/or antagonists under in vivo or in vitro conditions. To this end, a T derivative known as testosterone-enanthate (TE) is applied, which is most commonly used in clinical practice, but is largely abused and best-selling product in the so-called \"black\" or illegal market. Such model provides a mechanistic approach, but has also a translational aspect, since the applied T doses/concentrations are used in clinical practice or are abused. The results have shown that in vivo application of TE inhibits the steroidogenic function of Leydig cells in adult rats, as well as gene expression for cAMP-PRKA signaling components, being the main regulator of steroidogenesis of such cells..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173057/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]
Универзитет у Београду
[ "Lajdigove ćelije", "steroidogeneza", "testosteron", "androgeni receptor", "membranski potencijal mitohodrija", "HSD3B", "ARR19", "ADCY-cAMP-PRKA signalizacija", "PRL-JAK-STAT signalizacija", "androgeni anabolički steroidi" ]
[ "Leydig cells", "steroidogenesis", "testosterone", "androgen receptor", "mitochondrial membrane potential", "HSD3B", "ARR19", "ADCY-cAMP-PRKA signaling", "PRL-JAK-STAT signaling", "anabolic androgenic steroids" ]
[ "Funkcionalnost i obrazac signalnih puteva Lajdigovih ćelija odraslih pacova nakon primene anaboličkih androgenih steroida" ]
[ "Functionality and pattern of signaling pathways of Leydig cells in adult rats after administration of anabolic androgenic steroids" ]
doctoralThesis
[ "BY-NC-SA" ]
Aндрић, Силвана; Aндрић, Силвана; Јаснић, Небојша; Костић, Татјана; Ђорђевић, Јелена; Србован, Маја М.; Функционалност и образац сигналних путева Лајдигових ћелија одраслих пацова након примене анаболичких андрогених стероида; Функционалност и образац сигналних путева Лајдигових ћелија одраслих пацова након примене анаболичких андрогених стероида;
[ "https://nardus.mpn.gov.rs/bitstream/id/1380/IzvestajKomisije18290.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1379/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1379/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1380/IzvestajKomisije18290.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10116" ]
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https://nardus.mpn.gov.rs/handle/123456789/12041
123456789-12041.pdf
yes
no
https://nardus.mpn.gov.rs/bitstream/id/36132/Disertacija.pdf
no
Prinos i kvalitet biomase lucerke (Medicago sativa L.) u zavisnosti od sistema gajenja
Yield and quality of alfalfa biomass (Medicago sativa L.) as affected by production management
[ "Ćupina, Branko" ]
[ "Erić, Pero", "Milić, Dragan" ]
Katanski, Snežana
2020-02-26T16:23:22
[ "2020-02-26T16:23:22", "2020-07-03T13:33:20" ]
2017-11-14
[ "https://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija150408781386796.pdf?controlNumber=(BISIS)104977&fileName=150408781386796.pdf&id=10448&source=NaRDuS&language=sr", "https://nardus.mpn.gov.rs/handle/123456789/12041", "https://www.cris.uns.ac.rs/record.jsf?recordId=104977&source=NaRDuS&language=sr", "https://www.cris.uns.ac.rs/DownloadFileServlet/IzvestajKomisije150408782009797.pdf?controlNumber=(BISIS)104977&fileName=150408782009797.pdf&id=10449&source=NaRDuS&language=sr" ]
[ "Značaj lucerke u srpskoj poljoprivredi, kao i njena velika tržišna vrednost nameće stalnu potrebu za unapređenjem proizvodnje ove krmne biljke koja zauzima velike površine, kako u svetu tako i u našoj zemlji. Sveobuhvatni cilj doktorske disertacije je da se oceni uticaj različitih sistema gajenja (režima kosidbe) na prinos i hranljivu vrednost lucerke, doprinese rešavanju praktičnih pitanja u proizvodnji lucerke (redefiniše optimalna količina semena za setvu) i odredi fenološka faza u momentu kosidbe u kojoj se postiže balans prinosa i kvaliteta suve materije lucerke. Trogodišnje istraživanje (2010-2012) je izvedeno na oglednim poljima Odeljenja za krmno bilje Instituta za ratarstvo i povrtarstvo u Novom Sadu, na dva lokaliteta (Rimski Šančevi i Čenej). Poljski ogled je postavljen po slučajnom blok sistemu u tri ponavljanja na oba lokaliteta. U radu su analizirane četiri sorte lucerke (Banat VS, Nijagara, NS Medijana ZMS V i NS Alfa); dve setvene norme od 8 i 16 kg/ha i tri sistema kosidbe u zavisnosti od fenološke faze razvoja biljaka: I – rani (10% cvetalih biljaka), II – srednje rani (50% cvetalih biljaka), i kasni (početak zametanja mahuna). Tretmani su aranžirani po sistemu podeljenih parcela (split-plot metod), a kao osnovna parcela (veličina 72 m2) korišćena je setvena norma, dok su sve ostale kombinacije sorti i režima kosidbe tretirane kao podparcele (veličina 6 m2). Dimenzije podparcele su 5 m dužina i 1,2 m širina, sa međurednim razmakom od 20 cm. Razmak između blokova je 1 m. Među ispitivanim sortama nije bilo značajne razlike u visini prinosa i komponentama prinosa suve materije lucerke, kao ni u pogledu njihovog kvaliteta. Različite količine semena za setvu lucerke, od 8 i 16 kg/ha, nisu uticale na prinos suve materije kao ni na komponente prinosa (visina biljaka, udeo lista u prinosu, broj izdanaka i broj biljaka). U umereno-kontinentalnim klimatskim uslovima, kakvi vladaju u većem delu u Republike Srbije, nema opravdanja povećanja setvene norme iznad 16 kg/ha jer se setvom većih količina semena ne obezbeđuje dugoročna korist u proizvodnji lucerke. Sistemi gajenja, odnosno kosidba lucerke u različitim fazama starosti useva (početak cvetanja, puno cvetanje i faza zelenih mahuna) imala je značajan efekat na prinos, komponente prinosa i hranljivu vrednost ispitivanih sorti lucerke. Najveći prinos zelene krme ostvaren je pri ranoj kosidbi u fazi 10% cvetalih biljaka, dok je prinos suve materije bio identičan pri košenju u fazi 10% i 50% cvetalih biljaka i iznosio je 15,9 t ha-1. Sistem kosidbe značajno je uslovljavao visinu biljaka, pri čemu su najviše biljke (81,5 cm) izmerene pri košenju u fazi zelenih mahuna. Najveći udeo lista od 500,7 g/kg suve materije dobijen je kada se lucerka kosila na početku cvetanja. Učestalost kosidbe je značajno uticala na broj izdanaka po jedinici površine, koji se značajno smanjio pri kosidbi u kratkim vremenskim intervalima, odnosno u fazi početka cvetanja biljaka. Fenološka faza razvoja u momentu kosidbe nije uticala na broj biljaka po jedinici površine. Gustina lucerišta bila je slična pri košenju u fazi 10% i 50% cvetalih biljaka (240,7 i 246,3 bilj./m2). Rezultati analize o hranljivoj vrednosti lucerke ukazuju na značajan uticaj uslova sredine i faze razvoja u momentu kosidbe za sadržaj sirovih proteina, dok je sadržaj vlakana bio uslovljen samo fazom u momentu košenja (sistemom kosidbe). Bolji kvalitet suve materije lucerke ostvaren je u vlažnoj godini (2010) u poređenju sa sušnom godinom (2011). Sadržaj sirovih proteina u suvoj materiji lucerke se smanjivao zbog smanjenja udela lista, dok se sadržaj NDF-a, ADF-a i ADL-a povećavao od faze početka cvetanja do faze zelenih mahuna. Kosidbom lucerke u četvorootkosnom i petootkosnom sistemu dobijeni su slični prinosi suve materije (15,9 t ha-1), dok se primenom trootkosnog sistema kosidbe ne iskorišćava genetički potencijal NS sorti, dobija se loš kvalitet i svarljivost (veći udeo vlakana), i dolazi do značajnog smanjenja prinosa suve materije lucerke (13,1 t ha-1). Kosidba lucerke u početku cvetanja biljaka (pet otkosa u godini) daje bolji kvalitet suve materije, odnosno veći sadržaj proteina i manji sadržaj vlakana (NDF, ADF i ADL)." ]
[ "The importance of alfalfa in Serbian agriculture, as well as its high market value, imposes a constant need for the improvement of this forage crop production that occupies large areas both in the world and in our country. The overall objective was to evaluate the effect of different growing systems (cutting regimes) on yield and nutritional value of alfalfa, contribute to solving practical issues in alfalfa production (redefine the optimal amount of seed for sowing) and specify the phenological stage in the moment of cutting when the balance of yield and dry matter quality of alfalfa is achieved. Three-year study (2009-2012) was carried out on experimental fields of Forage Crops Department of the Institute of Field and Vegetable Crops in Novi Sad, at two sites (Rimski Šančevi and Čenej). Field trial was set up in a randomized block design with three replications at both sites. Four cultivars of alfalfa were studied in this dissertation (Banat VS, Nijagara, NS Medijana ZMS V and NS Alfa); two sowing rates from 8 and 16 kg/ha and three cutting systems depending on crop phenological stage of development: I – early (10% of flowering plants), II – medium early (50% of flowering plants), and late (beginning of pod setting). Treatments were arranged by split-plot method, and the basic plot (size 72 m2) was used as the sowing rate, while all the other combinations of cultivars and cutting regimes were treated as sub-plots (6 m2). Sub-plot dimensions were 5 m in length and 1.2 m in width, with interrow distance of 20 cm. Inter-block distance was 1 m. There were no significant differences in yield and yield components of alfalfa dry matter, or in their quality, among the tested cultivars. Different amounts of seed for alfalfa sowing, from 8 and 16 kg/ha, did not affect the dry matter yield, or the yield components (crop height, leaf ratio in yield, number of shoots, and number of plants). In moderate-continental climatic conditions, which are present in the majority of the Republic of Serbia, there is no justification for increasing sowing rate above 16 kg/ha, because higher sowing rates do not provide long-term benefit in alfalfa production. Growing systems or cutting alfalfa in different stages of crop maturity (beginning of flowering, full flowering and green pods stage) had significant effect on the yield, yield components and nutritional value of tested alfalfa cultivars. The highest yield of green forage was achieved in early cutting at the stage of 10% flowering plants, while the dry matter yield was identical in cutting at the stage of 10% and 50% flowering plants and amounted to 15.9 t ha-1. Cutting system significantly conditioned plant height, when the highest plants (81.5 cm) were measured in cutting at the stage of green pods. The highest leaf ratio from 500.7 g/kg of dry matter was obtained when alfalfa was cut at the beginning of flowering. Frequency of cutting significantly affected the number of shoots per area unit, which notably decreased during cutting in short time intervals, i.e. at the beginning of flowering stage. Phenological development stage at the moment of cutting did not affect the number of plants per area unit. Alfalfa field density was similar to cutting at the stage of 10% and 50% flowering plants (240.7 and 246.3 plants/m2). Results of alfalfa nutritional value analysis indicated the significant effect of environmental conditions and development stage at the moment of cutting on crude protein content, while fibre content was conditioned only by the stage at the moment of cutting (cutting system). Alfalfa dry matter had better quality in wet year (2010) than in dry year (2011). Content of crude proteins in alfalfa dry matter decreased due to the reduction of leaf ratio, while the contents of NDF, ADF and ADL increased from the beginning of flowering stage to the stage of green pods. Similar yields of dry matter were achieved by cutting alfalfa with four and five cuts (15.9 t ha-1), while three cuts system did not use the genetic potential of NS cultivars, lead to bad quality and digestibility (higher fibre ratio) and to significant yield decrease of alfalfa dry matter (13.1 t ha-1). Cutting alfalfa at the beginning of flowering (five cuts a year) gives better quality of dry matter, i.e. higher protein content and lower fibre content (NDF, ADF i ADL)." ]
sr (latin script)
Универзитет у Новом Саду, Пољопривредни факултет
[ "info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31024/RS//", "info:eu-repo/grantAgreement/MESTD/MPN2006-2010/20090/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]
Универзитет у Новом Саду
[ "Lucerka", "sistem gajenja", "setvena norma", "prinos", "kvalitet" ]
[ "alfalfa", "cutting management", "seeding rate", "yield", "quality" ]
[ "Prinos i kvalitet biomase lucerke (Medicago sativa L.) u zavisnosti od sistema gajenja" ]
[ "Yield and quality of alfalfa biomass (Medicago sativa L.) as affected by production management" ]
doctoralThesis
[ "BY-NC-SA" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/36132/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/36133/IzvestajKomisije.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_12041" ]
[ "" ]
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[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/10117
123456789-10117.pdf
yes
yes
https://fedorabg.bg.ac.rs/fedora/get/o:18585/bdef:Content/download
no
Uloga mikrobiote i limfnog tkiva creva u otpornosti pacova soja Albino Oksford na indukciju eksperimentalnog autoimunskog encefalomijelitisa
Role of gut microbiota and gut-associated lymphoid tissue in resistance of Albino Oxford rats to experimental autoimmune encephalomyelitis induction
[ "Miljković, Đorđe" ]
[ "Božić Nedeljković, Biljana", "Golić, Nataša", "Miljković, Đorđe" ]
Stanisavljević, Suzana M.
2018-11-09T16:41:29
[ "2018-11-09T16:41:29", "2020-07-03T08:06:32" ]
2018-07-10
[ "https://nardus.mpn.gov.rs/handle/123456789/10117", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6125", "https://fedorabg.bg.ac.rs/fedora/get/o:18585/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025190578" ]
[ "Multipla skleroza je hronična, inflamacijska, neurodegenerativna, demijelinizirajuća bolest centralnog nervnog sistema (CNS). Etiologija multiple skleroze još uvek nije poznata, ali je sve više podataka koji pokazuju ulogu crevne mikrobiote u patogenezi bolesti. Više podataka o značaju crevne mikrobiote u autoimunosti CNS dobijeno je istraživanjima na eksperimentalnom autoimunskom encefalomijelitisu (EAE), životinjskom modelu multiple skleroze. U EAE-u se autoimunski odgovor protiv CNS-a pokreće u limfnim čvorovima koji dreniraju mesto imunizacije, gde se CD4+ T ćelije specifične za antigene CNS-a aktiviraju i diferenciraju u Th (engl. T helper) 1 ćelije koje produkuju IFN-γ i Th17 ćelije koje produkuju IL-17. Kada dospeju u CNS ove Th ćelije bivaju reaktivirane od strane lokalnih antigen-prezentujućih ćelija. Nakon reaktivacije, encefalitogene Th ćelije pokreću i održavaju inflamaciju unutar CNS-a. Albino oksford (AO) pacovi su veoma otporni na indukciju EAE-a. S druge strane, Dark Agouti (DA) pacovi razvijaju EAE čak i nakon blage imunizacije. U dosadašnjim istraživanjima je pokazano da se AO pacovi u odnosu na DA pacove razlikuju po slaboj aktivaciji Th1 i Th17 ćelija u relevantnim limfnim čvorovima u odgovoru na encefalitogenu imunizaciju. Takođe, pokazano je da relativno mali broj encefalitogenih ćelija infiltrira CNS AO pacova. Do sada nije vršeno ispitivanje uloge GALT-a i mikrobiote creva u otpornosti pacova soja AO na indukciju EAE-a. Sve je više istraživanja koja pokazuju da mikroorganizmi creva imaju važnu ulogu u regulaciji imunskog odgovora kroz interakciju sa limfnim tkivom creva (GALT, engl. gut-associated lymphoid tissue). U istraživanjima na EAE-u je pokazano da određeni mikroorganizmi creva pospešuju tok bolesti, dok drugi ostvaruju terapeutske efekte modulacijom odnosa Th1 i Th17 encefalitogenih ćelija i regulatornih T ćelija (Treg). Poznato je i da encefalitogene CD4+ T ćelije migriraju u GALT, uključujući mezenterične limfne čvorove i Pejerove ploče gde se mogu diferencirati u Treg..." ]
[ "Multiple sclerosis is a chronic inflammatory neurodegenerative demyelinating disease of the central nervous system (CNS). Etiology of multiple sclerosis is still unknown, but data are showing that gut microbiota plays an important role in the pathogenesis of this disease. Information about the significance of gut microbiota in CNS autoimmunity has been dominantly obtained in studies in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. The autoimmune response against CNS starts in lymph nodes draining the site of immunization in EAE. There, CD4+ T cells are being activated and differentiated into Th1 (helper T cells) and Th17 cells that produce IFN-γ and IL-17, respectively. When Th cells arrive in the CNS, they are reactivated by local antigen-presenting cells. After the reactivation, these encephalitogenic T cells initiate and propagate CNS inflammation. Albino Oxford (AO) rats are highly resistant to EAE induction. On the other hand, Dark Agouti (DA) rats develop EAE even after the mild immunization. In our earlier studies it was shown that AO rats had less activated Th1 and Th17 cells in the relevant lymph nodes in response to encephalitogenic immunization, unlike DA rats. Also, it was shown that relatively small number of encephalitogenic cells infiltrated CNS in AO rats. Up until now, the studies of role of GALT and gut microbiota in resistance of AO rats in EAE induction have not been conducted. It has been increasingly appreciated that gut microbiota plays an important role in the regulation of the immune response through interaction with the cells of gut-associated lymphoid tissue (GALT). In EAE studies specific gut microorganisms have been proposed to promote the disease, while others have been shown to have therapeutic effects by modulating ratio and activity of encefalitogenic Th1 and Th17 cells and regulatory T cells (Treg). Encefalitogenic cells can also migrate to GALT, including mesenteric lymph nodes and Peyer’s patches, where they can be re-differentiated into Treg..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173013/RS//", "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173035/RS//" ]
[ "openAccess" ]
[ "" ]
Универзитет у Београду
[ "eksperimentalni autoimunski encefalomijelitis", "Albino Oksford pacovi", "mikrobiota creva", "limfno tkivo creva" ]
[ "experimental autoimmune encephalomyelitis", "Albino Oxford rat", "gut microbiota", "gut-associated lymphoid tissue" ]
[ "Uloga mikrobiote i limfnog tkiva creva u otpornosti pacova soja Albino Oksford na indukciju eksperimentalnog autoimunskog encefalomijelitisa" ]
[ "Role of gut microbiota and gut-associated lymphoid tissue in resistance of Albino Oxford rats to experimental autoimmune encephalomyelitis induction" ]
doctoralThesis
[ "ARR" ]
Миљковић, Ђорђе; Миљковић, Ђорђе; Божић Недељковић, Биљана; Голић, Наташа; Станисављевић, Сузана М.; Улога микробиоте и лимфног ткива црева у отпорности пацова соја Aлбино Оксфорд на индукцију експерименталног аутоимунског енцефаломијелитиса; Улога микробиоте и лимфног ткива црева у отпорности пацова соја Aлбино Оксфорд на индукцију експерименталног аутоимунског енцефаломијелитиса;
[ "https://nardus.mpn.gov.rs/bitstream/id/1386/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1387/IzvestajKomisije18291.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1386/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1387/IzvestajKomisije18291.pdf", "https://fedorabg.bg.ac.rs/fedora/get/o:18585/bdef:Content/download" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10117" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/10118
123456789-10118.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:18588/bdef:Content/download
no
Карактеризација структуре и функције HMGB1 протеина у јетри пацова током експериментално изазваног дијабетеса типа 1
Structural and functional characterization of HMGB1 protein in rat liver during experimentally-induced diabetes type 1
[ "Martinović, Vesna" ]
[ "Matić, Gordana", "Grigorov, Ilijana", "Martinović, Vesna", "Matić, Gordana" ]
Jovanović Stojanov, Sofija M.
2018-11-09T16:41:30
[ "2018-11-09T16:41:30", "2020-07-03T08:06:33" ]
2018-07-10
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6128", "https://nardus.mpn.gov.rs/handle/123456789/10118", "https://fedorabg.bg.ac.rs/fedora/get/o:18588/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025190322" ]
[ "Оксидативни стрес и хронична инфламација сматрају се главним узроцима појаве дијабетичних компликација, међу којима су и оштећења јетре. Важну улогу медијатора ових процеса може имати ендогени протеин HMGB1, који у ванћелијску средину доспева из некротичних, оштећених и активираних ћелија. Како је у дијабетесу понашање HMGB1 протеина слабо изучавано, у овој докторској дисертацији испитиван је допринос HMGB1 оштећењима јетре пацова са стрептозотоцином-изазваним ДТ1. Показано је да ниво оштећења јетре током дијабетесa корелише са присуством ванћелијског HMGB1. Овај протеин, у дијабетичној јетри, бива структурно модификован ацетилацијом, фосфорилацијом и O-GlcNAc гликозилацијом што корелише са његовим изласком из једра ћелија у цитоплазму и повећањем његовог присуства у јетри и серуму. Резултати у вези са снижавањeм нивоа ванћелијског HMGB1 третманом дијабетичних пацова мелатонином или етил пируватом, указују да HMGB1 доприноси оштећењу јетре у дијабетесу одржавањем стања хроничне инфламације, стишавањем антиоксидативне одбране и стишавањем регенерације. Ванћелијски HMGB1 кроз интеракције са TLR4 рецептором активира MAPК/NF-κB p65 и ЈАК1/STAT3 сигналне путеве, доприносећи повећању продукције проинфламацијских цитокина TNF-α и IL-6 и акутно-фазног протеина хаптоглобина. Подстицањем NF-κB p65 инфламацијског пута, HMGB1 делује негативно на цитопротективни одговор у дијабетичној јетри тако што онемогућава активност Nrf2 протеина, одговорног за стишавање инфламације и продукцију антиоксидативних ензима. На стишавање регенеративног потенцијала јетре, активирана HMGB1/TLR4 оса утиче преко увећања присуства негативних регулатора ћелијског циклуса - протеина p53 и p21, и смањењем нивоа циклина D1. Добијени резултати указују на сложеност деловања HMGB1 протеина у дијабетесу и на значај спречавања ослобађања HMGB1 или блокаде HMGB1/TLR4 осе у циљу одлагања настанка оштећења јетре." ]
[ "Oxidative stress and chronic inflammation are considered to be the main causes of diabetic complications, one of which is liver damage. An important mediator of these processes may be the endogenous HMGB1 protein, when released into the extracellular environment from the necrotic, damaged or activated cells. As the HMGB1 role in diabetes was insufficiently studied, in this doctoral dissertation the contribution of HMGB1 to liver damage of streptozotocin-induced diabetic rats was investigated. It has been shown that the level of liver damage in diabetes correlates with the presence of extracellular HMGB1. In diabetic liver, this protein is structurally modified by acetylation, phosphorylation, and O-GlcNAc glycosylation, which correlates with its translocation from the nucleus to the cytoplasm and an increase in its presence in the liver and serum. Reduction of the level of extracellular HMGB1 by melatonin or ethyl pyruvate treatment of diabetic rats, shows that HMGB1 contributes to diabetic liver damage by maintaining a chronic inflammation, by lowering antioxidant defense and by reducing regeneration. Extracellular HMGB1 activates MAPK/NF-κB p65 and JAK1/STAT3 signaling pathways through interactions with the TLR4 receptor, thus contributing increased production of proinflammatory cytokines TNF-α and IL-6 and the acute-phase protein, haptoglobin. By stimulating the NF-κB p65 inflammatory pathway, HMGB1 acts negatively on the cytoprotective response of the diabetic liver, by disabling Nrf2 protein activity, which is responsible for reduction of inflammation and antioxidant enzymes production. Activated HMGB1/TLR4 axis reduces regenerative potential of the liver by increasing the presence of negative cell cycle regulators - proteins p53 and p21, and also by decreasing the level of cyclin D1. The obtained results indicate the complexity of HMGB1 protein action in diabetes and underlines the importance of preventing the release of HMGB1 or blockage of HMGB1/TLR4 axis in order to delay the occurrence of liver damage." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173020/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]
Универзитет у Београду
[ "HMGB1 протеин", "дијабетес", "јетра", "оксидативни стрес", "структурне модификације", "инфламација", "регенерација" ]
[ "HMGB1 protein", "diabetes", "liver", "oxidative stress", "structural modifications", "inflammation", "regeneration" ]
[ "Карактеризација структуре и функције HMGB1 протеина у јетри пацова током експериментално изазваног дијабетеса типа 1" ]
[ "Structural and functional characterization of HMGB1 protein in rat liver during experimentally-induced diabetes type 1" ]
doctoralThesis
[ "BY-NC-SA" ]
Мартиновић, Весна; Мартиновић, Весна; Матић, Гордана; Матић, Гордана; Григоров, Илијана; Јовановић Стојанов, Софија М.; Karakterizacija strukture i funkcije HMGB1 proteina u jetri pacova tokom eksperimentalno izazvanog dijabetesa tipa 1;
[ "http://nardus.mpn.gov.rs/bitstream/id/1393/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1394/IzvestajKomisije18294.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1394/IzvestajKomisije18294.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1393/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10118" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/10119
123456789-10119.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:18601/bdef:Content/download
no
Efekti hronične primene losartana i tempola u eksperimentalnom modelu fokalno segmentne glomeruloskleroze kod spontano hipertenzivnih pacova
Effects of chronic losartan and tempol treatments on experimental focal segmental glomerulosclerosis in spontaneously hypertensive rats
[ "Grujić Milanović, Jelica" ]
[ "Đorđević, Jelena", "Mihailović-Stanojević, Nevena" ]
Karanović, Danijela J.
2018-11-09T16:41:31
[ "2018-11-09T16:41:31", "2020-07-03T08:06:34" ]
2018-09-28
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6135", "https://nardus.mpn.gov.rs/handle/123456789/10119", "https://fedorabg.bg.ac.rs/fedora/get/o:18601/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025205426" ]
[ "Dosadašnja istraživanja pokazala su da renin angiotenzin sistem (RAS) ima važnu ulogu u patogenezi i progresiji fokalno segmentne glomeruloskleroze (FSGS). Oksidativni stres je prisutan u hroničnoj bubrežnoj slabosti i doprinosi progresiji bolesti. Povezanost oksidativnog stresa i RAS u procesima progresije FSGS još je nedovoljno razjašnjena. Stoga su istraživanja u okviru ove disertacije bila usmerena ka ispitivanju efekata hronične primene tempola (sakupljač slobodnih radikala, SOD mimetik), losartana (blokator receptora za angiotenzin II tipa 1, AT1R), kao i njihove kombinacije, na usporavanje progresije FSGS izazvane adrijamicinom (ADR) kod spontano hipertenzivnih (SH) pacova. Životinje su inicijalno deljene u dve eksperimentalne grupe, kontrolnu (SHC) i grupu koja je primila ADR 2 mg/kg i.v. dva puta u intervalu od 21 dan. Nakon druge injekcije ADR, životinje su dobijale vodu (SHADR), losartan (SHADR+L), tempol (SHADR+T) i kombinovani tretman (SHADR+T+L) gavažom. Na kraju šeste nedelje tretmana vršena su hemodinamska merenja i uzorkovanje krvi, urina i bubrega. Na osnovu biohemijskih parametara vršena je procena lipidnog statusa i bubrežne funkcije. Urađena je histopatološka analiza bubrega. Imunihistohemijskom metodom ispitivani su proteini citosketeta, nestin i vimentin, čije izmenjene ekspresije su pokazatelj oštećenja bubrežnog tkiva. Matriksna metaloproteinaza-1 (MMP-1), koja učestvuje u degradaciji komponenti vanćelijskog mariksa i očuvanju integriteta glomerula, određivana je ELISA metodom. Ispitivani su parametri oksidativnog stresa: lipidna peroksidacija i nivo karbonilovanih proteina (PCOs); aktivnosti enzima antioksidativnog sistema: superoksid dismutaze (SOD), katalaze (CAT), glutation peroksidaze (GPx) i glutation reduktaze (GR); kao i antioksidativni kapacitet. Primenom imunohistohemijske, Western blot i ELISA metode u bubrezima su određivane ekspresije Nox2 i Nox4 izoforme katalitičke subjedinice NADPH oksidaze, glavni izvor reaktivnih kiseoničnih vrsta u ćeliji, uključene u proces inflamacije. Ekspresija proteina tri izoforme azot-monoksid sintaze, inducibilna (iNOS), endotelna (eNOS) i neuronska (nNOS), određivane su Western blot i imunohistohemijskom metodom u tkivu bubrega. Određivani su ukupni metaboliti NO-a (nitriti, nitrati) u urinu i bubrezima..." ]
[ "Previous studies have shown that renin angiotensin system (RAS) plays an important role in the pathogenesis and progression of focal segmental glomerulosclerosis (FSGS). Oxidative stress is involved in the development and progression of chronic kidney disease. However, the association of oxidative stress and RAS in the progression of FSGS has not been completely elucidated. In this study we investigated the effects of chronic tempol (free radical scavenger, SOD mimetic), losartan (selective angiotensin II type 1 receptor (AT1R) blocker), and their combined treatment in slowing down the progression of FSGS, in spontaneously hypertensive (SH) rats with adriamycin (ADR) nephropathy. Animals were initially divided into two experimental groups: control (SHC) and group that received ADR 2 mg/kg i.v. twice in an interval of 21 days. After the second injection of ADR, the animals were given tap water (SHADR), losartan (SHADR+L), tempol (SHADR+T) or combined treatment (SHADR+T+L) by gavage. Hemodynamic measurements were performed, blood, urine, and kidney samples were taken for biochemical and histopathological analysis. Immunohistochemical method was used for protein expression and localization of the nestin and vimentin, proteins of cytoskeleton network, and change in their protein expression is a marker of cell injury. Matrix metalloproteinase-1 (MMP-1), which participates in the degradation of the extracellular matrix components and preserving the integrity of the glomerulus, was determined by the ELISA method. The parameters of oxidative stress: lipid peroxidation, protein carbonyl content (PCOs); antioxidant defense: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and antioxidant capacity were analysed. Immunohistochemical, Western blot and ELISA method were used for analysis of the Nox2 and Nox4 protein expression, isoforms of the catalytic subunit of NADPH oxidase, the main source of reactive oxygen species in the cell, involved in the inflammation process. Protein expression of three isoforms of azote monoxide synthase, inducible (iNOS), endothelial (eNOS), and neuronal (nNOS) were determined by Western blot and immunohistochemical method in the kidney. Total metabolites of NO (nitrites, nitrates) were measured in the kidney and urine..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175096/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]
Универзитет у Београду
[ "fokalno segmentna glomeruloskleroza", "adrijamicinska nefropatija", "spontano hipertenzivni pacovi", "blokator receptora za angiotenzin II tipa 1", "tempol", "oksidativni stres", "azot-monoksid sintaza" ]
[ "focal segmental glomerulosclerosis", "adriamycin nephropathy", "spontaneously hypertensive rats", "angiotensin II type 1 receptor", "tempol", "oxidative stress", "nitric oxide synthase" ]
[ "Efekti hronične primene losartana i tempola u eksperimentalnom modelu fokalno segmentne glomeruloskleroze kod spontano hipertenzivnih pacova" ]
[ "Effects of chronic losartan and tempol treatments on experimental focal segmental glomerulosclerosis in spontaneously hypertensive rats" ]
doctoralThesis
[ "BY-NC-SA" ]
Грујић Милановић, Јелица; Ђорђевић, Јелена; Михаиловић-Станојевић, Невена; Карановић, Данијела Ј.; Ефекти хроничне примене лосартана и темпола у експерименталном моделу фокално сегментне гломерулосклерозе код спонтано хипертензивних пацова; Ефекти хроничне примене лосартана и темпола у експерименталном моделу фокално сегментне гломерулосклерозе код спонтано хипертензивних пацова;
[ "https://nardus.mpn.gov.rs/bitstream/id/1400/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1401/IzvestajKomisije18301.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1400/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1401/IzvestajKomisije18301.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10119" ]
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https://nardus.mpn.gov.rs/handle/123456789/5462
123456789-5462.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:11154/bdef:Content/download
no
Експресионизам у архитектури XX века у Србији
Expressionism in XX century architecture in Serbia.
[ "Ignjatović, Aleksandar" ]
[ "Mako, Vladimir", "Kadijević, Aleksandar" ]
Alfirević, Đorđe I.
2016-06-11T12:16:36
[ "2016-06-11T12:16:36", "2020-07-02T16:24:55" ]
2015-07-01
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=2928", "https://nardus.mpn.gov.rs/handle/123456789/5462", "https://fedorabg.bg.ac.rs/fedora/get/o:11154/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=513031313" ]
[ "Када се у науци помиње реч „експресионизам”, у већини случајева се мисли на стилски правац, који се јавио у архитектури и уметностима почетком XX века и који је трајао до 1930. године. Са друге стране, постоји и шире тумачење појма, по коме експресионизам није само стилски правац, већ једна од кључних стваралачких тенденција, тј. темељни чинилац у свим уметностима током историје. Ослањајући се на шире тумачење, у раду је разматрана могућност постојања експресионизма у архитектонској култури у Србији током XX века и поред увреженог мишљења, да он није имао значајнијег утицаја на архитектуру у нашој средини. Критичком анализом теоријских ставова у литератури, размотрене су сличности и разлике између основних естетских категорија „експресије” и „експресивности”. Такође, је констатована њихова релација са феноменом експресионизма, а све са циљем јаснијег тумачења и евидентирања његове појаве у архитектури у Србији. Студијом најзначајнијих експресионистичких остварења у архитектури у свету, су екстраховани експресионистички принципи и обележја, чије је постојање истражено на референтним архитектонским примерима из Србије. Компаративном анализом одабраних архитектонских остварења, који су настали током XX века у Србији и који су у литератури означени као експресионистички, у истраживању је изведен закључак, да експресионизам представља темељни принцип и једну од кључних стваралачких тенденција у архитектури XX века у Србији. Такође, експресионизам се не јавља у истом периоду кад и у другим деловима Европе (1905-1930), већ је преломни тренутак за његову појаву почетак шездесетих година XX века, када се јављају први пројекти и реализације, код којих је експресионистички израз у целости спроведен, што је супротно ономе што је до сада важило у науци." ]
[ "When the word „expressionism” is mentioned in science, it usually refers to style movement which appeared in architecture and the arts at the beginning of the XX century and lasted until 1930. However, there is a wider interpretation of the term, which defines expressionism not only as a style movement but as one of the key creative tendencies, i.e. the essential factor in all arts throughout history. By relying on the wider interpretation, this paper discussed potential presence of expressionism in architectural culture in Serbia during the XX century, even though the prevailing opinion claimed that expressionism did not have a more significant influence on the architecture in Serbia. By critically analyzing theoretical positions in literature, we examined the similarities and differences between basic aesthetic categories: „expression” and „expressiveness”. We also confirmed their relation to the phenomenon of expressionism, with an aim of providing a clearer interpretation of it, as well as establishing its presence in Serbian architecture. By studying most prominent expressionist achievements in world architecture, we extracted expressionistic principles and characteristics whose presence we examined on leading architectural examples in Serbia. By comparative analysis of selected architectural objects, made in XX century in Serbia and marked in reference books as expressionist, our paper concludes that expressionism represents the key principle and one of the essential creative tendencies in XX century architecture in Serbia. Moreover, expressionism in Serbia did not emerge at the same time as in other parts of Europe (1905-1930), the turning point leading to its appearance was the beginning of the sixties in the XX century, when the first projects and realizations appeared, implementing complete expressionist articulation, which opposed the existing viewpoints in science." ]
application/pdf
sr
Универзитет у Београду, Архитектонски факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "архитектура", "експресионизам", "експресија", "експресивност" ]
[ "architecture", "expressionism", "expression", "expressivity" ]
[ "Експресионизам у архитектури XX века у Србији", "Expressionism in XX century architecture in Serbia." ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Игњатовић, Aлександар; Мако, Владимир; Кадијевић, Aлександар; Aлфиревић, Ђорђе И.; Ekspresionizam u arhitekturi XX veka u Srbiji;
[ "https://nardus.mpn.gov.rs/bitstream/id/881/Disertacija2731.pdf", "https://nardus.mpn.gov.rs/bitstream/id/882/Djordje_Alfirevic_Referat_AF.pdf", "http://nardus.mpn.gov.rs/bitstream/id/881/Disertacija2731.pdf", "http://nardus.mpn.gov.rs/bitstream/id/882/Djordje_Alfirevic_Referat_AF.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_5462" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/7883
123456789-7883.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:15111/bdef:Content/download
no
Efekat elektromagnetnog polja (50Hz, 0,25mT) na komponente adaptivne vrednosti i morfometriju krila kod Drosophila subobscura Collin, 1936 (Insecta, Diptera)
Effect of electromagnetic field (50 Hz, 0.25 mT) on fitness components and wing morphometrics of Drosophila subobscura Collin, 1936 (Insecta, Diptera)
[ "Stamenković-Radak, Marina" ]
[ "Stamenković-Radak, Marina", "Savić-Veselinović, Marija", "Kenig, Bojan", "Kurbalija-Novičić, Zorana" ]
Patenković, Aleksandra D.
2017-04-17T14:58:58
[ "2017-04-17T14:58:58", "2020-07-03T08:05:28" ]
2016-06-03
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=4807", "https://nardus.mpn.gov.rs/handle/123456789/7883", "https://fedorabg.bg.ac.rs/fedora/get/o:15111/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025098162" ]
[ "Od svog nastanka život na Zemlji se odvijao u prisustvu prirodnih elektromagnetnih polja (EMP) koja su relativno jednostavna i potiču od malobrojnih emitera elektromagnetne energije. Živa bića su se tokom svoje evolucije prilagodila ovom konstantnom sredinskom faktoru, ali su danas pored prirodnih, u sve većoj meri izložena EMP poreklom od izvora koje je stvorio čovek. Intenzitet i frekvencija ovih polja su daleko veći u odnosu na polja prirodnog porekla, a tu spadaju najvećim delom EMP mrežne frekvencije (u okviru dela spektra ekstremno niske frekvencije (ENF) od 50 ili 60 Hz), polja koji potiču od uređaja masovnih (RTV predajnici, 100-500 MHz) i mobilnih komunikacija (800-900 MHz i 1800-2200 MHz). EMP ENF imaju veliki biofizički značaj jer su sveprisutna u životnoj sredini usled prenosa i upotrebe električne energije. Elektromagnetna polja antropogenog porekla predmet su izučavanja različitih bioloških disciplina, ali pre svega sa aspekta zdravlja ljudi. Izlaganje organizama ELM ENF, čak i tokom kraćeg vremenskog perioda, ima za posledicu narušavanje ravnoteže bioloških sistema, kao i poremećaje na različitim nivoima organizacije, ali još uvek ne postoji opšte prihvaćena teorija o mehanizmima dejstva EMP ENF u biološkim sistemima. Pored toga, sredinske promene, usled prisustva EMP ENF, mogu imati uticaj na ekološke i evolucione procese koji utiču na stepen i obim promena u genetičkoj strukturi i evoluciji populacije. Stoga je od suštinskog značaja dublje razumevanje mehanizama koji omogućavaju organizmima da se manje ili više uspešno prilagođavaju sredinskim izazovima, kao i mogućnost da se predvide i prate odgovori populacija na promene u životnoj sredini koja ih okružuje. Tokom poslednje tri decenije izvedene su brojne studije na Drosophila sa ciljem ispitivanja bioloških efekata EMP ENF. Eksperimentalno izlaganje individualnih genotipova Drosophila EMP ENF tokom različitih faza životnog ciklusa, rezultuje različitim odgovorima koji su često oprečni, jer efekat delovanja EMP ENF na rast i metabolizam organizama, bilo inhibitorni, bilo stimulativni, zavisi od kompleksnih veza između frekvencije, jačine polja, dužine izlaganja, i stanja i tipa ćelija ili organizama..." ]
[ "" ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173012/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "elektromagnetno polje (EMP) ekstremno niske frekvencije (ENF)", "Drosophila subobscura", "komponente adaptivne vrednosti", "geometrijska morfometrija krila", "fenotipska plastičnost" ]
[ "extremely low frequency (ELF) electromagnetic field (EMF)", "Drosophila subobscura", "fitness components", "geometric morphometrics", "phenotypic plasticity" ]
[ "Efekat elektromagnetnog polja (50Hz, 0,25mT) na komponente adaptivne vrednosti i morfometriju krila kod Drosophila subobscura Collin, 1936 (Insecta, Diptera)" ]
[ "Effect of electromagnetic field (50 Hz, 0.25 mT) on fitness components and wing morphometrics of Drosophila subobscura Collin, 1936 (Insecta, Diptera)" ]
doctoralThesis
[ "BY-NC-ND" ]
Стаменковић-Радак, Марина; Курбалија-Новичић, Зорана; Савић-Веселиновић, Марија; Стаменковић-Радак, Марина; Кениг, Бојан; Патенковић, Aлександра Д.; Ефекат електромагнетног поља (50Хз, 0,25мТ) на компоненте адаптивне вредности и морфометрију крила код Дросопхила субобсцура Цоллин, 1936 (Инсецта, Диптера); Ефекат електромагнетног поља (50Хз, 0,25мТ) на компоненте адаптивне вредности и морфометрију крила код Дросопхила субобсцура Цоллин, 1936 (Инсецта, Диптера);
[ "https://nardus.mpn.gov.rs/bitstream/id/1077/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1078/IzvestajKomisije8283.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1077/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1078/IzvestajKomisije8283.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_7883" ]
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https://nardus.mpn.gov.rs/handle/123456789/2171
123456789-2171.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:9710/bdef:Content/download
no
Interakcija inicijacionog proteina ORC sa replikatorom regiona lamin B2
Interaction of initiation protein Orc with lamin B2 replication region
[ "" ]
[ "" ]
Kušić, Jelena
2016-01-05T11:48:35
[ "2016-01-05T11:48:35", "2020-07-03T08:07:27" ]
2005-07-01
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=1967", "https://nardus.mpn.gov.rs/handle/123456789/2171", "https://fedorabg.bg.ac.rs/fedora/get/o:9710/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=31423759" ]
[ "Inicijacija replikacije DNK kod eukariota započinje vezivanjem proteinskog kompleksa ORC za diskretna mesta u genomu nazvana ori-sekvence ili ori. Specifične sekvence odgovome za prepoznavanje i vezivanje kompleksa ORC, kao i mehanizam kojim ORC selektuje orisekvence još uvek nisu poznati. Jedna od malobrojnih zajedničkih karakteristika mesta uključenih u vezivanje inicijacionog proteina jeste visok sadržaj AT bp i kratkih nizova (dA)-(dT) što ukazuje na mogućnost da ori-sekvence obrazuju sinonimne stmkture koje odreduju specifičnost vezivanja inicijacionog proteina. Radi testiranja ove pretpostavke, analizirana je struktura i oblik humane ori-sekvence lamin B2 i detaljno ispitana DNK vezivna specifičnost proteina HsOrc4. Ovaj protein predstavlja jednu od subjedinica kompleksa ORC i pokazuje istu DNK-vezivnu aktivnost kao i kompleks ORC. Utvrđeno je da ori-sekvenca, pod uslovima neutralnog pH, niske ili umerene jonske jačine i u prisustvu jona Mg2+ zauzima altemativnu formu koju karakteriše prisustvo denaturisanog regiona, okceta, i povećana elektroforetska pokretljivost u nativnoj PAGE. Stabilno prisustvo denaturisanog regiona u okviru fragmenta DNK omogućeno je formiranjem Hoogsteen-ovih vodoničnih veza izmedu centralnih pirimidina okceta i adenina jednog od kratkih nizova (dA)-(dT). Kao rezultat ove interakcije nastaje dvolančana petlja koja u osnovi sadrži intramolekulski tripleks. Struktura intramolekulskog tripleksa formira se u regionu koji interaguje sa inicijacionim proteinom i može predstavljati jedan od elemenata odgovornih za prepoznavanje i vezivanje ovog proteina. U eksperimentima direktnog vezivanja proteina HsOrc4 utvrdeno je da on ne prepoznaje kratke nizove (dA)-(dT) i (dA), dok se za (dT) slabo vezuje. Najveći afmitet protein je pokazivao za trolančane strukture tipa TAT. Kompeticija jedno-, dvo- i trolančanih molekula DNK vezivanju HsOrc4 za ori-sekvencu pokazala je da protein razlikuje trolančanu strukturu od jedno- i dvolančane i specifično se vezuje za nju. Sudeći po efikasnosti kompeticije, trolančana DNK bila je veoma slična prirodnim vezivnim mestima proteina HsOrc4. Svojstvo ori-sekvence da formira intramolekulski tripleks i specifično vezivanje proteina HsOrc4 za tripleks sugeriše da trolančana struktura može predstavljati deo mehanizma kojim inicijacioni protein prepoznaje i selektuje mesta inicijacije replikacije." ]
[ "In complex eukaryotes DNA replication is initiated by binding of origin recognition complexes (ORCs) to specific genomic sites called origins of replication. Consensus sequence required for this event and the mechanism by which ORC is localized to origins of replication remain poorly understood. General features of genomic regions involved in initiator protein binding are AT-richness and frequent occurrence of short (dA)-(dT) runs. Such distribution of A and T residues opens a possibility that origins of replication build mutually equivalent unorthodox structures which are recognised by initiation protein. In order to test this hypothesis, a study of structure and shape of the human lamin B2 origin was performed. DNA binding activity of protein HsOrc4, one of ORCs subunits that exhibited similar DNA binding properties as the whole complex, was also tested. It was shown that, at neutral pH, low or moderate ionic strength and in presence of Mg\" ions, lamin B2 ongm adopted alternative helical form, characterized by a single unpaired region and faster migration in native polyacrylamide geis. It was proposed that these properties reflected the ability of origin DNA to form double stranded loop with intramolecular triplex in its base. Triplex was kept together by Hoogsteen hydrogen bonding between Central pyrimidines of unpaired region and complementary double stranded sequence. Since intramolecular noncanonical structure formed in origin sequences protected by ORC in vivo and in vitro, it could represent the element responsible for site-specific ORC binding. In order to test this notion, binding specificity of HsOrc4 was tested in direct and competition DNA binding experiments. The protein did not recognize (dA) or (dA)-(dT), but it exhibited very low affmity for (dT) and a very high affmity for TAT triplex. Consistent with that, triple stranded DNA competed very well with origin DNA for HsOrc4 binding, whereas single or double stranded DNA exhibited much less significant competitive effect. As judged by its competitive efficiency, triple stranded DNA was very similar to naturally ocuring DNA binding sites of HsOrc4. In conclusion, formation o f intramolecular triplex within origin DNA and its specific recognition by HsOrc4 suggest that triple stranded structure might play a role in selection of eukaryotic origins of replication." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "" ]
[ "" ]
[ "Interakcija inicijacionog proteina ORC sa replikatorom regiona lamin B2", "Interaction of initiation protein Orc with lamin B2 replication region" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Кушић, Јелена; Интеракција иницијационог протеина ОРЦ са репликатором региона ламин Б2; Интеракција иницијационог протеина ОРЦ са репликатором региона ламин Б2;
[ "https://nardus.mpn.gov.rs/bitstream/id/1597/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1597/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2171" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/10259
123456789-10259.pdf
yes
yes
https://fedorabg.bg.ac.rs/fedora/get/o:18973/bdef:Content/download
no
Prenatalna dijagnostika cistične fibroze primenom metoda rekombinantne DNK
Prenatal diagnostics of cystic fibrosis by DNA analysis
[ "Savić, Ana" ]
[ "Sretenović, Zoran", "Garzičić, Branimir" ]
Radojković, Dragica
2018-12-10T11:45:15
[ "2018-12-10T11:45:15", "2020-07-03T08:06:48" ]
1997
[ "https://nardus.mpn.gov.rs/handle/123456789/10259", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6312", "https://fedorabg.bg.ac.rs/fedora/get/o:18973/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=18352399" ]
[ "Cistična fibroza je najčešće autozomno recesivno oboljenje, koje se u opulaciji belaca javlja saučestalošću od 1/2000 do 1/4000 novorođenčadi. Oboljevaju osobe kod kojih je mutirani CFTR gen prisutan u homozigotnom stanju. S obzirom na težinu ove bolesti. kao i visok rizik za rađanje obolelog deteta (1 :4) kod roditelja koji su heterozigotni nosioci mutiranog gena, prenatalna dijagnostika je od ključnog značaja. U okviru ovog istraživanja analizirano je 105 visokorizičnih porodica, sa ukupno 325 članova. Cilj analiza bio je utvrđivanje informativnosti ovih porodica za dijagnostiku cistične fibroze u narednoj trudnoći. Sprovođena je analiza na prisustvo mutacija u CFFR genu (ΔF508, G542X, G551D, i R553X) bilo direktno, bilo na osnovu polimorfizama dužina restrikcionih fragmenata (RFLP). Ovi podaci su poslužili i za određivanje učestalosti određenih mutacija u našoj populaciji, što je neophodna priprema za genetičko skrinovanje. Od analiziranih porodica 49,5% je bilo informativno, 32,4% poluinformativno, a 18,1% neinformativno za direktnu mutacionu analizu na prisustvo najčešće, ΔF508 mutacije. Od porodica neinformativnih za direktnu mutacionu analizu, 82.9% je bilo potpuno informativno za RFLP analizu. Od analiziranih porodica, 1/3 se javila na prenatalnu dijagnozu u narednoj trudnoći. U ispitivanoj populaciji porodica sa rizikom za rađanje deteta sa cističnom fibrozom, sa barem jednim rođenim obolelim detetom, utvrđeno je da je ΔF508 mutacija zastupljena sa frekvencijom od 67.2%, G542X sa 6.4%, G551D sa 0.6%, dok mutacija R553X nije detektovana. Zbirna učestalost ovih mutacija (73.4%) nije dovoljna za populaciono skrinovanje na mutacije u CFTR genu, u našoj populaciji. Na osnovu sprovedenih istraživanja predlaže se pristup u prenatalnoj dijagnozi i genetičkom savetovanju rizičnih porodica. koji uključuje skrining rizičnih porodica na prisustvo ΔF508 mutacije. Za informativne porodice, preporučuje se prenatalna dijagnoza direktnom detekcijom ove mutacije u narednoj trudnoći. U neinformativnim ili poluinformativnim porodicama sprovodi se RFLP analiza, i porodicama za koje se utvrdi da su informativne preporučuje se prenatalna dijagnoza RFLP analizom u narednoj trudnoći. dok se za neinformativne porodice preporučuje prenatalna dijagnostika ove bolesti određivanjem nivoa mikroviiarnih enzima u amnionskoj tečnosti." ]
[ "Cystic fibrosis is one of the most common autosomal recessive diseases (from 1 in 2000 to 1 in 4000 live births gives rise to an affected child). Affected persons are homozygous for the mutated CFTR gene. Regarding the severity of this disease, and a high risk (1/4) of couples heterozygous for the mutated gene to have an affected child, it is essential to perform prenatal diagnostics. In this study, 105 (325 members) high risk families were screened for the presence of mutated CFTR gene. The purpose of this study was to detect families informative for diagnostics of cystic fibrosis in further pregnancies. Both direct mutation analysis (for the presence of ΔF508, G542X, G551D and R553X) and indirect molecular genetic analysis for the presence of mutated CFTR gene (RFLPs) were performed. These data gave us too, the frequency of certain mutations in Yugoslav population which is necessary initial step for planning the population genetic screening program. Among analyzed families, 49.5% were fully informative, 32.4% partly informative, and 18.1% noninformative, for direct detection of the most common ΔF508 mutation The rest of the families were analyzed by RFLPs, and 82.9% were fully informative for RFLPs analysis in the next pregnancies. In studied families, at a known risk for cystic fibrosis, having at least one affected child, the frequency of ΔF508 was 67.2%, G542X 6.4%, G551D 0.6%, while R553X was not detected. Cumulative frequency of these mutations (73.4%) is not enough for establishing the population screening program for mutations within CFTR gene in Yugoslav population. As a final result of our study, we propose a proper approach in prenatal diagnosis and genetic counseling in cystic fibrosis in high risk families. The first step is the screening for the ΔF508. For informative families, prenatal diagnosis in the next pregnancy is recommended. In families partly informative or noninformative for the presence of the ΔF508, RFLPs analysis should be performed. In families, informative for RFLPs analysis, the prenatal diagnosis using this method is recommended in the next pregnancies. ln noninformative families, the microvilar enzyme testing inbthe amniotic fluid is the recommended method of prenatal diagnosis of cystic fibrosis" ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "" ]
Универзитет у Београду
[ "цистична фиброза", "пренатална дијагноза", "мутациона анализа" ]
[ "cystic fibrosis", "prenatal diagnosis", "mutation analysis" ]
[ "Prenatalna dijagnostika cistične fibroze primenom metoda rekombinantne DNK" ]
[ "Prenatal diagnostics of cystic fibrosis by DNA analysis" ]
doctoralThesis
[ "ARR" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1455/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1455/Disertacija.pdf", "https://fedorabg.bg.ac.rs/fedora/get/o:18973/bdef:Content/download" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10259" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/2084
123456789-2084.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:5404/bdef:Content/download
no
Ekspresija antioksidativnih enzima i transkripcionog faktora Nrf2 kod pacijentkinja sa benigno, premaligno i maligno transformisanim endometrijumom
Expression of antioxidant enzymes and transcription factor Nrf2 in patients with benign, premalignant and malignant endometrial transformation .
[ "Pejić, Snežana" ]
[ "Đurašević, Siniša", "Pajović, Snežana B.", "Saičić, Zorica S." ]
Todorović, Ana U.
2016-01-05T11:46:43
[ "2016-01-05T11:46:43", "2020-07-03T08:09:21" ]
2013-05-21
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=170", "https://nardus.mpn.gov.rs/handle/123456789/2084", "https://fedorabg.bg.ac.rs/fedora/get/o:5404/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024570802" ]
[ "Tokom čitavog životnog ciklusa aerobni organizmi su izloženi brojnim endogenim i egzogenim faktorima koji indukuju povećanje produkcije ROS-a. Prisutni u fiziološkim koncentracijama, reaktivni molekuli ROS-a imaju značajnu ulogu u važnim ćelijskim procesima kao što su regulacija signalnih kaskada i genske ekspresije. U visokim koncentracijama ROS mogu oksidovati ćelijske proteine, lipide i DNK i time dovesti do promena strukture i funkcije, oštećenja, pa i smrti ćelije. Kada koncentracija slobodnih radikala premaši fiziološki nivo, smatra se da se ćelija nalazi u stanju oksidativnog stresa. Kako bi sprečili nastanak i umanjili posledice ove vrste stresa, živi organizmi su razvili moćan antioksidativni sistem zaštite (AOS). Ovaj sistem uključuje seriju mehanizama kojima se nivo slobodnih radikala održava u uskom opsegu između fiziološke i toksične koncentracije. Najvažnije enzimske komponente AOS su: superoksid dismutaze (SOD), katalaza (CAT), glutation peroksidaza (GPx) i glutation reduktaza (GR). Ovi enzimi uklanjaju višak ROS-a i učestvuju u održavanju nivoa redukovanog glutationa, čime obezbeđuju očuvanje osnovnih životnih funkcija i sprečavaju nastanak oštećenja i bolesti ćelije. Zbog tako važne uloge AOS, regulacija funkcionalne ekspresije njegovih komponenti je naročito značajna za fiziološke i patološke procese u aerobnim organizmima. Jedan od najvažnijih regulatornih molekula u tom sistemu je Nrf2 (NF-E2 related factor 2). Nrf2 je transkripcioni faktor koji indukuje ekspresiju mnogih citoprotektivnih proteina uključujući i antioksidativne enzime, zbog čega ima značajnu ulogu u regulaciji oksidativnog stresa. Savremena istraživanja na različitim model-sistemima ukazuju na povezanost oksidativnog stresa i kancerogeneze. Razvoj kancera je višestepeni proces koji se razvija kroz tri faze: inicijaciju, promociju i progresiju, a oksidativni stres je povezan sa svakim od ova tri stadijuma transformacije ćelije. Osim toga i sam antioksidativni profil je u ćelijama kancera izmenjen u odnosu na zdrave, normalne ćelije, pa ovi molekuli mogu biti i značajni biomarkeri u dijagnostici, proceni rizika i stepena kancerogeneze..." ]
[ "During the life cycle aerobic organisms are exposed to a number of endogenous and exogenous sources of ROS. Present in low to moderate concentrations these reactive molecules play an important role in many physiological processes such as regulation of signaling cascades and gene expression. In high concentrations, ROS can oxidize cellular proteins, lipids and DNA, causing changes in structure and function, damage and even cell death. When the concentration of free radicals exceeds the physiological level, a cell is said to be in a state of oxidative stress. To prevent the onset and reduce the consequences of oxidative stress, living organisms have developed powerful antioxidant system (AOS). This system includes a set of mechanisms to maintain the level of free radicals in the narrow range between physiological and toxic concentrations. The most important enzyme components in AOS are: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR). These enzymes are involved in the removal of ROS and participate in maintaining level of reduced glutathione, thus ensuring the preservation of physiological functions and inhibit the development of cell damage and disease. Because of such an important role of AOS, the regulation of the functional expression of its components is of particular importance for physiological and pathological processes in aerobic organisms. One of the most important regulatory molecules in this system is Nrf2 (NF-E2 related factor 2). Nrf2 is a transcription factor that induces expression of many cytoprotective proteins including antioxidant enzymes, and therefore play an important role in the regulation of oxidative stress. Previous studies have clearly demonstrated a link between oxidative stress and carcinogenesis. Cancer is a multistage process that develops over three stages: initiation, promotion and progression and oxidative stress is associated with each of them. Moreover, antioxidant profile is altered in cancer cells compared with healthy, normal tissue, so these molecules may be important biomarkers in the assessment of risk and the degree of carcinogenesis" ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173041/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Superoksid dismutaza", "katalaza", "glutation peroksidaza", "glutation reduktaza", "Nrf2", "kancer endometrijuma", "polip", "miom", "hiperplazija", "adenokarciinom" ]
[ "Superoxide dismutase", "catalase", "glutathione peroxidase", "glutathione reductase", "Nrf2", "endometrial cancer", "polyp", "myoma", "hyperplasia", "adenocarcinoma" ]
[ "Ekspresija antioksidativnih enzima i transkripcionog faktora Nrf2 kod pacijentkinja sa benigno, premaligno i maligno transformisanim endometrijumom", "Expression of antioxidant enzymes and transcription factor Nrf2 in patients with benign, premalignant and malignant endometrial transformation ." ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Пејић, Снежана; Пајовић, Снежана Б.; Ђурашевић, Синиша; Саичић, Зорица С.; Тодоровић, Aна У.; Експресија антиоксидативних ензима и транскрипционог фактора Нрф2 код пацијенткиња са бенигно, премалигно и малигно трансформисаним ендометријумом; Експресија антиоксидативних ензима и транскрипционог фактора Нрф2 код пацијенткиња са бенигно, премалигно и малигно трансформисаним ендометријумом;
[ "https://nardus.mpn.gov.rs/bitstream/id/2070/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2070/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2084" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/9103
123456789-9103.pdf
yes
no
https://nardus.mpn.gov.rs/bitstream/id/39087/Disertacija.pdf
no
Optimizacija i karakterizacija elektrolita na bazi jonskih tečnosti pogodnih za litijum jonske baterije
Optimization and characterization of ionic liquid based electrolytes for Li-ion batteries
[ "Gadžurić, Slobodan" ]
[ "Vaštag, Đenđi", "Gadžurić, Slobodan", "Vraneš, Milan", "Cvjetićanin, Nikola", "Marija-Bešter, Rogač" ]
Zec, Nebojša
2018-01-12T13:44:51
[ "2018-01-12T13:44:51", "2020-07-03T13:47:09" ]
2017-11-08
[ "https://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija150460451728711.pdf?controlNumber=(BISIS)104989&fileName=150460451728711.pdf&id=10463&source=NaRDuS&language=sr", "https://nardus.mpn.gov.rs/handle/123456789/9103", "https://www.cris.uns.ac.rs/record.jsf?recordId=104989&source=NaRDuS&language=sr", "https://www.cris.uns.ac.rs/DownloadFileServlet/IzvestajKomisije150460457497229.pdf?controlNumber=(BISIS)104989&fileName=150460457497229.pdf&id=10464&source=NaRDuS&language=sr" ]
[ "U ovoj doktorskoj disertaciji ispitivani su elektroliti na bazi jonskih tečnosti pogodni za primenu u litijum jonskim baterijama. Fizičko-hemijska svojstva binarnih smeša jonskih tečnosti sa dicijanamidnim i bis(trifluorometilsulfonil)imidnim anjonima i molekulskih rastvarača ispitana su u celom opsegu molskih udela i na različitim temperaturama. Na osnovu izmerenih gustina, viskoznosti i električne provodljivosti izračunati su različiti fizičko hemijski parametri i diskutavne interakcije između komponenata smeša. Ispitana je termička i elektrohemijska stabilnost odabranih elektrolita. Dodatkom litijumove soli u odabrane binarne smeše dobijeni su ternarni sistemi koji su okarakterisani u zavisnoti od koncentracije litijumove soli. Odabrani elektroliti upotrebljeni su za ispitivanje performansi litijum jonske ćelije sa anatas TiO2 nanotubularnim elektrodama.Cikličnom voltametrijom i galvanostatskim cikliranjem su ispitane performanse ćelije u toku 150 ciklusa punjenja i pražnjenja. Na osnovu ciklovoltametrijskih merenja izračunati su koeficijenti difuzije i energija aktivacije za difuziju." ]
[ "In this doctoral dissertation, Ion liquid-based electrolytes were tested for use in lithium-ion batteries. The physicochemical properties of binary mixtures of ionic liquids with dicyanamide and bis (trifluoromethylsulfonyl) imide anions and molecular solvents were examined throughout the range of molar proportions and at different temperatures. Based on the measured densities, viscosity and electrical conductivity, various physical chemical parameters and discrete interactions between the components of the mixture are calculated. Thermal and electrochemical stability of selected electrolytes was examined. By addition of lithium salt to the selected binary mixtures, ternary systems were characterized which were characterized by the concentration of lithium salt. The selected electrolytes were used to test the performance of the lithium-ion cell with anatomic TiO2 nanotubular electrodes. Cyclic voltammetry and galvanostatic cycling tested the cell's performance during the 150 charge and discharge cycles. Based on cyclotoltametric measurements, the diffusion coefficients and activation energies for diffusion were calculated." ]
sr (latin script)
Универзитет у Новом Саду, Природно-математички факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172012/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Новом Саду
[ "Jonske tečnosti", "Litijum jonske baterije", "fizičko-hemijska svojstva" ]
[ "Ionic liquids", "Li-ion batteries", "physicochemical properties" ]
[ "Optimizacija i karakterizacija elektrolita na bazi jonskih tečnosti pogodnih za litijum jonske baterije" ]
[ "Optimization and characterization of ionic liquid based electrolytes for Li-ion batteries" ]
doctoralThesis
[ "BY-NC-ND" ]
Гаджурић, Слободан; Ваштаг, Ђенђи; Гаджурић, Слободан; Вранеш, Милан; Цвјетићанин, Никола; Марија-Бештер, Рогач; Зец, Небојша; Оптимизација и карактеризација електролита на бази јонских течности погодних за литијум јонске батерије; Оптимизација и карактеризација електролита на бази јонских течности погодних за литијум јонске батерије;
[ "https://nardus.mpn.gov.rs/bitstream/id/39088/IzvestajKomisije.pdf", "http://nardus.mpn.gov.rs/bitstream/id/39087/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/39088/IzvestajKomisije.pdf", "https://nardus.mpn.gov.rs/bitstream/id/39087/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_9103" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/2087
123456789-2087.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:2300/bdef:Content/download
no
Molekularna i morfološka karakterizacija vrsta iz kompleksa Praon dorsale- yomenae (Hymenoptera, Braconidae)
Molecular and morphological characterization in Praon dorsale- yomenae species complex (Hymenoptera, Braconidae)
[ "Tomanović, Željko" ]
[ "Mitrović, Milana", "Ivanović, Ana", "Blesić, Bela", "Vorburger, Christoph" ]
Mitrovski Bogdanović, Ana S.
2016-01-05T11:46:43
[ "2016-01-05T11:46:43", "2020-07-03T08:09:24" ]
2012-07-21
[ "https://nardus.mpn.gov.rs/handle/123456789/2087", "http://eteze.bg.ac.rs/application/showtheses?thesesId=30", "https://fedorabg.bg.ac.rs/fedora/get/o:2300/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024495538" ]
[ "Molekularna i morfološka varijabilnost unutar kompleksa vrsta Praon dorsale-yomenae analizirana je primenom tri nezavisne metode: “tradicionalne” morfometrije, geometrijske morfometrije i analize citohrom oksidaza I sekvenci mitohondrijalne DNK i 28S D2 gena nuklearne DNK. Za morfometrijske analize korišćena je 421 jedinka (270 ţenki i 151 muţjak) sakupljena sa preko 30 razliĉitih afidnih domaćina širom Palearktika. Molekularne analize su obuhvatile 47 jedinki u asocijaciji sa razliĉitim domaćinima. Primenom diskriminantne kanonijske analize utvrĊeno je koji su karakteri dovoljno informativni za morfološku diferencijaciju analiziranih taksona. CVA analizom su prikazane razlike u obliku krila izmeĊu vrsta. Varijabilnost oblika krila meĊu biotipovima analizirana je primenom PCA. Pokazano je da postoji znaĉajan efekat domaćina na varijabilnost oblika krila meĊu biotipovima vrsta P. dorsale i P. longicorne, dok je meĊu biotipovima vrste P. yomenae utvrĊen znaĉajan efekat geografske distribucije. Analize oblika krila ukazale su na to da se vrste „dorsale-yomenae“ kompleksa mogu morfološki razlikovati na osnovu duţine radijalnog i povratnog nerva. Geometrijska morfometrija primenjena u cilju vizualizacije suptilnih razlika u obliku krila pokazala je veću osetljivost u razdvajanju analiziranih taksona „dorsale-yomenae“ grupe u odnosu na „tradicionalnu” morfometriju. Ispitali smo validnost prethodno korišćenih morfoloških karaktera u identifikaciji „dorsale-yomenae“ grupe. TakoĊe smo ukazali na nove karaktere koji se mogu koristiti u kljuĉevima za identifikaciju. UtvrĊeni su razliĉiti obrasci obojenosti krilne nervature unutar kompleksa vrsta „dorsale-yomenae“. Na osnovu rezultata molekularnih analiza potvrĊen je status vrsta P. longicorne, P. dorsale, P. yomenae i P. volucre. UtvrĊeni su novi domaćini/asocijacije za vrstu P. dorsale. Otkrivena je nova vrsta za nauku koja parazitira vaš Staticobium limonii na Limonium angustifolium. Vrsta P. megourae nije dobila molekularnu potvrdu pa su za rasvetljavanja statusa ovog taksona potrebna dodatna istraţivanja." ]
[ "Molecular and morphological variability of Praon dorsale-yomenae species complex was analyzed using the three independent methods: “traditional” morphometrics, geometric morphometrics and DNA sequence analysis of two gene regions (COI mtDNA and 28S D2). For morphometric analysis we used 421 specimens (270 females and 151 males) reared from about 30 different species of aphid hosts throughout Palaearctic. Molecular analysis included 47 specimens in association with different aphid hosts. Discriminant Canonical Analysis was performed in order to determine which characters were informative for morphological diferentiation of analyzed taxa. To explore differences in wing shape between species we performed Canonical Variate Analysis. Variation in wing shape among biotypes was investigated using Principal Component Analysis. We found that great variablity in wing shape of P. dorsale and P. longicorne biotypes was influenced by the host, while great effect of geographical distribution was noticed between P. yomenae biotypes. Analysis of wing shape demonstrated that „dorsale-yomenae“ species can be morphologically discriminated on the basis of length of forewing radial and recurrent vein. Geometric morphometrics applied to visualize subtle shape differences had more sensitivity than „traditional“ morphometrics in distiguishing taxa within „dorsale-yomenae“ group. We tested the validity of morphological characteristics previously used for identification in „dorsale-yomenae“ group, also indicating a new characters that could be used in identification keys. Different colour patterns of wing venation in „dorsale-yomenae“ species complex were found. On the basis of molecular results P. longicorne, P. dorsale, P. yomenae and P. volucre was confirmed as separate species. We presented a new tritrophic association for P. dorsale. A new parasitoid species from Staticobium limonii infesting Limonium angustifolium was described. P. megourae was not confirmed as separate species using molecular markers, thus the clarification of its status requires further research." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43001/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc/4.0/" ]
Универзитет у Београду
[ "„Dorsale-yomenae“ kompleks vrsta", "geometrijska morfometrija", "tradicionalna morfometrija", "COI mtDNK", "28S D2" ]
[ "„Dorsale-yomenae“ species complex", "geometric morphometry", "traditional morphometry", "COI mtDNA", "28S D2" ]
[ "Molekularna i morfološka karakterizacija vrsta iz kompleksa Praon dorsale- yomenae (Hymenoptera, Braconidae)", "Molecular and morphological characterization in Praon dorsale- yomenae species complex (Hymenoptera, Braconidae)" ]
[ "" ]
doctoralThesis
[ "BY-NC" ]
Томановић, Жељко; Митровић, Милана; Ворбургер, Цхристопх; Блесић, Бела; Ивановић, Aна; Митровски Богдановић, Aна С.;
[ "https://nardus.mpn.gov.rs/bitstream/id/2082/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2082/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2087" ]
10.2298/bg20120721mitrovskibogdanovic
[ "" ]
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[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/11469
123456789-11469.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:20215/bdef:Content/download
no
Ispitivanje probiotičkih i imunomodularnih karakteristika prirodnih izolata enterokoka u modelima in vitro i in vivo
Examination of probiotic and immunomodulatory characteristics of natural isolates of enterococci on in vitro and in vivo models
[ "Veljović, Katarina" ]
[ "Jovčić, Branko", "Đokić, Jelena", "Veljović, Katarina", "Jovčić, Branko" ]
Popović, Nikola D.
2019-10-31T09:33:00
[ "2019-10-31T09:33:00", "2020-07-03T08:07:45" ]
2019-07-05
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6894", "https://nardus.mpn.gov.rs/handle/123456789/11469", "https://fedorabg.bg.ac.rs/fedora/get/o:20215/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025226930" ]
[ "Enterokoke pripadaju grupi mleĉnokiselinskih bakterija koje su široko rasprostranjene u prirodi. Predominantno se nalaze u gastrointestinalnom traktu životinja, od insekata do ĉoveka i preko fecesa se oslobaĊaju u spoljašnju sredinu, nakon ĉega naseljavaju razliĉita staništa. Imaju kontroverzni status zbog uloge u bolniĉkim infekcijama i širenja gena odgovornih za rezistenciju na antibiotike. S druge strane, neki sojevi se koriste kao probiotici za životinje i ĉoveka, kao i starter kulture u mlekarskoj industriji. Da bi se enterokoke koristile kao probiotici neophodno je da budu bezbedne za korišćenje, da nemaju hemolitiĉku i želatinoznu aktivnost i da nisu otporne na antibiotike. Enterokoke sintetišu antimikrobne proteine, bakteriocine, i na taj naĉin regulišu brojnost drugih bakterija i modulišu odgovor domaćina na infekciju. One mogu da sintetišu i biomolekule koji interaguju sa ćelijama domaćina i na taj naĉin mogu da menjaju razliĉite signalne puteve unutar ćelija domaćina. Biomolekuli sintetisani od strane probiotiĉkih enterokoka nazivaju se postbiotici. Primena postbiotika predstavlja bezbednu alternativu korišćenja enterokoka u biomedicini. Ova doktorska disertacija imala ja za cilj da ispita probiotiĉka i imunomodulatorna svojstva prirodnih sojeva enterokoka izolovanih iz fermentisanih mleĉnih proizvoda sa podruĉja Zapadnog Balkana. Definisana su tri cilja: 1. da se pronaĊu sojevi bezbedni za korišćenje u biomedicini; 2. da se ispitaju probiotiĉke karakteristike tih sojeva u modelima in vitro; i 3. da se ispita imunomodulatorni efekat odabranog soja u modelima in vitro i in vivo. U cilju ispitivanja bezbedne upotrebe enterokoka kao probiotika testirano je ukupno 75 sojeva iz fermentisanih mleĉnih proizvoda koje pripadaju vrstama: Enterococcus durans (50 sojeva), Enterococcus faecium (15 sojeva), Enterococcus faecalis (6 sojeva), Enterococcus italicus (3 soja) i Enterococcus hirae (1 soj). Hemolitiĉku aktivnost pokazalo je 18,7% (14/75), dok je želatinaznu aktivnost imalo 6,7% (5/75) testiranih sojeva. Na osnovu rezultata testa mikrodilucije pokazana je visoka uĉestalost rezistencije na ciprofloksacin 48,2% (27/56), dok su u manjoj meri testirani sojevi rezistentni na gentamicin 10,7% (6/56). Na osnovu rezultata sposobnosti formiranja biofilma u primenjenim uslovima, devet sojeva nema sposobnost formiranja biofilma, 11 sojeva ima mogućnost formiranja slabog biofilma, BGGO9-28 ima sposobnost formiranja jakog biofilma, dok sojevi BGTRK4-42 i BGZLM1-5 pokazuju veoma jaku sposobnost formiranja..." ]
[ "are predominantly found in the gastrointestinal tract of animals, from insects to humans and through faeces they are released into the environment, where they colonize different habitats. They have a controversial status according to the role as causative agents of hospital infections and the spread of genetic determinants of antibiotic resistance. In contrary, some strains have been used used as probiotics for animals and humans, as well as a starter cultures in the dairy industry. In order to use enterococci as probiotics, it is necessary to determine their safety, i.e. absence of hemolytic and gelatinase activity and susceptiblity to clinicaly relevant antibiotics. Enterococci synthesize antimicrobial proteins, bacteriocins, by which way they regulate the number of other bacteria and modulate the host response to the infection. They can also synthesize biomolecules that interact with the host cells, and change the different signalling pathways within host cells. Biomolecules synthesized by probiotic enterococci are called postbiotics. The use of postbiotic represents a safety alternative to the enterococci application in biomedicine. The aim of this dissertation was examination of probiotic and immunomodulatory characteristics of enterococci isolated from fermented dairy products from the Western Balkans countries. Three objectives were defined: 1. to find strains safe for use in biomedicine; 2. to examine the probiotic characteristics on in vitro models, and 3. to examine the immunomodulatory effect of the selected strain on in vitro and in vivo models. In order to analyse the safe use of enterococci as a probiotics, a total of 75 isolates from fermented dairy products were tested: Enterococcus durans (50 isolates), En. faecium (15 isolates), En. faecalis (6 isolates), En. italicus (3 isolates) and En. hirae (1 isolate). 18.7% (14/75) strains showed hemolytic activity, while 6.7% (5/75) had gelatinase activity. Based on the results of the microdilution test, 48.2% (27/56) of strains were ciprofloxacin resistance, while 10.7% (6/56) strains were resistant to gentamicin. According to the analysis of selected enterococci genomes, high incidence of adhesin encoding genes could be noticed. It was found that 30.4% of strains have three genes encoding different virulence factors, 21.7% of strains having two or four genes, 17.4% having five genes, and 4.3% of studied enterococci containing 6 or 7 genes which encode virulence factors, while the presence or absence of gene within the fsr operon varies from the analyzed strains..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41004/RS//", "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173019/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Enterococcus", "probiotik", "toplotom ubijen", "Listeria monocytogenes infekcija", "imunomodulacija", "EAE" ]
[ "Enterococcus", "probiotic", "heat-killed", "Listeria monocytogenes infection", "immunomodulatory", "EAE" ]
[ "Ispitivanje probiotičkih i imunomodularnih karakteristika prirodnih izolata enterokoka u modelima in vitro i in vivo" ]
[ "Examination of probiotic and immunomodulatory characteristics of natural isolates of enterococci on in vitro and in vivo models" ]
doctoralThesis
[ "BY-NC-ND" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1680/IzvestajKomisije21052.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1679/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1680/IzvestajKomisije21052.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1679/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_11469" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/17729
123456789-17729.pdf
yes
yes
no
Evaluacija modela tais za utvrđivanje predispozicija zaposlenih za rad u ugostiteljstvu
[ "Čerović, Slobodan" ]
[ "Popesku, Jovan", "Vukosav, Svetlana" ]
Mitrašinović, Ivana
2020-12-28T14:49:04
[ "2020-12-28T14:49:04" ]
2020-11-26
[ "https://singipedia.singidunum.ac.rs/izdanje/43218-evaluacija-modela-tais-za-utvrdjivanje-predispozicija-zaposlenih-za-rad-u-ugostiteljstvu", "https://nardus.mpn.gov.rs/handle/123456789/17729" ]
[ "" ]
[ "" ]
Универзитет Сингидунум, Студије при универзитету
[ "" ]
[ "openAccess" ]
[ "" ]
Универзитет Сингидунум
[ "" ]
[ "" ]
[ "Evaluacija modela tais za utvrđivanje predispozicija zaposlenih za rad u ugostiteljstvu" ]
[ "" ]
doctoralThesis
[ "ARR" ]
Черовић, Слободан; Вукосав, Светлана; Попеску, Јован; Митрашиновић, Ивана; Евалуација модела таис за утврђивање предиспозиција запослених за рад у угоститељству; Евалуација модела таис за утврђивање предиспозиција запослених за рад у угоститељству;
[ "https://nardus.mpn.gov.rs/bitstream/id/67562/bitstream_67562.pdf", "https://nardus.mpn.gov.rs/bitstream/id/67561/bitstream_67561.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_17729" ]
sr
[ "Menadžment i biznis" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/2157
123456789-2157.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:7886/bdef:Content/download
no
Efekti estradiola na regulaciju natrijum-kalijumove pumpe i morfologiju srca normalno uhranjenih i gojaznih pacova
The effects of estradiol on the regulation of sodium-potassium pump and heart morphology in normally fed and obese rat
[ "Isenović, Esma R." ]
[ "Đorđević, Jelena", "Labudović-Borović, Milica", "Bajić, Vladan" ]
Obradović, Milan M.
2016-01-05T11:48:26
[ "2016-01-05T11:48:26", "2020-07-03T08:11:20" ]
2013-12-27
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=1126", "https://nardus.mpn.gov.rs/handle/123456789/2157", "https://fedorabg.bg.ac.rs/fedora/get/o:7886/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024653234" ]
[ "Hormon estradiol, pored svojih efekata koje ostvaruje na reprodukciju i razviće, uključen je i u regulaciju metaboličkih procesa u različitim organima, uključujući i srce. Pored sistemskog efekta koje estradiol ostvaruje na kardiovaskularni sistem zapažen je, ali i nedovoljno razjašnjen, uticaj estradiola na srce i kardiomiocite. U literaturnim podacima koji se odnose na efekte estradiola na morfologiju srca uglavnom su izučavani efekti estradiola u patofiziološkim stanjima hipertenzije i traume, dok je uloga estradiola na morfologiju srca u patofiziološkim stanjima gojaznost i rezistencije na insulin (IR) još uvek nedovoljno izučena. Estradiol svoje efekte ostvaruje genomskim i negenomskim signalnim putevima posredstvom specifičnih receptora za estrogen (ER), pri čemu dolazi do aktivacije složene mreže međuzavisnih intracelularnih signalnih puteva uključujući i fosfatidilinozitol-3 kinazu (PI3K), protein kinazu B (Akt), kao i ekstracelularnim signalima regulisane kinaze 1 i 2 (ERK1/2). Natrijum-kalijum adenozin trifosfataza (Na+/K+-ATPaze) je membranski protein koji ima ulogu u održavanju elektrohemijskog gradijenta Na+ i K+ jona kroz ćelijsku membranu, a takođe ima i važnu ulogu u regulaciji tonusa glatkih mišića vaskulature, kao i regulaciji kontrakcija srca. Poremećaji u funkciji Na+/K+-ATPaze uočeni su u različitim patofiziološkim stanjima kao što su stanja hipertenzije, gojaznosti i IR. Signalni putevi koji su uključeni u estradiolom regulisanoj ekspresiji i aktivnosti Na+/K+-ATPaze uključuju i aktivaciju Akt i ERK1/2 kinaza. Stimulišući aktivnost Na+/K+-ATPaze, estradiol ostvaruje direktan uticaj na srce povećavajući kontraktilnost ćelija srca. Nedostatak podataka u literaturi o in vivo efektima estradiola na regulaciju Na+/K+-ATPaze i morfologiju srca, kao i mehanizmi kojima estradiol ostvaruje svoje efekte na ćelije srca u patofiziološkim stanjima (gojaznost i IR), ukazuju na značaj realizovanih istraživanja u okviru ove doktorske disertacije. U ovoj doktorskoj disertaciji izučavani su molekularni mehanizmi in vivo efekata estradiola na regulaciju Na+/K+-ATPaze i morfologiju srca, u koje su uključene Akt i ERK1/2 kinaze, kako u fiziološkim tako i u patofiziološkim uslovima kao što su stanja gojaznosti i IR. U eksperimentima su korišćeni adultni pacovi Wistar soja. Dve grupe pacova za ishranu su koristile standardnu laboratorijsku hranu za pacove (normalno uhranjeni pacovi), dok su druge dve grupe hranjene standardnom laboratorijskom hranom obogaćenom sa 42% masti (gojazni pacovi)..." ]
[ "Hormone estradiol is also involved in the regulation of metabolic processes in various organs, including the heart. The systemic effects of estradiol on cardiovascular system, was observed, but the effect of estradiol on the heart still is not known in detail. The effects of estradiol on the morphology of the heart are mainly studied the effects of estradiol in pathophysiological conditions of hypertension and trauma, whereas the role of estradiol on the morphology of the heart in pathophysiological conditions such are obesity and insulin resistance (IR) is still insufficiently explored. Estradiol exert its effect by nongenomic and genomic signaling pathways, through specific receptors for estrogen (ER), via the activation of a complex network of dependent intracellular signaling pathways including phosphatidylinositol 3 - kinase (PI3K), protein kinase B (Akt) and extracellular signal-regulated kinases 1 and 2 (ERK1/2). Sodium-potassium adenosine triphosphatase (Na+/K+-ATPase ) is a membrane protein that plays a role in maintaining the electrochemical gradient of Na+ and K+ ions across the cell membrane, and also plays an important role in the regulation of muscle tone of smooth muscle vasculature , as well as in the regulation of heart contraction. Disturbances in the Na+/K+-ATPase function have been observed in various pathophysiological conditions such as hypertension, obesity and IR. Estradiol regulates Na+/K+-ATPase activity and expression via the activation of Akt and ERK1/2 signaling pathways. Stimulating the activity of the Na+/K+-ATPase, estradiol has direct effect on the heart by increasing the contractility of cardiac cells. The lack of literature data about in vivo effects of estradiol on the Na+/K+-ATPase regulation, the heart morphology, and the mechanisms by which estradiol achieves its effects on heart cells in pathophysiological conditions (obesity and IR) stresses the importance of the research within the framework of this doctoral dissertation. In this doctoral thesis, the molecular mechanisms of in vivo effects of estradiol on the regulation of Na+/K+-ATPase and the morphology of the heart, via Akt and ERK1/2 kinase, both in physiological and in pathophysiological conditions such as obesity and IR, were studied and for these experiments adult Wistar rats were used. Two groups of rats were fed with standard laboratory rats’ foods (normally fed), while the other two groups were used standard laboratory food enriched with 42% fat (obese rats)..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173033/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by/4.0/" ]
Универзитет у Београду
[ "Estradiol", "Akt", "ERK1/2", "Na+", "K+-ATPaza", "srce" ]
[ "Estradiol", "Akt", "ERK1/2", "Na+", "K+-ATPase", "heart" ]
[ "Efekti estradiola na regulaciju natrijum-kalijumove pumpe i morfologiju srca normalno uhranjenih i gojaznih pacova", "The effects of estradiol on the regulation of sodium-potassium pump and heart morphology in normally fed and obese rat" ]
[ "" ]
doctoralThesis
[ "BY" ]
Исеновић, Есма Р.; Ђорђевић, Јелена; Бајић, Владан; Лабудовић-Боровић, Милица; Обрадовић, Милан М.; Ефекти естрадиола на регулацију натријум-калијумове пумпе и морфологију срца нормално ухрањених и гојазних пацова; Ефекти естрадиола на регулацију натријум-калијумове пумпе и морфологију срца нормално ухрањених и гојазних пацова;
[ "https://nardus.mpn.gov.rs/bitstream/id/2497/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2497/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2157" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/9293
123456789-9293.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:17305/bdef:Content/download
no
Analiza promotorskih varijanti gena TGFB1 kao faktora rizika i modulatora odgovora na terapiju astme
Analysis of the TGFB1 gene promoter variants as risk factors and modulators of response to asthma therapy
[ "Nikolić, Aleksandra" ]
[ "Radojković, Dragica", "Savić-Pavićević, Dušanka", "Nikolić, Aleksandra", "Radojković, Dragica" ]
Dragičević, Sandra Z.
2018-03-28T14:04:38
[ "2018-03-28T14:04:38", "2020-07-03T08:06:37" ]
2017-12-15
[ "https://nardus.mpn.gov.rs/handle/123456789/9293", "http://eteze.bg.ac.rs/application/showtheses?thesesId=5657", "https://fedorabg.bg.ac.rs/fedora/get/o:17305/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=49916175" ]
[ "Transformišući faktor rasta beta 1 (TGFB1) je važan inflamatorni modulator i profibrotički medijator u astmi. Promene u regulaciji ekspresije TGFB1 bi mogle biti povezane sa patogenezom astme i sa adekvatnim odgovorom na terapiju. Cilj ovog rada je bio ispitivanje promotorskih varijanti gena TGFB1 kao faktora rizika za nastanak astme i modulatora odgovora na terapiju. Studija je uključila pacijente sa astmom i zdrave ispitanike kod kojih je analiziran 5' regulatorni region gena TGFB1. Detektovane su promotorske varijante -509C>T (rs1800469) i -800G>A (rs1800468) za koje asocijativnom studijom nije pokazana povezanost sa patogenezom astme, ali je uočena potencijalna povezanost između promotorske varijante -509C>T i egzacerbacija bolesti. Uticaj ove promotorske varijante na ekspresiju gena TGFB1 pod dejstvom montelukasta, leka za astmu, je ispitivan ex vivo u ćelijama indukovanog sputuma i in vitro u ćelijama BEAS 2B. Analizom ex vivo je pokazana smanjena ekspresija gena TGFB1, pri čemu je efekat bio izraženiji kod astmatičara sa genotipom -509TT (58,9%) u odnosu na astmatičare sa genotipovima -509CC (49,6%) i -509CT (31,8%). U ćelijama BEAS 2B je ekspresija gena bila smanjena za 27%. Nakon transfekcije ćelija BEAS 2B konstruktima sa promotorskim varijantama -509C ili -509T i tretmana montelukastom, zapažena je povećana aktivnost promotora gena TGFB1. Odgovor varijante -509T je bio intenzivniji i za 139,9% veći u odnosu na odgovor varijante -509C. Ova studija je pokazala da promotorska varijanta -509C>T menja ekspresiju gena TGFB1 u odgovoru na montelukast, ali da bi se njena modulatorna uloga razjasnila, u daljim istraživanjima bi trebalo analizirati i druge genetičke i negenetičke faktore." ]
[ "Transforming growth factor beta 1 (TGFB1) is an important inflammatory modulator and profibrotic mediator in asthma. The changes in regulation of TGFB1 expression could be associated with asthma pathogenesis and adequate response to therapy. The aim of this study was to investigate TGFB1 gene promoter variants as asthma risk factors and modulators of response to asthma therapy. The 5' regulatory region of the TGFB1 gene was analyzed in asthmatic patients and healthy individuals included in this study. The promoter variants -509C>T (rs1800469) and -800G>A (rs1800468) were detected and the association between these variants and asthma pathogenesis was not found. The association was observed between the presence of variant -509C>T and occurence of exacerbations in patients. The influence of -509C>T variant on TGFB1 gene expression in response to montelukast, used for asthma treatment, was further investigated ex vivo in induced sputum cells and in vitro in BEAS 2B cells. Analysis ex vivo has shown the decrease in the TGFB1 gene expression, with more pronaunced effect in asthmatics with genotype -509TT (58.9%) in comparison with -509CC (49.6%) and -509CT (31.8%) genotypes. In BEAS 2B cells the gene expression was reduced for 27%. After transfection of BEAS 2B cells with constructs carrying promoter variants -509C or -509T and treatment with montelukast the increased activity was observed for both promoter variants. The response of variant -509T was more intensive and 139,3% higher than response of variant -509C. This study showed that promoter variant -509T alters TGFB1 gene expression in response to montelukast, but to clarify its modulatory role other genetic, and nongenetic factors as well, should be taken into consideration in further studies." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173008/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "astma", "montelukast", "promotor", "terapija", "transformišući faktor rasta", "varijanta" ]
[ "asthma", "montelukast", "promoter", "therapy", "transforming growth factor", "variant" ]
[ "Analiza promotorskih varijanti gena TGFB1 kao faktora rizika i modulatora odgovora na terapiju astme" ]
[ "Analysis of the TGFB1 gene promoter variants as risk factors and modulators of response to asthma therapy" ]
doctoralThesis
[ "BY-NC-ND" ]
Николић, Aлександра; Радојковић, Драгица; Савић-Павићевић, Душанка; Николић, Aлександра; Радојковић, Драгица; Драгичевић, Сандра З.; Aнализа промоторских варијанти гена ТГФБ1 као фактора ризика и модулатора одговора на терапију астме; Aнализа промоторских варијанти гена ТГФБ1 као фактора ризика и модулатора одговора на терапију астме;
[ "https://nardus.mpn.gov.rs/bitstream/id/1415/IzvestajKomisije15775.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1414/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1414/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1415/IzvestajKomisije15775.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_9293" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/10968
123456789-10968.pdf
yes
yes
https://fedorabg.bg.ac.rs/fedora/get/o:19436/bdef:Content/download
no
Biosinteza rezervnih proteina semena heljde
Biosynthesis of Buckwheat Seed Storage Proteins
[ "Savić, Ana" ]
[ "Nešković, Mirjana", "Glišin, Vladimir" ]
Maksimović, Vesna R.
2019-04-22T11:59:00
[ "2019-04-22T11:59:00", "2020-07-03T08:07:39" ]
1997-04-18
[ "https://nardus.mpn.gov.rs/handle/123456789/10968", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6625", "https://fedorabg.bg.ac.rs/fedora/get/o:19436/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=22199311" ]
[ "Резервни протеини семена хељде интересантни су за истрживање из два разлога-то су протеини високе биолошке вредности упоредиви по аминокиселинском саставу са онима животињског порекла, а осим тога регулација експресије гена који кодирају ове протеине специфична за дефинисану фазу развића и ткиво, посебно је интересантна за фундаментална истраживања. Резервни протеини семена хељде сачињени су од три посебне протеинске фракције: 13S глобулини (легумини), mc-глобилини(вицилини) и 2S албумини . Анализа биосинтезе ових протеина обухватала је поређење производа in vitro транслације poly(A)РНК изолованих из семена хељде у различитим фазама развића са in vivo обележеним протеинима изолованим из исте фазе. Показано је да је средња фаза развића она у којој доминира синтеза реѕервних протеина што је уобичајено и код осталих биљних врста. Оно што је специфично за хељду је то да легумински тип резервног протеина није производ пост-ранслацијске обраде прекурсора, већ су компоненте кодиране посебним појединачним иРНК. Биосинтеза mc глобулина и вицилина је поредива са осталим биљним врстама Специфичност резервних протеина за семе је потврђена поређењем in vitro транслационих производа poly(A) РНК изолованих из листа, цвета и хипокотила. Осим претходног, део истраживања је био фокусиран на амализу протеина и poly(A) РНК семена хељде која је расла у медијуму дефицитарном у сумпору.Под овим условима, синтеза албумина и базних субјединица 13S глобилина била је значајно редукована." ]
[ "What makes buckwheat interesting for research is that buckwheat seed contains 10-12% proteins which, belonging to the proteins of high biological value according to their amino acid composition, similar to animal proteins. Furthermore, the regulation of gene expression of storage proteins is particularly important in fundamental research, as intricate mechanisms of gene switching in late embryogenesis and tissue specificity can be studied on those particular genes. Buckwheat seed storage proteins comprise three distinct protein fractions: 13S globulins, mc-globulins and albumins, belonging to the legumin-like, vicilin-like and 2S albumin-like storge proteins respectively. In studing the biosinthesis of these proteins, the prducts of in vitro translation of the poly(A) RNA islated from the buckwneat seed in different developmental stages were compared with the in vivo labeled proteins as well as with the proteins directly isolated from the respective developmental stages.The results obtained show that the changes in mRNAS correspond to the changes in proteins during development, and that mRNAs for storage proteins are the dominant ones in the mid-maturation stage, the finding which is common for nany other plants.However, the biosinthesis of 13S globulin differs from other legumin-like proteins in that the 13S subunit-polipeptides are not the products of post-translational processing but are durectly encoded by undividual mRNAS. The biosinthesis of mc globulins follows the usual pattern for other vicilin-like proteins. The seed specificity of storage protein gene expression was also confirmed by comparison of in vitro translation pproducts of poly(A)RNAs from seed with those from leaf, flower and hypocotil. Next, we focused our research on the analysis of the proteins and poly(A)RNAs of the buckwheat seed which was grown in a sulfur defiient medium.Under those condition, the synthesis of storage proteins-spacifically albumins and basic subunits of the 13S globulins –was significantly reduced." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "" ]
Универзитет у Београду
[ "Хељда", "резервни протеини", "генска експресија", "ткивна специфичност" ]
[ "Buckwheat", "storage proteins", "gene expression", "tissue specificity" ]
[ "Biosinteza rezervnih proteina semena heljde" ]
[ "Biosynthesis of Buckwheat Seed Storage Proteins" ]
doctoralThesis
[ "ARR" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1664/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1664/Disertacija.pdf", "https://fedorabg.bg.ac.rs/fedora/get/o:19436/bdef:Content/download" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10968" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/2188
123456789-2188.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:6214/bdef:Content/download
no
Dynamic analysis of soil-structure system using spectral element method
Динамичка анализа система тло-конструкција применом спектралних елемената.
[ "" ]
[ "" ]
Nefovska-Danilović, Marija T.
2016-01-05T11:51:58
[ "2016-01-05T11:51:58", "2020-07-03T08:28:23" ]
2013-05-14
[ "https://nardus.mpn.gov.rs/handle/123456789/2188", "http://eteze.bg.ac.rs/application/showtheses?thesesId=431", "https://fedorabg.bg.ac.rs/fedora/get/o:6214/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=43118351" ]
[ "3D numerical model for dynamic response analysis of multi-storey frame structures including soil-structure interaction has been developed using the Spectral Element Method (SEM). The structure was modeled using the SEM, while the dynamic stiffness matrix of the soil – foundation interface was determined using the Integral Transform Method (ITM). The structure consists of one – dimensional elements: beams and columns, and two – dimensional elements: plates. The soil consists of horizontal layers over the bedrock or half space. The Projection method was used to develop the dynamic stiffness matrices for the transverse and in-plane vibration of plates with arbitrary boundary conditions. The method for coupling one-dimensional and twodimensional spectral elements was developed, as well as the coupling of the spectral elements with the soil spring – dashpot elements. Using the proposed numerical model 3D frame structures founded on soil of variable stiffness and subjected to ground vibrations induced by traffic were analyzed.", "У овом раду приказан је 3Д нумерички модел за динамичку анализу вишеспратних рамовских конструкција узимајући у обзир садејство између тла и конструкције. За моделирање конструкције коришћен je Метод спектралних елемената, док је динамичка матрица крутости тла одређена применом Методе интегралних трансформација. Конструкција се састоји од једнодимензионалних елемената: греда и стубова, и дводимензионалних елемената: плоча. Тло се састоји од хоризонталних слојева изнад круте базе или полупростора. Матрице крутости плоча напрегнутих на савијање и у својој равни одређене су применом Методе пројекције. Развијен је поступак спрезања једнодимензионалних и дводимензионалних спектралних елемената, као и спрезање тако формираног модела са тлом. Примена формираног 3Д модела приказана је на анализи 3Д рамовских конструкција фундираних на тлу различите крутости, изложених дејству вибрација изазваних саобраћајним оптерећењем." ]
[ "" ]
application/pdf
sr
Универзитет у Београду, Грађевински факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "dynamics of structures", "spectral element method", "integral transform method", "soil-structure interaction", "substructure approach", "frequency domain", "dynamic stiffness" ]
[ "динамика конструкција", "метод спектралних елемената", "метод интегралних трансформација", "интеракција конструкција-тло", "метода подструктура", "фреквентни домен", "динамичка крутост" ]
[ "Dynamic analysis of soil-structure system using spectral element method", "Динамичка анализа система тло-конструкција применом спектралних елемената." ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Нефовска-Даниловић, Марија Т.; Dinamička analiza sistema tlo-konstrukcija primenom spektralnih elemenata.;
[ "https://nardus.mpn.gov.rs/bitstream/id/3977/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/3977/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2188" ]
10.2298/bg20130514nefovskadanilovic
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/2086
123456789-2086.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:8220/bdef:Content/download
no
Функционално-морфолошке и етолошке карактеристике шумске корњаче (Testudo hermanni)
Characteristics of functional morphology and ethology of Hermann's tortoises (Testudo hermanni)
[ "Tomović, Ljiljana", "Bonnet, Xavier" ]
[ "Ivanović, Ana", "Pavković-Lučić, Sofija", "Đorđević, Sonja" ]
Golubović, Ana V.
2016-01-05T11:46:43
[ "2016-01-05T11:46:43", "2020-07-03T08:09:23" ]
2014-03-01
[ "https://nardus.mpn.gov.rs/handle/123456789/2086", "http://eteze.bg.ac.rs/application/showtheses?thesesId=1254", "https://fedorabg.bg.ac.rs/fedora/get/o:8220/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=45441039" ]
[ "Специфичан план грађе (чврст коштани оклоп и кратки екстремитети) копненим корњачама поставља знатна ограничења у кретању и превазилажењу препрека у станишту. Стога „компензација понашањем“ игра веома значајну улогу у кретању копнених корњача. Да би се тестирале унутар- и међупопулационе разлике у агилности и понашању шумске корњаче (Testudo hermanni), вршена су три експеримента. У првом експерименту корњаче су постављане на леђа и праћено је понашање током превртања у усправан положај. Током другог експеримента, теста скакања, корњаче су постављане на „негативну висинску препреку“ (хоризонталну површину на извесној висини изнад подлоге) и праћено је понашање током силажења са ње. Трећим експериментом је тестирано понашање корњача када се нађу „заглављене“ у вегетацији. Овом приликом је на предњи део пластрона закачена омча ужета на чијем другом крају се налазио динамометар. Праћено је понашање за време ослобађања од експерименталне апаратуре и мерена је мишићна снага корњача. Параметри понашања добијени овим тестовима су анализирани у односу на: морфолошке одлике (дужинске мере оклопа и ногу, као и облик и величину карапакса), пол, узрасне категорије јединки и одлике станишта. Показано је да величина и облик тела значајно утичу на превртање корњача. Крупније корњаче се теже преврћу него ситније, при чему висина оклопа има позитиван, а дужина коштаних мостова негативан ефекат на успешност усправљања. Међутим, применом геометријске морфометрије показано је да промене облика оклопа услед повећања величине адултних корњача, међу којима је и висина оклопа, ипак нису позитивно асоциране са превртањем..." ]
[ "Unique body plan of terrestrial chelonians (rigid shell and short limbs) imposes significant limitations to movements of these animals. Thus “behavioural compensation” plays an important role in overcoming obstacles in their habitat, on the daily basis. To test intra- and inter-population differences in agility and behaviour of Hermann’s tortoises (Testudo hermanni) during overcoming obstacles in their habitat, three experiments were performed. In the first experiment tortoises were positioned on their backs and their behaviour was noted during overturning. For the second experiment, “jumping” test, tortoises were placed on „negative height obstacle“ (i.e. horizontal surface above substrate) and their behaviour was noted during getting off it. The third experiment tested behaviour of tortoises when they are “stuck” in dense vegetation. For this test, a non-stretchable rope was attached on the front, protuberant part of the plastron, while the dynamometer was attached on the other end of the rope. Behaviour was noted during the animals’ attempts to liberate from the experimental apparatus, and their muscular strength was measured. Behavioural parameters gathered during these tests were analysed with regard to: morphological traits (length measurements of shell and legs, and carapace shape and size), sex, age categories and habitat features. Body size and shape had significant effects on righting behaviour. Larger tortoises were less efficient in overturning than the smaller ones. Shell height showed positive, while length of bony bridges showed negative effect on the overturning efficiency. However, geometric morphometrics showed that allometric shape changes in adult tortoises (e.g. increase in relative carapace height) are not positively associated with overturning. Differences between sexes in behaviour and agility were significant only for the righting test, in which males were more successful, in half of the tested localities. Males were previously considered more agile than females, but these experiments propose that effect of sex on agility of tortoises is not as significant. Effect of age classes is analysed only in the “jumping” test..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "копнене корњаче", "Testudo hermanni", "функционална морфологија", "етологија", "агилност" ]
[ "tortoises", "Testudo hermanni", "functional morphology", "ethology", "agility" ]
[ "Функционално-морфолошке и етолошке карактеристике шумске корњаче (Testudo hermanni)", "Characteristics of functional morphology and ethology of Hermann's tortoises (Testudo hermanni)" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Томовић, Љиљана; Боннет, Xавиер; Павковић-Лучић, Софија; Ђорђевић, Соња; Ивановић, Aна; Голубовић, Aна В.; Funkcionalno-morfološke i etološke karakteristike šumske kornjače (Testudo hermanni);
[ "https://nardus.mpn.gov.rs/bitstream/id/2078/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2078/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2086" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/11742
123456789-11742.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:20740/bdef:Content/download
no
Varijante promotora gena za uridin-difosfat-glukuronoziltransferazu 1A1 kao modulatori biohemijskog fenotipa i populaciono farmakogenetički markeri
Promoter variants of the gene for uridine-diphosphate-glucuronosyltransferase 1A1 as modulators of biochemical phenotype and population pharmacogenetic markers.
[ "Zukić, Branka" ]
[ "Pavlović, Sonja", "Radović, Svetlana", "Zukić, Branka", "Pavlović, Sonja" ]
Vuković, Marija
2020-01-27T12:20:34
[ "2020-01-27T12:20:34", "2020-07-03T08:07:58" ]
2019-09-24
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=7091", "https://nardus.mpn.gov.rs/handle/123456789/11742", "https://fedorabg.bg.ac.rs/fedora/get/o:20740/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51730447" ]
[ "amil ija en zi ma u ridin -dif osfat -glu kuronoziltransferaza (U GT ) kat a lizuj e glukuronidaciju širokog spektra endobiotika i ksenobiotika. UGT1 familija je kodirana genskim kompleksom UGT1, koji se sastoji od 13 proksimalnih varijabilnih egzona i od 4 zajednička distalna egzona. Alternativnom obradom nastaje više transkripata, među kojima je posebno značajan onaj čiji je produkt UGT1A1 enzim, koji učestvuje u metabolizmu bilirubina i mnogih lekova. Više od 60 varijanti je detektovano u UGT1A1 genu, koje su povezane sa patološkim stanjima. Varijante u TATA boksu promotora UGT1A gena se razlikuje po broju TA ponovaka. Wild type UGT1A1*1 sadrži 6 TA ponovaka (TA6), dok su varijante koje sadrže 5, 7 ili 8 TA ponovaka označene kao UGT1A1*36 (TA5), UGT1A1*28 (TA7) i UGT1A1*37 (TA8). Transkripciona aktivnost promotora je manja što je veći broj ponovaka u odnosu na TA6. Žilberov sindrom (ŽS) je često kliničko stanje koje karakteriše hiperbilirubinemija i ikterus. Uzrokovan je smanjenom aktivnošću UGT1A1 enzima. Najčešće detektovana varijanta koja se nalazi u osnovi ŽS je UGT1A1*28. Njena učestalost varira u različitim populacijama, a kod belaca je prisutna sa 26% do 31%. ŽS je blago oboljenje, ali kao komorbiditet može dovesti do pogrešne dijagnoze bolesti jetre ili hemolitičkih procesa. U tim slučajevima je neophodno potvrditi ili isključiti dijagnozu ŽS molekularnom analizom UGT1A1 gena. Malo se zna o uticaju varijanti promotora UGT1A1 gena na scenario kod oboljenja jetre. Samo u nekoliko slučajeva je kod pacijenata sa hepatitisom C sprovedeno genetičko testiranje na UGT1A1*28, i pošto je dokazana pozitivnost objasnila detektovan nivo bilirubina, pacijenti su lečeni po standardnom protokolu i postigli su kontinuiran virusološki odgovor. Kod hemolitičkih anemija, kao što je talasemija, takođe je pokazano da je nivo bilirubina povezan sa pojavom komplikacija. Stoga se varijante u UGT1A1 genu tretiraju kao tercijarni modifikatori kod beta- talasemijskih sindroma..." ]
[ "The uridine-diphosphate-glucuronosyltransferase enzyme family (UGT) catalyse the glucuronidation of a wide range of endobiotics and xenobiotics. The UGT1 family is encoded by the UGT1 gene complex consisting of 13 proximal variable exons and 4 common distal exons. Alternative splicing gives multiple transcripts, among which the UGT1A1 enzyme product, which is involved in the metabolism of bilirubin and many drugs, is particularly important. More than 60 variantshave been detected in the UGT1A1 gene, which are associated with pathological conditions. Variants in the TATA box of the UGT1A gene promoter differ in the number of TA repeats. Wild type UGT1A1 contains 6 TA repeats (TA6), while variants with 5, 7 or 8 TA repeats are designated UGT 1A1*36 (TA5), UGT1A1*28 (TA7) and UGT1A1*37 (TA8). The transcription activity of the promoter decreased accordingly to higher number of repeats compared to TA6. Gilbert's syndrome (GS) is often a clinical condition characterized by hyperbilirubinaemia and icterus. It is caused by a reduced activity of UGT1A1 enzymes. The most commonly detected variant found in the basis of GS is UGT1A1*28. Its frequency varies in different populations, and whites are present from 26% to 31%. GS is a mild illness, but as a comorbidity it can lead to a wrong diagnosis of liver disease or hemolytic processes. In these cases, it is necessary to confirm or exclude the diagnosis of GS by molecular analysis of the UGT1A1 gene. The effect of variants of UGT1A1 gene on the development in liver disease is poorly known. In only a few cases, genetic testing for UGT1A1*28 was performed in patients with hepatitis C, and since the confirmed positivity explained the detected level of bilirubin, patients were treated by standard protocol and achieved a continious viral response. In hemolytic anemia, such as thalassemia, it has also been shown that the level of bilirubin is associated with the numerous of complications. Therefore, the TA promoter variants in the UGT1A1 gene are treated as tertiary modifiers in beta-talasemia syndromes..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "UGT1A1", "Žilberov sindrom", "hiperbilirubinemija", "farmakogenetički marker", "populaciona studija", "personalizovana medicina" ]
[ "UGT1A1", "Gilbert’s syndrome", "hyperbilirubinemia", "pharmacogenetic marker", "population study", "personalized medicine" ]
[ "Varijante promotora gena za uridin-difosfat-glukuronoziltransferazu 1A1 kao modulatori biohemijskog fenotipa i populaciono farmakogenetički markeri" ]
[ "Promoter variants of the gene for uridine-diphosphate-glucuronosyltransferase 1A1 as modulators of biochemical phenotype and population pharmacogenetic markers." ]
doctoralThesis
[ "BY-NC-ND" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1753/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1754/IzvestajKomisije21600.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1753/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1754/IzvestajKomisije21600.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_11742" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/2018
123456789-2018.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:3435/bdef:Content/download
no
Uloga hormona jajnika u involuciji timusa pacova
Role of ovarian hormones in thymic involution in rats
[ "Leposavić, Gordana" ]
[ "Leposavić, Gordana", "Božić, Biljana", "Božić, Biljana", "Vučević, Dragana" ]
Perišić, Milica M.
2016-01-05T11:45:18
[ "2016-01-05T11:45:18", "2020-07-03T08:07:23" ]
2012-09-28
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=68", "https://nardus.mpn.gov.rs/handle/123456789/2018", "https://fedorabg.bg.ac.rs/fedora/get/o:3435/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=41905423" ]
[ "Timus je primarni limfoidni organ koji obezbeđuje mikrosredinu neophodnu za diferencijaciju/sazrevanje T-limfocita. Tokom ontogeneze, ovaj organ trpi značajne strukturne i funkcionalne promene koje se zbirno označavaju kao involucija timusa, i manifestuju se kao smanjena efikasnosti timopoeze i posledično, smanjen izlazak novoformiranih T-limfocita na periferiju. Involucija timusa je kod glodara najizraženija u periodu sticanja polne zrelosti. Veliki broj literaturnih podataka ukazuje na to da hormoni hipotalamo-hipofizo-gonadne (H-H-G) osovine, pre svega hormoni gonada, utiču na razvoj i involuciju timusa. Međutim, uprkos brojnim istraživanjima u proteklim decenijama, uloga hormona jajnika u inicijaciji i održavanju/progresiji involucije timusa nije do kraja rasvetljena. Cilj ove doktorske disertacije je bio da se ispita uloga hormona jajnika u inicijaciji i održavanju/progresiji involucije timusa. U tom cilju ispitivan je uticaj jednokratnog davanja testosterona u kritičnom neonatalnom uzrastu, koji dovodi do odlaganja sazrevanja H-H-G osovine i promena u obrascu sekrecije hormona jajnika kod adultnih životinja (neonatalna androgenizacija) i uklanjanja jajnika ženkama pacova u uzrastu od 10 meseci, kada su involutivne promene timusa jasno izražene, na građu timusa i timopoezu u ranom adultnom uzrastu; odnosno u uzrastu od 11 meseci. Posebno je ispitivan, mogući, indirektan uticaj promena u sekreciji hormona jajnika na aktivnost timusnog kateholaminergičkog regulatornog sistema. Neonatalna androgenizacija, delujući na različite stadijume diferencijacije/sazrevanja T-ćelija, od ulaska/diferencijacije progenitorskih ćelija do linijskog usmeravanja timocita, povećala je efikasnost timopoeze (na osnovu većeg broja svežih timusnih emigranata u perifernoj krvi i slezini), uključujući, najverovatnije, i povećano stvaranje ćelija regulatornog fenotipa (CD4+CD25+FoxP3+ i CD161+TCRαβ+ ćelije). Pored toga, kod ovih životinja je uočeno favorizovano stvaranje ćelija CD4-CD8+TCRαβ++ fenotipa (fenotipska defeminizacija/maskulinizacija procesa timopoeze). Uprkos povećanoj efikasnosti timopoeze, nepromenjena relativna zastupljenost timusnih epitelnih ćelija (TEC) kao i nivo iRNK za IL-6, a smanjen nivo iRNK za IL-7 u tkivu timusa, ukazuju, da je kod neonatalno androgenizovanih životinja, u uzrastu od 3 meseca, najverovatnije, došlo do pokretanja mehanizama negativne povratne sprege, koji ograničavaju ekspanziju TEC i timopoezu. Ovarijektomija u uzrastu od 10 meseci, nakon 30. dana, imala je za posledicu povećanje relativne zastupljenosti TEC i efikasnosti timopoeze (uključujući i povećano stvaranje ćelija regulatornog fenotipa), što se manifestovalo povećanim brojem svežih timusnih emigranata CD4+ i CD8+ fenotipa u perifernoj krvi i slezini. Međutim, uprkos regeneraciji epitelne komponente timusa i povećanoj efikasnosti timopoeze, smanjen nivo iRNK za IL-6 i IL-7 u tkivu timusa ovarijektomisani životinja sugeriše da je kod ovih životinja došlo do aktivacije intratimusnih mehanizama negativne povratne sprege koji ograničava ekspanziju TEC koje sintetišu IL-6 i efikasnost timopoeze. U zaključku, nalazi dobijeni u ovoj disertaciji ukazuju da neonatalna androgenizacija menja kinetiku postnatalnog razvoja timusa i polno specifičan obrazac diferencijacije/sazrevanja timocita i odlaže, ali vrlo verovatno ne sprečava involuciju timusa, dok uklanjanje hormona jajnika u uzrastu kada su involutivne promene timusa uznapredovale dovodi do delimične reverzije ovih promena i efikasnije timopoeze, koji su, najverovatnije, vremenski ograničenog trajanja. Pored toga, pokazano je da se uticaj hormona jajnika na ove procese odvija i indirektno, menjanjem efikasnosti modulatornog delovanja noradrenalina na timopoezu." ]
[ "The thymus is a primary lymphoid organ that provides the microenvironment necessary for the differentiation/maturation of T-lymphocytes. During ontogeny, thymus undergoes significant structural and functional changes leading to reduced efficiency of thymopoiesis and, consequently, reduced output of newly generated Tlymphocytes. These changes are collectively referred to as thymic involution. The most profound thymic changes in rodents, occur around puberty. There is an accumulating body of evidence indicating that hormones of the hypothalamic-pituitary-gonadal (H-PG) axis, particularly gonadal hormones, influence thymic development and involution. However, despite extensive research in the past decades, the role of ovarian hormones in the initiation and maintenance/progression of thymic involution is not fully understood. Bearing all aforementioned in mind, this dissertation was aimed to elucidate the role of ovarian hormones in initiation and maintenance/progression of thymic involution. To this end the effects of single injection of testosterone in critical postnatal period, which postpones H-P-G axis maturation and affects the gender-specific pattern of gonadal hormone secretion in adult animals (neonatal androgenization), and ovarian hormone removal in rats with advanced thymic involutive changes (10-month-old), on thymic structure and function, were examined in 3-month-old and 11-month-old female rats, respectively. In addition, the putative indirect, catecholamine-mediated effects of the neonatal androgenization and ovariectomy on thymopoiesis were explored. Neonatal androgenization, affecting distinct stages of thymocyte differentiation/maturation (from progenitor cell entry and differentiation to thymocyte lineage commitment), increased the efficiency of thymopoiesis and consequently thymic output (as evidenced by the increased number of recent thymic emigrants in peripheral blood and spleen), including, most likely, increased output of the cells with regulatory phenotype (CD4+CD25+FoxP3+ and CD161+TCRαβ+ cells). In addition, neonatal treatment with testosterone skewed thymocyte lineage commitment towards CD4-CD8+TCRαβ++ cells (phenotypic defeminization/masculinization of thymopoiesis). Despite the increased efficiency of thymopoiesis, unaltered relative abundance of thymic epithelial cells (TEC) and the thymic level of IL-6 mRNA, and reduced thymic level of IL-7 mRNA, indicated, most likely, activation of intrathymic negative feedback mechanisms limiting TEC proliferation and thymopoiesis in 3- month-old neonatally androgenized rats. Ovariectomy at the age of 10 months led to the expansion of TECs and greater efficiency of thymopoiesis (including generation of cells with regulatory phenotype), increasing the number of both CD4+ and CD8+ recent thymic emigrants in peripheral blood and spleen of 11-month-old rats, compared with age-matched controls. However, despite the regeneration of the thymic epithelial component and increased efficiency of thymopoiesis, reduced thymic levels of mRNA for IL-6 and IL-7 in ovariectomized rats indicated activation of intrathymic negative feedback mechanisms limiting the expansion of IL-6-synthesizing TECs and thymopoietic efficiency in these animals. In conclusion, the data obtained in this dissertation indicate that neonatal androgenization alters the kinetics of postnatal thymic development and sex-specific pattern of thymocyte differentiation/maturation and postpones thymic involution, but, most likely, does not prevent it completely. The removal of ovarian hormones in female rats with advanced thymic involutive changes leads to partial reversion of these changes, and an increase in thymopoietic efficiency of, most likely, limited duration. In addition, these data indicate that ovarian hormones might affect thymic development/involution not only directly, but also indirectly by diminishing the efficiency of noradrenaline-mediated modulation of thymopoiesis." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175050/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "involucija timusa", "hormoni jajnika", "timusne epitelne ćelije", "timopoeza", "IL-6", "IL-7", "noradrenalin", "adrenergički receptori", "sveži timusni emigranti", "regulatorne T ćelije" ]
[ "thymic involution", "ovarian hormones", "thymic epithelial cells", "thymopoiesis", "IL-6", "IL-7", "noradrenaline", "adrenergic receptors", "recent thymic emigrants", "regulatory T-cells" ]
[ "Uloga hormona jajnika u involuciji timusa pacova", "Role of ovarian hormones in thymic involution in rats" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Лепосавић, Гордана; Вучевић, Драгана; Божић, Биљана; Божић, Биљана; Лепосавић, Гордана,; Перишић, Милица М.; Улога хормона јајника у инволуцији тимуса пацова; Улога хормона јајника у инволуцији тимуса пацова;
[ "https://nardus.mpn.gov.rs/bitstream/id/1581/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1581/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2018" ]
10.2298/bg20120928perisic
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/4171
123456789-4171.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:10223/bdef:Content/download
no
Molekularni markeri hronične inflamacije i apoptoze kod inflamatornih bolesti creva
Molecular markers of chronic inflammation and apoptosis in inflammatory bowel diseases
[ "Nikčević, Gordana" ]
[ "Pavlović, Sonja", "Brajušković, Goran", "Nikčević, Gordana", "Pavlović, Sonja" ]
Stanković, Biljana B.
2020-07-03T08:07:29
[ "2020-07-03T08:07:29" ]
2015-07-06
[ "https://nardus.mpn.gov.rs/handle/123456789/4171", "http://eteze.bg.ac.rs/application/showtheses?thesesId=2312", "https://fedorabg.bg.ac.rs/fedora/get/o:10223/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024962482" ]
[ "Hronične inflamatorne bolesti creva (HIBC) predstavljaju rastući globalni zdravstveni problem, posebno u belim populacijama, uključujući i srpsku populaciju. Ova studija obuhvatila je dva tipa HIBC - inflamatornu bolest creva (IBC), koju čine Kronova bolest (KB) i ulcerozni kolitis (UK); i celijačnu bolest (CB). HIBC su kompleksne bolesti u čijoj, još uvek potpuno nerazjašnjenoj etiologiji učestvuju genetički, sredinski i imunološki faktori. Razvoj IBC je rezultat poremećenog imunološkog odgovora na crevnu mikrofloru kod genetički podložnih osoba. Ustanovljen je veliki broj asocijacija između IBC i varijanti u genima čiji proteinski produkti učestvuju u nespecifičnom imunskom odgovoru. Oštećenje intestinalne mukoze kod pacijenata sa IBC dešava se kao posledica deregulacije inflamatornih i apoptotskih procesa koji utiču na dugovečnost T ćelija i integritet epitelijalne barijere. Protein NF-κB je jedan od ključnih regulatora transkripcije gena eksprimiranih tokom ovih patogenetskih promena. U mukozi pacijenata sa KB dolazi do formiranja granuloma, limfoidnih mikrostruktura karakterističnih i za druge inflamatorne bolesti, među kojima je reumatoidni artritis (RA). Zbog toga su KB i RA klasifikovane kao granulomatozne bolesti. U poređenju sa brojnim, još uvek nedovoljno definisanim genetičkim faktorima rizika za razvoj IBC, nasledna komponenta u razvoju CB je dobro okarakterisana i najviše je povezana sa specifičnim varijantama gena HLA-DQ. Jedan od ciljeva ove studije je bio da ispita asocijaciju između HIBC i određenih genetičkih varijanti. Tačnije, analizirana je povezanost IBC sa varijantama u genima NOD2, TLR4, TNF-α, IL-6, IL-1β i IL-1RN, i ispitan je njihov prediktivni značaj za pojavu IBC. Varijanta u genu IL-6 ispitana je kao faktor rizika za nastanak dve granulomatozne bolesti, KB i RA. Takođe, analizirana je distribucija genotipova HLA-DQ u grupi pedijatrijskih pacijenata obolelih od CB i procenjen je rizik za pojavu CB koju ovi genotipovi nose. Sledeći cilj je bila analiza nivoa transkripcije proinflamatornih gena IL-6 i TNF-α, i apoptotskih gena Bcl-2, Bax, Fas i FasL u intestinalnoj mukozi i perifernoj krvi pacijenata obolelih od KB, kako bi se definisali ekspresioni profili gena uključenih u patogenezu KB i ispitao njihov dijagnostički potencijal. Poslednji cilj u okviru istaživanja vezanog za IBC odnosio se na analizu DNK vezujuće aktivnosti NF-κB iz jedarnih ekstrakata intestinalne mukoze pacijenata obolelih od KB..." ]
[ "Chronic inflammatory bowel diseases (CIBDs) remain an expanding global health problem, particularly in most Caucasian populations, including Serbian. The focus of this study included two types of chronic inflammatory bowel diseases - inflammatory bowel disease (IBD), which comprises Crohn’s disease (CrD) and ulcerative colitis (UC); and celiac disease (CD). These are complex diseases with various genetic, environmental and immunological risk factors, whose etiologies are not yet fully resolved. The development of IBD is a result of abnormal immune response that occurs in genetically susceptible individuals against the enteric flora. Many studies have investigated associations between IBD occurrence and variants in genes that encode proteins involved in nonspecific immune response. Intestinal mucosal damage in CrD patients occurs as a result of the deregulation of inflammatory and apoptotic processes, which influence T cell longevity and epithelial barrier integrity. Regulatory protein NF-κB has a key role in the transcription of genes whose products are involved in these pathogenic events. Another property of CrD is formation of granulomas, which can also occur in other diseases, such as rheumatoid arthritis (RA). For that reason, CrD and RA are categorized as granulomatous diseases. Compared with numerous, still insufficiently defined genetic risk factors for the development of IBD, a genetic component in CD development is well characterized and mostly associated with specific variants of HLA-DQ genes. One of the aims of this study was to evaluate disease-gene associations. More precisely, we examined IBD associations with several genetic variants - variants in NOD2, TLR4, TNF-α, IL-6, IL-1β and IL-1RN genes, and investigated their contribution in the prediction of IBD occurrence. Additionally, we analyzed variant in IL-6 gene as a risk factor for two types of granulomatous diseases, CrD and RA. Also, we inspected the distribution of HLA-DQ genotypes in the group of pediatric celiac patients and estimated the risk of CD development that these genotypes confer. Another aim was the analysis of mRNA level of proinflammatory IL-6 and TNF-α, and apoptotic Bcl-2, Bax, Fas and FasL genes in intestinal mucosa, as well as in the peripheral blood of CrD patients, in order to reveal the expression patterns involved in the pathogenesis of CrD..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41004/RS//", "info:eu-repo/grantAgreement/MESTD/MPN2006-2010/143051/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "hronične inflamatorne bolesti creva", "Kronova bolest", "ulcerozni kolitis", "celijačna bolest", "granulomatozne bolesti", "proinflamatorni geni", "apoptotski geni", "aleli HLADQ", "prediktivni modeli", "NF-κB." ]
[ "chronic inflammatory bowel diseases", "Crohn’s disease", "ulcerative colitis", "celiac disease", "granulomatous diseases", "proinflammatory genes", "apoptotic genes", "HLA-DQ alleles", "predictive modeling", "NF-κB" ]
[ "Molekularni markeri hronične inflamacije i apoptoze kod inflamatornih bolesti creva", "Molecular markers of chronic inflammation and apoptosis in inflammatory bowel diseases" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Никчевић, Гордана; Павловић, Соња; Брајушковић, Горан; Никчевић, Гордана; Павловић, Соња; Станковић, Биљана Б.; Молекуларни маркери хроничне инфламације и апоптозе код инфламаторних болести црева; Молекуларни маркери хроничне инфламације и апоптозе код инфламаторних болести црева;
[ "https://nardus.mpn.gov.rs/bitstream/id/1610/Disertacija76.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1611/Biljana_Stankovic_Referat_BF.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1610/Disertacija76.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1611/Biljana_Stankovic_Referat_BF.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_4171" ]
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https://nardus.mpn.gov.rs/handle/123456789/10051
123456789-10051.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:18502/bdef:Content/download
no
Molekularni mehanizmi antiproliferativnog dejstva rutenijum(II)-arenskog kompleksa sa izohinolin-3-karboksilnom kiselinom kao ligandom u uslovima in vitro
Molecular mechanisms of the antiproliferative activity of ruthenium(II)-arene complex with isoquinoline-3-carboxylic acid as ligand under in vitro conditions
[ "Gligorijević, Nevenka" ]
[ "Jelić, Mihajlo", "Grgurić Šipka, Sanja" ]
Jovanović, Katarina K.
2018-11-07T15:53:12
[ "2018-11-07T15:53:12", "2020-07-03T08:07:00" ]
2016-09-22
[ "https://nardus.mpn.gov.rs/handle/123456789/10051", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6093", "https://fedorabg.bg.ac.rs/fedora/get/o:18502/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025140402" ]
[ "Još od otkrića cisplatine sintetisan je veliki broj kompleksa prelaznih metala, čija je antikancerska aktivnost ispitana. Naročitu pažnju zaslužuju jedinjenja zasnovana na rutenijumu kao metalu koja su pokazala antitumorsku i antimetastatsku aktivnost, sa potencijalnom primenom kod tumora rezistentnih na cisplatinu, ili kao alternative cisplatini. Cilj ove disertacije je ispitivanje molekularnih mehanizama antiproliferativne aktivnosti rutenijum(II)-arenskog kompleksa sa izohinolin-3-karboksilnom kiselinom kao ligandom. Rezultati ispitivanja citotoksičnog potencijala sedam novih jedinjenja opšte formule [Ru(η6-p-cimen)(L1-7)Cl] su pokazali da je kompleks sa L = izohinolin-3-karboksilnom kiselinom (RuT7) u odnosu na ostale komplekse iz serije, ispoljio izraženu citotoksičnu aktivnost, sa IC50 vrednostima u opsegu od 18,5 do 84,2 μM. Ovaj kompleks je pokazao dva puta veću aktivnost na HeLa ćelijama nego na normalnoj ćelijskoj liniji MRC-5, što potvrđuju i IC50 vrednosti određene nakon inkubacionog perioda od 48 h (45,4 ± 3,0 i 84,2 ± 5,7 μM). Analiza ćelijskog ciklusa HeLa ćelija koje su tretirane RuT7 kompleksom pokazala je zaustavljanje ćelija u S fazi ćelijskog ciklusa, kao i povećanje broja ćelija u sub-G1 populaciji. Aneksin V-FITC/PI esej, kao i morfološka analiza ćelija na fluorescentnom mikroskopu, pokazali su da ispitivano jedinjenje ima potencijal indukcije apoptoze. Ispitivanje akumulacije rutenijuma unutar ćelija je pokazalo prisustvo rutenijuma u ćelijskoj unutrašnjosti nakon 6 h inkubacije (8,9 ng Ru/106 ćelija). U cilju detaljnog ispitivanja mehanizma dejstva RuT7 kompleksa na HeLa ćelijama, urađena je mikroerej analiza promena u genskoj ekspresiji na celom transkriptomu HeLa ćelija. Analiza funkcionalnih kategorija i signalnih i biohemijskih puteva koji se menjaju tokom odgovora HeLa ćelija na tretman RuT7 kompleksom je pokazala da se pod uticajem tretmana aktiviraju molekularni mehanizmi koji vode ćelije u apoptozu i to preko unutrašnjeg (mitohondrijskog) signalnog puta. Do aktiviranja mitohondrijskog apoptotskog puta dolazi usled direktnog vezivanja RuT7 kompleksa za DNK molekul, kao i indirektnim oštećenjem DNK usled dejstva reaktivnih kiseoničnih vrsta. Statistička analiza promena u ekspresiji genskih setova uključenih u poznate puteve toksičnosti koje su izazvane lekovima je pokazala manju predviđenu toksičnost RuT7 kompleksa u poređenju sa cisplatinom. Sveobuhvatno, dobijeni rezultati predstavljaju osnovu za dalja istraživanja RuT7 kompleksa u okviru studija na životinjama i prekliničkim ispitivanjima kao potencijalnog kandidata za hemioterapeutika." ]
[ "Since the discovery of cisplatinum, many transition metal complexes have been synthesized and assayed for antineoplastic activity. In recent years, ruthenium-based compounds have emerged as promising antitumor and antimetastatic agents with potential uses in platinum-resistant tumors or as alternatives to platinum. The aim of this thesis was to investigate molecular mechanisms of the antiproliferative activity of ruthenium(II)-arene complex with isoquinoline-3-carboxylic acid as ligand. The results of investigation of the cytotoxic potential of seven new compounds of the general formula [Ru(η6-p-cymene)(L1-7)Cl] showed that the complex with L = isoquinoline-3-carboxylic acid (RuT7) was two times as active on HeLa cells compared to normal cell line MRC-5, as indicated by IC50 values determined after 48 h of incubation (45,4 ± 3,0 vs. 84,2 ± 5,7 μM, respectively). Cell cycle analysis of HeLa cells treated with RuT7 showed S phase arrest and an increase in sub-G1 population. The apoptotic potential of the title compound was confirmed with the Annexin V-FITC/PI assay together with a morphological evaluation of cells using fluorescent microscopy. Analysis of the intracellular accumulation of ruthenium showed 8,9 ng Ru/106 cells after 6 h of incubation. To gain further insight in the molecular mechanism of action of RuT7 on HeLa cells, a whole-transcriptome microarray gene expression analysis was performed. Analysis of functional categories and signaling and biochemical pathways associated with the response of HeLa cells to treatment with RuT7 showed that it leads the cells through the intrinsic (mitochondrial) apoptotic pathway, via indirect DNA damage due to the action of reactive oxygen species, and through direct DNA binding of RuT7. Statistical analysis for enrichment of gene sets associated with known drug-induced toxicities identified fewer associated toxicity profiles in RuT7-treated cells compared to cisplatin treatment. Altogether these results provide the basis for further development of RuT7 in animal and pre-clinical studies as a potential drug candidate." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41026/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "rutenijum(II)-arenski kompleksi", "citotoksičnost", "maligne ćelije", "ćelijski ciklus", "apoptoza", "genska ekspresija" ]
[ "rutenium(II)-arene complexes", "cytotoxicity", "malignant cells", "cell cycle", "apoptosis", "gene expression" ]
[ "Molekularni mehanizmi antiproliferativnog dejstva rutenijum(II)-arenskog kompleksa sa izohinolin-3-karboksilnom kiselinom kao ligandom u uslovima in vitro" ]
[ "Molecular mechanisms of the antiproliferative activity of ruthenium(II)-arene complex with isoquinoline-3-carboxylic acid as ligand under in vitro conditions" ]
doctoralThesis
[ "BY-NC-ND" ]
Глигоријевић, Невенка; Гргурић Шипка, Сања; Јелић, Михајло; Јовановић, Катарина К.; Молекуларни механизми антипролиферативног дејства рутенијум(ИИ)-аренског комплекса са изохинолин-3-карбоксилном киселином као лигандом у условима ин витро; Молекуларни механизми антипролиферативног дејства рутенијум(ИИ)-аренског комплекса са изохинолин-3-карбоксилном киселином као лигандом у условима ин витро;
[ "https://nardus.mpn.gov.rs/bitstream/id/1503/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1504/IzvestajKomisije17865.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1503/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1504/IzvestajKomisije17865.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10051" ]
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https://nardus.mpn.gov.rs/handle/123456789/7885
123456789-7885.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:15113/bdef:Content/download
no
Fiziološki i molekularno-genetički pokazatelji međuvrsne hibridizacije u okviru roda Centaurium Hill (Gentianaceae)
Physiological and molecular genetic indicators of interspecific hybridization within the genus Centaurum Hill (Gentianaceae)
[ "Šiler, Branislav" ]
[ "Jelić, Mihailo", "Mišić, Danijela", "Sabovljević, Aneta", "Skorić, Marijana" ]
Banjanac, Tijana B.
2017-04-17T14:58:59
[ "2017-04-17T14:58:59", "2020-07-03T08:06:35" ]
2016-12-27
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=4809", "https://nardus.mpn.gov.rs/handle/123456789/7885", "https://fedorabg.bg.ac.rs/fedora/get/o:15113/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025151154" ]
[ "Vrste roda Centaurium Hill često hibridizuju u prirodi. Brojni primeri ukazuju na značaj poliploidizacije i hibridizacije tokom specijacije u okviru ovog roda. Ukrštanjem Centaurium erythraea Rafn × C. littorale (Turner) Gilmour u uslovima in vitro, dobijeno je alotetraploidno potomstvo, što ide u prilog hipotezi postojanja nedovoljno efikasnih prereproduktivnih barijera, koje bi sprečavale hibridizaciju. Primenjene metode za detekciju hibridnih jedinki u uslovima in vitro su uspešno iskorišćene i u slučaju prirodnih populacija poreklom sa Subotičko-horgoške peščare. Novi takson, nazvan Centaurium pannonicum, je heksaploid nastao iz ukrštanja C. erythraea × C. littorale ssp. compressum (Hayne) Kischner, nakon fertilizacije, tj. spajanja diploidnog i tetraploidnog jedra. De novo razvijeni EST-SSR markeri ukazuju da C. pannonicum konvergira vrsti C. erythraea, dok je prema fitohemijskim markerima hibridni takson sličniji C. littorale ssp. compressum. Razvijeni setovi EST-SSR i fitohemijskih markera, su transferabilni i na druge vrste roda Centaurium, i mogu značajno doprineti daljim istraživanjima međuvrsne hibridizacije. C. pannonicum stabilno održava svoju heksaploidnu genetičku konstitutciju u prirodnim populacijama, umnogome zahvaljujući samooplodnji i/ili drugim vidovima ukrštanja u srodstvu." ]
[ "" ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173024/RS//", "info:eu-repo/grantAgreement/EC/FP7/227448/EU//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Centaurium", "hemotaksonomski markeri", "EST-SSR", "međuvrsna hibridizacija", "polimorfizam", "poliploidija", "RAPD" ]
[ "Centaurium", "chemotaxonomic markers", "EST-SSR", "interspecific hybridization", "polymorphism", "polyploidy", "RAPD" ]
[ "Fiziološki i molekularno-genetički pokazatelji međuvrsne hibridizacije u okviru roda Centaurium Hill (Gentianaceae)" ]
[ "Physiological and molecular genetic indicators of interspecific hybridization within the genus Centaurum Hill (Gentianaceae)" ]
doctoralThesis
[ "BY-NC-ND" ]
Шилер, Бранислав; Мишић, Данијела; Скорић, Маријана; Јелић, Михаило; Сабовљевић, Aнета; Бањанац, Тијана Б.; Физиолошки и молекуларно-генетички показатељи међуврсне хибридизације у оквиру рода Центауриум Хилл (Гентианацеае); Физиолошки и молекуларно-генетички показатељи међуврсне хибридизације у оквиру рода Центауриум Хилл (Гентианацеае);
[ "http://nardus.mpn.gov.rs/bitstream/id/1408/IzvestajKomisije8285.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1407/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1408/IzvestajKomisije8285.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1407/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_7885" ]
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https://nardus.mpn.gov.rs/handle/123456789/2088
123456789-2088.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:3035/bdef:Content/download
no
Bioinformatička i glikobiohemijska analiza modularne organizacije CA125 antigena čoveka
Bioinformatic and glycobiochemical analysis of human CA125 antigen modular organization
[ "Korać, Aleksandra" ]
[ "Korać, Aleksandra", "Janković, Miroslava", "Janković, Miroslava", "Milutinović, Bojana" ]
Mitić, Ninoslav M.
2016-01-05T11:46:44
[ "2016-01-05T11:46:44", "2020-07-03T08:09:25" ]
2012-11-09
[ "https://nardus.mpn.gov.rs/handle/123456789/2088", "http://eteze.bg.ac.rs/application/showtheses?thesesId=49", "https://fedorabg.bg.ac.rs/fedora/get/o:3035/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024527282" ]
[ "CА125 antigen, poznat kao marker seroznog karcinoma ovarijuma, predstavlja ekstracelularni deo mucina 16 (MUC16), koji nastaje njegovom proteolitiĉkom degradacijom. МUC16 pripada grupi transmembranskih proteina tipa I, i eksprimiran je u velikom broju embrionalnih, ali i adultnih tkiva. Njegovu primarnu strukturu karakteriše prisustvo tri regiona: N-terminalni region i region tandemskih ponovaka, koji su smešteni ekstracelularno, i C-terminalni region, koji se sastoji od transmembranskog dela i kratkog citoplazmatskog repa. Ekstracelularni region je intenzivno glikozilovan, a u intracelularnom regionu se nalazi potencijalno mesto fosforilacije. Fosforilacija predstavlja signal za proteolizu i oslobaĊanje ekstracelularnog dela molekula (СА125 antigen), koji se kao takav može detektovati u razliĉitim telesnim teĉnostima. Biološka uloga MUC16/CA125, još uvek nije razjašnjena. Dosadašnja ispitivanja funkcije MUC16 (membranska forma) su, uglavnom, bila bazirana na in vitro model sistemu ćelija ovarijalnog karcinoma, dok su literaturni podaci o aktivnosti CA125 antigena (solubilna forma) veoma retki. Solubilna forma predstavlja funkcionalni analog i kompetitor membranskoj formi i, u cirkulaciji, ona prva dospeva u kontakt sa razliĉitim tipovima normalnih ili patološki izmenjenih ćelija, ali ne postoje eksperimentalni dokazi o mogućem uticaju na njihovu adhezivnost, kao i imunomodulatorna svojstva. U ovom radu je ispitivana modularna organizacija MUC16/CA125 na bioinformatiĉkom i glikobiohemijskom nivou, sa ciljem da se bliže definišu biološki kapacitet i posebnosti prezentacije ovog molekula u kontekstu interaktoma ĉoveka. Bioinformatiĉka analiza, kao deo strategije za otkrivanje moguće biološke uloge MUC16/CA125, do sada nije korišćena u ispitivanjima ovog antigena. Pored toga, u kontekstu diskretnih bioloških funkcija, bez obzira na izuzetan biomedicinski znaĉaj, ne postoje eksperimentalni podaci o interakcijama MUC16/CA125 sa specifiĉnim klasama receptora na ćelijama krvnog sistema.CА125 antigen, poznat kao marker seroznog karcinoma ovarijuma, predstavlja ekstracelularni deo mucina 16 (MUC16), koji nastaje njegovom proteolitiĉkom degradacijom. МUC16 pripada grupi transmembranskih proteina tipa I, i eksprimiran je u velikom broju embrionalnih, ali i adultnih tkiva. Njegovu primarnu strukturu karakteriše prisustvo tri regiona: N-terminalni region i region tandemskih ponovaka, koji su smešteni ekstracelularno, i C-terminalni region, koji se sastoji od transmembranskog dela i kratkog citoplazmatskog repa. Ekstracelularni region je intenzivno glikozilovan, a u intracelularnom regionu se nalazi potencijalno mesto fosforilacije. Fosforilacija predstavlja signal za proteolizu i oslobaĊanje ekstracelularnog dela molekula (СА125 antigen), koji se kao takav može detektovati u razliĉitim telesnim teĉnostima. Biološka uloga MUC16/CA125, još uvek nije razjašnjena. Dosadašnja ispitivanja funkcije MUC16 (membranska forma) su, uglavnom, bila bazirana na in vitro model sistemu ćelija ovarijalnog karcinoma, dok su literaturni podaci o aktivnosti CA125 antigena (solubilna forma) veoma retki. Solubilna forma predstavlja funkcionalni analog i kompetitor membranskoj formi i, u cirkulaciji, ona prva dospeva u kontakt sa razliĉitim tipovima normalnih ili patološki izmenjenih ćelija, ali ne postoje eksperimentalni dokazi o mogućem uticaju na njihovu adhezivnost, kao i imunomodulatorna svojstva. U ovom radu je ispitivana modularna organizacija MUC16/CA125 na bioinformatiĉkom i glikobiohemijskom nivou, sa ciljem da se bliže definišu biološki kapacitet i posebnosti prezentacije ovog molekula u kontekstu interaktoma ĉoveka. Bioinformatiĉka analiza, kao deo strategije za otkrivanje moguće biološke uloge MUC16/CA125, do sada nije korišćena u ispitivanjima ovog antigena. Pored toga, u kontekstu diskretnih bioloških funkcija, bez obzira na izuzetan biomedicinski znaĉaj, ne postoje eksperimentalni podaci o interakcijama MUC16/CA125 sa specifiĉnim klasama receptora na ćelijama krvnog sistema.In silico analiza modularne organizacije MUC16/CA125, vršena je na osnovu podataka koji su preuzeti sa UniProtKB baze podataka, a koji se odnose na molekul izolovan iz ćelijske linije ovarijalnog karcinoma ĉoveka, OVCAR-3. Bioinformatiĉka analiza je obuhvatila: odreĊivanje homologije (korišćenjem BLAST, Basic Local Alignment Search Tool alatke); ispitivanje prisustva obrazaca, strukturnih motiva i konzerviranih domena (korišćenjem baza podataka kao što su Bgee: a dataBase for Gene Expression Evolution i Gene Ontology, CDD, ELM, BLOCKS, InterProScan, MyHits i iProClass); odreĊivanje fiziĉko-hemijskih osobina, globularnosti i odstupanja od pretpostavljene tercijarne strukture (korišćenjem ProtParam, ProtScale i GLOBPLOT v2.3 alatki); i predviĊanje funkcije pomoću alatki koje koriste nekoliko programa: JAFA, ProtFun 2.2 i GeneOntology. Glikobiohemijska analiza je obuhvatila ispitivanje uticaja CA125 antigena na adhezivnost i agregabilnost eritrocita, kao i moguće interakcije sa razliĉitim tipovima leukocitnih lektina tj. sigleka (Siglec - sialic acid-binding immunogobulin (Ig)-like lectin) i receptora sliĉnih lektinima tipa C (selektini, DC-SIGN - dendritic cell-specific ICAM-3-grabbing non-integrin 1, MMR - macrophage mannose receptor). Ispitivanje efekta CA125 antigena na eritrocite ĉoveka odreĊivano je u testovima na ĉvrstoj fazi ili primenom svetlosne mikroskopije. Interakcija CA125 antigena sa leukocitnim receptorima ispitivana je pomoću lektinskog- i imunoblota, kao i u testovima vezivanja i inhibicije na ĉvrstoj fazi, sa imobilisanim antigenima. Glikobiohemijskom analizom su, pored antigena iz ćelijske linije ovarijalnog karcinoma ĉoveka (clCA125), bili obuhvaćeni i antigen kancerskog porekla iz pleuralne teĉnosti (pfCA125) kao i antigen fetalnog porekla (pCA125), koji se sintetiše tokom trudnoće. Rezultati in silico analize proteinske sekvence MUC16/CA125, stavili su ovaj molekul u kontekst modularnih proteina sa anotiranom ulogom u adheziji i srodnim procesima. Uvid u sekvence koje pokazuju sliĉnost sa sekvencom MUC16/CA125, pokazao je da se one, uglavnom, nalaze u ekstracelularnom regionu MUC16, koji je bogat serinom i treoninom. Ove sliĉnosti se nisu mogle povezati sa anotiranim domenima iz dostupnih baza podataka, osim parcijalno, ali statistiĉki znaĉajno sa BLLF1, multidomenom karakteristiĉnim za glikoproteine omotaĉa virusa iz familije Herpesviridae. Bioinformatiĉka analiza je ukazala i na moguću korelaciju funkcionalnih aktivnosti, koje se pripisuju domenima bogatim serinom/treoninom kod identifikovanih proteina u okviru ispitivanih taksona, i moguće funkcije CA125 antigena. Na osnovu dobijenih rezultata, uloga CA125 antigena u procesima adhezije bi mogla ukljuĉiti i interakcije posredstvom konzerviranih proteinskih sekvenci, odgovornih za jonski transport, i interakcije sa šećernim supstratima. Polazeći od mucinske prirode CA125 antigena, kao i fizioloških stanja u kojima je njegova koncentracija u serumu povećana, u prvom delu glikobiohemijske analize, ispitan je uticaj CA125 antigena na eritrocite ĉoveka. Dobijeni rezultati su ukazali da pCA125 i pfCA125 umereno povećavaju agregaciju eritrocita, i uspešno inhibiraju njihovu adheziju. Za razliku od njih, clCA125 je pokazao neznatan uticaj na modulaciju ovih osobina eritrocita. Sumarni rezultati drugog dela analize, koja se odnosila na interakcije sa leukocitnim receptorima, pokazali su da CA125, kao ligand, može stupiti u interakcije sa razliĉitim tipovima proteina koji vezuju sijalinsku kiselinu. Pokazano je da postoje specifiĉni obrasci vezivanja sigleka za ispitivane CA125 antigene, kojima se ovi antigeni, uspešnije, razlikuju u odnosu na njihovo poreklo, nego što se to ĉini na osnovu obrazaca vezivanja biljnih lektina, takoĊe specifiĉnih za sijalinsku kiselinu. Najuoĉljivija razlika je zapažena u odnosu na ligandni kapacitet CA125 antigena fetalnog porekla prema sigleku-7, prisutnom na dendritskim ćelijama, NK (natural killer cell) ćelijama, monocitima i CD8+ T ćelijama i clCA125 antigena kancerskog porekla prema sigleku-9 i sigleku-10, prisutnim na B ćelijama, NK ćelijama, monocitima, neutrofilima i CD8+ T ćelijama. Za razliku od sigleka, sva tri ispitivana selektina: L-selektin, E-selektin i P-selektin su, na dozno-zavisan naĉin, interagovali sa pfCA125 i u manjoj meri pCA125, ali ne i clCA125. Pored toga, zapaženo je i da je P-selektin reagovao na znatno nižim koncentracijama nego L- i E-selektin. Na osnovu uoĉenog visokog afiniteta P-selektina, CA125 antigen bi pre stupio u interakcije sa aktiviranim endotelnim ćelijama ili krvnim ploĉicama, na kojima je ovaj selektin prisutan, nego sa ćelijama na kojima su prisutni drugi tipovi selektina. Ispitivanje interakcije CA125 antigena i DC-SIGN, koji je eksprimiran na površini dendritskih ćelija (DC) i koji vezuje manan i visoko-manozne glikane kao i glikane sa terminalnim Lex/Ley/Lea/Leb antigenima, pokazalo je da on prepoznaje pCA125 i pfCA125 antigen. Rezultati inhibicije su ukazali da je vezivanje DC-SIGN za pCA125 zavisno od prisustva glikana bogatih manozom, za razliku od vezivanja pfCA125, koje je nezavisno od prisustva N- ili O-glikana, što bi moglo uticati na njegove diskretne receptorske funkcije. Manozni receptor makrofaga (MMR), koji je prisutan na makrofagama i DC i koji vezuje terminalnu manozu i fukozu i SLex, nije reagovao ni sa jednim od ispitivanih CA125 antigena. Receptori sliĉni lektinima tipa C imaju važnu ulogu u imunskom sistemu, u smislu posredovanja u interakcijama tipa ćelija-ćelija, tj. u meĊusobnom kontaktu leukocita ili u njihovom kontaktu sa endotelom, kao i u vezivanju patogena. Promene u glikozilaciji liganada za receptore sliĉne lektinima tipa C, direktno utiĉu na njihovu aktivnost i specifiĉnost i imaju važne posledice na razvoj, preživljavanje i reaktivnost ćelija krvnog i imunskog sistema. Rezultati ovoga rada ukazuju da bi razlike u strukturi/glikozilaciji CA125 antigena, koje se vezuju za posebna fiziološka i patološka stanja, mogle menjati njegov uticaj na ćelije krvnog sistema ĉoveka i imati znaĉajne biomedicinske posledice u razliĉitim mikrosredinama. Dalji uvid u prirodu i mehanizme multifunkcionalnosti CA125 antigena zahteva interdisciplinarni pristup baziran na kompleksnim interakcijama koje su posredovane razliĉitim strukturnim domenima." ]
[ "CA125 antigen, a well known tumour marker for serous ovarian cancer, is an extracellular part of the mucin 16 (MUC16) molecule. This antigen arises from proteolytic degradation of MUC16, a type I transmembrane protein, expressed in both embrional and adult tissue. The primary structure of MUC16 consists of three characteristic parts: an N-terminal region and a series of tandem repeats, which are located extracellularly, and a C-terminal region, which consists of the transmembrane part and a short cytoplasmic tail. The extracellular peptide is extensively glycosylated, and there is a potential phosphorylation site in the intracellular chain. Phosphorylation is a signal for proteolysis and release of the extracellular part of the molecule (CA125 antigen), which can be detected as such in various body fluids. The biological role of MUC16/CA125 is not yet understood. Previous investigations of MUC16 (membrane form) functions were generally based on in vitro cell model systems of ovarian cancer, whereas published data on the activity of CA125 antigen (soluble form) are very rare. The soluble form is a functional analog and competitor to the membrane form, and, in the circulation, it first contacts different types of normal or pathologically altered cells. However, there is no experimental evidence on its possible impact on their adhesion nor immunomodulatory properties. In this work, the modular organization of human CA125 antigen was analyzed bioinformatically and glycobiochemically, aiming at closer definition of the biological capacity and presentation of this molecule in the context of the human interactome. As part of a strategy to detect a possible biological role for MUC16/CA125, bioinformatics analysis has never been used in studies of this antigen. In addition, in the context of discrete biological functions, regardless of its great biomedical importance, there are no CA125 antigen, a well known tumour marker for serous ovarian cancer, is an extracellular part of the mucin 16 (MUC16) molecule. This antigen arises from proteolytic degradation of MUC16, a type I transmembrane protein, expressed in both embrional and adult tissue. The primary structure of MUC16 consists of three characteristic parts: an N-terminal region and a series of tandem repeats, which are located extracellularly, and a C-terminal region, which consists of the transmembrane part and a short cytoplasmic tail. The extracellular peptide is extensively glycosylated, and there is a potential phosphorylation site in the intracellular chain. Phosphorylation is a signal for proteolysis and release of the extracellular part of the molecule (CA125 antigen), which can be detected as such in various body fluids. The biological role of MUC16/CA125 is not yet understood. Previous investigations of MUC16 (membrane form) functions were generally based on in vitro cell model systems of ovarian cancer, whereas published data on the activity of CA125 antigen (soluble form) are very rare. The soluble form is a functional analog and competitor to the membrane form, and, in the circulation, it first contacts different types of normal or pathologically altered cells. However, there is no experimental evidence on its possible impact on their adhesion nor immunomodulatory properties. In this work, the modular organization of human CA125 antigen was analyzed bioinformatically and glycobiochemically, aiming at closer definition of the biological capacity and presentation of this molecule in the context of the human interactome. As part of a strategy to detect a possible biological role for MUC16/CA125, bioinformatics analysis has never been used in studies of this antigen. In addition, in the context of discrete biological functions, regardless of its great biomedical importance, there are no experimental data on the interactions of MUC16/CA125 with specific classes of receptors on cells of the blood system. The modular organization of MUC16/CA125, was analyzed in silico using data taken from the UniProtKB database, which refers to a molecule isolated from the human ovarian cancer cell line, OVCAR-3. Bioinformatic analysis included: determination of homology (using BLAST, Basic Local Alignment Search Tool Tool); investigation of patterns, structural motifs and conserved domains (using databases such as Bgee: a database for Gene Expression and Gene Ontology Evolution, CDD, ELM, BLOCKS, InterProScan and MyHits iProClass); determination of physical and chemical properties, globularity and deviations from the assumed tertiary structure (using ProtParam, ProtScale and GLOBPLOT v2.3 tools); and function prediction using tools that employ several programs: JAFA, ProtFun 2.2 and GeneOntology. Glycobiochemical analysis comprised examination of the influence of CA125 on adhesion and aggregation of erythrocytes, as well as on interactions with different types of leukocyte lectins, namely siglecs [sialic acid-binding immunogobulin (Ig)-like lectin] and C-type lectin-like receptors [selectins, dendritic cell-specific ICAM-3-grabbing non-integrin 1 (DC-SIGN) and macrophage mannose receptor (MMR)]. The effect of CA125 antigen on human erythrocytes was determined using solid phase tests and light microscopy. The interaction of CA125 with leukocyte receptors was investigated employing lectin-blot and immunoblot, as well as binding and inhibition solid phase assays. Cancer antigen isolated from human pleural fluid (pfCA125) and pregnancy-associated antigen (pCA125) were included in the glycobiochemical analysis in addition to antigen from the human ovarian carcinoma cell line (clCA125). The results of in silico analysis placed MUC16/CA125 in the context of modular proteins with an annotated role in adhesion-related processes. They pointed to similarities within extracellular serine/threonine rich regions of MUC16 to protein sequences expressed in evolutionarily distant taxa. No relation to annotated domains from available databases appeared, except for BLLF1, a multidomain characteristic of virus envelope glycoproteins of the Herpesviridae family. Bioinformatics analysis also pointed to a possible correlation between functional activities, which are attributed to serine/threonine rich domains of experimental data on the interactions of MUC16/CA125 with specific classes of receptors on cells of the blood system. The modular organization of MUC16/CA125, was analyzed in silico using data taken from the UniProtKB database, which refers to a molecule isolated from the human ovarian cancer cell line, OVCAR-3. Bioinformatic analysis included: determination of homology (using BLAST, Basic Local Alignment Search Tool Tool); investigation of patterns, structural motifs and conserved domains (using databases such as Bgee: a database for Gene Expression and Gene Ontology Evolution, CDD, ELM, BLOCKS, InterProScan and MyHits iProClass); determination of physical and chemical properties, globularity and deviations from the assumed tertiary structure (using ProtParam, ProtScale and GLOBPLOT v2.3 tools); and function prediction using tools that employ several programs: JAFA, ProtFun 2.2 and GeneOntology. Glycobiochemical analysis comprised examination of the influence of CA125 on adhesion and aggregation of erythrocytes, as well as on interactions with different types of leukocyte lectins, namely siglecs [sialic acid-binding immunogobulin (Ig)-like lectin] and C-type lectin-like receptors [selectins, dendritic cell-specific ICAM-3-grabbing non-integrin 1 (DC-SIGN) and macrophage mannose receptor (MMR)]. The effect of CA125 antigen on human erythrocytes was determined using solid phase tests and light microscopy. The interaction of CA125 with leukocyte receptors was investigated employing lectin-blot and immunoblot, as well as binding and inhibition solid phase assays. Cancer antigen isolated from human pleural fluid (pfCA125) and pregnancy-associated antigen (pCA125) were included in the glycobiochemical analysis in addition to antigen from the human ovarian carcinoma cell line (clCA125). The results of in silico analysis placed MUC16/CA125 in the context of modular proteins with an annotated role in adhesion-related processes. They pointed to similarities within extracellular serine/threonine rich regions of MUC16 to protein sequences expressed in evolutionarily distant taxa. No relation to annotated domains from available databases appeared, except for BLLF1, a multidomain characteristic of virus envelope glycoproteins of the Herpesviridae family. Bioinformatics analysis also pointed to a possible correlation between functional activities, which are attributed to serine/threonine rich domains of experimental data on the interactions of MUC16/CA125 with specific classes of receptors on cells of the blood system. The modular organization of MUC16/CA125, was analyzed in silico using data taken from the UniProtKB database, which refers to a molecule isolated from the human ovarian cancer cell line, OVCAR-3. Bioinformatic analysis included: determination of homology (using BLAST, Basic Local Alignment Search Tool Tool); investigation of patterns, structural motifs and conserved domains (using databases such as Bgee: a database for Gene Expression and Gene Ontology Evolution, CDD, ELM, BLOCKS, InterProScan and MyHits iProClass); determination of physical and chemical properties, globularity and deviations from the assumed tertiary structure (using ProtParam, ProtScale and GLOBPLOT v2.3 tools); and function prediction using tools that employ several programs: JAFA, ProtFun 2.2 and GeneOntology. Glycobiochemical analysis comprised examination of the influence of CA125 on adhesion and aggregation of erythrocytes, as well as on interactions with different types of leukocyte lectins, namely siglecs [sialic acid-binding immunogobulin (Ig)-like lectin] and C-type lectin-like receptors [selectins, dendritic cell-specific ICAM-3-grabbing non-integrin 1 (DC-SIGN) and macrophage mannose receptor (MMR)]. The effect of CA125 antigen on human erythrocytes was determined using solid phase tests and light microscopy. The interaction of CA125 with leukocyte receptors was investigated employing lectin-blot and immunoblot, as well as binding and inhibition solid phase assays. Cancer antigen isolated from human pleural fluid (pfCA125) and pregnancy-associated antigen (pCA125) were included in the glycobiochemical analysis in addition to antigen from the human ovarian carcinoma cell line (clCA125). The results of in silico analysis placed MUC16/CA125 in the context of modular proteins with an annotated role in adhesion-related processes. They pointed to similarities within extracellular serine/threonine rich regions of MUC16 to protein sequences expressed in evolutionarily distant taxa. No relation to annotated domains from available databases appeared, except for BLLF1, a multidomain characteristic of virus envelope glycoproteins of the Herpesviridae family. Bioinformatics analysis also pointed to a possible correlation between functional activities, which are attributed to serine/threonine rich domains ofproteins identified in the studied taxa, and possible CA125 antigen function. Based on these results, the role of CA125 antigen in adhesion processes could involve interactions through conserved protein sequences responsible for ion transport, and for interactions with sugar substrates. Considering the mucin nature of CA125 antigen, as well as physiological conditions in which its concentration in serum is increased, the effect of this antigen on human erythrocytes was investigated in the first part of glycobiochemical analysis. The results showed that pCA125 and pfCA125 moderately increased aggregation of erythrocytes, and successfully inhibited their adhesion. In contrast, clCA125 showed little to no modulation of these properties. The second part of the glycobiochemical analysis related to interactions with leukocyte receptors. This showed that, as a ligand, CA125 may interact with different types of proteins that bind sialic acid. Siglecs were found to have specific binding patterns, which more effectively distinguished CA125 antigens of fetal from those of cancer origin, in contrast to the binding patterns of sialic acid-specific plant lectins. The most obvious difference was the ligand capacity of pCA125 antigen towards siglec-7, expressed on dendritic cells, NK (natural killer cells), monocytes and CD8+ T cells, and clCA125 towards siglec-9 and siglec-10 on B cells, NK cells, monocytes, neutrophils and CD8+ T cells. Unlike siglecs, all three investigated selectins: L-selectin, E-selectin and P-selectin, interacted in a dose-dependent manner with pfCA125, and to a lesser extent with pCA125, but not with clCA125. Moreover, P-selectin reacted at significantly lower concentrations than L- or E-selectin. Thus, CA125 antigen would preferentially interact with activated endothelial cells or platelets expressing P-selectin, compared to cells expressing the other types of selectin. The interaction of CA125 antigen and DC-SIGN, which is specific for mannan, high-mannose glycans and glycans with terminal Lex / Ley / Lea / Leb antigens was studied. DC-SIGN is expressed on dendritic cells (DC) and recognized pCA125 and pfCA125 antigen. Inhibition of DC-SIGN binding to pCA125 was dependent on the presence of mannose-rich glycans, in contrast to the binding to pfCA125, which was not affected by the presence of N- or O-linked glycans, all which can modify their discrete receptor functions. Macrophage mannose receptor (MMR), which is expressed on macrophages and DC, and which binds terminal mannose, fucose and sialyl Lex, did not react with any of the tested CA125 antigens. C-type lectin-like receptors play an important role in the immune system, mediating in cell-cell interactions, i.e. contacts between leukocytes or between leukocytes and endothelium,and in the binding of pathogens. Glycosylation changes in ligands for C-type lectin-like receptors directly influence their activity and specificity and have important effects on the development, survival and reactivity of cells of the blood and immune systems. Based on the results of this study, differences in the structure/glycosylation of CA125 antigens associated with different physiological or pathological conditions, could alter their influence on the human blood cells, and have important biomedical implications in different microenvironments. Further insight into the nature and mechanisms of CA125 multifunctionality requires an interdisciplinary approach, based on complex interactions that are mediated by its different structural domains." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173010/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "CA125 antigen", "in silico analiza", "glikobiohemijska analiza", "eritrociti", "adhezija", "leukocitni receptori", "sigleci", "selektini", "DC-SIGN", "manozni receptor makrofaga" ]
[ "CA125 antigen", "in silico analysis", "glycobiochemical analysis", "erythrocytes", "adhesion", "leukocyte receptors", "siglecs", "selectins", "DC-SIGN", "macrophage mannose receptor" ]
[ "Bioinformatička i glikobiohemijska analiza modularne organizacije CA125 antigena čoveka", "Bioinformatic and glycobiochemical analysis of human CA125 antigen modular organization" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Кораћ, Aлександра; Милутиновић, Бојана; Јанковић, Мирослава; Јанковић, Мирослава; Кораћ, Aлександра; Митић, Нинослав М.; Биоинформатичка и гликобиохемијска анализа модуларне организације ЦA125 антигена човека; Биоинформатичка и гликобиохемијска анализа модуларне организације ЦA125 антигена човека;
[ "https://nardus.mpn.gov.rs/bitstream/id/2086/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2086/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2088" ]
10.2298/bg20121109mitic
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/9890
123456789-9890.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:18245/bdef:Content/download
no
Biogeografska i ekološka studija flore briofita Srbije
Biogeographic and ecological study of bryophyte flora of Serbia
[ "Sabovljević, Marko" ]
[ "Veljić, Milan", "Grdović, Svetlana" ]
Pantović, Jovana P.
2018-09-20T09:54:34
[ "2018-09-20T09:54:34", "2020-07-03T08:07:09" ]
2018-05-09
[ "https://nardus.mpn.gov.rs/handle/123456789/9890", "http://eteze.bg.ac.rs/application/showtheses?thesesId=5995", "https://fedorabg.bg.ac.rs/fedora/get/o:18245/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50433807" ]
[ "Briološka istraživanja u Srbiji su istorijski nedovoljno zastupljena. Čak 25.559 literaturnih i herbarskih podataka o briofitama zabeleženim u Srbiji, od početka istraživanja briofita u Srbiji pa do danas, sakupljeno je u novoformiranu Bryo bazu. Prikupljeni podaci poslužili su za obimne komparativne biogeografske i ekološke analize. Brioflora Srbije broji 820 taksona (1 rožnjača, 143 jetrenjače i 686 mahovina). Od toga, 17 taksona je isključeno sa liste vrsta Srbije, a još 7 su označeni kao sumnjivi taksoni čije prisustvo u Srbiji je malo verovatno ili nepotvrđeno. Za svaki takson izrađene su mape rasprostranjenja u Srbiji. Analizom podataka utvrđeno je da je skoro polovina taksona briofita u Srbiji zabeleženo na manje od 5 lokaliteta, a čak 18% ima samo lokalno potvrđeno rasprostranjenje. Najbogatiji vrstama su regioni Istočne, Zapadne i Centralne Srbije, a najsiromašniji su Bački i Pomoravski region. Analizom distribucije po administrativnim okruzima i UTM 10x10 kvadratima jasno je da prostor Srbije nije ujednačeno istraživan, a kao prioritet za dalja briološka istraživanja izdvojeno je 6 regiona i 8 okruga. Analiza spektara areal tipova pokazuje veliku sličnost sa biogeografskim spektrom briofita na Balkanskom poluostvu. Detaljnijom biogeografskom analizom uočavaju se posebnosti brioflore različitih delova Srbije. Razmatrani ekološki faktori dodatno ističu specifičnosti pojedinih oblasti Srbije, pojašnjavaju prisustvo određenog taksona, ali i omogućavaju predikciju briotaksona na neistraženim područjima Srbije. Listaste jetrenjače preferiraju donekle umerenija staništa u odnosu na grupu talusnih jetrenjača koje su češće u manje gostoljubivim uslovima. U poređenju sa mahovinama, jetrenjače preferiraju (mikro)staništa sa manjom prosečnom godišnjom temperaturom." ]
[ "Bryological exploration in Serbia has been historically neglected. All together 25.559 literature and herbarium data on bryophytes recorded in Serbia, from the beginning of the exploration up to date, were collected in the newly formed Bryo database. Data collection was used for extensive comparative biogeographical and ecological analyses. The bryoflora of Serbia numbers 820 taxa (1 hornwort, 143 liverworts and 686 mosses). Out of this, 17 taxa were excluded from the checklist of Serbia, and another 7 were determined as doubtful taxa whose presence in Serbia is unlikely or unverified. For each taxa, the distribution maps in Serbia were made. Data analyses showed that almost half of the Serbian taxa were recorded in less than 5 localities, and 18% have only local occurrence. Eastern, Western and Central Serbia are the regions richest in bryophyte species, while the poorest are regions of Bačka and Pomoravlje. Distribution analyses by counties and UTM 10x10 km squares showed that the territory of Serbia is unequally bryologically investigated. Hence, 6 regions and 8 counties due to any or very few bryophyte records should be high priority for further bryological research. The analyses of the areal type spectra showed a great similarity to the biogeographical spectrum of the Balkan bryophyte flora. A more detailed biogeographical analysis revealed the specific features of the bryoflora in various parts of Serbia. The studied ecological factors additionally emphasize the peculiarities of certain areas of Serbia, also in terms of species composition. These allow prediction of bryotaxa in unexplored areas as well. Leafy liverworts prefer somewhat milder habitats compared to a group of talus liverwort species, which are more often found in harsh conditions. Compared to sister moss group, liverworts prefer (micro)habitats with the lower average annual temperatures." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "rožnjače", "jetrenjače", "mahovine", "istorijski pregled", "distribucija", "bogatstvo", "areal tipovi" ]
[ "hornworts", "liverworts", "mosses", "historical overview", "distribution", "richness", "areal types" ]
[ "Biogeografska i ekološka studija flore briofita Srbije" ]
[ "Biogeographic and ecological study of bryophyte flora of Serbia" ]
doctoralThesis
[ "BY-NC-ND" ]
Сабовљевић, Марко; Вељић, Милан; Грдовић, Светлана; Пантовић, Јована П.; Биогеографска и еколошка студија флоре бриофита Србије; Биогеографска и еколошка студија флоре бриофита Србије;
[ "https://nardus.mpn.gov.rs/bitstream/id/1542/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1543/IzvestajKomisije17572.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1542/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1543/IzvestajKomisije17572.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_9890" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/7989
123456789-7989.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:15354/bdef:Content/download
no
Rasprostranjenje, ekologija i centri diverziteta slepih miševa (Mammalia, Chiroptera) u Srbiji
Distribution, ecology and centres of bat diversity (Mammalia, Chiroptera) in Serbia
[ "Stamenković, Srđan" ]
[ "Stamenković, Srđan", "Ćetković, Aleksandar", "Šinžar-Sekulić, Jasmina", "Milošević-Zlatanović, Svetlana" ]
Paunović, Milan M.
2017-04-29T21:48:42
[ "2017-04-29T21:48:42", "2020-07-03T08:06:38" ]
2016-09-28
[ "https://nardus.mpn.gov.rs/handle/123456789/7989", "http://eteze.bg.ac.rs/application/showtheses?thesesId=4902", "https://fedorabg.bg.ac.rs/fedora/get/o:15354/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=48840975" ]
[ "U poslednjih šezdeset godina slepi miševi u Srbiji su veoma često bili objekti kraćih i/ili dugotrajnijih istraživanja, a rezultati su do sada samo sporadično objedinjavani i objavljivani. Cilj ove disertacije je objedinjavanje podataka o fauni slepih miševa na području Srbije, analiza i vrednovanje dominantnih ekoloških faktora koji generišu distribuciju faune, karakterizacija preferiranih tipova staništa i skloništa, analiza aktuelnog rasprostranjenja vrsta, analiza strukture faune i identifikacija centara diverziteta, procena statusa ugroženosti i predlog mera zaštite vrsta u Srbiji. Slepi miševi su po nizu ekološko-zoogeografskih pokazatelja među najugroženijim i najmanje poznatim sisarima, te je sumiranje saznanja, analiza i interpretacija podataka o rasprostranjenju preduslov za utvrđivanje ekoloških i zoogeografskih determinanti njihove raznovrsnosti kao osnova za izradu strategije očuvanja i zaštite vrsta, staništa i skloništa slepih miševa u Srbiji. U radu je izvršeno i definisanje areala svake pojedinačne vrste u Srbiji, utvrđivanje obrazaca vertikalne i horizontalne distribucije vrsta u odnosu na dominantne ekološke gradijente, definisanje karakterističnih bionomskih pokazatelja za svaku vrstu, utvrđivanje preferencije staništa i skloništa vrsta po fenološkim fazama tokom životnog ciklusa, procena brojnosti i populacionih trendova vrsta u Srbiji, analiza diverziteta slepih miševa Srbije po prostornim jedinicama, definisanje ekološkog statusa vrsta u Srbiji i nova procena statusa ugroženosti prema IUCN standardima i predlaganje mera očuvanja i zaštite vrsta, staništa i skloništa. Rasprostranjenje trideset vrsta slepih miševa, za koje je utvrđeno da žive u Srbiji, je mapirano u kvadratnim poljima osnove 10 km u okviru UTM projekcione mreže, i to: Rhinolophus hipposideros, Rh. ferrumequinum, Rh. euryale, Rh. mehelyi, Rh. blasii, Myotis daubentonii, M. dasycneme, M. capaccinii, M. brandtii, M. mystacinus, M. alcathoe, M. nattereri, M. emarginatus, M. bechsteinii, M. myotis, M. oxygnathus, Nyctalus noctula, N. leisleri, Pipistrellus pipistrellus, P. pygmaeus, P. nathusii, P. kuhlii, Hypsugo savii, Vespertilio murinus, Eptesicus serotinus, Barbastella barbastellus, Plecotus auritus, P. macrobullaris, P. austriacus i Miniopterus schreibersii. Liste nalaza su date za svaku vrstu u okviru kojih i svi prikupljeni podaci o jedinkama, kolonijama, datumu, nalazištu, staništu, skloništu, nadmorskoj visini, UTM kvadratnom polju, kolektorskom/inventarskom broju, ustanovi gde se dokazni primerak ili podatak o nalazu čuva, nalazaču, identifikatoru i eventualnoj litaraturnoj jedinici koja opisuje nalaz. Broj nalaza varira u okviru vrsta, i to od jednog (Plecotus macrobullaris) do 325 (Rhinolophus ferrumequinum). Za svaku vrstu posebno su dati opšti bionomski podaci, populacioni status, faktori ugrožavanja, zakonska zaštita, nacionalni i međunarodni status zaštite, te mere očuvanja. Tako prikazani podaci su sintetizovani, analizirani i vrednovani. Analiza rasprostranjenja nalazišta vrsta slepih miševa u Srbiji vršena je najpre po UTM kvadratnim poljima, po administrativnim celinama i po širim hipsometrijskim oblastima. Ukupno je registrovano 605 nalazišta. U UTM kvadratnim poljima je beleženo od jednog do osmadeset nalazišta. Od ukupno 2168 nalaza, među kojima dominiraju skloništa (70 %) i mesta preleta/koridori (20 %), 62 % je zabeleženo nakon 1990. godine. Na 126 nalazišta su registrovane porodiljske kolonije, na 273 zimske, a na 34 kopulatorne..." ]
[ "During the last sixty years bats have often been a subject of short- and long-term research but so far the results have been synthesised and even published only sporadically. The aim of this study is to integrate data on bat fauna of Serbia, to analyse and evaluate prevailing ecological parameters determining distribution of bat fauna, to determine preferred types of habitats and roost sites, to analyse present distribution of species, to analyse the composition of bat fauna and to identify centres of diversity, to asses conservation status and to propose measures for species protection in Serbia. According to number of ecological-zoogeographic indicators, bats are among the most vulnerable and least known mammals; therefore, summation of knowledge, analysis and interpretation of data on distribution, is prerequisite for identification of the ecological and zoogeographic determinants of bat diversity as a basis for development of the strategy for conservation and protection of bat species, habitats and roosts in Serbia. This study also: defines range of each species in Serbia, identifies patterns of vertical and horizontal distribution of species in relation to the dominant ecological gradients, defines characteristic bionomic indicators for each species, identifies species’ habitat and roost preference per phenophases, assesses abundance and population trends of species in Serbia, analyses bat diversity of Serbia by spatial units, defines ecological status of species in Serbia, presents new assessment of species’ conservation status according to IUCN system, proposes measures for conservation and protection of species, habitats and roosts. Distribution of thirty bat species which have been found in Serbia is mapped by 10x10 km squares of the UTM projection grid, namely: Rhinolophus hipposideros, Rh. ferrumequinum, Rh. euryale, Rh. mehelyi, Rh. blasii, Myotis daubentonii, M. dasycneme, M. capaccinii, M. brandtii, M. mystacinus, M. alcathoe, M. nattereri, M. emarginatus, M. bechsteinii, M. myotis, M. oxygnathus, Nyctalus noctula, N. leisleri, Pipistrellus pipistrellus, P. pygmaeus, P. nathusii, P. kuhlii, Hypsugo savii, Vespertilio murinus, Eptesicus serotinus, Barbastella barbastellus, Plecotus auritus, P. macrobullaris, P. austriacus and Miniopterus schreibersii. Findings are listed for each species, containing all the data on individuals, colonies, date, locality, habitat, roost site, altitude, UTM grid square, the collector / inventory number, institution keeping a voucher specimen or information on finding, collector name, identifier name and possible literature unit describing the finding. Number of sites varies between species – from single one (Plecotus macrobullaris) to 325 (Rhinolophus ferrumequinum). General bionomical data, population status, threats, legal protection, national and international status of protection as well as conservation measures are also given for each species. All those presented data are synthesised, analysed and evaluated. Distribution of bat findings in Serbia is analysed by UTM grid squares, first of all, and then by administrative units and by broader hypsometrical areas. A total of 605 sites are registered. One to eighty sites are recorded per single UTM grid squares. 62 % of the total 2,168 findings, mostly roosts (70 %) and flight paths / commuting routes (20 %), were recorded after 1990. At 126 sites nursery colonies are registered, at 273 winter colonies while at 34 sites mating colonies are found. The width of the species distribution range is analysed and it was found that only 4 out of 30 species have a narrow distribution..." ]
application/pdf
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Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Chiroptera", "rasprostranjenje", "Srbija", "status", "ekologija", "centri diverziteta", "očuvanje" ]
[ "Chiroptera", "distribution", "Serbia", "status", "ecology", "centres of diversity", "conservation" ]
[ "Rasprostranjenje, ekologija i centri diverziteta slepih miševa (Mammalia, Chiroptera) u Srbiji" ]
[ "Distribution, ecology and centres of bat diversity (Mammalia, Chiroptera) in Serbia" ]
doctoralThesis
[ "BY-NC-ND" ]
Стаменковић, Срђан; Милошевић-Златановић, Светлана; Ћетковић, Aлександар; Стаменковић, Срђан; Шинжар-Секулић, Јасмина; Пауновић, Милан М.; Распрострањење, екологија и центри диверзитета слепих мишева (Маммалиа, Цхироптера) у Србији; Распрострањење, екологија и центри диверзитета слепих мишева (Маммалиа, Цхироптера) у Србији;
[ "http://nardus.mpn.gov.rs/bitstream/id/1422/IzvestajKomisije8401.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1421/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1422/IzvestajKomisije8401.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1421/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_7989" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/2064
123456789-2064.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:3475/bdef:Content/download
no
Hipofizno-ovarijalni sistem pacova od fetalnog do peripubertalnog perioda života nakon intrauterinog izlaganja deksametazonu
Pituitary-ovarian axis of rats from fetal to peripubertal period of life after intrauterine exposure to dexamethasone
[ "Koko, Vesna" ]
[ "Milošević, Verica", "Nestorović, Nataša" ]
Ristić, Nataša M.
2016-01-05T11:46:19
[ "2016-01-05T11:46:19", "2020-07-03T08:08:35" ]
2012-11-05
[ "https://nardus.mpn.gov.rs/handle/123456789/2064", "http://eteze.bg.ac.rs/application/showtheses?thesesId=76", "https://fedorabg.bg.ac.rs/fedora/get/o:3475/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024526770" ]
[ "Tokom fetalnog razvića organizam prolazi kroz “kritične periode”, tokom kojih se dešavaju intenzivne ćelijske deobe, razvijanje i sazrevanje tkiva, organa i organskih sistema. Sredina u kojoj fetus raste i razvija se u velikoj meri odreñuje kvalitet razvojnog procesa, a svaka promena uslova dovodi do čitavog niza poremećaja, koji mogu biti praćeni smanjenjem fetalnog rasta ili IUGR (eng. Intrauterine growth retardation). Posledice IUGR su dugotrajne i ispoljavaju se u vidu različitih metaboličkih i kardiovaskularnih oboljenja u kasnijem životu. Fetalno okruženje na taj način odreñuje adultni fenotip, a njihova povezanost proučava se u okviru koncepta programiranja. Najčešće korišćeni eksperimentalni pristupi u proučavanju programiranja i efekta IUGR su dijeta majke koja vodi pothranjenosti, izazivanje stresne reakcije kod majke ili primena antenatalne terapije glukokortikoidima. Svaki od ovih eksperimentalnih modela različitim mehanizmima dovodi do izlaganja fetusa povećanoj koncentraciji glukokortikoida, koji u velikoj meri mogu da poremete fiziološki tok razvojnog procesa. Cilj ove studije je bio da se ustanovi da li je izlaganje fetusa pacova od 16. do 18.dana gestacije sintetičkom glukokortikoidu deksametazonu programiralo razvoj i funkciju hipofizno-ovarijalnog sistema od fetalnog do peripubertalnog perioda života. Predmet istraživanja u ovoj doktorskoj disertaciji bili su fetusi i ženke pacova koje su od 16. do 18. dana gestacije izloženi deksametazonu. Gravidne ženke pacova su tri uzastopna dana (od 16. do 18. dana gestacije) subkutano tretirane deksametazonom (Dexamethasonis phosphat - Dx, Krka, p.o., Novo Mesto) rastvorenom u fiziološkom rastvoru (0.9% NaCl), u dozi od 1.0, 0.5 i 0.5 mg Dx/kg..." ]
[ "During fetal development an organism undergoes “critical periods” of intensive cell division as well as the growth and maturation of tissues, organs and organ systems. The fetal environment principally determines the quality of the developmental process, while variation of conditions may lead to a range of disorders followed by intrauterine growth retardation (IUGR). The consequences of IUGR are long-lasting and manifest through various metabolic and cardiovascular issues in later life. Thus, the fetal environment prejudices the adult phenotype, while their intertwining is elaborated with the programming concept. The most widely exploited experimental approaches in the field of programming and IUGR effects are maternal diet leading to offspring malnutrition, provoking stress to mother and antenatal glucocorticoid therapy. Each of these experimental approaches via different mechanisms leads to fetal exposure to elevated glucocorticoid levels, which may disrupt to a great extent the physiology of the developmental process. The aim of this study was to establish whether the exposure of rat fetuses to synthetic glucocorticoid dexamethasone, during the period between days 16 and 18 of gestation, programmed the development and function of the pituitary-ovarian system, starting from the fetal to the peripubertal period of life. The subject of this doctoral thesis investigation were rat fetuses and females exposed to dexamethasone from the 16th to the 18th gestational day. During three consecutive days (16–18 days of gestation) pregnant rat females were subcutaneously treated with dexamethasone (Dexamethasonis phosphate – Dx, Krka, p.o., Novo Mesto) dissolved in saline (0.9% NaCl), in doses of 1.0, 0.5 and 0.5 mg Dx/kg..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173009/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "deksametazon", "fetusi", "potomci", "gonadotropne ćelije", "jajnik", "germinativne ćelije", "folikuli" ]
[ "dexamethasone", "fetus", "offspring", "gonadotropic cells", "ovary", "germinative cells", "follicles" ]
[ "Hipofizno-ovarijalni sistem pacova od fetalnog do peripubertalnog perioda života nakon intrauterinog izlaganja deksametazonu", "Pituitary-ovarian axis of rats from fetal to peripubertal period of life after intrauterine exposure to dexamethasone" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Коко, Весна; Несторовић, Наташа; Милошевић, Верица; Ристић, Наташа М.; Хипофизно-оваријални систем пацова од феталног до перипуберталног периода живота након интраутериног излагања дексаметазону; Хипофизно-оваријални систем пацова од феталног до перипуберталног периода живота након интраутериног излагања дексаметазону;
[ "https://nardus.mpn.gov.rs/bitstream/id/1913/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1913/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2064" ]
10.2298/bg20121105ristic
[ "" ]
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[ "" ]
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[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/10257
123456789-10257.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:18960/bdef:Content/download
no
Efekti inhibicije CXCR4 receptora i fokalne adhezione kinaze u supresiji invazije i prevazilaženju rezistencije kod nesitnoćelijskog karcinoma pluća
Effects of CXCR4 receptor and focal adhesion kinase inhibition in suppressing invasion and overcoming drug resistance in non-small cell lung carcinoma.
[ "Brajušković, Goran" ]
[ "Stanković, Tijana", "Dinić, Jelena" ]
Dragoj, Miodrag D.
2018-12-10T11:45:12
[ "2018-12-10T11:45:12", "2020-07-03T08:06:51" ]
2018-10-09
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6302", "https://nardus.mpn.gov.rs/handle/123456789/10257", "https://fedorabg.bg.ac.rs/fedora/get/o:18960/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025206962" ]
[ "Rak pluća je najčešće dijagnostikovani tip malignih tumora i vodeći uzrok smrti od malignih oboljenja. Na osnovu kliničke prognoze i odgovora na terapiju čak 85% raka pluća se svrstava u tip nesitnoćelijskog karcinoma pluća (NSCLC). Iako manje agresivan od sitnoćelijskog karcinoma pluća (SCLC), NSCLC lošije odgovara na terapiju. Dva najčešća uzroka neuspeha lečenja NSCLC-a klasičnom hemioterapijom su pojave metastaza i rezistencije na citostatike. Ovi procesi su regulisani brojnim signalnim molekulima, među kojima su i CXC hemokinski receptor 4 (CXCR4) i fokalna adheziona kinaza (FAK), čije ciljano inhibiranje predstavlja obećavajući pristup u lečenju NSCLC-a. Cilj ove studije je bio da se ispita uloga CXCR4 i FAK-a u invazivnosti i rezistenciji NSCLC-a, kao i potencijal inhibicije ovih molekula u supresiji invazije i reverziji rezistencije kod ovog tipa karcinoma pluća. Konkretno, za ispitivanje uloge CXCR4 i FAK-a u invazivnosti NSCLC-a uspostavljen je in vitro sistem ćelija sa različitim funkcionalnim statusom p53 i PTEN tumor supresora, kao i in vivo ortotopni metastatski model NSCLC-a sa p53/PTEN deficijentnim tumorima. U ispitivanom in vitro sistemu istovremena inaktivacija p53 i PTEN tumor supresora dovela je do aktivacije CXCR4 i FAK molekula i nishodnih signalnih molekula AKT-a i ERK-a, što je bilo praćeno povećanjem invazivnog i migratornog potencijala ćelija. Primenom specifičnih inhibitora CXCR4 i FAK-a, WZ811 i PF-573228, pokazan je potencijal primene inhibicije ovih molekula u supresiji invazije i migracije p53-/PTEN- NSCLC ćelija, kao i metastatskog širenja agresivnih p53/PTEN deficijentnih tumora u in vivo modelu. U daljem toku studije za ispitivanje uloge CXCR4 i FAK-a u razvoju rezistencije NSCLC-a na hemioterapiju korišćene su rezistentne ćelijske linije, NCIH460/ R i COR-L23, koje se odlikuju povećanom ekspresijom ABCB1, odnosno ABCC1 transportera. Pokazano je da kombinovani tretmani inhibitorima CXCR4 i FAK-a sa doksorubicinom (DOX) uspešno senzitizuju rezistentne ćelije na pomenuti citostatik. Ispitujući mehanizam reverzije rezistencije utvrđeno je da kombinovani tretmani smanjuju fosforilaciju FAK-a i AKT-a i dovode do aktivacije senescence-a. Istovremeno sa senzitizacijom ćelija na DOX, kombinovanim tretmanima je uspešno suprimirana i invazija rezistentnih ćelija..." ]
[ "Lung cancer is the most commonly diagnosed type of malignant tumor and the leading cause of cancer death. Based on clinical prognosis and terapy response, up to 85% of lung cancer is clasified as non-small cell lung carcinoma (NSCLC). Although NSCLC is less agresive than small cell lung carcinoma (SCLC), its response to terapy is worse then SCLC. Two major causes of terapy failure of NSCLC with classic chemotherapeutics are the development of metastasis and chemotherapy resistance. Among other signaling molecules, CXCR4 receptor and focal adhesion kinase (FAK) are known to regulate these processes and their targeted inhibition represents a promising approach in the treatment of NSCLC. The aim of this study was to examine the role of CXCR4 and FAK in the invasiveness and resistance of NSCLC, as well as the potential of their inhibition in the suppression of invasion and the reversal of resistance in this type of lung carcinoma. In order to examine the role of CXCR4 and FAK in the invasiveness of NSCLC, in vitro system of cancer cell lines with different functional status of the p53 and PTEN tumor suppressors was established, as well as in vivo orthoptic metastatic model of NSCLC with p53/PTEN deficient tumors. The simultaneous inactivation of p53 and PTEN tumor suppressors in in vitro system lead to significant increase in invasive and migratory potential associated with activation of CXCR4 and FAK molecules and their downstream signaling molecules, AKT and ERK. The potential for supressing invasion and migration of p53-/PTEN- NSCLC cells, as well as the metastatic spread of aggressive p53/PTEN deficient tumors in in vivo model, has been shown by the application of specific inhibitors of CXCR4 and FAK, PF-573228 and WZ811. Furthermore, resistant cell lines NCI-H460/R and COR-L23 were used to investigate the role of CXCR4 and FAK in the development of NSCLC resistance to chemotherapy. NCI-H460/R and COR-L23 cells are characterized by an increased expression of ABCB1 and ABCC1 transporters, respectively. Our results additionally indicate that inhibition of CXCR4 and FAK in combination with DOX successfully sensitized NSCLC cells to the aforementioned cytostatic. By examining the mechanism of sensitization of NSCLC cells to DOX, it has been discovered that combined treatments reduced phosphorylation of FAK and AKT and lead to activation of senescence. Simultaneously with sensitization of cells to DOX, combined treatment successfully suppressed the invasion of resistant cells..." ]
application/pdf
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Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41031/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]
Универзитет у Београду
[ "CXCR4", "FAK", "p53", "PTEN", "invazivnost", "doksorubicin", "rezistencija", "ABCB1", "ABCC1", "nesitnoćelijski karcinom pluća" ]
[ "CXCR4", "FAK", "p53", "PTEN", "invasion", "doxorubicin", "resistance", "ABCB1", "BACC1", "non-small cell lung carcinoma" ]
[ "Efekti inhibicije CXCR4 receptora i fokalne adhezione kinaze u supresiji invazije i prevazilaženju rezistencije kod nesitnoćelijskog karcinoma pluća" ]
[ "Effects of CXCR4 receptor and focal adhesion kinase inhibition in suppressing invasion and overcoming drug resistance in non-small cell lung carcinoma." ]
doctoralThesis
[ "BY-NC-SA" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1461/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1461/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1462/IzvestajKomisije18484.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1462/IzvestajKomisije18484.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10257" ]
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https://nardus.mpn.gov.rs/handle/123456789/11510
123456789-11510.pdf
yes
yes
https://fedorabg.bg.ac.rs/fedora/get/o:20287/bdef:Content/download
no
Mehanizmi antiencefalitogenog dejstva etil-piruvata u eksperimentalnom autoimunskom encefalomijelitisu
Antiencephalitogenic mechanisms of ethyl pyruvate in experimental autoimune encephalomyelitis
[ "Miljković, Đorđe" ]
[ "Božić, Biljana", "Lavrnja, Irena", "Miljković, Đorđe" ]
Đedović, Neda
2019-11-15T10:03:58
[ "2019-11-15T10:03:58", "2020-07-03T08:07:54" ]
2019-07-19
[ "https://nardus.mpn.gov.rs/handle/123456789/11510", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6927", "https://fedorabg.bg.ac.rs/fedora/get/o:20287/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025223602" ]
[ "Multipla skleroza (MS) je hronična, inflamacijska, demijelinizirajuća bolest centralnog nervnog sistema (CNS-a). Autoimunski odgovor usmeren protiv CNS-a je bitan element patogeneze MS-e. Eksperimentalni autoimunski encefalomijelitis (EAE) predstavlja životinjski model MS-e pomoću kog se istražuju patogenetski mehanizmi ove bolesti. Glavne ćelije koje učestvuju u pokretanju autoimunskog odgovora usmerenog protiv CNS-a su antigen prezentujuće ćelije (APĆ) koje aktiviraju naivne CD4+ T-ćelije specifične za antigene CNS-a. Ove CD4+ T-ćelije se potom diferenciraju u efektorske Th1 (engl. T helper cells – Th ćelije) koje imaju sposobnost produkcije interferona γ (IFN-γ) i Th17 koje produkuju interleukin 17 (IL-17). Prolaskom kroz-krvno-moždanu barijeru, Th1 i Th17 ćelije dolaze u CNS gde ih reaktiviraju rezidentne APĆ, te one sva.ojim produktima privlače druge imunske ćelije u CNS, što sve dovodi do inflamacije koja vodi oštećenju tkiva CNS-a. Patogenezi bolesti doprinose i rezidentne ćelije CNS-a kao što su astrociti i mikroglija. Etil-piruvat (EP) je lipofilni estar pirogrožđane kiseline koji poseduje antioksidativna i antiinflamacijska svojstva. U ovoj studiji je ispitivan antiencefalitogeni efekat EP-a na tok EAE-a i ćelije ukjučene u patogenezu EAE-a. Takođe, ispitivan je i njegov in vitro i in vivo tolerogeni uticaj na dendritske ćelije (DĆ). Rezultati su pokazali da EP ostvaruje terapijsko dejstvo na EAE kada se daje pacovima svakodnevno, počev od pojave prvih kliničkih simptoma bolesti sve do njihovog inicijalnog oporavka. Svoj antiencefalitogeni efekat EP je ispoljio sprečavanjem infiltracije imunskih ćelija u CNS, inhibicijom produkcije IL-17 od strane CD4+ T-limfocita u kičmenoj moždini, čime je sprečio zapaljensku reakciju u CNS-u. EP je doveo i do redukcije broja reaktivnih makrofaga i ćelija mikroglije, kao i do inhibicije reaktivnosti astrocita. Takođe, sprečio je i oštećenje neurona. Jedan od mehanizama kojim je EP ostvario svoje dejstvo je inhibicija HMGB1 (grupa proteina visoke mobilnosti 1 (engl. High-mobility group box 1) u reaktivnim makrofagima/mikrogliji. Da EP ima i druge efekte na APĆ pokazali su rezultati na makrofagima, in vitro. EP je redukovao produkciju proinflamacijskih citokina od strane makrofaga i ekspresiju molekula bitnih za prezentaciju antigena na ovim ćelijama. Zatim, istraživanje je prošireno na DĆ kao profesionalne APĆ. Rezultati su pokazali da EP vrši tolerogeni uticaj na DĆ poreklom iz kostne srži miševa..." ]
[ "Multiple sclerosis (MS) is a chronic, inflammatory, demyelinating disease of the central nervous system (CNS) with proposed autoimmune pathogenesis. Experimental autoimmune encephalomyelitis (EAE) is an animal model of MS that is widely used to investigate pathogenetic mechanisms of the disease. Antigen presenting cells (APC) are major cells that are involved in the initiation of the autoimmune response against the CNS by activating CNS-specific naive CD4+ T cells. Consequently, these CD4+ T cells differentiate into effector T helper cells 1 (Th1) that produce interferon γ (IFN-γ) and Th17 cells that produce interleukin 17 (IL-17). Passing through the blood brain barrier, Th1 and Th17 cells arrive in the CNS where they become reactivated by the resident APC. Afterwards, other immune cells infiltrate the CNS, thus causing inflammation and tissue damage. The resident cells of the CNS, such as astrocytes and microglia, also contribute to the disease pathogenesis. Ethyl pyruvate (EP) is a lipophilic ester of pyruvic acid that possesses anti-oxidative and anti-inflammatory properties. The anti-encephalitogenic effect of EP in EAE and on cells of the CNS involved in the disease pathogenesis, were investigated in this study. Also, its in vitro and in vivo tolerogenic effect on dendritic cells (DC) was studied. Results showed that EP had a therapeutic effect on EAE when applied to the rats once a day, starting from the first clinical symptoms until their initial recovery. EP prevented immune cells infiltration into the CNS and inhibited T cell production of IL-17 in the spinal cord. Thus, EP restrained the inflammatory reaction in the CNS and therefore exerted its anti-encephalitogenic effect. Furthermore, treatment with EP led to the reduction of macrophages and microglia cell number, inhibition of astrocyte activity, as well as neuron destruction. Inhibition of HMGB1 (High-Mobility Group Box 1) molecule in activated macrophages/microglia was one of the mechanisms of the EP effects in the CNS. Moreover, in vitro treatment of stimulated macrophages with EP showed that EP had also an impact on APC. Treatment with EP led to reduced production of pro-inflammatory cytokines by macrophages as well as to downregulation of the expression of molecules relevant for antigen presentation. Furthermore, EP had a tolerogenic effect on the major APC, i.e. DC..." ]
application/pdf
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Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173013/RS//", "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173035/RS//" ]
[ "openAccess" ]
[ "" ]
Универзитет у Београду
[ "eksperimentalni autoimunski encefalomijelitis", "multipla skleroza", "etil-piruvat", "tolerogene dendritske ćelije" ]
[ "experimental autoimmune encephalomyelitis", "multiple sclerosis", "ethyl pyruvate", "tolerogenic dendritic cells" ]
[ "Mehanizmi antiencefalitogenog dejstva etil-piruvata u eksperimentalnom autoimunskom encefalomijelitisu" ]
[ "Antiencephalitogenic mechanisms of ethyl pyruvate in experimental autoimune encephalomyelitis" ]
doctoralThesis
[ "ARR" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1726/IzvestajKomisije21121.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1725/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1726/IzvestajKomisije21121.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1725/Disertacija.pdf", "https://fedorabg.bg.ac.rs/fedora/get/o:20287/bdef:Content/download" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_11510" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/11507
123456789-11507.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:20265/bdef:Content/download
no
Bioindikatorske karakteristike epilitskih silikatnih algi i procena ekološkog statusa odabranih reka zapadne i centralne Srbije
Bioindicative characteristics of epilithic diatoms and ecological status assessment of selected rivers in western and central Serbia
[ "Krizmanić, Jelena" ]
[ "Levkov, Zlatko", "Subakov-Simić, Gordana", "Ćirić, Miloš", "Đorđević, Dragana" ]
Vidaković, Danijela
2019-11-15T10:03:57
[ "2019-11-15T10:03:57", "2020-07-03T08:07:50" ]
2019-07-01
[ "https://nardus.mpn.gov.rs/handle/123456789/11507", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6914", "https://fedorabg.bg.ac.rs/fedora/get/o:20265/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025220018" ]
[ "Silikatne alge su opšte prihvaćene kao indikatori procene stanja i promena uslova u vodenim ekosistemima. Osobine koje ih čine odličnim indikatorima su mnogobrojne: globalna rasprostranjenost, naseljavanje različitih mikrostaništa, prisutnost tokom cele godine, kratko vreme generacije individualnih populacija, njihove dobro poznate ekološke valence, standardizovani načini uzorkovanja, pravljenja trajnih preparata i njihovog čuvanja, identifikacija do nivoa vrste i jasno definisan način procene brojnosti. Prvi program monitoringa ekološkog statusa površinskih voda u Republici Srbiji, koji je usklađen sa zahtevima Okvirne direktive o vodama (ODV) započet je 2012. godine. Cilj ove doktorske disertacije je utvrđivanje diverziteta i sezonske dinamike epilitskih zajednica silikatnih algi, formiranje preliminarne liste bioindikatora epilitskih silikatnih algi, predlog potencijalnih korekcija odgovarajućih dijatomnih indeksa za teritoriju Republike Srbije i procena ekološkog statusa odabranih reka zapadne i centralne Srbije. Istraživanjem su obuhvaćene tri reke u zapadnoj Srbiji (Rača, Raška i Studenica) i jedna u centralnoj Srbiji (Rasina). Sakupljanje uzoraka je obavljeno šest puta u toku 2011. i 2012. godine, sa šest lokaliteta u rekama Rači, Rasini i Studenici, odnosno sa pet lokaliteta u reci Raški. Podaci neophodni za izračunavanje indikatorskih vrednosti silikatnih algi su dobijeni iz Agencije za zaštitu životne sredine Republike Srbije. Tokom istraživanog perioda najveći broj taksona u epilitskoj zajednici silikatnih algi je identifikovan u Rasini (251), zatim u Studenici (190), Rači (171), dok je u Raški zabeležen najmanji broj taksona (122). Gomphonema, Navicula i Nitzschia su dominantni rodovi u odnosu na broj taksona u svim istraživanim rekama. Od ukupnog broja identifikovanih taksona 69 je po prvi put zabeleženo za teritoriju Srbije. Među njima, Geissleria gereckei, Navicula splendicula i Navicula moskalii su taksoni koji su retko nalaženi u Evropi..." ]
[ "Diatoms are generally accepted as indicators for ecological status assessment and conditions change in aquatic ecosystems. Features that make them excellent indicators are numerous: worldwide distribution, colonization of different microhabitats, presence throughout the year, short generation time, known autecological characteristics, standardized sampling methods, making the permanent slides, their storage, identification to the level of the species and a clearly defined method of estimating the valve number. According to the Water Framework Directive (WFD), the first program for the monitoring of the ecological status of surface waters in the Republic of Serbia, started in 2012. The aim of this doctoral dissertation is determination of diversity and seasonal dynamics of epilithic diatoms, to form a preliminary list of epilithic diatom indicators, proposal of potential corrections of the appropriate diatom indices for the territory of the Republic of Serbia and ecological status assessment of selected rivers in Western and Central Serbia. The investigation includes three rivers in Western Serbia (Rača, Raška and Studenica) and one in Central Serbia (Rasina). Sampling was carried out six times during 2011 and 2012, on six sites in the rivers Rača, Rasina and Studenica and five in the Raška River. The data necessary for calculating diatom indicator values were obtained from the Serbian Environmental Protection Agency. During the investigated period, the highest number of taxa in the epilithic diatom community was recorded in the Rasina River (251), than in the Sudenica River (190), the Rača River (171), while the smallest number of taxa was recorded in the Raška River (122). The most abundant genera were Gomphonema, Navicula and Nitzschia. Of the total number of identified taxa 69 have been noted for the first time in Serbian diatom flora. Among them, Geissleria gereckei, Navicula splendicula and Navicula moskalii are taxa rarely found in Europe, too..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172001/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "epilitske silikatne alge", "dijatomni indeksi", "preliminarna lista indikatora", "TIDRS", "ekološki status", "Rača", "Rasina", "Raška", "Studenica" ]
[ "epilithic diatoms", "diatom idices", "preliminary list of indicators", "TIDRS", "ecological status", "Rača River", "Rasina River", "Raška River", "Studenica River" ]
[ "Bioindikatorske karakteristike epilitskih silikatnih algi i procena ekološkog statusa odabranih reka zapadne i centralne Srbije" ]
[ "Bioindicative characteristics of epilithic diatoms and ecological status assessment of selected rivers in western and central Serbia" ]
doctoralThesis
[ "BY-NC-ND" ]
[ "http://nardus.mpn.gov.rs/bitstream/id/1705/IzvestajKomisije21109.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1705/IzvestajKomisije21109.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1704/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1704/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_11507" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/2050
123456789-2050.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:2270/bdef:Content/download
no
Uloga angiotenzina II i reaktivnih vrsta kiseonika tokom razvoja akutne bubrežne insuficijencije u eksperimentalnoj hipertenziji
The role of angiotensin II and reactive oxygen species during the acute renal failure development in experimental hypertension
[ "Miloradović, Zoran" ]
[ "Đorđević, Jelena", "Mihailović-Stanojević, Nevena" ]
Ivanov, Milan S.
2016-01-05T11:46:08
[ "2016-01-05T11:46:08", "2020-07-03T08:07:12" ]
2012-07-28
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=25", "https://nardus.mpn.gov.rs/handle/123456789/2050", "https://fedorabg.bg.ac.rs/fedora/get/o:2270/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=41537039" ]
[ "Akutna bubrežna insuficijencija (ABI) se definiše kao nagli gubitak bubrežne funkcije. Uzroci nastanka ABI su mnogobrojni, a mehanizmi razvoja još uvek nedovoljno jasni. Karakterišu je iznenadna pojava, brzi tok, neizvesna i često loša prognoza krajnjeg ishoda bolesti. ABI je česta pojava kod hospitalizovanih pacijenata, a učestalost njenog nastanka na odeljenjima intenzivne nege se kreće do 30%. Udružena sa drugim oboljenjima, poput hipertenzije, uzrokuje visok stepen mortaliteta. Savremena istraživanja pokazuju da hipertenzija i oksidativni stres imaju važnu ulogu u progresiji bubrežnih oboljenja, što je usmerilo i stavilo akcenat naših istraživanja na uloge ova dva patogenetska faktora u nastanku i progresiji ABI. Sistem renin angiotenzin aldosteron (RAAS) ima značajnu ulogu u održanju krvnog pritiska i homeostatskih mehanizama bubrega. On u velikoj meri reguliše bubrežnu hemodinamiku i vaskularnu reaktivnost, a kako je intrarenalna vazokonstrikcija jedan od glavnih mehanizama razvoja ABI, očita je njegova uloga u patogenetskim mehanizmima nastanka ove bolesti. Oksidativni stres predstavlja disbalans između produkcije reaktivnih vrsta kiseonika (RVK) i sposobnosti biološkog sistema da ih uklanja ili da reparira oštećenja nastala produkcijom ovih molekula. Reaktivne vrste kiseonika su izuzetno reaktivne supstance usled postojanja nesparenih elektrona, i kao takve mogu da uzrokuju oštećenja na DNK, RNK, kao i nekim proteinima koji imaju ulogu u procesu starenja. Pokazano je da oksidativni stres ima veliku ulogu u razvoju mnogih oboljenja, kao što su Alchajmerova i Parkinsonova bolest, i neki kardiovaskularni poremećaji. Takodje je dokazano da, prilikom ishemičnih povreda organa i tkiva, nakon hipoksije, a tokom reperfuzije, dolazi do povećane produkcije molekula RVK. Iz pomenutih razloga, sasvim je izvesno da u ishemičnoj formi ABI povećana koncentracija ovih molekula, doprinosi nastanku i razvoju ovog teškog oboljenja..." ]
[ "Acute renal failure (ARF) is defined as a sudden loss of renal function. It’s multifactorially caused, but the mechanism of pathogenesis and developement of this disease is still uncomplitely defined. ARF is characterized by sudden appearance, rapid progression of disease and very uncertain and often fatal outcome. ARF frequently occurres with hospitalized patients. The frequency of its occurrence in the intensive care units moves up to 30%. Associated with other diseases, such as hypertension, it causes high rate mortality. Recent studies show that hypertension and oxidative stress have an important role in the renal disease progression. This fact directed the focus of our study towards the influence of these two pathogenetic factors in development and progression of ARF. The system rennin angiotensin aldosterone (RAAS) has a great role in blood pressure control and kidney homeostatic mechanisms. It mostly regulates the renal hemodynamic, but considering the fact that the intrarenal vasoconstriction is one of the major mechanisms of ARF development, it obviously influences the pathogenetic mechanisms in the development of this disorders. Oxidative stress is defined as a disbalance between the production of reactive oxygen species (ROS) and an ability of biological system to remove or fix the damage made due to production of these molecules. Reactive oxygen species are highly reactive due to presence of unpaired electrons and consequently lead to damage of DNA, RNA and some other ageing related proteins. There is evidence that the oxidative stress has a great influence in the progression of numerous diseases, such as Alzheimer’s, Parkinson’s, as well as some cardiovascular disorders. Furthermore, during the ischemia/reperfusion injuries of organs and tissues, there is increased reactive oxygen species molecule production. Thus, the ischemic type of ARF is characterized by a high concentration of these molecules which contribute to the development of this devastating illnes..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175096/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Akutna bubrežna insuficijencija", "Hipertenzija", "Renin angiotenzin aldosteron sistem", "Angiotenzin II", "Reaktivne vrste kiseonika", "Losartan", "Tempol", "Pacovi sa urodjenom hipertenzijom" ]
[ "Acute renal failure", "Hypertension", "Renin angiotensin aldosterone system", "Angiotensin II", "Reactive oxygen species", "Losartan", "Tempol", "Spontaneously hypertensive rat (SHR)" ]
[ "Uloga angiotenzina II i reaktivnih vrsta kiseonika tokom razvoja akutne bubrežne insuficijencije u eksperimentalnoj hipertenziji", "The role of angiotensin II and reactive oxygen species during the acute renal failure development in experimental hypertension" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Милорадовић, Зоран; Ђорђевић, Јелена; Михаиловић-Станојевић, Невена; Иванов, Милан С.; Улога ангиотензина ИИ и реактивних врста кисеоника током развоја акутне бубрежне инсуфицијенције у експерименталној хипертензији; Улога ангиотензина ИИ и реактивних врста кисеоника током развоја акутне бубрежне инсуфицијенције у експерименталној хипертензији;
[ "https://nardus.mpn.gov.rs/bitstream/id/1547/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1547/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2050" ]
10.2298/bg20120728ivanov
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https://nardus.mpn.gov.rs/handle/123456789/10260
123456789-10260.pdf
yes
yes
https://fedorabg.bg.ac.rs/fedora/get/o:18974/bdef:Content/download
no
Karakterizacija humanog mišićnog proteina Ankrd2
Characterization of human skeletal muscle protein Ankrd2
[ "Savić, Ana" ]
[ "Matić, Gordana", "Stefanović, Dragana", "Radović, Svetlana" ]
Kojić, Snežana
2018-12-10T11:45:16
[ "2018-12-10T11:45:16", "2020-07-03T08:06:42" ]
2003
[ "https://nardus.mpn.gov.rs/handle/123456789/10260", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6313", "https://fedorabg.bg.ac.rs/fedora/get/o:18974/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=26003471" ]
[ "Predmet istraživanja ovog rada je humani mišićni protein Ankrd2 koji se eksprimira u sporim mišićnim vlaknima i potencijalni je transkripcioni faktor čija je količina povećana u toku hipertrofije skeletnih mišića. To je protein od 37 kDa koji poseduje signal za lokalizaciju u jedru, četiri ankirinska ponovka i veoma je sličan jedarnom proteinu humanih endotelnih ćelija, C-193, čija je ekspresija indukovana citokinima. Pacovski i mišji ortologoni humanog proteina C-193, CARP odnosno MARP su pretežno eksprimirani u srcu i verovatno su uključeni u kontrolu hipertrofije srca. Pretpostavlja se da Ankrd2 u skeletnim mišićima ima sličnu funkciju kao CARP/MARP/C-193 u srcu. Za utvrdjivanje intraćelijske lokalizacije i ekspresije proteina Ankrd2 u različitim tkivima napravljena su tri mišja poliklonska antitela (na ceo protein, N-terminalni i C-terminalni region) i monoklonsko antitelo (na C-terminalni region proteina Ankrd2). Western blot analizom proteinskih ekstrakata različitih humanih tkiva pokazano je da se protein Ankrd2 eksprimira uglavnom u skeletnim mišićima i u manjoj meri u srcu i bubregu. U toku diferencijacije humanih (CHQ5B) i mišjih (C2C12) mišićnih ćelija dolazi do povećanja količine proteina Ankrd2. U cilju detaljne analize intraćelijske lokalizacije proteina Ankrd2 uradjeni su eksperimenti indirektne imunofluorescencije koristeći humane mioblaste i miotube. Generalno, u toku diferencijacije povećava se i broj ćelija koje fluoresciraju i intenzitet signala. Takodje, u humanim mioblastima, za razliku od miotuba, detektovana je specifična jedarna fluorescencija. Jedarni signal je u obliku tački i eksperimenti kolokalizacije su pokazali da se proteini Ankrd2 i PML nalaze u istim jedarnim strukturama nazvanim PML jedarna tela. U cilju izučavanja proteinsko-proteinskih interakcija pripremljeni su rekombinantni proteini fuzionisani sa GST markerom (F-Ankrd2 (5-333 ak), N-Ankrd2 (5-120 ak) i C-Ankrd2 (279-333 ak)) koji su korišćeni u GST „pull-down” eksperimentima. Ovom metodom je pokazano da protein Ankrd2 interaguje sa nekoliko, za sada neidentifikovanih, proteina. U aminokiselinskoj sekvenci proteina Ankrd2 nalaze se četiri potencijalna mesta za fosforilaciju kazein kinazom II (CKII). U ovom radu je pokazano da protein Ankrd2 može biti fosforilisan in vitro ovom kinazom, s tim što je samo četvrto mesto (SGRE, 318-321 ak) fosforilisano. Eksperimenima retardacije DNK na gelu utvrdjeno je da proteini Ankrd2 i IkB, inhibitor transkripcionog faktora NFkB, nemaju sličnu funkciju iako imaju strukturne sličnosti (oba proteina poseduju ankirinske ponovke, signal za lokalizaciju u jedru i mesta za fosforilaciju raznim kinazama). Za razliku od IkB, protein Ankrd2 ne sprečava formiranje kompleksa izmedju DNK i NFkB." ]
[ "The object of this study was the new human muscle protein Ankrd2 found preferentially in slow muscle fibers that is possibly a transcription factor up-regulated in hypertrophy. It is a protein of 37 kDa, which has a signal for nuclear targeting, four ankyrin repeat motifs and shows significant similarity to a cytokine inducible nuclear protein C-193 from human endothelial cells. The rat and mouse orthologs of human C-193 called respectively CARP and MARP are mainly expressed in heart and probably involved in the control of cardiac hypertrophy. It is possible that Ankrd2 may play a similar role to CARP/MARP/C-193, but in skeletal muscle rather than heart. For intracellular localization and tissue distribution of Ankrd2 three mouse polyclonal antibodies (raised against the full length, N-terminal and C-terminal regions of the protein) and one monoclonal antibody (raised against C-terminal region of Ankrd2) were made. Western blot analysis of protein extracts isolated from different human tissues showed that Ankrd2 is expressed mainly in human skeletal muscle and to a lesser extent in heart and kidney. During muscle cell differentiation there is an increase of Ankrd2 signal in both mouse (C2C12) and human (CHQ5B) muscle cells that can be detected by Western blot analysis. Immunofluorescence experiments were undertaken in primary human myoblasts and myotubes with the scope of pinpointing the intracellular localization of Ankrd2 protein. In general, during differentiation both the number of fluorescing cells and the intensity of signal increase. Also, in human myoblasts but not in differentiated myotubes very specific nuclear fluorescence can be detected. The nuclear signal is in the form of speckles and co-localization experiments showed that Ankrd2 and PML co-localize in PML nuclear bodies. In order to study protein-protein interactions GST recombinant proteins F-Ankrd2 (5-333 aa), N-Ankrd2 (5-120 aa) and C-Ankrd2 (279-333 aa) were prepared and used for the GST „pull-down” experiments. Results show that Ankrd2 interacts with several proteins, but so far these proteins are not identified. The deduced amino acid sequence of Ankrd2 contains four putative casein kinase II (CKII) phosphorylation sites. Ankrd2 can be phosphorylated in vitro with CKII, but only the fourth site (318-321 aa, SGRE) is phosphorylated. In order to discover wether Ankrd2 has a similar function to IkB, the inhibitor of transcriptional factor NFkB (as they have structural similarities (ankyrin repeats, nuclear localization signal, phosphorylation sites)) electromobility shift assays (EMSA) were performed. Ankrd2 does not prevent the formation of a complex between NFkB and its DNA binding site whereas IkB in the same experiments was able to do so, thus suggesting that Ankrd2 does not function as muscle specific IkB protein." ]
application/pdf
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Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "" ]
Универзитет у Београду
[ "мишић", "Ankrd2", "миобласт", "миотуба", "антитела", "PML једарна тела" ]
[ "muscle", "Ankrd2", "myoblast", "myotube", "antibodies", "PML bodies" ]
[ "Karakterizacija humanog mišićnog proteina Ankrd2" ]
[ "Characterization of human skeletal muscle protein Ankrd2" ]
doctoralThesis
[ "ARR" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1433/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1433/Disertacija.pdf", "https://fedorabg.bg.ac.rs/fedora/get/o:18974/bdef:Content/download" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10260" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/10343
123456789-10343.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:18997/bdef:Content/download
no
Efekti anestezije indukovane propofolom na sinaptičku plastičnost, aktivnost dopaminskog sistema i ponašanje juvenilnih pacova
The effect of propofol induced anesthesia on synaptic plasticity, dopaminergic system activity and behaviour of juvenile rats
[ "Pešić, Vesna" ]
[ "Dacić, Sanja", "Kanazir, Selma", "Pešić, Vesna" ]
Pavković, Željko Z.
2018-12-15T09:37:18
[ "2018-12-15T09:37:18", "2020-07-03T08:07:27" ]
2018-09-21
[ "https://nardus.mpn.gov.rs/handle/123456789/10343", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6327", "https://fedorabg.bg.ac.rs/fedora/get/o:18997/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025197746" ]
[ "Propofol je često korišćeni anestetik u modernoj medicini. Adiktivni potencijal propofola je uočen, kao i uticaj na memorijski proces. Međutim, oba fenomena su još uvek nedovoljno istražena. Imajući u vidu da je adolescencija period izuzetne osetljivosti na dejstvo adiktivnih supstanci i intenzivne maturacije mnemoničkog potencijala, cilj ovog rada je bio da se ispita uticaj jednokratnog izlaganja propofolskoj anesteziji, što je tipičan način kliničke primene anestetika, na dopaminsku signalizaciju, sinaptičku i neuronsku aktivnost u različitim regionima mozga i ponašanje juvenilnih/peripubertetskih pacova, kao model sistema humanog periadolescentnog razvoja. Efekti su analizirani 4, 24 i 48 sati nakon tretamana, kod mužjaka Wistar pacova starih 35 dana. Dobijeni rezultati su po prvi put ukazali da izlaganje propofolskoj anesteziji izaziva promene u ekspresiji/fosforilaciji signalnih molekula koji su već prepoznati kao značajni za dejstvo adiktivnih supstanci. Od svih analiziranih dopaminoceptivnih moždanih regiona (medijalni prefrontalni korteks, strijatum i talamus) jedino su u talamusu uočene značajne promene u ekspresiji fosforilisane/aktivirane forme DARPP-32 proteina, pokazatelja postsinaptičke dopaminske signalizacije, 4 i 24 sata nakon tretmana, i bile su praćene povećanom ekspresijom FosB/ΔFosB proteina, biohemijskog pokazatelja neuronske aktivnosti. Promene su lokalizovane u paraventrikularnom talamičkom jedru i mediodorzalnom talamusu. U strijatumu i medijalnom prefrontalnom korteksu je uočen porast u ekspresiji fosforilisane forme CaMKIIα, biohemijskog senzora sinaptičke aktivnosti koji ima važnu ulogu u pamćenju prethodnog izlaganja adiktivnim supstancama. Smanjenje u intenzitetu anksioznosti (procenjeno na osnovu rezultata dobijenih u testu svetlo/tamne kutije i uzdignutog krstastog lavirinta) je zabeleženo 24 sata nakon tretmana, kada je uočen i pad u ekspresiji FosB proteina u strijatumu, što se može tumačiti kao traženje senzacija usled smanjene aktivnosti moždanog regiona značajnog za osećaj zadovoljstva i motivisanost. Pojačan motorički odgovor na d-amfetamin i fenciklidin je uočen 24 sata nakon tretmana (ukrštena senzitizacija), kao potvrda da bez obzira na različite primarne mehanizme dejstva propofol i dve korišćene droge koriste iste neuronske puteve za ostvarivanje psihomotoričkih efekata..." ]
[ "Propofol is a commonly used anesthetic in modern medicine. Addictive potential of propofol is observed, as well as the impact on the memory process. However, both phenomena are still insufficiently explored. Bearing in mind that adolescence is a period of extreme sensitivity to addictive substances and intense maturation of the mnemonic potential, the aim of this study was to examine the effect of a single exposure to propofol anesthesia, which is a typical method of its clinical application, on dopaminergic signaling, synaptic and neuronal activity in different brain regions and behavior of juvenile/peripubertal rats, as a model system of human periadolescencent development. The effects were analyzed 4, 24 and 48 hours after the treatment, in male Wistar rats aged 35 days. The obtained findings for the first time showed that exposure to propofol anesthesia caused changes in the expression/phosphorylation of signal molecules that are already recognized as significant for the action of the addictive substances. Of all the analyzed dopaminoceptive brain regions (medial prefrontal cortex, striatum and thalamus), significant changes in the expression of the phosphorylated/activated form of DARPP-32 protein, indicator of postsynaptic dopaminergic signalling, were observed only in the thalamus, 4 and 24 hours after the treatment, and were accompanied by increased expression of FosB/ΔFosB protein, a biochemical indicator of neuronal activity. The alterations were localized in the paraventricular thalamic nucleus and the mid-dorsal thalamus. An increase in the expression of the phosphorylated form of CaMKIIα, a biochemical sensor of synaptic activity that has an important role in memory on addictive substances exposure, was detected in striatum and medial prefrontal cortex. Reduction in the intensity of anxiety (estimated in accordence to the data obtained in the light/dark box and elevated plus maze tests) was observed 24 hours after the treatment, along with the decrease in the expression of FosB protein in striatum, which can be interpreted as a sensation seeking due to decreased activity of brain region important for a pleasure/motivation..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173051/RS//", "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173056/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]
Универзитет у Београду
[ "propofol", "peripubertet", "zavisnost/adikcija", "anksioznost", "dopamin", "sinaptička aktivnost", "sinaptička stabilnost", "prostorna habituacija", "prepoznavanje novog objekta", "epizodična memorija" ]
[ "propofol", "peripuberty", "addication", "anxiety", "dopamine", "synaptic activity", "synaptic stability", "spatial habituation", "novel object recognition", "episodic memory" ]
[ "Efekti anestezije indukovane propofolom na sinaptičku plastičnost, aktivnost dopaminskog sistema i ponašanje juvenilnih pacova" ]
[ "The effect of propofol induced anesthesia on synaptic plasticity, dopaminergic system activity and behaviour of juvenile rats" ]
doctoralThesis
[ "BY-NC-SA" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1603/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1604/IzvestajKomisije18505.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1603/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1604/IzvestajKomisije18505.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10343" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/10255
123456789-10255.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:18922/bdef:Content/download
no
Анализа варијабилности митохондријске ДНК становника Републике Србије са популационо генетичког, филогенетског и филогеографског аспекта
Analysis of mitochondrial DNA variability of residents of the Republic of Serbia : population genetics, phylogenetic and phylogeographic perspectives.
[ "Kovačević-Grujičić, Nataša" ]
[ "Stevanović, Milena", "Stojković, Biljana", "Aleksić, Jelena" ]
Davidović, Slobodan B.
2018-12-10T11:45:10
[ "2018-12-10T11:45:10", "2020-07-03T08:06:40" ]
2018-09-24
[ "https://nardus.mpn.gov.rs/handle/123456789/10255", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6281", "https://fedorabg.bg.ac.rs/fedora/get/o:18922/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025198258" ]
[ "савременим људским популацијама Европе резултат су веома комплексних еволуционих, демографских и културолошких процеса који су се одвијали на простору Европе током више хиљада година. Међу најважнијим процесима издвајају се пост-глацијална реколонизација Европе, ширење пољопривреде током неолита (неолитска транзиција) и бројне миграције људских популација, попут миграција индоевропских народа током бронзаног доба и Велике сеобе народа у раном средњем веку. Балканско полуострво је имало важну улогу током целокупне историје људске врсте на евроазијском континенту због тога што је представљало важан миграцијски коридор преко којег су се одвијале миграције од праисторије па све до савременог доба. Такође, током последњег леденог максимума Балканско полуострво је представљало један од глацијaлних рефугијума са којег је отпочела пост-глацијaлна реколонизација Европе. Имајући у виду да српска популација насељава централни део Балканског полуострва, у овој популацији се могу очекивати генетички трагови комплексних демографских процеса. Предмет истраживања ове докторске дисертације било је испитивање варијабилности митохондријске ДНК (мтДНК) у савременој популацији Србије са популационо генетичког, филогенетског и филогеографског аспекта. У ту сврху анализирана је варијабилност првог и другог хиперваријабилног сегмента (HVS-I и HVS-II) и полиморфизми кодирајућег региона мтДНК код 172 испитаника чији су преци по женској линији из последње две генерације декларисаног српског етницитета. Поред тога, одабрано је и комплетно секвенцирано 58 митогенома који су анализирани у циљу бољег разумевања еволуције појединих мтДНК хаплогрупа, као и утврђивања доприноса различитих миграција у обликовању садашњег обрасца варијабилности митохондријског генског пула српске популације. У српској популацији заступљене су већином мтДНК хаплогрупе/подхаплогрупе карактеристичне за европске популације, а њихове учесталости и параметри генетичке разноврсности налазе сe у распону вредности детектованих у осталим европским популацијама. Српска популација није генетички диференцирана у односу на друге јужнословенске популације и у свим анализама заузима централну позицију између јужнословенских популација источног и западног дела Балканског полуострва. Највећи проценат HVS-I и HVS-I/HVS-II хаплотипова становништво Србије дели са географски блиским популацијама Балканског полуострва из јужнословенске групе, где су детектовани и потенцијално приватни хаплотипови..." ]
[ "Genetic structure and clinal distribution of genetic variation in contemporary human populations in Europe are the results of interplay between exceptionally complex evolutionary, demographic and cultural processes which took place in Europe during several thousand years. Among the most important processes are post-glacial recolonization of Europe, spread of farming during the Neolithic period (Neolithic transition) and numerous migrations of human populations such as Bronze Age migrations of Indo-Europeans and migrations during the Migration Period that occurred in the Early Middle Ages. The Balkan Peninsula played an important role during the entire history of humankind in Europe and Asia because it served as an important migration corridor starting from prehistoric to present days. Furthermore, during the last glacial maximum, this region was one of the glacial refugia from which the post-glacial recolonization of Europe was initiated. Since Serbian population inhabits the central part of the Balkan Peninsula, this population may harbour genetic traces of past complex demographic processes that were occurring in the Balkans over time. The subject of this doctoral thesis was the study of the mitochondrial DNA (mtDNA) variability in a contemporary population of Serbia from population genetics, phylogenetic and phylogeographic aspects. For that purpose, the variability of HVS-I and HVS-II regions and polymorphisms within the coding region of mtDNA have been assessed in 172 individuals whose Serbian maternal ancestry was confirmed for at least two generations. Furthermore, 58 selected mitogenomes were completely sequenced in order to provide new insights into the evolution of particular mtDNA haplogroups and to assess the contribution of various human migrations in modelling contemporary maternal gene pool of the Serbian population. Serbian population comprises mainly mtDNA haplogroups/subhaplogroups typical for European populations and their frequencies and levels of genetic diversity are mostly in the range of those detected in other European populations. Serbian population is not genetically differentiated from other south-Slavic populations and in all analyses it occupies a central position among south-Slavic populations from the eastern and western part of the Balkan Peninsula. The highest percentage of HVS-I and HVS-I/HVS-II haplotypes Serbian population shares with south-Slavic populations from the Balkan Peninsula, where potential private haplotypes have been observed as well. In general, European populations were grouped in a manner reflecting their geographic distribution. Furthermore, Slavic populations were grouped according to their linguistic affiliation. Rather high values of all parameters of genetic diversity of rare mtDNA subhaplogroups in the Balkan Peninsula are concordant with the previously demonstrated refugial character of this region..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Балканско полуострво", "српска популација", "мтДНК хаплогрупа", "Словени", "рефугијум", "генетичка разноврсност", "миграције" ]
[ "Balkan Peninsula", "Serbian population", "mtDNA haplogroup", "Slavs", "refugium", "genetic diversity", "migrations" ]
[ "Анализа варијабилности митохондријске ДНК становника Републике Србије са популационо генетичког, филогенетског и филогеографског аспекта" ]
[ "Analysis of mitochondrial DNA variability of residents of the Republic of Serbia : population genetics, phylogenetic and phylogeographic perspectives." ]
doctoralThesis
[ "BY-NC-ND" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1429/IzvestajKomisije18465.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1429/IzvestajKomisije18465.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1428/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1428/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10255" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/2151
123456789-2151.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:7903/bdef:Content/download
no
Uloga vanćelijskih proteina toplotnog stresa u inflamaciji povezanoj sa posttraumatskim stresnim poremećajem
The role of extracellular heat shock proteins on posttraumatic stress disorder-related inflammation
[ "Veličković, Nataša" ]
[ "Matić, Gordana", "Matić, Gordana", "Dunđerski, Jadranka" ]
Elzaedi, Younis M.
2016-01-05T11:48:19
[ "2016-01-05T11:48:19", "2020-07-03T08:07:24" ]
2013-12-27
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=1134", "https://nardus.mpn.gov.rs/handle/123456789/2151", "https://fedorabg.bg.ac.rs/fedora/get/o:7903/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024653490" ]
[ "Posttraumatski stresni poremećaj (PTSP) je psihijatrijska bolest koja se može razviti posle izlaganja traumatskom događaju. PTSP predstavlja veliko medicinsko i ekonomsko opterećenje za društvo, zbog relativno velike učestalosti i komorbiditeta sa mnogim drugim psihijatrijskim i somatskim bolestima. Smatra se da se najbolji rezultati u razumevanju patogeneze PTSP-a i njegovoj dijagnostici i terapiji mogu postići integrisanjem psiholoških, bioloških i farmakoterapeutskih pristupa. Aktuelna istraživanja sugerišu da je PTSP povezan sa neuroendokrinim poremećajima i promenama imunskih funkcija. Smatra se da je hiperosetljivost hipotalamo-hipofizno-adrenokortikalne (HHA) ose na kortizol neuroendokrini „pečat“ PTSP-a, dok je priroda imunskih poremećaja povezanih sa PTSP-om još uvek nejasna. Većina studija pokazala je da je PTSP povezan sa pojačanom inflamacijom, ali su objavljeni i podaci o nepromenjenom inflamatornom statusu, pa čak i smanjenom nivou inflamatornih markera u cirkulaciji PTSP pacijenata. Pretpostavlja se da pojačana inflamacija kod osoba obolelih od PTSP-a može biti posledica nedovoljne imunosupresije kortizolom. Međutim, podaci o nivou kortizola i funkcionisanju kortizolskih receptora kod pacijenata sa PTSP-om nisu konzistentni. Izlaganje različitim stresorima indukuje sintezu unutarćelijskih proteina toplotnog stresa (HSP) čije funkcije potpomažu preživljavanje ćelija. Tokom mnogo godina HSP su posmatrani kao unutarćelijski proteini, ali sada se zna da ih mnoge sisarske ćelije oslobađaju u cirkulaciju, gde mogu da aktiviraju pro-inflamatorne odgovore. Uzimajući da pojačana inflamacija koja je povezana sa PTSP-om verovatno nije posledica umanjenog anti-inflamatornog delovanja kortizola, mi smo pretpostavili da ona može biti posledica indukcije i oslobađanja Hsp60 i Hsp70 psihološkom traumom. Cilj ove studije bio je da se povežu parametri inflamatornog statusa sa nivoima kortizola i HSP u cirkulaciji, kao i da se otkriju moguće veze između inflamatornih markera i vanćelijskih HSP sa izlaganjem traumi, simptomima PTSP-a, osetljivošću i otpornošću na PTSP..." ]
[ "Post-traumatic stress disorder (PTSD) is a debilitating psychiatric disorder that may develop following exposure to life-threatening traumatic event. PTSD represents a serious medical and economic burden for the society due to its relatively high life-time prevalence rate and comorbidity with variety of other mental disorders and somatic illnesses. General opinion is that the best results in understanding of PTSD pathogenesis, diagnosis and treatment could be achieved by integrating psychological, biological and pharmacotherapeutical approaches. Current research has suggested that PTSD is associated with neuroendocrinological disturbances and immune function alterations. The hypersensitivity of hypothalamic-pituitary- adrenocortical (HPA) axis to cortisol feedback inhibition is considered the neuroendocrine hallmark of PTSD, while the nature of PTSD-related immune alterations is not well understood. Most studies report that PTSD is associated with excessive inflammation, but unaltered inflammatory state and even decreased circulatory levels of inflammatory markers were also reported. It has been hypothesized that excessive inflammation in individuals with PTSD might be a consequence of insufficient immunosuppression by cortisol. However, the data on cortisol levels and cortisol receptor functioning in PTSD patients are inconsistent. Exposure to a variety of stressors will induce intracellular heat shock proteins (HSPs) with cellular functions beneficial for cell survival. For many years HSPs have been viewed as intracellular proteins, but it is now known that they can be released from various mammalian cells into the peripheral circulation, where they are capable of activating pro-inflammatory responses. Taking that PTSD-associated excessive inflammation is not likely to be caused by impaired anti-inflammatory action of cortisol, we hypothesize that it might be a consequence of Hsp60 and Hsp70 induction and release by psychological trauma..." ]
application/pdf
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Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "posttraumatski stresni poremećaj", "proteini toplotnog stresa", "vanćelijski proteini toplotnog stresa", "citokini", "glukokortikoidni receptor", "kortizol" ]
[ "posttraumatic stress disorder", "heat shock proteins", "extracellular heat shock proteins", "cytokines", "glucocorticoid receptor", "cortisol" ]
[ "Uloga vanćelijskih proteina toplotnog stresa u inflamaciji povezanoj sa posttraumatskim stresnim poremećajem", "The role of extracellular heat shock proteins on posttraumatic stress disorder-related inflammation" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Величковић, Наташа; Дунђерски, Јадранка; Матић, Гордана; Матић, Гордана; Елзаеди, Yоунис М.; Улога ванћелијских протеина топлотног стреса у инфламацији повезаној са посттрауматским стресним поремећајем; Улога ванћелијских протеина топлотног стреса у инфламацији повезаној са посттрауматским стресним поремећајем;
[ "http://nardus.mpn.gov.rs/bitstream/id/1585/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1585/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2151" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/10351
123456789-10351.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:19035/bdef:Content/download
no
Prediktivni značaj prisustva aleksitimije u proceni anksioznosti i depresivnosti kod studenata obolelih od bronhijalne astme
Predictive role of alexithymia in the assessment of anxiety and depression in students with bronchial asthma
[ "Milenković, Branislava" ]
[ "Latas, Milan", "Šipetić Grujičić, Sandra", "Milovanović, Srđan", "Minić, Predrag" ]
Stojanović Ristić, Snežana
2018-12-19T16:02:44
[ "2018-12-19T16:02:44", "2020-07-03T08:45:49" ]
2018-09-28
[ "https://nardus.mpn.gov.rs/handle/123456789/10351", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6353", "https://fedorabg.bg.ac.rs/fedora/get/o:19035/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50780175" ]
[ "Astma kao jedna od najčešćih hroničnih bolesti utiče ne samo na fizičko nego i na mentalno zdravlje pacijenta. U mlađoj populaciji, uključujući i studente univerziteta, astma je od posebnog značaja, zbog više prevalencije u poređenju sa drugim hroničnim bolestima. Adolescenti sa astmom su u dva puta većem riziku za psihološke poremećaje kao što su anksioznost i depresija i u povećanom riziku za smrtni ishod zbod nje. Anksioznost i depresivnost su nezavisni faktori rizika za lošu kontrolu astme. Uloga aleksitimije, kao posebne specifične psihološke karakteristike opisane kao smanjene mogućnosti u prepoznavanju i opisivanju emocija, i povezanost sa nivoom anksioznosti i depresivnosti u astmi, takođe je prepoznata. Prevalencija aleksitimije je procenjena na 8-19% u opštoj populaciji, ali je primećena i kod obolelih od hroničnih bolesti. Aleksitimija može biti povezana sa rekurentnim veoma teškim egzacerbacijama kod obolelih od astme. Postoji mali broj istraživanja koja su pratila specifičnu ulogu aleksitimije u populaciji univerzitetskih studenta sa astmom. Cilj ove studije je bio da se odredi prevalencija aleksitimije kod studenata sa astmom, i njena relacija sa plućnom fumkcijom, anksioznošću i depresivnošću. Takođe, cilj je bio i validacija srpske verzije testa za kontrolu astme (ACT) kod obolelih od astme. Metod. Studija preseka je sprovedena u Zavodu za zdravstvenu zaštitu studenata u Beogradu, Srbija, i uključila je 210 studenata univerziteta sa astmom i 201 zdravog studenta kao kontrolnu grupu. Toronto aleksitimija skala (TAS-20) je korišćena za procenu aleksitimije i poređenje skorova tri glavna faktora aleksitimije: teškoće u prepoznavanju osećanja (Faktor 1), teškoće u opisivanju osećanja (Faktor 2) i eksterno orijentisano mišljenje (Faktor 3). Svi ispitanici su podvrgnuti spirometrijskom testiranju, procenjena im je kontrola astme korišćenjem Testa za kontrolu astme i na osnovu preporuka Globalne inicijative za astmu (GINE) i psihometrijski parametri su prezentovani Bekovom skalom depresivnosti (BDI) i Bekovom skalom anksioznosti (BAI). Psihometrijski skorovi izmereni ovim upitnicima su sumirani u Totalni psihometrijski skor (TPS) sa ciljem da se definiše odgovarajući psihološki poremećaj..." ]
[ "Asthma as one of the most prevalent chronic disease has impact not only on physical but also on mental health of the patient. In younger population, including university students, asthma is of special importance, having higher prevalence compared to other chronic diseases. Adolescents with asthma are at two-fold higher risk of psychological disorders such as anxiety and depression and are at increased risk for asthma death as well. Anxiety and depression are independant risk factors for poor asthma control. The role of alexithymia, as separate specific psychological feature described as lack of capability to identify and verbalize emotions, and association with levels of anxiety and depression in asthma has also been recognized. Prevalence of alexithymia is estimated to be 8-19% in general population, observed also in chronic diseases. Alexithymia may be related to recurrent very severe asthma exacerbations in patients with asthma. Limited research was published specifically at the role of alexithymia in university student population with asthma. The aim of this study was to determine prevalence of alexithymia in students with asthma, and its relationship with pulmonary function, anxiety and depression. Also, the aim was to validate Serbian version of the Asthma control test (ACT) in patients with athma. Method. The cross-section study was conducted in the Institute for Students Health Care in Belgrade, Serbia, including 210 university students diagnosed with asthma and 201 healthy students as control group. Toronto Alexithymia Scale (TAS-20) was used for alexithymia assessment and compared with scores for the three main alexithymia factors: difficulty identifying feelings (Factor 1), difficulty describing feelings (Factor 2) and externally-oriented thinking (Factor 3). All subjects underwent spirometry. Asthma control was assessed by Asthma Control test (ACT) score and Global Initiative for Asthma (GINA) guidelines and psychometric parameters presented by Beck’s Depression Index (BDI) and Beck’s Anxiety Index (BAI). All these parameters were analyzed for correlation. Psychometric scores obtained by these questionnaires were summed (Total Psychometric Score-TPS) with the aim to define an appropriate psychological disorder..." ]
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Универзитет у Београду, Медицински факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "astma", "aleksitimija", "anksioznost", "depresivnost", "Test kontrole astme", "studenti" ]
[ "Asthma", "Alexithymia", "Anxiety", "Depression", "Asthma Control Test", "students" ]
[ "Prediktivni značaj prisustva aleksitimije u proceni anksioznosti i depresivnosti kod studenata obolelih od bronhijalne astme" ]
[ "Predictive role of alexithymia in the assessment of anxiety and depression in students with bronchial asthma" ]
doctoralThesis
[ "BY-NC-ND" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/8120/IzvestajKomisije18528.pdf", "http://nardus.mpn.gov.rs/bitstream/id/8119/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/8120/IzvestajKomisije18528.pdf", "https://nardus.mpn.gov.rs/bitstream/id/8119/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10351" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/2623
123456789-2623.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:8556/bdef:Content/download
no
Тумачење права у оквиру Бетијеве херменеутике као универзалне методе духовних наука
Legal interpretation in the context of Betti's hermeneutics as universal method of Geisteswissenschaften
[ "Basta, Danilo" ]
[ "Hasanbegović, Jasminka", "Stojanović, Dragan" ]
Spaić, Bojan D.
2016-01-05T12:10:28
[ "2016-01-05T12:10:28", "2020-07-03T09:04:50" ]
2014-02-12
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=1392", "https://nardus.mpn.gov.rs/handle/123456789/2623", "https://fedorabg.bg.ac.rs/fedora/get/o:8556/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=512994224" ]
[ "Последње деценије XX века су и у оквиру филозофије, чији је основни проблем језик, довеле до преокрета који се назива интерпретативним окретом. У мноштву утицаја које је филозофија права претрпјела од аналитичке и континенталне традиције у филозофији једним су дијелом остали занемарени они утицаји који потичу од саме теоријске јуриспруденције, она правила која произилазе од саме практичке јуриспруденције, те се у неку руку и изгубила самосвојност коју је Келсен, изграђујући чисту теорију права, желио да подари правној науци. Централна тема највећег дијела докторске дисертације јесте управо херменаутика уопште и херменаутика једног италијанског правника посебно." ]
[ "The so-called interpretative turn has been a major development in the last two decades in philosophy. Among the variety of influences that the philosophy of law has experienced from analytical and phenomenological tradition, the influences derived from theoretical jurisprudence have remained neglected resulting in the dissolving of the self-contained character of legal science that Kelsen established by building his a pure theory of law. The core topic of this doctoral thesis is hermeneutics in general, with an emphasis on hermeneutics and legal hermeneutics developed by an Italian jurist and philosopher of law - Emilio Betti." ]
application/pdf
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Универзитет у Београду, Правни факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "hermeneutika", "tumačenje", "pravno tumačenje", "Emilio Beti", "kanoni tumačenja", "tekstualizam", "intencionalizam", "realizam", "skepticizam", "kognitivizam", "originalizam" ]
[ "heremenutics", "interpretation", "Emilio Betti", "legal interpretation", "canons of interpretation", "textualism", "intentionalism", "purposivism", "interpretative turn", "interpretivism", "realism", "skepticism", "cognitivism", "originalism", "living constitutionalism" ]
[ "Тумачење права у оквиру Бетијеве херменеутике као универзалне методе духовних наука", "Legal interpretation in the context of Betti's hermeneutics as universal method of Geisteswissenschaften" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Баста, Данило; Стојановић, Драган; Хасанбеговић, Јасминка; Спаић, Бојан Д.; Tumačenje prava u okviru Betijeve hermeneutike kao univerzalne metode duhovnih nauka;
[ "https://nardus.mpn.gov.rs/bitstream/id/13948/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/13948/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2623" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/10258
123456789-10258.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:18965/bdef:Content/download
no
Fitohemijska i morfološka diferencijacija prirodnih populacija Satureja montana L., S. kitaibelii Wierzb. ex Heuff. i S. subspicata Bartl. ex Vis. (Lamiaceae)
Phytochemical and morphological differentiation of natural populations of Satureja montana L., S. kitaibelii Wierzb. ex Heuff. and S. subspicata Bartl. ex Vis. (Lamiaceae)
[ "Marin, Petar D." ]
[ "Rajčević, Nemanja", "Vujisić, Ljubodrag", "Janaćković, Peđa", "Slavkovska, Violeta" ]
Dodoš, Tanja
2018-12-10T11:45:14
[ "2018-12-10T11:45:14", "2020-07-03T08:06:53" ]
2018-09-30
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6307", "https://nardus.mpn.gov.rs/handle/123456789/10258", "https://fedorabg.bg.ac.rs/fedora/get/o:18965/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50782479" ]
[ "Analizirana je morfološka i fitohemijska varijabilnost, kao i diferencijacija prirodnih populacija taksona roda Satureja (Lamiaceae) sa prostora Balkana. Morfološki karakteri su izmereni kod 366 individua iz 27 prirodnih populacija S. montana ssp. montana, S. montana ssp. variegata, S. montana ssp. pisidica, S. kitaibelii, S. subspicata ssp. subspicata, S. subspicata ssp. liburnica. Proučavano je 12 morfoloških karaktera (šest karaktera lista i šest čašice). Karakteri čašice su pokazali mnogo manju intrapopulacionu varijabilnost i bolje su odslikavali razlike između taksona, naročito podvrsta unutar vrste S. subspicata. Primenjene univarijante i multivarijantne statističke metode su pokazale različite stepene diferencijacije na različitim nivoima u zavisnosti od vrste. Diferencijacije populacija je pokazana kod S. kitaibelii i delimično kod S. subspicata. Dve podvrste S. subspicata se na osnovu morfoloških karaktera jasno odvajaju. U slučaju S. montana nisu uočene diferencijacije, ni na jednom nivou, zbog velike intrapopulacione varijabilnosti. Uočene su korelacije morfoloških karaktera i pojedinih bioklimatskih parametara, uglavnom vezanih za temperaturu i padavine. Takođe, kod S. subspicata postoji i pozitivna korelacija između morfoloških karaktera i geografskog regiona, što nije bio slučaj kod druge dve vrste. Određen je sastav i relativni udeo komponenti površinskih flavonoida izolovanih sa celih listova kod 108 individua iz 25 prirodnih populacija sa Balkana. Detektovano je 11, a identifikovano 10 jedinjenja. Kod populacija S. subspicata ssp. subspicata je bilo prisutno svih 11 jedinjenja, dok je u zavisnosti od populacije broj jedinjenja kod druge dve vrste varirao 5-10 (S. montana) i 7-10 (S. kitaibelii). U profilu S. montana u većini slučajeva dominiraju 5,6- diOH-7,3'4'-triMe flavon i cirzimaritin, kod S. kitaibelii ksantomikrol i cirzimaritin, a unutar S. subspicata ksantomikrol i cirzimaritin (ssp. liburnica), odnosno ksantomikrol i 8- metoksicirzilineol (ssp. subspicata). Udeo ksantomikrola je najveći kod populacija S. subspicata ssp. liburnica (skoro 60 %). Površinski flavonoidi, analizirani statističkim metodama, na celom setu podataka, pokazuju diferencijaciju između vrsta, uključujući i podvrste. Najviše se odvaja tipska podvrsta S. subspicata od svih ostalih taksona. Iako podvrsta S. montana ssp. montana pokazuje najveću varijabilnost, kontinentalne populacije ove podvrste se preklapaju sa populacijama S. kitaibelii s jedne, dok se primorske preklapaju sa populacijama S. montana ssp. variegata, s druge strane. Populacije S. subspicata ssp. liburnica se po sastavu flavonoida nalaze između ovih primorskih i kontinentalnih populacija. Uočena je korelacija sastava površinskih flavonoida i geografske distribucije populacija, jaka kod S. subspicata i S. kitaibelii, a slaba kod S. montana..." ]
[ "This thesis deals with morphological and phytochemical variability and differentiation of natural populations of taxa belonging to genus Satureja L. (Lamiaceae) (S. montana ssp. montana, S. montana ssp. variegata, S. montana ssp. pisidica, S. kitaibelii, S. subspicata ssp. subspicata and S. subspicata ssp. liburnica) from the Balkans. Morphological characters were measured in 366 individuals from 27 natural populations of selected taxa. In total 12 morphological characters were studied (six leaf and six calyx characters). Calyx characters varied less at the intrapopulation level and better reflected differences between the taxa, particularly between S. subspicata subspecies. Applied statistical methods (univariate and multivariate) showed diverse differentiation levels depending on the species studied. The separation between populations was observed in S. kitaibelii (complete) and in S. subspicata (partial). However, two S. subspicata subspecies were clearly differentiated. In the case of S. montana due to the high intrapopulation variability, no separation was found. Correlations between some of the morphological characters and some of the bioclimate parameters mostly related to temperature and precipitation were observed. Positive correlation between the morphological characters and the geographic region was shown only in S. subspicata. The surface flavonoids composition has been determined by HPLC and LC-MS analysis in 108 individuals from 25 natural populations. In total, 11 compounds were detected and 10 identified. All of them were present in S. subspicata ssp. subspicata, while in other two species number of compounds varied depending on the population studied (5-10 compounds in S. montana and 7-10 in S. kitaibelii). Surface flavonoids profile of S. montana, in most cases, was dominated by 5,6-diOH-7,3'4'-triMe flavone and cirsimaritin, while xanthomicrol and cirsimaritin dominated profiles of S. kitaibelii and S. subspicata ssp. liburnica. In S. subspicata ssp. subspicata xanthomicrol and 8-methoxycirsilineol were dominant. Xanthomicrol percentage was the highest in the S. subspicata ssp. liburnica (almost 60%). Flavonoids analyzed by statistical methods, in the whole dataset, showed differentiation among species including subspecies, particularly S. subspicata ssp. subspicata. Although, S. montana ssp. montana showed the highest variability of flavonoid composition, based on composition, continental populations of this subspecies overlap with S. kitaibelii populations, on one side, while coastal populations overlap with S. montana ssp. variegata populations, on the other. S. subspicata ssp. liburnica populations, based on flavonoid composition, were between those coastal and continental populations. Also, data showed a correlation between flavonoid composition and populations' geographical distribution – strong for S. subspicata and S. kitaibelii populations, and weak for S. montana..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173029/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Satureja montana", "S. kitaibelii", "S. subspicata", "Lamiaceae", "morfološki karakteri", "površinski flavonoidi", "n-alkani", "etarska ulja" ]
[ "Satureja montana", "S. kitaibelii", "S. subspicata", "Lamiaceae", "morphological characters", "surface flavonoids", "n-alkanes", "essential oils" ]
[ "Fitohemijska i morfološka diferencijacija prirodnih populacija Satureja montana L., S. kitaibelii Wierzb. ex Heuff. i S. subspicata Bartl. ex Vis. (Lamiaceae)" ]
[ "Phytochemical and morphological differentiation of natural populations of Satureja montana L., S. kitaibelii Wierzb. ex Heuff. and S. subspicata Bartl. ex Vis. (Lamiaceae)" ]
doctoralThesis
[ "BY-NC-ND" ]
[ "http://nardus.mpn.gov.rs/bitstream/id/1469/IzvestajKomisije18489.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1468/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1468/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1469/IzvestajKomisije18489.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10258" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/2091
123456789-2091.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:5438/bdef:Content/download
no
Komparativna analiza agenasa za biološku kontrolu patogenih gljiva izolovanih sa lekovitih biljaka
Comparative analysis of agents for biological of pathogenic fungi isolated from medicinal plants
[ "Berić, Tanja" ]
[ "Šavikin, Katarina", "Stanković, Slaviša", "Soković, Marina", "Gođevac, Dejan" ]
Stević, Tatjana
2016-01-05T11:46:47
[ "2016-01-05T11:46:47", "2020-07-03T08:09:29" ]
2013-04-23
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=192", "https://nardus.mpn.gov.rs/handle/123456789/2091", "https://fedorabg.bg.ac.rs/fedora/get/o:5438/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=43480335" ]
[ "Primena lekovitog bilja i njihovih preparata u prevenciji i lečenju različitih poremećaja u ljudskom organizmu može biti ograničena njihovom mogućom kontaminacijom fitopatogenim gljivama i mikotoksinima. Saznanja o riziku pri primeni hemijskih fungicida po rukovaoca, potrošača i životnu sredinu, dovela su do povećanja interesa za uvođenje alternativnih mera u zaštiti bilja, gde posebno mesto pripada preparatima prirodnog porekla tzv. agensima biološke kontrole. Biološka kontrola podrazumeva primenu korisnih mikroorganizama (bakterija, kvasaca, gljiva) ili produkata njihovog metabolizma, kao i primenu biljnih ekstrakata i etarskih ulja u zaštiti biljaka. Ispitivanjem preko 40 vrsta lekovitog bilja najlošiji mikrobiološki kvalitet utvrđen je za sledeće droge: kukuruznu svilu, list i herbu nane, list koprive, herbu rastavića i cvet nane. Iako su na svim biljnim drogama utvrđene mešovite infekcije gljivama iz različitih rodova, većina izolovanih vrsta gljiva pripada rodu Fusarium, a potom Aspergillus i Alternaria. Osim pomenutih, identifikovani su i predstavnici rodova: Penicillium, Phoma, Cephalosporium, Nigrospora, Cladosporium, Epicoccum, Gliocladium, Myrothecium, Cercospora, Phomopsis, Verticillum, Dreschlera (=Bipolaris), Rhizoctonia, Septoria, Trichoderma, Curvularia, Stahybotrys, Trichotecium, Puccinia, Botrytis, Mucor i Rhizopus sp., u zavisnosti od biljne droge. U cilju pronalaženja efikasnog biokontrolog agensa ispitivali smo mogućnost primene etarskih ulja i izolata Bacillus sp. u kontroli odabranih identifikovanih gljiva. U tom smislu, odabrali smo sledeće vrste gljiva: Fusarium solani, F. equiseti, F. oxysporum (izolovani sa kukuruzne svile i nevena), F. tricinctum, F. semitectum, F. sporotrichioides, F. subglutinans, Aspergillus flavus, A. niger, Alternaria alternata, Penicillium sp., Chaetomium sp., Curvularia lunata, Trichoderma viride, Trichotecium roseum, Gliocladium roseum, Myrotechium verrucaria, Phoma sp., Phomopsis sp. i Verticillium dahliae..." ]
[ "The application of medicinal plants and their preparations for the prevention and treatment of various disorders in humans may be limited by the possible contamination with phytopathogenic fungi and mycotoxins. Risk of using chemical fungicides for the operator, the consumer and the environment, have led to increasing interest in the introduction of alternative measures in plant protection. Lately, preparations of natural origin, so-called biological control agents are in the focus of investigation. Biological control involves the use of beneficial microorganisms (bacteria, yeasts, fungi) or the products of their metabolism, as well as the application of plant extracts and essential oils in plant protection. Examining over 40 stored dried medicinal plant species the lowest microbial quality were determined for next herbal drugs: Maydis stigmata (corn silk), Mentha leaf and herb (mint herb and leaf), Urtica leaf (nettle leaf), Equisetum herb (horsetail herb) and Calendula flower (marigold flower). Although mixed infections was recorded with different types of fungus the Fusarium was noted as the most dominant genera for most tested drugs, followed by Aspergillus and Alternaria. Twelve species of the genus Fusarium was identified. In addition, species from the following genera were identified: Phoma, Cephalosporium, Nigrospora, Cladosporium, Epicoccum, Gliocladium, Myrothecium, Cercospora, Phomopsis, Verticillum, Dreschlera (=Bipolaris), Rhizoctonia, Septoria, Trichoderma, Curvularia, Stahybotrys, Trichotecium, Puccinia, Botrytis, Mucor and Rhizopus sp. depending on plant species. In order to find an effective biological control agent, we investigated the possibility of applying the essential oils and isolates of Bacillus sp. in the control of selected identified fungi. In this regard, we chose the following fungal species: Fusarium solani, F. equiseti, F. oxysporum (isolated from corn silk and marigold flower), F. tricinctum, F. semitectum, F. sporotrichioides, F. subglutinans, Aspergillus flavus, A. niger, Alternaria alternata, Penicillium sp., Chaetomium sp., Curvularia lunata, Trichoderma viride, Trichotecium roseum, Gliocladium roseum, Myrotechium verrucaria, Phoma sp., Phomopsis sp. and Verticillium dahliae..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46013/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Fitopatogene gljive", "biološka kontrola", "etarska ulja", "antifungalna aktivnost", "izolati Bacillus sp.", "ekstrakti izolata antagonista", "antagonizam" ]
[ "Pathogenic fungi", "biological control", "essential oils", "antifungal activity", "Bacillus sp. isolates", "lipopeptide extracts of isolates", "antagonism" ]
[ "Komparativna analiza agenasa za biološku kontrolu patogenih gljiva izolovanih sa lekovitih biljaka", "Comparative analysis of agents for biological of pathogenic fungi isolated from medicinal plants" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Берић, Тања; Шавикин, Катарина; Соковић, Марина; Гођевац, Дејан; Станковић, Славиша; Стевић, Татјана; Компаративна анализа агенаса за биолошку контролу патогених гљива изолованих са лековитих биљака; Компаративна анализа агенаса за биолошку контролу патогених гљива изолованих са лековитих биљака;
[ "https://nardus.mpn.gov.rs/bitstream/id/2105/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2105/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2091" ]
10.2298/bg20130423stevic
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/10969
123456789-10969.pdf
yes
yes
https://fedorabg.bg.ac.rs/fedora/get/o:19450/bdef:Content/download
no
Karakterizacija bazalnog promotora humanog SOX3 gena i identifikacija regulatornih elemenata odgovornih za indukciju SOX3 gena retinoičnom kiselinom
Characterization of the basal promoter of the human SOX3 gene and identification of the regulatory elements responsible for the SOX3 gene induction by retinoic acid
[ "Stevanović, Milena" ]
[ "Romac, Stanka", "Radović, Svetlana" ]
Kovačević Grujičić, Nataša R.
2019-04-22T11:59:00
[ "2019-04-22T11:59:00", "2020-07-03T08:07:40" ]
2009
[ "https://nardus.mpn.gov.rs/handle/123456789/10969", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6637", "https://fedorabg.bg.ac.rs/fedora/get/o:19450/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=36878607" ]
[ "SOX3/Sox3 gen je jedan od najranijih neuralnih markera kod kičmenjaka, koji determiniše neuronalni tip ćelijske diferencijacije. Iako je SOX3 gen ključni regulator ranih faza embrionalnog razvića, do skora je u literaturi postojalo malo podataka o mehanizmima regulacije aktivnosti ovog gena. U ovoj tezi su, po prvi put, analizirani molekularni mehanizmi uključeni u transkripcionu regulaciju ekspresije SOX3 gena. Metodom elongacije reverznog oligonukleotida određen je start transkripcije ovog gena, 252 nukleotida uzvodno od ATG kodona. Funkcionalnom analizom definisan je minimalni promotorski region (-219/+67) neophodan za bazalnu transkripcionu aktivnost SOX3 gena u NT2/D1 ćelijama. Delecionom analizom minimalnog promotora identifikovana su dva cis regulatorna regiona, -219/-100 i +27/+67, koja su neophodna za aktivnost SOX3 promotora. Takođe, identifikovana su i dva regiona (-427/-219 i +67/+286) koji sadrže pozitivne cis regulatorne elemente neophodne, kako za optimalnu aktivnost, tako i za indukciju SOX3 promotora retinoičnom kiselinom. Dodatna funkcionalna analiza je ukazala da je fragment -427/-293 neophodan za inducibilnost promotora ovog gena. Takođe, poređenje sekvenci je pokazalo da su mesta za vezivanje Sp1, USF, NF-Y i CREB proteina, kao i TATA boks, očuvani i po nukleotidnom sastavu i po poziciji u minimalnim promotorskim regionima kod svih analiziranih SOX3 ortologa sisara. Funkcionalna analiza je po prvi put pokazala da su transkripcioni faktori Sp1, USF1, NF-Y, CREB i MAZ transkripcioni aktivatori ekspresije SOX3 gena. S druge strane, rezultati prikazani u ovoj tezi su ukazali da transkripcioni faktor ZBP-89 inhibira odgovor SOX3 promotora na indukciju retinoičnom kiselinom. Ova teza predstavlja prvu funkcionalnu karakterizaciju promotorskog regiona SOX3 gena čoveka, kao i identifikaciju transkripcionih faktora uključenih u regulaciju ekspresije ovog gena. Posebno treba istaći da rezultati prikazani u ovoj tezi ukazuju na kompleksnu, modularnu prirodu cis regulatornih elemenata odgovornih za transkripcionu regulaciju SOX3 gena." ]
[ "SOX3/Sox3 gene represents one of the earliest neural markers in vertebrates, playing a role in specifying neuronal fate. Despite the mounting evidence that SOX3 is the key player in early developmental gene regulation, little is known about the transcriptional regulation of the Sox3 gene itself. In this thesis, for the first time, we have performed analysis of the molecular mechanisms underlying the transcriptional regulation of SOX3 gene expression. Using primer extension, we have identified the transcription start point of this gene, 252 nt upstream of the ATG codone. Using promoter–reporter constructs, we have determined the minimal SOX3 promoter region (-219/+67) that confers the basal promoter activity in NT2/D1 cells. Deletion analysis of the minimal promoter revealed two cis regulatory regions (-219/-100 and +27/+67) that are necessary for SOX3 promoter activity. Also, in this study we have identified two regions, -427/-219 and +67/+286, that harbor positive cis regulatory elements necessary for both, optimal and retinoic acid (RA)-inducible promoter activity of the SOX3 gene. By additional functional analysis we have narrowed down the region required for RA induction of this gene to fragment -427/-293. Comparative analysis of the promoter sequences of SOX3 orthologues showed that binding sites for transcription factors Sp1, USF, NF-Y and CREB , as well as, TATA box are conserved in both position and sequence among all analysed mammalian orthologues. Data presented in this thesis, for the first time, suggest that transcription factors Sp1, USF1, NF-Y, CREB and MAZ function as transcriptional activators of the SOX3 gene expression. On the other hand, the presented results indicate that ZBP-89 transcription factor inhibits the response of SOX3 promoter upon retinoic acid induction. In this study, we have performed the first characterization of the human SOX3 promoter and identified transcription factors involved in regulation of its expression. Results presented in this thesis suggest that transcriptional regulation of the SOX3 gene depends on the combined action of distinct regulatory modules within the promoter region of this gene." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "" ]
Универзитет у Београду
[ "minimalni promotor", "SOX3", "Sp1", "USF1", "NF-Y", "CREB", "MAZ", "ZBP-89" ]
[ "minimal promoter", "SOX3", "Sp1", "USF1", "NF-Y", "CREB", "MAZ", "ZBP-89" ]
[ "Karakterizacija bazalnog promotora humanog SOX3 gena i identifikacija regulatornih elemenata odgovornih za indukciju SOX3 gena retinoičnom kiselinom" ]
[ "Characterization of the basal promoter of the human SOX3 gene and identification of the regulatory elements responsible for the SOX3 gene induction by retinoic acid" ]
doctoralThesis
[ "ARR" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1668/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1668/Disertacija.pdf", "https://fedorabg.bg.ac.rs/fedora/get/o:19450/bdef:Content/download" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10969" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/11606
123456789-11606.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:20537/bdef:Content/download
no
Procena antigenotoksičnog potencijala etanolnog ekstrakta lista masline (Olea europaea L.) u prisustvu hormona tiroksina, adrenalina, estradiola i dietilstilbestrola u leukocitima periferne krvi in vitro kod čoveka
Evaluation of antigenotoxic potential of ethanolic olive leaf extract (Olea europaea L.) on the effect of thyroxine, adrenaline, estradiol and diethylstilbestrol in human peripheral blood leukocytes in vitro
[ "Živković, Lada" ]
[ "Savić-Veselinović, Marija", "Đelić, Ninoslav", "Živković, Lada", "Savić-Veselinović, Marija" ]
Topalović, Dijana
2019-12-24T16:06:41
[ "2019-12-24T16:06:41", "2020-07-03T08:07:47" ]
2019-09-25
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=7002", "https://nardus.mpn.gov.rs/handle/123456789/11606", "https://fedorabg.bg.ac.rs/fedora/get/o:20537/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51737103" ]
[ "Hormoni su organska jedinjenja različite hemijske prirode koji svojim dejstvom utiču na rast, funkciju i metabolizam organizma. Povišene koncentracije hormona dovode do stvaranja slobodnih radikala, što može izazvati oksidativni stres i oštećenja DNK molekula. Poznato je da komponente tradicionalne mediteranske ishrane imaju pozitivne efekte na smanjenje oksidativnog stresa i prevenciju mnogih bolesti. Brojne in vitro i in vivo studije su potvrdile korisne efekte suvog ekstrakta lista masline (DOLE) i njegovih sastojaka, kao i zaštitni potencijal u odnosu na oksidativna oštećenja DNK molekula. Stoga, glavni cilj ove doktorske disertacije je bio da se ispita antigenotoksični potencijal spektra koncentracija suvog ekstrakta lista masline u leukocitima periferne krvi čoveka u prisustvu hormona tiroksina, adrenalina, estradiola i dietilstilbestrola in vitro. Ispitivanje je obavljeno primenom alkalnog komet testa, osetljive i brze metode za određivanje i analizu primarnih oštećenja DNK u pojedinačnim ćelijama. Rezultati ove studije su pokazali da su tiroksin, adrenalin, estradiol i dietilstibestrol sposobni da budu medijatori značajnog povećanja oštećenja DNK molekula. Suvi ekstrakt lista masline je u svim testiranim koncentracijama ispoljio značajan antigenotoksični potencijal u oba eksperimentalna protokola, u pretretmanu i posttretmanu. Praćenje kinetike reparacije DNK u prisustvu DOLE u odnosu na oštećenja izazvana hormonima je pokazalo da ekstrakt nije značajno uticao na stimulaciju reparacije oštećenja DNK. Sumirajući rezultate ove disertacije, može se zaključiti da suvi ekstrakt lista masline poseduje izražen potencijal smanjenja primarnih oštećenja DNK izazvanih tiroksinom, adrenalinom, estradiolom i dietilstilbestrolom. Predstavljeni in vitro model na leukocitima periferne krvi čoveka pružio je podatke koji su korisni za buduće in vivo studije i klinička ispitivanja suvog ekstrakta lista masline." ]
[ "Hormones are organic compounds of different chemical nature that affect cell growth, function and metabolism. Elevated concentrations of hormones lead to the formation of free radicals, which can cause oxidative stress and damage to the DNA molecule. It is known that the components of traditional Mediterranean food have positive effects on the reduction of oxidative stress and the prevention of many diseases. Numerous in vitro and in vivo studies have confirmed the beneficial effects of dry olive leaf extract (DOLE) and its constituents, as well as protective potential on the oxidative damage of DNA molecules. Therefore, the main aim of this doctoral dissertation was to investigate the antigenotoxic potential of the spectrum of concentrations of dry olive leaf extract in peripheral blood leukocytes in the presence of hormone thyroxine, adrenaline, estradiol, and diethylstilbestrol in vitro. The study was performed using an alkaline comet assay, a sensitive and fast method for determining and analyzing primary DNA damage in individual cells. The results of this study have shown that thyroxine, adrenaline, estradiol, and diethylstibestrol are capable of being mediators of significant increase of DNA damage. Dry olive leaf extract demonstrated significant ability to reduce primary DNA damage at all tested concentrations and in both experimental protocols, in pretreatment and posttreatment. Monitoring the kinetics of DNA repair in the presence of DOLE in relation to damage induced by homones has shown that the extract did not significantly affect the stimulation of DNA damage repair. Summarizing the results of this dissertation, it can be concluded that the dry olive leaf extract possesses a strong potential to reduce primary DNA damage induced by thyroxine, adrenaline, estradiol, and diethylstilbestrol. The presented in vitro model on human peripheral blood leukocytes provided data that are useful for future in vivo studies and clinical trials of dry olive leaf extract." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173034/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "suvi ekstrakt lista masline (DOLE)", "tiroksin", "adrenalin", "estradiol", "dietilstilbestrol", "antigenotoksičnost", "oštećenja DNK" ]
[ "dry olive leaf extract (DOLE)", "thyroxine", "adrenaline", "estradiol", "diethylstilbestrol", "antigenotoxicity", "DNA damage" ]
[ "Procena antigenotoksičnog potencijala etanolnog ekstrakta lista masline (Olea europaea L.) u prisustvu hormona tiroksina, adrenalina, estradiola i dietilstilbestrola u leukocitima periferne krvi in vitro kod čoveka" ]
[ "Evaluation of antigenotoxic potential of ethanolic olive leaf extract (Olea europaea L.) on the effect of thyroxine, adrenaline, estradiol and diethylstilbestrol in human peripheral blood leukocytes in vitro" ]
doctoralThesis
[ "BY-NC-ND" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1687/IzvestajKomisije21222.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1686/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1686/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1687/IzvestajKomisije21222.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_11606" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/2057
123456789-2057.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:3484/bdef:Content/download
no
Ekspresija gena za inhibitor serinskih proteinaza (BvSTI) šećerne repe (Beta vulgaris L.) i uloga u otpornosti na insekte
Expression of sugar beet (Beta vulgaris L.) serine proteinase inhibitor gene (BvSTI) and the role in insect resistance
[ "Ninković, Slavica" ]
[ "Radović, Svetlana", "Smigocki, Ann" ]
Savić, Jelena
2016-01-05T11:46:15
[ "2016-01-05T11:46:15", "2020-07-03T08:07:13" ]
2012-12-21
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=81", "https://nardus.mpn.gov.rs/handle/123456789/2057", "https://fedorabg.bg.ac.rs/fedora/get/o:3484/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=42099471" ]
[ "Biljni inhibitori proteinaza aktivno učestvuju u odbrani biljaka od insekata štetočina inhibirajući insekatske digestivne proteinaze. In planta analiza ekspresije BvSTI gena koji kodira inhibitora serinskih proteinaza urađena je sa ciljem otkrivanja uloge ovog inhibitora u otpornosti biljaka šećerne repe na insekte, kao i radi utvrđivanja potencijala ovog gena kao pogodnog kandidat-gena koji bi se biotehnološkim metodama mogao uvesti u osetljive biljne genome, čime bi se povećala njihova otpornost prema insektima štetočinama. Ekspresija BvSTI gena praćena je kod tri genotipa šećerne repe koji se odlikuju umerenom otpornošću prema larvama štetočine korena Tetanops myopaeformis Roder, F1016, F1015 i UT-8, kao i kod jednog osetljivog genotipa, F1010. Kod svih otpornih genotipova mehaničko povređivanje indukovalo je ekspresiju ovog gena, a nivo transkripcije bio je povišen u poređenju sa nivoom kod osetljivog genotipa. Najintenzivniji odgovor na povređivanje zabeležen je kod otpornih genotipova F1016 i UT-8. U listovima osetljivog F1010, ali i trećeg otpornog genotipa F1015, registrovan je samo neznatni porast u intenzitetu BvSTI transkripcije, dok je u korenovima ova dva genotipa mehaničko povređivanje dovelo do blage početne supresije u aktivnosti BvSTI gena. Akumulacija BvSTI transkripata u listovima i korenovima otpornog F1016 i osetljivog F1010 kojima su se hranile larve Spodoptera frugiperda J.E. Smith pokazala je sličan obrazac ekspresije kod oba genotipa. U poređenju sa transkripcionim obrascima dobijenim nakon mehanilčkog povređivanja, ishrana insekata dovela je do znatno sporije indukcije i slabijeg intenziteta transkripcije. Analize na proteinskom nivou pokazale su da nakon povređivanja listova dolazi do akumulacije proteina veličine 30 kDa za koji su se vezala poliklonalna BvSTI specifična antitela kako kod otpornog F1016, tako i kod osetljivog F1010 genotipa. Aktivnost BvSTI inhibitora protiv tripsina pokazana je kod F1016 korenova i listova, kao i kod F1010 listova. U F1010 korenovima aktivnost BvSTI inhibitora nije detektovana. U biotestu u kome je ispitivana otpornost pojedinačnih genotipova na larve S. frugiperda korišćena su sva četiri genotipa šećerne repe kod kojih je pokazano da povređivanje utiče na ekspresiju BvSTI gena. Larve koje su se hranile listovima sva tri otporna genotipa bile su statistički značajno lakše od larvi koje su hranile osetljivim F1010 listovima..." ]
[ "Plant proteinase inhibitor genes are among the prime candidates suitable for insect resistance improvement in plants. Expression pattern of a sugar beet serine proteinase inhibitor gene, BvSTI, was characterized in response to mechanical and fall armyworm (Spodoptera frugiperda J.E. Smith) induced wounding. BvSTI expression was analyzed in three breeding lines moderately resistant to sugar beet root maggot (Tetanops myopaeformis Roder.) and in a susceptible line, F1010. Increased mechanical wounding induced levels of BvSTI expression were observed in all resistant lines as compared to F1010. The most intensive response to wounding was observed in resistant lines, F1016 and UT-8, with a maximum up to 4- and 2,5-fold increase of BvSTI transcript levels over non-wounded roots and leaves, respectively. In contrast, slight increase of BvSTI transcript levels in leaves and even an initial decrease in roots were observed in sensitive F1010, but also in the third resistant line, F1015. BvSTI transcript accumulation in F1016 and F1010 tissues wounded by FAW showed a similar gene expression pattern, but it was delayed and less intense than the response incited by abiotic wounding. On the protein level, BvSTI-specific polyclonal antibodies confirmed increased accumulation of the 30 kDa BvSTI protein in wounded leaves but not in roots of F1016 and F1010. Using trypsin inhibition assays, the activity of BvSTI was confirmed in F1016 roots and leaves and F1010 leaves. In F1010 roots BvSTI activity was completely lacking. To confirm the potential role of the BvSTI gene in defending mechanisms to insect pests in sugar beet the same analyzed germplasm were bioassayed for resistance to fall armyworm insects. Larvae fed sugar beet leaves from all three resistant germplasms (F1016, F1015 and UT-8) had significant reductions in larval weights as compared to larvae fed on sensitive F1010 leaves. The observed daily weight increase was also the highest in larvae from sensitive vs. resistant leaves. As the larvae entered the pupal stage, pupal sizes did not reflect the overall larval weights and all developed pupae were similar. Larvae fed on roots were almost double lighter than larvae from leaves for all analyzed gemplasms. Some developmental abnormalities of the pupae fed on F1016 and F1015 leaves were noted..." ]
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Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31049/RS//", "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173015/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "otpornost prema insektima", "Inhibitor serinskih proteinaza", "BvSTI gen", "Šećerna repa", "Inducibilni genski promotori", "transgeni duvan", "Spodoptera frugiperda" ]
[ "insect resistance", "Serine proteinase inhibitor", "BvSTI gene", "Sugar beet", "Inducible gene promoters", "transgenic tobacco", "Spodoptera frugiperda" ]
[ "Ekspresija gena za inhibitor serinskih proteinaza (BvSTI) šećerne repe (Beta vulgaris L.) i uloga u otpornosti na insekte", "Expression of sugar beet (Beta vulgaris L.) serine proteinase inhibitor gene (BvSTI) and the role in insect resistance" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Нинковић, Славица; Смигоцки, Aнн; Радовић, Светлана; Савић, Јелена; Експресија гена за инхибитор серинских протеиназа (БвСТИ) шећерне репе (Бета вулгарис Л.) и улога у отпорности на инсекте; Експресија гена за инхибитор серинских протеиназа (БвСТИ) шећерне репе (Бета вулгарис Л.) и улога у отпорности на инсекте;
[ "http://nardus.mpn.gov.rs/bitstream/id/1551/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1551/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2057" ]
10.2298/bg20121221savic
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/2089
123456789-2089.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:3502/bdef:Content/download
no
Uticaj trombina na regulaciju proliferacije glatkih mišićnih ćelija aorte pacova
Effects of trombin in the regulation of rat's aortic smooth muscle cells proliferation
[ "Isenović, Esma" ]
[ "Isenović, Esma", "Đorđević, Jelena", "Đorđević, Jelena", "Putniković, Biljana" ]
Smiljanić, Katarina
2016-01-05T11:46:45
[ "2016-01-05T11:46:45", "2020-07-03T08:09:27" ]
2012-10-22
[ "https://nardus.mpn.gov.rs/handle/123456789/2089", "http://eteze.bg.ac.rs/application/showtheses?thesesId=92", "https://fedorabg.bg.ac.rs/fedora/get/o:3502/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024530866" ]
[ "Kardiovaskularne bolesti predstavljaju najveći uzrok smrtnosti ljudske populacije, a njihova glavna i osnovna patološka komponenta je ateroskleroza. Proliferacija ili deoba glatkih mišićnih ćelija krvnog suda (VSMC) ključni je događaj u nastanku raznih vaskularnih oboljenja, uključujući aterosklerozu i hipertenziju. U procesu diferencijacije i abnormalne deobe VSMC povezanih sa hipertenzijom i aterosklerozom uključen je i trombin. Stimulisanje VSMC trombinom dovodi do aktivacije ekstracelularnim signalima regulisanih kinaza 1 i 2 (ERK1/2), preko transaktivacije receptora za epidermalni faktor rasta (EGFR). U ranijim studijama Isenović i saradnici potvrdili su na osnovu inhibicije ERK1/2 od strane PD9805 inhibitora, učešće ERK1/2 u regulaciji proliferacije VSMC izazvanoj trombinom. U nastavku, faktor rasta sličan epidermalnom faktoru rasta koji vezuje heparin (HB-EGF), protein kinaza C delta (PKCδ) i matriksne metaloproteinaze (MMP), nađene su u VSMC i pokazano je da je i njihova aktivnost regulisana trombinom. ADAM (engl. “A Disintegrin And Metalloproteinase”) transmembranske su metaloproteinaze koje pripadaju adamalizinskoj grupi i razlikuju se od matriksnih metaloproteinaza po tome što imaju vanćelijski dizintegrinski i citoplazmatski domen. C terminalni domen može da stupa u interakciju sa unutarćelijskim proteinima. Uvidom u literaturu, do sada ne postoji istraživanje koje ukazuje na prisustvo ADAM metaloproteinazne aktivnosti vezane za proliferaciju VSMC stimulisane trombinom. Predmet ove doktorske teze jeste proučavanje uloge EGFR, ERK1/2, HB-EGF, PKCδ, ukupnih metaloproteinaza, kao i specifičnih MMP-2 i ADAM 12, u posredovanju proliferativnog efekta trombina na VSMC pacova. Inkubacija VSMC pacova sa trombinom (1 U/ml) u periodu od 5 minuta rezultirala je u značajnom povećanju: fosforilacije ERK1/2 od 8.7 ± 0.9 puta (p<0.001), fosforilacije EGFR od 8.5 ± 1.3 puta (p<0.001) i sinteze DNK od 3.6 ± 0.4 puta (p<0.001). Prethodni pojedinačni tretmani ovih ćelija u trajanju od 30 minuta sa 10 μM PD169540 (PD), ireverzibilnim inhibitorom EGFR, i 20 μM antitelom protiv HB-EGF značajno su smanjili trombinom stimulisanu fosforilaciju EGFR za 81 % i 72 % i ERK1/2..." ]
[ "ardiovascular disease is the largest single cause of mortality and its major underlying pathology is atherosclerosis. The proliferation of vascular smooth muscle cells (VSMC) is a key event in the pathogenesis of various vascular diseases, including atherosclerosis and hypertension. Thrombin is involved in the differentiation and abnormal proliferation of VSMC associated with atherosclerosis and hypertension. Thrombin stimulation results in extracellular signal-regulated kinase (ERK 1/2) activation through transactivation of the epidermal growth factor receptor (EGFR). Based on the studies in which PD98059 used to inhibit ERK1/2, we have shown previously that ERK1/2 was involved in the regulation by thrombin of VSMC’s proliferation. In addition, heparin-binding EGF-like growth factor (HB-EGF) and matrix metalloproteinases (MMPs) have also been detected in VSMC and shown to be regulated by thrombin. ADAMs (A Disintegrin And Metalloproteinase) are transmembrane metalloproteinases, belonging to adamalysin group, that are distinct from matrix metalloproteinases (MMPs) in that, they have an extracellular disintegrin domain and cytoplasmic domain that can associate with intracellular proteins. To the present knowledge there is no study that indicates the activation of an ADAM member in thrombin-induced VSMC proliferation. In this dissertation, the role of EGFR, ERK1/2, HB-EGF, general metalloproteinases, MMP-2 and ADAM 12, as well as PKCδ in mediating the mitogenic action of Thrombin in rat VSMC was investigated. Incubation of rat VSMC with Thrombin (1 U/ml) for 5 minutes resulted in significant increase of ERK1/2 phosphorylation by 8.7 ± 0.9 fold (p<0.001), EGFR phosphorylation by 8.5 ± 1.3 fold (p<0.001) and DNA synthesis by 3.6 ± 0.4 fold (p<0.001). Separate pretreatments for 30 minutes with EGFR tyrosine kinase irreversible inhibitor, 10 μM PD169540 (PD), and 20 μM anti-HB-EGF antibody, significantly reduced thrombinstimulated EGFR and ERK1/2 phosphorylation by 81 %, 72 % and by 48 % and 61%, respectively. Furthermore, same pretreatments with PD and anti-HB-EGF antibody reduced Thrombin-induced VSMC’s proliferation by 44% and 45%, respectively. In addition, pretreatments for 30 minutes with 10 μM KB-R7785 (KB), a specific ADAM 12 inhibitor or 10 μM specific MMP2 inhibitor significantly reduced thrombin-stimulated EGFR.." ]
application/pdf
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Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by/4.0/" ]
Универзитет у Београду
[ "trombin", "VSMC", "proliferacija", "ADAM 12", "MMP-2", "PKCδ", "ERK1/2", "HB-EGF", "ateroskleroza" ]
[ "Thrombin", "VSMC", "Proliferation", "ADAM 12", "MMP-2", "PKCδ", "EGFR", "ERK1/2", "HB-EGF", "Aterosclerosis" ]
[ "Uticaj trombina na regulaciju proliferacije glatkih mišićnih ćelija aorte pacova", "Effects of trombin in the regulation of rat's aortic smooth muscle cells proliferation" ]
[ "" ]
doctoralThesis
[ "BY" ]
Исеновић, Есма; Ђорђевић, Јелена; Путниковић, Биљана; Ђорђевић, Јелена; Исеновић, Есма; Смиљанић, Катарина; Утицај тромбина на регулацију пролиферације глатких мишићних ћелија аорте пацова; Утицај тромбина на регулацију пролиферације глатких мишићних ћелија аорте пацова;
[ "https://nardus.mpn.gov.rs/bitstream/id/2097/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2097/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2089" ]
10.2298/bg20121022smiljanic
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/11504
123456789-11504.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:20260/bdef:Content/download
no
Анализа експресије eNOS и ангиогених фактора HIF-1alfa и VEGF у мијелопролиферативним неоплазмама: веза са присуством мутација у генима за JAK2 i CALR
Analysis of the expression of eNOS and angiogenic factors HIF-1alfa and VEGF in myeloproliferative neoplasms: a connection with the presence of mutations in the genes for JAK2 and CALR
[ "Mitrović-Ajtić, Olivera" ]
[ "Čakić-Milošević, Maja", "Čokić, Vladan" ]
Суботички, Тијана
2019-11-15T10:03:54
[ "2019-11-15T10:03:54", "2020-07-03T08:07:55" ]
2019-04-12
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6911", "https://nardus.mpn.gov.rs/handle/123456789/11504", "https://fedorabg.bg.ac.rs/fedora/get/o:20260/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025217202" ]
[ "Мијелопролиферативне неоплазме (МПН) представљају клоналне болести матичних ћелија хематопоезе које карактерише поремећај пролиферације једне или више мијелоидних ћелијских линија. МПН обухватају 3 ентитета: полицитемију веру (ПВ), есенцијалну тромбоцитемију (ЕТ) и примарну мијелофиброзу (ПМФ). Најзаступљенији поремећај код МПН је JAK2V617F мутација која је присутна код 97% пацијената са дијагнозом ПВ и 50-60% пацијената са дијагнозама ЕТ и ПМФ. JAK2V617F мутација индукује конститутивну активацију нисходних сигналних путева (JAK2/STAT3, PI3K/АКТ, MAPK) који су укључени у мијелопролиферацију. Друга најзаступљенија соматска мутација, која је присутна код већине JAK2V617F негативних ЕТ и ПМФ пацијената, повезана је са геном који кодира калретикулин (CALR) присутна код 31,9% ЕТ и 31,7% ПМФ пацијената. Код различитих хематолошких малигнитета, укључујући и МПН, као одраз појачане ангиогенезе, описано је присуство повишене експресије фактора раста васкуларног ендотела (VEGF). У новијим истраживањима показана је веза између експресије VEGF гена и нивоа хипоксија-индуцибилног фактора-1 (HIF-1) азот моноксида (NО). Неколико студија до сада је показало да NО може индуковати продукцију VEGF путем PI3K/Akt/PKB/HIF-1α пута, али и да делује као инхибитор HIF-1α експресије. Циљ истраживања ове дисертације било је испитивање степена експресије и корелација кључних ангиогених фактора - HIF-1α, VEGF и ендотелне NО синтазе (eNOS) у костној сржи, CD34+ ћелијама и гранулоцитима МПН пацијената према JAK2 и CALR статусу и терапији. Поред тога, користили смо проинфламаторни IL-6 да индукујемо експресију HIF-1α, VEGF и eNOS у хуманој HEL 92.1.7 ћелијској линији са JAK2 мутацијом, као и у диферентованим макрофагама. Такође испитивано је учешће различитих сигналних путева (ЈАК2/STAT3, PI3К/Аkt, MAPK) путем којих IL-6 остварује своје ефекте, на моделу HEL ћелијске линије као и код гранулоцита МПН пацијената. Резултати ове студије су показали да ангиогени фактори имају израженију експресију у гранулоцитима него у CD34+ и ћелијама костне сржи, што указује на значај инфламаторних гранулоцита у развоју ангиогенезе. Показана је и значајна негативна корелација између протеинске експресије HIF-1α и VEGF, као и HIF-1α и еNOS, док између VEGF и eNOS постоји значајна позитивна корелација код гранулоцита МПН пацијената. Стандардно лечење применом хидроксиуреје код МПН пацијената има антиангиогени потенцијал у смислу смањења сва три ангиогена фактора – HIF-1α, VEGF и eNOS у гранулоцитима..." ]
[ "hematopoiesis characterized by a disorder of proliferation of one or more myeloid cell lines. MPNs include 3 entities: polycythemia (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF). The most common disorder in MPN is the JAK2V617F mutation present in 97% of patients diagnosed with PV and 50-60% of patients with ET and PMF diagnoses. The JAK2V617F mutation induces the constituent activation of the innate signal pathways (JAK2 / STAT3, PI3K / AKT, MAPK) involved in myeloproliferation. The second most common somatic mutation, which is present in most JAK2V617F negative ET and PMF patients, is associated with the calreteculin (CALR) encoding genome present in 31.9% ET and 31.7% of PMF patients. In the case of various hematological malignancies, including MPN, as a reflection of increased angiogenesis, the presence of elevated expression of vascular endothelial growth factor (VEGF) has been described. Recent studies have shown the relationship between the expression of the VEGF gene and the levels of hypoxia-inducible factor-1 (HIF-1) and nitrogen monoxide (NO). Several studies have so far shown that NO can induce the production of VEGF via PI3K / Akt / PKB / HIF-1α times, but also acts as an inhibitor of HIF-1α expression. The aim of the study was to investigate the degree of expression and correlation of key angiogenic factors - HIF-1α, VEGF and endothelial NO synthase (eNOS) in the bone marrow, CD34+ cells and granulocytes of MPN patients according to JAK2 and CALR status and therapy. In addition, we used proinflammatory IL-6 to induce the expression of HIF-1α, VEGF and eNOS in the human HEL 92.1.7 cell line with a JAK2 mutation, as well as in differentiated macrophages. Also studied was the participation of various signaling pathways (JAK2 / STAT3, PI3K / Akt, MAPK) through which IL-6 achieved its effects on the HEL cell line model as well as in granulocyte of MPN patients. The results of this study have shown that angiogenic factors have a more pronounced expression in granulocytes than in CD34+ and bone marrow cells, which indicates the importance of inflammatory granulocytes in the development of angiogenesis. A significant negative correlation between HIF-1α and VEGF protein expression, as well as HIF-1α and eNOS, was shown, while there was a significant positive correlation between VEGF and eNOS in granulocytes of MPN patients. Standard treatment with hydroxyurea in MPN patients has antiangiogenic potential in terms of reducing all three angiogenic factors - HIF-1α, VEGF and eNOS in granulocytes..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175053/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "мијелопролиферативне неоплазме", "ангиогенеза", "HIF-1α", "VEGF", "eNOS", "IL-6", "STAT3", "Akt", "MAPK" ]
[ "myeloproliferative neoplasm", "angiogenesis", "HIF-1α", "VEGF", "eNOS", "IL-6", "STAT3", "Akt", "MAPK" ]
[ "Анализа експресије eNOS и ангиогених фактора HIF-1alfa и VEGF у мијелопролиферативним неоплазмама: веза са присуством мутација у генима за JAK2 i CALR" ]
[ "Analysis of the expression of eNOS and angiogenic factors HIF-1alfa and VEGF in myeloproliferative neoplasms: a connection with the presence of mutations in the genes for JAK2 and CALR" ]
doctoralThesis
[ "BY-NC-ND" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1732/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1733/IzvestajKomisije21106.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1733/IzvestajKomisije21106.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1732/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_11504" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/10256
123456789-10256.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:18933/bdef:Content/download
no
Rasprostranjenje, ekologija i predviđanje distribucije invazivnih taksona roda Reynoutria Houtt. (Polygonaceae) na području Srbije i jugoistočne Evrope
Distribution, ecology and prediction of potential ranges of invasive Reynoutria Houtt. taxa (Polygonaceae) in Serbia and southeastern Europe
[ "Jovanović, Slobodan" ]
[ "Lakušić, Dmitar", "Šinžar-Sekulić, Jasmina", "Anačkov, Goran" ]
Hlavati-Širka, Vesna M.
2018-12-10T11:45:11
[ "2018-12-10T11:45:11", "2020-07-03T08:06:55" ]
2018-09-22
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6286", "https://nardus.mpn.gov.rs/handle/123456789/10256", "https://fedorabg.bg.ac.rs/fedora/get/o:18933/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50782735" ]
[ "Iako su invazivni predstavnici roda Reynoutria proučavani od strane više autora, prevashodno na nivou pojedinačnih država, pojedinačnih staništa ili zaštićenih područja, nema naučnih radova u kojima se celovito analizira njihova ekologija, ukupno rasprostranjenje, kao i predviđanje buduće distribucije na području Srbije i jugoistočne Evrope. Takođe, evidentna je nedovoljna istraženost mehanizama adaptacije i strategije preživljavanja ovih taksona u antropogeno zagađenim staništima. Zbog svega, osnovni ciljevi ove disertacije su utvrđivanje prisustva i distribucije invazivnih taksona roda Reynoutria na području Srbije i jugoistočne Evrope, ekološka analiza u odnosu na klimatske parametre i tip staništa, procena invazibilnosti zaštićenih područja Srbije i jugoistočne Evrope, kao i analiza bioakumulacionih potencijala za makronutrijente i teške metale taksona R. x bohemica. Istraživanja su obavljena u periodu 2006. – 2016. godina. Zabeleženo je ukupno 4081 lokaliteta, u okviru tri različita tipa staništa. Najčešći taksoni su R. japonica i hibrid R. × bohemica. Primenom metode MaxEnt modelovanja utvrđeno je da padavine najtoplijeg kvartala (BIO18) imaju najveći uticaj na formiranje ekološke niše ovih taksona. Modelovana niša taksona R. × bohemica ukazuje na njegovu toleranciju visokih temperatura u različitim periodima godine, uz istovremeno niže količine padavina (i do 60 mm u odnosu na roditeljske vrste). Hemijskom analizom biljnih tkiva R.× bohemica, utvrđena je slaba akumulacija teških metala sa strategijom njihovog isključivanja, uz veoma efikasnu akumulaciju makronutrijenata. Rezultati upućuju na potrebu sprečavanja osnivanja novih populacija u blizini zaštićenih područja koja imaju pogodne sredinske faktore za razvoj ovih taksona." ]
[ "Although the invasive Reynoutria taxa have been studied by several authors, primarily at country, habitat or protected areas level, there are no scientific papers in which their ecology, distribution and future distribution predictions in Serbia and Southeastern Europe are completely analyzed. Also, there is lack of scientific research of adaptation mechanisms and survival strategies of these taxa in anthropogenically polluted soils. Hence, the main objectives of this dissertation are determination the presence and distribution of invasive Reynoutria taxa in Serbia and southeastern Europe, ecological study of bioclimatic parameters in different habitats and protected areas invasibility assessment in Serbia and Southeastern Europe, as well as R. × bohemica‘s bioaccumulation potential of macronutrients and heavy metals. Research was carried out in the period 2006-2016. A total of 4081 localities were recorded in seven SE Europe countries, in three types of habitats. The most frequent taxa are R. japonica and hybrid R. × bohemica. The precipitation of the warmest quarter (BIO18) had a highest influence on the potential distribution modelling of these taxons according to the MaxEnt. Potential distribution modelling revealed R. × bohemica's high temperature tolerance in different seasons with high durability of drought (up to 60 mm per year less precipitation than parental species). Weak heavy metal accumulation accompanied by exclusion, with very efficient macronutrients accumulation, seems to be the survival and expansion strategy of Reynoutria × bohemica according to the chemical analysis results. The results indicate the need to prevent the establishment of new populations near protected areas with suitable bioclimatic parameters for their development." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "strane vrste", "invazivne neofite", "modelovanje ekoloških niša", "potencijalna distribucija", "MaxEnt model", "analiza najkraćeg puta", "zaštićena prirodna dobra", "jugoistočna Evropa", "akumulacija teških metala" ]
[ "invasive alien species", "ecological niche modelling", "potential distribution", "MaxEnt model", "least-cost path analysis", "protected areas", "accumulation of heavy metals" ]
[ "Rasprostranjenje, ekologija i predviđanje distribucije invazivnih taksona roda Reynoutria Houtt. (Polygonaceae) na području Srbije i jugoistočne Evrope" ]
[ "Distribution, ecology and prediction of potential ranges of invasive Reynoutria Houtt. taxa (Polygonaceae) in Serbia and southeastern Europe" ]
doctoralThesis
[ "BY-NC-ND" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1476/IzvestajKomisije18470.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1475/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1475/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1476/IzvestajKomisije18470.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10256" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/10052
123456789-10052.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:18509/bdef:Content/download
no
Diverzitet aerofitskih cijanobakterija i algi u biofilmu odabranih pećina u Srbiji
Diversity of aerophytic cyanobacteria and algae in biofilm from selected caves in Serbia
[ "Subakov Simić, Gordana" ]
[ "Krizmanić, Jelena", "Krunić, Olivera" ]
Popović, Slađana S.
2018-11-07T15:53:13
[ "2018-11-07T15:53:13", "2020-07-03T08:07:03" ]
2018-07-06
[ "https://nardus.mpn.gov.rs/handle/123456789/10052", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6094", "https://fedorabg.bg.ac.rs/fedora/get/o:18509/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50645007" ]
[ "Predmet ove doktorste disertacije je analiza zajednice aerofitskih cijanobakterija i algi iz biofilma sa stenovitog supstrata ulaza petnaest odabranih pećina iz Srbije kao i nekoliko tačaka u blizini veštačkog osvetljenja. Akcenat istraživanja bio je na fototrofnim mikroorganizmima iz biofilma, dok su kao dodatak endolitski predstavnici identifikovani iz nekoliko uzoraka stene. Analizirana je i morfologija, stepen razvijenosti biofilma, kao i udeo sadržaja vode, organske i neorganske materije u biofilmu. U svim pećinama, mereni su ekološki parametri i određene su primarna produkcija (preko koncentracije hlorofila a) i petrografska analiza stenovitog supstrata. Na odabranim uzorcima rađena je kvantitativna i sezonska analiza cijanobakterija i algi. Statističke analize su poslužile kao alat za sagledavanje odnosa zabeleženih fototrofnih mikroorganizama i različitih ekoloških i opisnih parametara. Zabeleženo je prisustvo ukupno 290 taksona cijanobakterija i algi koje su svrstane u 4 razdela: Cyanobacteria (134 taksona, od kojih 82 taksona pripada kokoidnim formama), Bacillariophyta (129 taksona), Chlorophyta (26 taksona) i Xanthophyta (1 takson). Najveći broj taksona Cyanobacteria zabeležen je u rodovima Gloeocapsa, Chroococcus, Gloeothece, Leptolyngbya i Scytonema. U razdelu Bacillariophyta se izdvajaju rodovi Luticola i Humidophila, a u razdelu Chlorophyta rod Cosmarium. Iz biofilma Božanine pećine opisana je nova vrsta cijanobakterije za nauku, Nephrococcus serbicus S. Popović, G. Subakov Simić i J. Komárek, sp. nov. pri čemu je sam rod po prvi put zabeležen na teritoriji Evrope. Cyanobacteria po broju taksona dominiraju na svim lokalitetima, osim Rćanske i Bogovinske pećine. Najveći broj pripadnika Bacillariophyta i Chlorophyta zabeležen je u Rćanskoj (usled prisustva vode na biofilmu) i pećini Samar (zbog sezonskog uzorkovanja). Upravo se značaj sezonskog uzorkovanja biofilma u pećinama Samar i Jezava pre svega ogleda kroz znatno veći diverzitet aerofitskih cijanobakterija i algi u odnosu na druge pećine. Predstavnici Cyanobacteria dominirali su u pećinama čiji je supstrat rekristalisali i organogeni krečnjak, a Bacillariophyta na klastičnom i mikritskom krečnjaku..." ]
[ "The aim of this PhD thesis is the analysis of the aerophytic cyanobacterial and algal community from biofilm developed on stone substrate at the entrance of fifteen selected caves from Serbia and several sites near the artificial light in caves. The focus of the study was on phototrophic microorganisms from the biofilm, while endolithic representatives were additionaly identified from several stone samples. Morphology, degree of biofilm development, as well as the content of water, organic and inorganic matter in biofilm were also observed. At all sampling sites, ecological parameters, primary production (through concentration of chlorophyll a), as well as petrographic analysis of rocky substrates were measured and determined. Quantitative and seasonal analyzes of cyanobacteria and algae were assessed in selected samples. Statistical analyzes have served as a tool for examining the relationship between recorded phototrophic microorganisms and various ecological and descriptive parameters. A total of 290 cyanobacterial and algal taxa were recorded and classified into 4 divisions: Cyanobacteria (134 taxa, of which 82 taxa belong to the coccoid forms), Bacillariophyta (129 taxa), Chlorophyta (26 taxa) and Xanthophyta (1 taxon). The highest number of cyanobacterial taxa was documented within the following genera: Gloeocapsa, Chroococcus, Gloeothece, Leptolyngbya and Scytonema. Luticola and Humidophila were the most diverse genera within Bacillariophyta division, while in Chlorophyta Cosmarium had the highest number of species. A new cyanobacterial taxon, Nephrococcus serbicus S. Popović, G. Subakov Simić & J. Komárek, sp. nov., was described from a biofilm sample of Božana Cave. This also represents the first record of the genus Nephrococcus in Europe. Cyanobacteria were dominant in all caves, except Rćanska and Bogovinska cave. The largest number of Bacillariophyta and Chlorophyta was recorded in the Rćanska cave (due to the presence of water on samplins sites) and in the Samar Cave (due to seasonal sampling). The significance of the seasonal biofilm sampling in Samar and Jezava caves is primarily reflected through the higher diversity of aerophytic cyanobacteria and algae in comparison to other caves..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/176018/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "pećine", "biofilm", "fototrofni mikroorganizmi", "aerofitske cijanobakterije", "aerofitske alge", "Nephrococcus serbicus", "biomasa", "stenoviti supstrat", "krečnjak" ]
[ "caves", "biofilm", "phototrophic microorganisms", "aerophytic Cyanobacteria", "aerophytic algae", "Nephrococcus serbicus", "biomass", "stone substrata", "limestone" ]
[ "Diverzitet aerofitskih cijanobakterija i algi u biofilmu odabranih pećina u Srbiji" ]
[ "Diversity of aerophytic cyanobacteria and algae in biofilm from selected caves in Serbia" ]
doctoralThesis
[ "BY-NC-ND" ]
Субаков Симић, Гордана; Кризманић, Јелена; Крунић, Оливера; Поповић, Слађана С.; Диверзитет аерофитских цијанобактерија и алги у биофилму одабраних пећина у Србији; Диверзитет аерофитских цијанобактерија и алги у биофилму одабраних пећина у Србији;
[ "https://nardus.mpn.gov.rs/bitstream/id/1514/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1515/IzvestajKomisije17866.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1514/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1515/IzvestajKomisije17866.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10052" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/10261
123456789-10261.pdf
yes
yes
https://fedorabg.bg.ac.rs/fedora/get/o:18975/bdef:Content/download
no
Promene strukture DNK izazvane proteinom HsOrc4
Structural changes of DNA mediated by HsOrc4 protein
[ "Stefanović, Dragana" ]
[ "Radović, Svetlana", "Matić, Gordana" ]
Divac, Aleksandra
2018-12-10T11:45:17
[ "2018-12-10T11:45:17", "2020-07-03T08:06:45" ]
2008-04-23
[ "https://nardus.mpn.gov.rs/handle/123456789/10261", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6314", "https://fedorabg.bg.ac.rs/fedora/get/o:18975/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=33875983" ]
[ "У ћелијама свих живих организама репликација ДНК се одвија у неколико сложених и међусобно веома сличних фаза, а започиње тек када иницијаторни протеински комплекс препозна специфичне секвенце у геному. И поред великог биолошког значаја процеса репликације мало се зна о механизмима који регулишу иницицијацију синтезе ДНК вишећелијских еукариота. Ори секвенце ових организама не садрже консензус секвенце, али су богате АТ базним паровима груписаним у кратке, наизменичне или хомогене низове. Овакви елементи могу да формирају алтернативне структуре, а оне могу бити значајне за интеракцију ори секвенци и иницијаторног комплекса, или могу настати током интеракције иницијационог комплекса и ДНК и бити значајне за наредне фазе иницијације. У овом раду су испитиване интеракције рекомбинантног протеина HsOrc4 са фрагментом изолованим из хуманог репликатора LMNB2 и различитим синтетским ДНК које саджре А и Т елементе. Протеин је изабран за анализу јер чини део комплекса ORC (eng. Origin Recognition Complex), показује самосталну везивну активност и припада фамилији ААА+ суперфамилије макромолекулских шаперона. У раду је потврђено да се HsOrc4 преференцијално везује за ДНК богату АТ базним паровима и ТАТ елементима, и показано да стимулише некакнонску ренатурацију комплементарних фрагмената у специфичну троланчану структуру. Такође је показано да овај протеин стимулише и формирање ТАТ триплекса од одговарајућих олигонуклеотида. У раду је описана и сасвим неочекивана способност протеина да катализује асоцијацију хомоаденинских олигонуклеотида у раније непознате дволанчане и, вероватно, четвороланчане структуре. Хомоаденинске структуре биле су зависне од магнезијумових јона, термостабилне и повезане Хугстиновим везама. Судећи према деловању мутираних форми протеинa HsOrc4 неактивних у везивању или хидролизи АТР-а, за каталитичку активност HsOrc4 неопходно је везивање, али не и хидролиза АТР-а. Будући да је основна улога иницијационог комплекса да ремоделује ори секвенцу и припреми је за наредне фазе иницијације, описано деловање протеина HsOrc4 могло би да допринесе формирању комплексне архитектуре ори репликације и да има значајну улогу у иницијацији репликације." ]
[ "In multicellular eukaryotes DNA replication initiates at fixed chromosomal sites called origins of replication (ori). They host initiation start sites and binding sites for universal eukaryotic initiator called origin recognition complex (ORC), but do not share conserved consensus sequences. All origins are AT rich and contain homopurine/homopyrimidine stretches that can form alternative DNA structures, potentially important for origin function. In order to understand alternative structures of origins and the manner in which specific proteins influence DNA structure, we have investigated interactions of recombinant protein HsOrc4 with various origin or synthetic DNAs. HsOrc4 was chosen as DNA-binding component of ORC and a member of the AAA+ superfamily of macromolecular chaperones. HsOrc4 preferentially recognized double-stranded and triple-stranded AT rich DNA and stimulated renaturation of the origin fragment lboI into specific triple stranded structure, or renaturation of complementary oligonucleotides into intermolecular TAT triplex. An unexpected feature of HsOrc4 shown in our study was its ability to catalyse self-association of adenines creating homoadenine duplexes and possibly quadruplexes. The action of HsOrc4 was dependant on the presence of magnesium ions and ATP. ATP had a role as a cofactor, because HsOrc4 did not require its hydrolysis for activity. The mutated form HsOrc4 WA, which could not bind ATP, was inactive in restructuring reactions, whereas mutated form HsOrc4 WB, unable to hydrolyse ATP, showed similar activity to the wild type HsOrc4. Ability to form Hoogsteen bonds was essential for substrates in restructuring reactions, as these bonds held newly formed structures together. Substrates containing 7-Deaza-ATP, dATP analogue unable to form Hoogsteen bonds, were inactive in these assays. The features of HsOrc4 shown in this study are potentially biologically important, because its action could expand structural repertoire of genomes by producing energetically unfavourable DNA structures. These structures may be involved in determination of origin specificity." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "" ]
Универзитет у Београду
[ "Структура ДНК", "ДНК триплекс", "исходиште (ори) репликације", "ORC (origin recognition) комплекс", "протеин HsOrc4" ]
[ "DNA structure", "DNA triplex", "origin of replication", "ORC complex", "HsOrc4 protein" ]
[ "Promene strukture DNK izazvane proteinom HsOrc4" ]
[ "Structural changes of DNA mediated by HsOrc4 protein" ]
doctoralThesis
[ "ARR" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1444/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1444/Disertacija.pdf", "https://fedorabg.bg.ac.rs/fedora/get/o:18975/bdef:Content/download" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10261" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/2165
123456789-2165.pdf
no
no
https://fedorabg.bg.ac.rs/fedora/get/o:7999/bdef:Content/download
no
Састав, анимикробна и антиоксидативна активност етарских уља Thymus algeriensis, Th. capitatus, Salvia fruticosa и Satureja thymbra из Либије
Composition, antimicrobial and antioxidant activity of the essential oils of Thumus algeriensis, Thymus capitatus, Satureja thymbra and Salvia fruticosa from Libya
[ "Marin, Petar" ]
[ "Džamić, Ana", "Soković, Marina", "Janaćković, Peđa" ]
Giweli, Abdulhmid Ahmed
2016-01-05T11:48:30
[ "2016-01-05T11:48:30", "2020-07-03T08:07:25" ]
2013-11-16
[ "https://nardus.mpn.gov.rs/handle/123456789/2165", "http://eteze.bg.ac.rs/application/showtheses?thesesId=1160", "https://fedorabg.bg.ac.rs/fedora/get/o:7999/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=44645647" ]
[ "У овом раду анализиран је састав етарских уља неких биљних врста из фамилије Lamiaceae које расту kao самониклe у Либији (Thymus capitatus, Th. algeriensis, Satureja thymbra и Salvia fruticosa) коришћењем GC и GC-MS техника. Уља Thymus capitatus, Th. algeriensis и Salvia fruticosa карактеришу се присуством оксигенованих монотерепена, који су заступљени са 87.60%, 54.67% и 64.89%. Монотерпенски угљоводоници су главна група једињења код врсте Satureja thymbra , чинећи 58.57% од укупног уља. Главна једињења етарског уља Thymus capitatus су карвакрол (68.19%) и тимол (12.29%), док етарско уље Th. algeriensis карактерише тимол (38.50%) који је главна компонента. Уље Satureja thymbra као водеће компоненте садржи γ- терпинен (39.23%), тимол (25.16%) и p-цимен (7.17%). Висок садржај 1.8- цинеола (49.34%) и камфора (7.53%) је присутан у уљу Salvia fruticosa. Такође је испитивано антиоксидативно дејство уља и главних компонената етарских уља коришћењем DPPH теста. Највиши антиоксидативни потенцијал показало је уље Satureja thymbra (IC50 = 0.0967 mg/ml раствора), затим Thymus capitatus (IC50 = 0.119 mg/ml раствора), Th. algeriensis sa IC50 od 0.299 mg/ml, а најнижа активност добијена је са уљем Salvia fruticosa (IC50 = 15.53 mg/ml раствора). Добијене IC50 вредности за тимол и карвакрол су 0.403 и 0.105 mg/ml, и 0.0717 mg/ml раствора за BHA. Коришћењем методе микродилуције испитивана је антимикробна активност на осам бактерија и осам гљива. Као тест организми коришћено је четири врсте Грам-негативних бактерија - Escherichia coli (ATCC 35210), Pseudomonas aeruginosa (ATCC 27853), Salmonella typhimurium (ATCC 13311), Proteus mirabilis (хумани изолат) и четири Грам- позитивне бактерије – Listeria monocytogenes (NCTC 7973), Bacillus cereus (клинички изолат), Micrococcus flavus (ATCC 10240), и Staphylococcus aureus (ATCC 6538)..." ]
[ "The essentiasl oils composition of some essential o ils from Lamiaceae family wild growing in Libya ( Thymus capitatus, Thymus algeriensis, Satureja thym bra and Salvia furticosa ) have been analyzed using GC and GC-MS. The oils o f Thymus capitatus, Th. algeriensis and Salvia fruticosa were characterised with domination of oxygenated monoterepenes with percentage 87.60%, 54 .67% and 64.89 % respectively. Monoterpene hydrocarbons was the maj or group of the compounds of the oil of S . thymbra represented 58.57% of the total oil. The main compounds of Th. capitatus essential oil were carvacrol (68.19%) followed by thymol (12.29%), while Th. algeriensis essential oil was characterized by thymol (38.50%) as the major component. The oil of S . thymbra was demonstrated by γ - terpinene (39.23%), thymol (25.16%) and p -cymene (7.17%) as the major constituents. S . fruticosa oil was characterized by contained 1,8-cineole (49 .34%) and camphor (7.53%) as the main compounds. The oils wer e also, screened for antioxidant activity by DPPH assay, and compared wi th their main compounds. The highest antioxidant activity showed S. thymbra oil with IC 50 = 0.0967 mg/ml of solution, followed by Th. capitatus (IC 50 = 0.119 mg/ml, Th. algeriensis with the IC 50 of 0.299 mg/ml and the lowest activity was found fo r S. fruticosa (IC 50 = 15.53 mg/ml of solution), comparing with 0.403 and 0.105 mg/ml for thymol and carvacrol, and 0.0717 mg/ml of solution for BHA. Furthermore, anti microbial activity was tested using microdilution method against eight bacteria a nd eight fungal species. Gram- negative from bacteria: Escherichia coli (ATCC 35210), Pseudomonas aeruginosa (ATCC 27853), Salmonella typhimurium (ATCC 13311), Proteus mirabilis (human isolate) and from Gram-positive bacteria: Listeria monocytogenes (NCTC 7973), Bacillus cereus (clinical isolate), Micrococcus flavus (ATCC 10240), and Staphylococcus aureus (ATCC 6538) were used..." ]
application/pdf
en
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc/4.0/" ]
Универзитет у Београду
[ "Thymus cаpitаtus", "Thymus аlgeriensis", "Sаturejа thymbrа", "Sаlviа fruticosа", "етарско уље", "антимикробна активност", "антиоксидативна активност" ]
[ "Thymus capitatus", "Thymus algeriensis", "Satureja thymbra", "Salvia fruticosa", "essential oil", "antimicrobial activity", "antioxidant activity" ]
[ "Састав, анимикробна и антиоксидативна активност етарских уља Thymus algeriensis, Th. capitatus, Salvia fruticosa и Satureja thymbra из Либије", "Composition, antimicrobial and antioxidant activity of the essential oils of Thumus algeriensis, Thymus capitatus, Satureja thymbra and Salvia fruticosa from Libya" ]
[ "" ]
doctoralThesis
[ "BY-NC" ]
Марин, Петар; Јанаћковић, Пеђа; Соковић, Марина; Джамић, Aна; Гиwели, Aбдулхмид Aхмед; Sastav, animikrobna i antioksidativna aktivnost etarskih ulja Thymus algeriensis, Th. capitatus, Salvia fruticosa i Satureja thymbra iz Libije;
[ "http://nardus.mpn.gov.rs/bitstream/id/1589/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1589/Disertacija.pdf", "https://fedorabg.bg.ac.rs/fedora/get/o:7999/bdef:Content/download" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2165" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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[ "" ]
[ "" ]
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[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/11826
123456789-11826.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:20874/bdef:Content/download
no
Bioinformatički modeli za automatsko mapiranje interakcija između proteina kod čoveka
Bioinformatics models for automatic prediction of human protein-protein interaction
[ "Perović, Vladimir" ]
[ "Savić-Pavićević, Dušanka", "Veljković, Nevena", "Perović, Vladimir", "Savić-Pavićević, Dušanka" ]
Šumonja, Neven S.
2020-02-07T10:29:23
[ "2020-02-07T10:29:23", "2020-07-03T08:08:05" ]
2019-11-01
[ "https://nardus.mpn.gov.rs/handle/123456789/11826", "http://eteze.bg.ac.rs/application/showtheses?thesesId=7180", "https://fedorabg.bg.ac.rs/fedora/get/o:20874/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51816975" ]
[ "Interakcije između bioloških makromolekula imaju ključnu ulogu u osnovnim procesima u živim organizmima, posreduju u metaboličkim putevima, putevima prenosa signala, transkripciji, translaciji i drugim ćelijskim i sistemskim procesima. Veliki broj oboljenja uzrokovan je mutacijama proteina u regionima odgovornim za interakciju sa drugim proteinima koje mogu dovesti do ometanja interakcije protein-DNK, promene u obrascima savijanja proteina, novih nepoželjnih interakcija i omogućavanje interakcije protein-patogen. Mapiranje interaktoma, odnosno kompletne mape interakcija protein-protein (IPP) unutar organizma, je od suštinske važnosti za razumevanje kompleksnih molekularnih odnosa unutar živih sistema, kao i za rasvetljavanje raznih patoloških stanja ljudskog organizma. Bioinformatičke metode za automatsko predviđanje IPP, kao suplementi eksperimentalnim metodama za analizu IPP, omogućavaju bolje razumevanje bioloških procesa i funkcija, lakše otkrivanje potencijalnih meta za ciljanu terapiju i smanjenja vremena i troškova razvoja novih terapeutika. U ovoj studiji razvijeni su modeli i metode za automatsko predviđanje IPP bazirane na mašinskom učenju i proteinskoj sekvenci, koja predstavlja univerzalnu, visoko kvalitetnu i eksperimentalno potvrđenu informaciju o proteinu. Generisani su modeli za predviđanje IPP za specijalne slučajeve: (i) između transkripcionih regulatora, odnosno proteina koji učestvuju u kompleksnom procesu transkripcione regulacije koji kontroliše ekspresiju gena i značajan je za normalnu fiziologiju ćelije, i (ii) proteina sa neuređenom tercijarnom strukturom, koji su kao takvi uključeni u ključne biološke procese interakcijom sa višestrukim partnerima, imaju fleksibilnu strukturu, višestruke funkcije, centralnu ulogu u regulaciji signalnih puteva, procesu prepoznavanja i vezivanja za male molekule, i čine većinu proteina povezanih sa neprenosivim bolestima. Pored toga, kreirane su tri nove vrste atributa za predstavljanje proteina: (i) atributi zasnovani na primarnoj strukturi proteina, (ii) evolutivni atributi i (iii) mrežni atributi, kao i metode bazirane na genetskom algoritmu za (i) automatsko generisanje i selekciju atributa i (ii) za automatsko formiranje i optimizaciju ansambla modela zasnovanim na mašinskom učenju, u svrhu proširenja prostora atributa i povećanja efikasnosti predviđanja IPP..." ]
[ "Interactions between biological macromolecules have a critical role in essential processes in living organisms, mediate the metabolic pathways, signaling pathways, transcription, translation and other cellular processes and systems. A large number of diseases caused by mutations in the regions of the protein responsible for the interactions with other proteins which can lead to interference of protein-DNA interaction, changes in the patterns of protein folding, new undesirable interaction and facilitate interaction of the protein-pathogen. Interactome mapping, i.e. mapping of complete network of protein-protein interactions (PPI) within the organism, is essential to an understanding of complex molecular relationships within the living system, as well as to elucidate the various human pathological conditions. Bioinformatics methods for automated PPI prediction, as addition to experimental methods for the analysis of PPI, allow a better understanding of biological processes and functions, easier detection of potential therapeutic targets, and reduce the time and cost of drug development. In this study, models have been developed and methods for the automated prediction of PPI based on machine learning and the protein sequence, which is a universal, high-quality and experimentally confirmed information on the protein. Models for the PPI prediction were generated for special cases: (i) between human transcriptional regulators, i.e. the proteins involved in the complex process of transcriptional regulation that controls the gene expression and they are important for normal cell physiology, and (ii) intrinsically disorder proteins, characterized by the lack of a fixed tertiary structure, which are as such involved in the regulation of key biological processes via binding to multiple protein partners, are malleable adapting to structurally different partners, have multiple functions, play a central roles in the regulation of signaling pathways, the process of molecular recognition and binding of small molecule, and are the prevailing protein class associated with noncommunicable diseases. In addition, three novel types of features for the representation of the proteins were created: (i) the features based on the protein sequence, (ii) the evolutionary features, and (iii) the graph features, as well as methods based on the genetic algorithm for (i) automatic feature-engineering process and (ii) automatic ensembling of different machine learning algorithms, in order to expand the feature space and to improve the PPI prediction performance..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173001/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nd/4.0/" ]
Универзитет у Београду
[ "interakcije protein-protein", "proteom čoveka", "proteinske sekvence", "mašinsko učenje" ]
[ "protein–protein interactions", "human proteome", "protein sequence", "machine learning" ]
[ "Bioinformatički modeli za automatsko mapiranje interakcija između proteina kod čoveka" ]
[ "Bioinformatics models for automatic prediction of human protein-protein interaction" ]
doctoralThesis
[ "BY-ND" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1792/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1793/IzvestajKomisije21836.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1793/IzvestajKomisije21836.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1792/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_11826" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/2090
123456789-2090.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:5439/bdef:Content/download
no
Antitumorski efekat steroidnih tetraoksana na maligno transformisane ćelijske linije čoveka
Antitumor effect of steroidal tetraoxanes on malignantly transformed human cell lines
[ "Radenović, Lidija" ]
[ "Juranić, Zorica", "Šolaja, Bogdan", "Opsenica, Dejan" ]
Žižak, Željko S.
2016-01-05T11:46:45
[ "2016-01-05T11:46:45", "2020-07-03T08:09:28" ]
2012-12-27
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=193", "https://nardus.mpn.gov.rs/handle/123456789/2090", "https://fedorabg.bg.ac.rs/fedora/get/o:5439/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024559794" ]
[ "Uvod: Pronalaženje agenasa sa potencijalnim antitumorskim dejstvom je imperativ u modernoj onkologiji. Pri tome se sve više zapaža da određene hemijske strukture imaju specifičnije toksično dejstvo na maligne ćelije. Otkriće artemizinina je označilo početak istraživanja peroksida kao potencijalne zamene za tradicionalne antimalarijske lekove, a iz ovih istraživanja nastala je i strukturno jednostavna klasa peroksida - 1,2,4,5-tetraoksani, za koje je ubrzo pokazano da pored antimalarijskog pokazuju i snažan antiproliferativni efekat. Maligne ćelije imaju poremećaje u regulatornim mehanizmima koji upravljaju ćelijskom proliferacijom i homeostazom. Sposobnost tumorskih ćelijskih populacija da povećaju broj ćelija je određena ne samo intenzitetom ćelijske proliferacije, već i brzinom uklanjanja ćelija. Programirana ćelijska smrt - apoptoza, predstavlja glavni izvor ovog uklanjanja. Cilj rada je bio da se odredi nivo citotoksičnog dejstva grupe mešovitih tetraoksana prema različitim humanim malignim ćelijama, kao i da se odredi koeficijenat selektivnosti u njihovom dejstvu u odnosu na zdrave imunokompetentne ćelije. U cilju dobijanja uvida u mehanizam dejstva ispitivanih jedinjenja odrediće se tip ćelijske smrti koju indukuju ispitivani tetraoksani, kao i produkcija reaktivnih kiseoničnih vrsta u Hela ćelijama. Cilj 3D QSAR studije o antiproliferativoj aktivnosti trideset tri 1,2,4,5-tetraoksanska derivata prema Hela i Fem-x tumorskim ćelijskim linijama, je bio da se utvrdi koje su najvažnije farmakofore steroidnih tetraoksana koje utiču na potenciju ispitivanih jedinjenja prema HeLa i Fem-x tumorskim ćelijskim linijama. Materijal i metode: Citotoksično dejstvo tetraoksana DO-122 - DO-124 i DO-126 - DO-128, prema pet tumorskih ćelijskih linija je ispitivano standardnim MTT testom. U cilju određivanja tipa ćelijske smrti indukovane tretmanom ispitivanim tetraoksanima načinjena je morfološka analiza HeLa ćelija obojenih smešom akridin oranža i etidijum bromida. Analiza određivanja distribucije faza ćelijskog ciklusa HeLa ćelija obojenih propidijum jodidom, urađena je na protočnom citometru. Produkcija intraćelijskih reaktivnih vrsta kiseonika (ROS) je merena fluorometrijski pomoću fluorescentne boje 2’,7’-dihlorodihidrofluorescein diacetata. Zavisnost strukture i funkcije trideset tri 1,2,4,5-tetraoksanskih derivata prema HeLa i Fem-x ćelijskim linijama pokazana je 3D QSAR studijom..." ]
[ "" ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175011/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Antitumorski lekovi", "apoptoza", "artemizinin", "citotoksičnost", "ćelijski ciklus", "hemioterapija", "peroksidi", "reaktivne vrste kiseonika", "tetraoksani" ]
[ "Anticancer drugs", "apoptosis", "artemisinin", "cytotoxicity", "cell cycle", "chemotherapy", "peroxides", "reactive oxygen species", "tetraoxanes" ]
[ "Antitumorski efekat steroidnih tetraoksana na maligno transformisane ćelijske linije čoveka", "Antitumor effect of steroidal tetraoxanes on malignantly transformed human cell lines" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Раденовић, Лидија; Јуранић, Зорица; Шолаја, Богдан; Опсеница, Дејан; Жижак, Жељко С.; Aнтитуморски ефекат стероидних тетраоксана на малигно трансформисане ћелијске линије човека; Aнтитуморски ефекат стероидних тетраоксана на малигно трансформисане ћелијске линије човека;
[ "http://nardus.mpn.gov.rs/bitstream/id/2101/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/2101/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2090" ]
10.2298/bg20121227zizak
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/2066
123456789-2066.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:3499/bdef:Content/download
no
Ekspresija gena za inhibitore cisteinskih proteinaza (OCI i OCII) u transformisanim biljkama krompira (Solanum tuberosum L.)
Expression of cysteine proteinase inhibitor genes (OCI and OCII) in transformed potato (Solanum tuberosum L.) plants
[ "Ninković, Slavica" ]
[ "Radović, Svetlana", "Lazarević, Jelica", "Miljuš-Đukić, Jovanka" ]
Cingel, Aleksandar
2016-01-05T11:46:20
[ "2016-01-05T11:46:20", "2020-07-03T08:07:17" ]
2012-12-06
[ "https://nardus.mpn.gov.rs/handle/123456789/2066", "http://eteze.bg.ac.rs/application/showtheses?thesesId=91", "https://fedorabg.bg.ac.rs/fedora/get/o:3499/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=42095119" ]
[ "Kombinovanje ili “slaganje” različitih gena u transgenim biljkama radi postizanja uspešnije kontrole patogena i štetočina i/ili većeg prinosa predstavlja jednu od glavnih oblasti istraživanja savremene biotehnologije. Orizacistatini I i II (OCI i OCII), proteinazni inhibitori različitih specifičnosti, pokazali su potencijal u kontroli štetočina koje koriste cisteinske proteinaze za digestiju proteina. Da bi se pojačao njihov inhibitorni potencijal i, eventualno, povećala efikasnost ovih inhibitora u kontroli štetočina, oba cistatina su koeksprimirana u transformisanim biljkama tri sorte krompira. “Slaganje” orizacistatinskih gena kod sorti Dragačevka i Dezire ostvareno je postupkom ko-transformacije i zabeležena je frekvenca kointegracije OCI i OCII gena od 20-22%. Kod sorte Jelica sekvencijalna re-transformacija se pokazala kao efikasniji pristup: frekvenca integracije OCII gena nakon re-transformacije OCI-transformisane linije iznosila je 91%. Istovremeno, “slaganje” dva orizacistatnska gena, bilo postupkom ko- ili re-transformacije, postignuto je upotrebom nptII gena kao jedinog selekcionog markera. Ekspresija OCI i OCII gena indukovana povređivanjem i akumulacija biološki aktivnih rekombinantnih OCI i OCII proteina potvrđena je kod svih analiziranih OCI/OCII transformisanih linija krompira. OCI/OCII linije krompira nisu ispoljavale značajna odstupanja od normalnog fenotipa, što ukazuje na nizak nivo somaklonalnih varijacija i odsustvo uticaja rekombinantnih OCI i OCII na metabolizam biljke domaćina. Iako nije uticala na preživljavanje, ishrana larvi krompirove zlatice (Leptinotarsa decemlineata Say) listovima krompira koji eksprimiraju oba orizacistatina imala je značajan uticaj na različite osobine performanse rasta i razvića larvi. Larve hranjene transformisanim listovima su se presvlačile ranije, i tokom L2 i L3 stupnja uvećavale masu do 29,7% brže i konzumirale listove do 29,1% brže u odnosu na one hranjene netransformisanim listovima. Istovremeno, larve na OCI/OCII listovima su do tri dana ranije dostizale maksimum mase i ranije “usporavale” sa ishranom ulazeći u prepupalnu fazu razvića. Uprkos povećanju performansi rasta i ishrane, pri istoj efikasnosti ishrane, L4 larve na transformisanim listovima nisu u potpunosti uspele da kompenzuju negativne efekte prisustva orizacistatina u hrani. U odnosu na larve hranjene netransformisanim listovima, maksimalna masa na kraju larvenog razvića i ukupan stepen oštećenja listova bili su do 19,4% i do 18,5% manji kod larvi krompirove zlatice hranjenih OCI/OCII transformisanim listovima krompira. Smanjenje mase larvi na OCI/OCII listovima dovelo je i do pojave adulta krompirove zlatice sa do 26,3% redukovanom telesnom masom. Analiza ukupne proteinazne..." ]
[ "The combination or stacking different genes in transgenic plants to achieve disease and pest control and/or higher crop yield is one of a major method of contemporary biotechnology. Oryzacystatins I and II (OCI and OCII), inhibitors with different specificity, show potential in controlling pests that utilize cysteine proteinases for protein digestion. To strengthen this inhibitory range and, possibly, achieve an additive effect in the overall efficiency of these proteins against pests, both cystatins were co-expressed in three potato cultivars. Oryzacystatin genes pyramiding in Dragačevka and Desiree cultivars were achieved by co-transformation with OCI and OCII genes co-integration frequency of 20-22%. For Jelica cultivar sequential re-transformation was more efficient approach: OCII gene integration frequency following re-transformation of an OCI-expressing line was 91%. Additionally, pyramiding of different oryzacystatin genes, by co- or re-transformation approach, were achieved using the nptII gene as the only selection marker. Wounding induction of OCI and OCII gene transcripts and accumulation of biologically active OCI and OCII recombinant proteins was confirmed in all analyzed OCI/OCII transformed lines. OCI/OCII potato lines did not exhibit morphological abnormalities, indicating low level of somaclonal variation or interference of the recombinant OCI or OCII with host plant metabolism. In the absence of significant mortality, feeding Colorado potato beetle larvae (Leptinotarsa decemlineata Say) on OCI/OCII-expressing foliage had an impact on various aspects of the growth and developmental performances of larvae. Larvae feeding on transformed potato leaves tended to molt earlier and, especially during L2-L3 stages, gain weight up to 29.7% faster and consume leaf material up to 29.1% faster, compared to those on untransformed foliage. Larvae on OCI/OCII foliage were also reach maximum weight gained three days earlier and slow down earlier in preparation for pupation. Despite their faster growth and feeding, with similar efficiencies of conversion of ingested food, L4 larvae reared on transformed foliage were not compensating presence of the recombinant oryzacystatins in the diet.Compared to those on untransformed foliage, maximum weight gained and amount of foliage consumed were up to 19.4% and 18.5%, respectively, lower for the larvae fed on OCI/OCII potato foliage. Larval weight reduction on OCI/OCII foliage resulted in adult emergence with up to 26.3% reduced body mass. Analysis of total digestive proteinases activity showed initially, up to 56%, reduction in digestive capacity..." ]
application/pdf
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Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173015/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by/4.0/" ]
Универзитет у Београду
[ "slaganje gena", "ko-transformacija", "re-transformacija", "krompir", "inhibitori cisteinskih proteinaza", "orizacistatin I", "orizacistatin II", "otpornost prema insektima", "krompirova zlatica", "kompenzatorni odgovori insekata" ]
[ "gene stacking", "co-transformation", "re-transformation", "potato", "cysteine proteinase inhibitors", "oryzacystatin I", "oryzacystatin II", "insect resistance", "Colorado potato beetle", "insect compensatory response" ]
[ "Ekspresija gena za inhibitore cisteinskih proteinaza (OCI i OCII) u transformisanim biljkama krompira (Solanum tuberosum L.)", "Expression of cysteine proteinase inhibitor genes (OCI and OCII) in transformed potato (Solanum tuberosum L.) plants" ]
[ "" ]
doctoralThesis
[ "BY" ]
Нинковић, Славица; Радовић, Светлана; Лазаревић, Јелица; Миљуш-Ђукић, Јованка; Цингел, Aлександар; Експресија гена за инхибиторе цистеинских протеиназа (ОЦИ и ОЦИИ) у трансформисаним биљкама кромпира (Соланум туберосум Л.); Експресија гена за инхибиторе цистеинских протеиназа (ОЦИ и ОЦИИ) у трансформисаним биљкама кромпира (Соланум туберосум Л.);
[ "https://nardus.mpn.gov.rs/bitstream/id/1555/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1555/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2066" ]
10.2298/bg20121206cingel
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https://nardus.mpn.gov.rs/handle/123456789/10970
123456789-10970.pdf
yes
yes
https://fedorabg.bg.ac.rs/fedora/get/o:19451/bdef:Content/download
no
Analiza efekta povećane ekspresije SOX2 gena na održavanje pluripotentnosti, proliferaciju i neuralnu diferencijaciju embrionalnih karcinomskih NT2/D1 ćelija
Analysis of effects of SOX2 overexpression on pluripotency, proliferation and neural differentation of embryonal carcinoma NT2/D1 cells
[ "Stevanović, Milena" ]
[ "Brajušković, Goran", "Radović, Svetlana" ]
Drakulić, Danijela D.
2019-04-22T11:59:01
[ "2019-04-22T11:59:01", "2020-07-03T08:07:42" ]
2011
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6638", "https://nardus.mpn.gov.rs/handle/123456789/10970", "https://fedorabg.bg.ac.rs/fedora/get/o:19451/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=40172047" ]
[ "SOX2 transkripcioni faktor predstavlja univerzalni marker pluripotentnih matičnih ćelija. Ovaj faktor poseduje značajne uloge tokom embrionalnog razvića kičmenjaka i jedan je od ključnih regulatora neuralne diferencijacije. Takođe, SOX2 transkripcioni faktor u različitim ćelijama promoviše ili reprimira ćelijsku proliferaciju. NT2/D1, pluripotentna embrionalna karcinomska ćelijska linija koja vodi poreklo od malignog testikularnog teratokarcinoma, je slična ranim embrionalnim matičnim ćelija. U prisustvu retinoične kiseline ove ćelije se diferenciraju u različite tipove ćelija, uključujući i zrele neurone. Imajući u vidu značajnu ulogu SOX2 gena u održavanju pluripotentnosti, proliferaciji i neuralnoj diferencijaciji, u ovoj disertaciji analiziran je uticaj povećane ekspresije ovog gena na ekspresiju markera pluripotentnosti, proliferaciju i neuralnu diferencijaciju NT2/D1 ćelija. Stabilnom transfekcijom NT2/D1 ćelija SOX2/pcDNA3.1 konstruktom generisana su dva ćelijska klona sa povećanom ekspresijom SOX2 gena (klonovi su označeni kao F5 i G3). Ovom metodom generisana su i tri NT2/D1 ćelijska klona sa inducibilnom ekspresijom SOX2 gena kod kojih je ekspresija ovog gena regulisana različitih koncentracija doksiciklina (klonovi su označeni kao E2, H9 i I7). Rezultati semi- kvantitativnog RT-PCR- a ukazuju da povećana ekspresija SOX2 gena dovodi do promene ekspresije ključnih markera pluripotentnosti (OCT4 i NANOG). Takođe, NT2/D1 ćelijski klonovi sa povećanom ekspresijom SOX2 gena (F5 i G3) ulaze u neuralnu diferencijaciju indukovanu retinoičnom kiselinom čak i u uslovima povećane ekspresije SOX2 gena. Tretman retinoičnom kiselinom u trajanju od tri nedelje doveo je do povećanja ekspresije markera neuralne diferencijacije (neuroD1 i sinaptofizin) kod NT2/D1 ćelijskih klonova sa povećanom ekspresijom SOX2 gena u poređenju sa ekspresijom ovih markera detektovanom kod tretiranih NT2/D1 ćelija. Testom za određivanje proliferativnog kapaciteta ćelija (MTT testom) je pokazano da je stopa rasta G3 ćelijskog klona povećana približno 1,3 puta u poređenju sa stopom rasta NT2/D1 ćelija. U ovoj disertaciji su po prvi put prikazani rezultati koji pokazuju da povećana ekspresija SOX2 gena kod NT2/D1 ćelija dovodi do promene ekspresije ključnih markera pluripotentnosti, do promene proliferacije ovih ćelija, kao i do promene ekspresije markera neuralne diferencijacije nakon tri nedelje tretmana retinoičnom kiselinom." ]
[ "SOX2, an universal marker of pluripotent stem cells, is a transcription factor that plays significant roles during embryonic development in vertebrates and it is one of the key regulators of neural differentiation. Furthermore, in different cellular contexts this transcription factor represses or promotes proliferation. NT2/D1, pluripotent embryonal carcinoma cell line originating from malignant testicular teratocarcinomas, resembles early embryonic stem cells. In the presence of retinoic acid, these cells differentiate into various cell types, including mature neurons. Given the critical role of SOX2 transcription factor in regulation of pluripotency, cell proliferation and neural differentiation, our aim has been to analyze effects of SOX2 overexpression on expression of key pluripotency genes, cell proliferation and expression of neural markers during retinoic acid treatment of NT2/D1 cell line. Two SOX2-overexpressing NT2/D1 cell clones (designated as F5 and G3) have been generate by stable transfection using SOX2/pcDNA3.1 expression construct. Furthermore, we have generated three inducible SOX2-overexpressing NT2/D1 cell clones (designated as E2, H9 and I7) in which SOX2 expression is regulated by various concentrations of doxycycline. Results of semi-quantitative RT-PCR analysis demonstrated that SOX2 overexpression in NT2/D1 cell clones resulted in altered expression of key pluripotency genes OCT4 and NANOG. Furthermore, SOX2-overexpressing NT2/D1 cell clones (F5 and G3 cell clones) are able to enter into RA-dependent neural differentiation even in the presence of elevated SOX2 expression. It is shown that upon three weeks of treatment by retinoic acid, expression of neural markers (neuroD1 and synaptophysin) is higher in treated cell clones compared to treated parental NT2/D1 cells. In addition, results of cell proliferation assay (MTT test) demonstrated higher growth rate of G3 cell clone compared to the growth rate of parental cells. By data presented here, for the first time, we demonstrated that SOX2 overexpression in NT2/D1 cell line resulted in altered expression of pluripotency-related genes and proliferation rate and in altered expression of neural markers after three weeks of treatment by retinoic acid." ]
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Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "" ]
Универзитет у Београду
[ "SOX2", "NT2/D1", "pluripotentnost", "proliferacija ćelija", "neuralna diferencijacija" ]
[ "SOX2", "NT2/D1", "pluripotency", "cell proliferation", "neural differentiation" ]
[ "Analiza efekta povećane ekspresije SOX2 gena na održavanje pluripotentnosti, proliferaciju i neuralnu diferencijaciju embrionalnih karcinomskih NT2/D1 ćelija" ]
[ "Analysis of effects of SOX2 overexpression on pluripotency, proliferation and neural differentation of embryonal carcinoma NT2/D1 cells" ]
doctoralThesis
[ "ARR" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1672/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1672/Disertacija.pdf", "https://fedorabg.bg.ac.rs/fedora/get/o:19451/bdef:Content/download" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10970" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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[ "" ]
[ "" ]
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[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/5666
123456789-5666.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:11286/bdef:Content/download
no
Metaboličke karakteristike sindroma policističnih jajanika u visceralnom masnom tkivu i leptinska rezistencija u hipotalamusu pacova tretiranog 5alfa-dihidrotestosteronom: uloga glukokortikosteroida
Metabolic characteristics of polycistic ovary syndrom in the visceral adipose tissue and leptin resistance in the hypothalamus of the rat treated with 5alfa-dihydrotestosterone: role of glycocorticoids
[ "Vojnović-Milutinović, Danijela" ]
[ "Matić, Gordana", "Macut, Đuro", "Vojnović-Milutinović, Danijela", "Matić, Gordana" ]
Nikolić, Marina M.
2016-07-10T17:07:23
[ "2016-07-10T17:07:23", "2020-07-03T08:07:35" ]
2016-02-26
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=3045", "https://nardus.mpn.gov.rs/handle/123456789/5666", "https://fedorabg.bg.ac.rs/fedora/get/o:11286/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025053106" ]
[ "Sindrom policističnih jajnika (PCOS) je najčešća endokrinopatija žena u reproduktivnom životnom dobu i predstavlja heterogeno oboljenje, koje u osnovi karakterišu hiperandrogenizam, hronična anovulacija i policistični jajnici, a sa njim su povezane i karakteristike metaboličkog sindroma, kao što su insulinska rezistencija, gojaznost, dislipidemija i hipertenzija. Patofiziologija i etiologija PCOS-a, odnosno uzročno-posledična povezanost njegovih pojedinačnih simptoma, nisu do kraja razjašnjene. Generalno se smatra da reproduktivni, endokrini i metabolički poremećaji u PCOS-u formiraju začarani krug u čijem centru je hiperandrogenemija, koju stimulišu visceralna gojaznost, narušen lipidni metabolizam, inflamacija i insulinska rezistencija. Glukokortikoidni hormoni utiču na pojavu visceralne gojaznosti i, delujući antagonistički sa insulinom, mogu da doprinesu patofiziologiji metaboličkog sindroma. Glukokortikoidni hormoni tkivno-specifičnim efektima podstiču nastanak insulinske rezistencije na nivou organizma. Imaju i generalno antiinflamatorna dejstva, ali u gojaznosti podstiču ekspresiju nekih proinflamatornih činilaca. Leptin je hormon masnog tkiva, čija je najvažnija uloga centralno anoreksigeno dejstvo u hipotalamusu tokom kontrole unosa energije. Pri tome, leptin deluje sinergistički sa insulinom i antagonistički sa glukokortikoidnim hormonima. U gojaznosti dolazi do hiperleptinemije i nemogućnosti leptina da ostvari svoje efekte, to jest razvoja leptinske rezistencije. Glukokortikoidi kroz interakcije sa leptinskim signalnim putem mogu da podstaknu njen nastanak..." ]
[ "Polycystic ovary syndrome (PCOS) is the most common endocrinopathy of women of reproductive age. It is a heterogenous disorder, with hyperandrogenism, chronic anovulation and polycystic ovaries as basic characterisctics, and features of the metabolic syndrome, such as insulin resistance, obesity, dislipidemia and hypertension as associated metabolic characteristics. Pathophysiology and aetiology of PCOS and interlinks between its symptoms are yet to be clarified. It is generally considered that the reproductive, endocrine and metabolic features of PCOS create a vicious circle in the centre of which lies hyperandrogenemia, stimulated by visceral obesity, disturbed lipid metabolism, inflammation and insulin resistance. By functioning antagonistically with insulin, glucocorticoids influence the genesis of visceral obesity and contribute to the pathophysiology of the metabolic syndrome. Their tissue-specific effects stimulate systemic insulin resistance. In addition, glucocorticoids generally exhibit antiinflamatory actions. In obesity, however, they show an ability to stimulate proinflammatory factors. Leptin is a hormone with anorexigenic effects in the hypothalamus. In the course of energy intake and consumption control it works synergistically with insulin, but antagonizes the actions of glucocorticoids. Increased secretion of leptin - hyperleptinemia and leptin resistance are common consequences of obesity..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/178021/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]
Универзитет у Београду
[ "PCOS", "DHT", "visceralno masno tkivo", "hipotalamus", "glukokortikoidni hormoni", "leptin", "insulin", "inflamacija" ]
[ "PCOS", "DHT", "visceral adipose tissue", "hypothalamus", "glucocorticoids", "leptin", "insulin", "inflammation" ]
[ "Metaboličke karakteristike sindroma policističnih jajanika u visceralnom masnom tkivu i leptinska rezistencija u hipotalamusu pacova tretiranog 5alfa-dihidrotestosteronom: uloga glukokortikosteroida", "Metabolic characteristics of polycistic ovary syndrom in the visceral adipose tissue and leptin resistance in the hypothalamus of the rat treated with 5alfa-dihydrotestosterone: role of glycocorticoids" ]
[ "" ]
doctoralThesis
[ "BY-NC-SA" ]
Војновић-Милутиновић, Данијела; Матић, Гордана; Матић, Гордана; Војновић-Милутиновић, Данијела; Мацут, Ђуро; Николић, Марина М.; Метаболичке карактеристике синдрома полицистичних јајаника у висцералном масном ткиву и лептинска резистенција у хипоталамусу пацова третираног 5алфа-дихидротестостероном: улога глукокортикостероида; Метаболичке карактеристике синдрома полицистичних јајаника у висцералном масном ткиву и лептинска резистенција у хипоталамусу пацова третираног 5алфа-дихидротестостероном: улога глукокортикостероида;
[ "https://nardus.mpn.gov.rs/bitstream/id/1645/Disertacija3586.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1646/Marina_Nikolic_Referat_BF.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1645/Disertacija3586.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1646/Marina_Nikolic_Referat_BF.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_5666" ]
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https://nardus.mpn.gov.rs/handle/123456789/10048
123456789-10048.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:18378/bdef:Content/download
no
Potencijalna uloga gubara (Lymantria dispar, L.) kao bioindikatora zagađenosti životne sredine: uticaj hroničnog izlaganja kadmijumu na razviće, aktivnosti digestivnih enzima srednjeg creva, mehanizme detoksifikacije i stepen oštećenja DNK
Potential role of gypsy moth (Lymantria dispar, L.) as a bioindicator of environmental pollution: the impact of cadmium chronic exposure on the development, midgut digestive enzyme activities, detoxification mechanisms and DNA damage level
[ "Vlahović, Milena" ]
[ "Đurašević, Siniša", "Perić Mataruga, Vesna", "Ilijin, Larisa", "Kolarević, Stoimir" ]
Matić, Dragana R.
2018-11-07T15:53:10
[ "2018-11-07T15:53:10", "2020-07-03T08:06:57" ]
2018-06-07
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6044", "https://nardus.mpn.gov.rs/handle/123456789/10048", "https://fedorabg.bg.ac.rs/fedora/get/o:18378/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025186482" ]
[ "Razvoj industrije i poljoprivrede, kao i globalna urbanizacija, doveli su do pojave velikog broja polutanata u životnoj sredini, među kojima je i izuzetno toksični teški metal kadmijum. Cilj ove disertacije bila je procena larvi gubara (Lymantria dispar, L.) kao potencijalnog bioindikatora zagađenosti kadmijumom. Ispitani su odgovori izabranih fizioloških parametara na različitim nivoima biološke organizacije u prisustvu dve koncentracije metala (50 i 100 μg Cd/g suve hrane). Radi ispitivanja efekta porekla populacije, legla gubara sakupljena su na dve nezagađene lokacije (Kosmaj i Homoljske planine) i dve zagađene (pored Ibarske magistrale i borskog rudnika). Larve sa Kosmaja odlikuje veća osetljivost digestivnih enzima srednjeg creva (proteaza, alkalnih i kiselih fosfataza i nespecifičnih esteraza) na tretmane, naročito na nižu koncentraciju kadmijuma, u odnosu na one uz Ibarsku magistralu. Od ispitanih detoksifikacionih mehanizama u crevu, indukcija Hsp70 značajnija je kod kosmajskih larvi, a sinteza metalotioneina kod ibarskih. Trend sniženja aktivnosti antioksidativnih enzima, superoksid dismutaze i katalaze, uočen je kod obe populacije. U svim grupama tretiranim kadmijumom najveća količina ovog metala eliminiše se putem fecesa, ali intenzivnije u ibarskoj populaciji u odnosu na kosmajsku nakon tretmana sa 50 μg Cd/g suve hrane. Kod homoljskih gusenica tretmani kadmijumom doveli su do povećanja nivoa oštećenja DNK u hemocitama i dozno-zavisnog sniženja njihove vijabilnosti, dok je u populaciji iz Bora zabeleženo samo smanjenje vijabilnosti nakon primene više koncentracije metala. Štetni efekti kadmijuma i visoka cena detoksifikacionih mehanizama odrazili su se i na osobine fitnesa u vidu promena mase larvi, relativne brzine rasta i trajanja razvića kod svih populacija u različitom stepenu. Primena većine ispitanih parametara (specifične aktivnosti tripsina, nelizozomskih kiselih fosfataza i nespecifičnih esteraza, nivoa ekspresije Hsp70, vijabilnosti hemocita i nivoa oštećenja DNK) kao biomarkera zagađenosti životne sredine kadmijumom ograničena je na osetljive populacije gubara koje prethodno nisu bile izložene polutantima, dok bi koncentracija metalotioneina mogla biti podobna kao biomarker kod populacija sa lokacija kontaminiranih tokom dužeg perioda." ]
[ "considerable increase in the environmental pollution including extremely toxic heavy metal cadmium. The aim of this dissertation was to evaluate gypsy moth (Lymantria dispar, L.) larvae as bioindicators of cadmium contamination. Parameters at different levels of biological organization were assessed after chronic exposure of larvae to the metal (50 and 100 μg Cd/g dry food). To investigate the effects of population origin, gypsy moth egg-masses were collected from two unpolluted localities on Kosmaj and Homolje mountains and two polluted sites near the busy Ibar highway and Bor copper mine. Midgut digestive enzymes (proteases, alkaline and acid phosphatases and nonspecific esterases) of Kosmaj larvae were more sensitive to cadmium, especially to lower metal concentration, compared to those of Ibar larvae. Hsp70 induction was the most prominent midgut detoxification mechanism against cadmium in the population from the unpolluted forest, whereas metallothionein synthesis was the most significant in larvae from the polluted site. Activities of antioxidative enzymes, superoxide dismutase and catalase, exhibited a decreasing trend in both populations. The primary route of cadmium elimination was via feces in all metal-fed groups, but after an exposure to 50 μg Cd/g dry food metal content was significantly higher in feces of Kosmaj larvae compared to those from the highway locality. Exposure of caterpillars from the Homolje mountains to the metal led to the higher level of DNA damage in the hemocytes and a dose-dependent decrease in cell viability, whereas in Bor population only hemocyte viability was reduced after the treatment with 100 μg Cd/g dry food. Devastating cadmium effects and energetically expensive detoxification mechanisms affected the fitness traits (larval mass, relative growth rate or development parameters) in all populations. The application of selected parameters (specific activities of trypsin, nonlisosomal acid phosphatases and nonspecific esterases, Hsp70 expression, hemocyte viability and DNA damage level) as biomarkers of environmental cadmium pollution is limited mostly to sensitive populations that have not been previously exposed to pollutants. Metallothionein concentration might be suitable as a biomarker for the populations at locations that have been contaminated for a long period." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173027/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]
Универзитет у Београду
[ "Lymantria dispar", "L.", "kadmijum", "digestivni enzimi", "antioksidativni enzimi", "Hsp70", "metalotionein", "akumulacija kadmijuma u tkivima", "genotoksičnost", "osobine fitnesa", "biomarkeri" ]
[ "Lymantria dispar", "L.", "cadmijum", "digestive enzymes", "antioxidative enzymes", "Hsp70", "metallothionein", "cadmium accumulation in tissues", "genotoxicity", "fitness traits", "biomarkers" ]
[ "Potencijalna uloga gubara (Lymantria dispar, L.) kao bioindikatora zagađenosti životne sredine: uticaj hroničnog izlaganja kadmijumu na razviće, aktivnosti digestivnih enzima srednjeg creva, mehanizme detoksifikacije i stepen oštećenja DNK" ]
[ "Potential role of gypsy moth (Lymantria dispar, L.) as a bioindicator of environmental pollution: the impact of cadmium chronic exposure on the development, midgut digestive enzyme activities, detoxification mechanisms and DNA damage level" ]
doctoralThesis
[ "BY-NC-SA" ]
Влаховић, Милена; Ђурашевић, Синиша; Коларевић, Стоимир; Илијин, Лариса; Перић Матаруга, Весна; Матић, Драгана Р.;
[ "http://nardus.mpn.gov.rs/bitstream/id/1482/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1483/IzvestajKomisije17835.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1483/IzvestajKomisije17835.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1482/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10048" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/10053
123456789-10053.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:18540/bdef:Content/download
no
Uticaj biljke domaćina na kriptičnu diferencijaciju populacija vektora Hyalesthes obsoletus Signoret, 1865 (Hemiptera: Cixiidae) i epidemiološke puteve prenošenja 'Candidatus Phytoplasma solani'
Cixiidae) and on epidemiological transmission routes of 'Candidatus Phytoplasma solani'
[ "Tomanović, Željko" ]
[ "Jović, Jelena", "Toševski, Ivo" ]
Kosovac, Andrea M.
2018-11-07T15:53:14
[ "2018-11-07T15:53:14", "2020-07-03T08:07:04" ]
2018-09-18
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6105", "https://nardus.mpn.gov.rs/handle/123456789/10053", "https://fedorabg.bg.ac.rs/fedora/get/o:18540/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025193650" ]
[ "Hyalesthes obsoletus, vektor biljnog patogena 'Candidatus (Ca.) Phytoplasma solani', smatra se polifagnom štetočinom od ekonomskog značaja u centralnoj Evropi i Mediteranu. Istraživanjima sprovedenim u jugoistočnoj Evropi, koja gravitira centru arela vrste i gde je zabeležen značajan broj 'Ca. P. solani'- uslovljenih biljnih bolesti, utvrđeno je postojanje četiri asocijacije vektora sa biljkama dualnim domaćinima: Convolvulus arvensis (Ca), Urtica dioica (Ud), Vitex agnus-castus (Vac) i Crepis foetida (Cf). Populaciono-genetičkim analizama mitohondrijskih i nuklearnih markera (mikrosatelita) utvrđena je kriptična diferencijacija simpatričkih (uključujući sintopske) i geografski udaljenih H. obsoletus populacija na tri filogenetičke linije uslovljene biljkom domaćinom: (1) Ca-Ud, (2) Vac i (3) Cf. Pored jasne diferencijacije na mitohondrijskim genima (1.1% - 1.5%) i mikrosatelita koji potvrđuju izdvajanje populacija u jedan od tri klastera prema biljci domaćinu (pripadnost >90%), podršku za izdizanje genetičke divergencije H. obsoletus sensu lato na nivo kriptične specijacije daje registrovana distanca u odnosu na morfološki najsrodniju vrstu - Hyalesthes thracicus (2.1% - 3.3%). Posledice specijalizacije i diferencijacije H. obsoletus sensu lato prema biljkama domaćinima na epidemiologiju 'Ca. P. solani' procenjene su multigenskom karakterizacijom izolata fitoplazme detektovanih (1) in situ u prirodnim epidemiološkim sistemima mediteranskih vinograda i (2) eksperimentima unakrsne inokulacije biljaka putem prirodno inficiranih populacija vektora. Potvrđena je osnovna epidemiološka divergencija 'Ca. P. solani' na tuf-a/-ab i tuf-b cikluse vezane za Ud i Ca uz ukrštanje sa Vac-uzrokovanom tuf-b epidemiologijom i utvrđena kompleksnost tuf-b epidemiologije u kojoj učestvuje i treća genetička linija vektora, H. obsoletus ex Cf, kao i sama biljka dualni domaćin - C. foetida." ]
[ "The cixiid planthopper Hyalesthes obsoletus is generally considered as a polyphagous vector of 'Candidatus (Ca.) P. solani' associated with numerous wild and cultivated plants in central Europe and Mediterranean. Research conducted in southeastern Europe, the distribution centre of H. obsoletus and the area of most 'Ca. P. solani'-inflicted crop diseases, points toward specific dual host-plant associations with: Convolvulus arvensis (Ca), Urtica dioica (Ud), Vitex agnus-castus (Vac) and Crepis foetida (Cf). Population-genetic analysis of mitochondrial and nuclear genetic markers (microsatellites) have revealed cryptic differentiation between sympatric (including syntopic) and geographically distant H. obsoletus populations into three phylogenetic lineages delineated by host-plant preference: 1) Ca-Ud, 2) Vac i 3) Cf. Cryptic species diversification within H. obsoletus sensu lato is supported by the genetic divergence of mitochondrial genes (1.1% - 1.5%), microsatellites that confirm population clustering into three genetic groups associated with specific host-plant (average assignment >90%) and genetic distances among host-associated H. obsoletus metapopulations comparable to the most closely related, morphologically distinguishable species - Hyalesthes thracicus (2.1% - 3.3%). Consequences of the H. obsoletus sensu lato differentiation on the epidemiology of 'Ca. P. solani' are assessed by multilocus genotyping of the phytoplasma isolates detected (1) in situ in natural epidemiological systems of the Mediterranean vineyards and (2) plant inoculation experiments using naturally infected vector populations. Basic 'Ca. P. solani' epidemiological divergence into tuf-a/-ab and tuf-b cycles, each associated with Ud and Ca, respectively, is confirmed with evidences of intermixing Vac-induced tuf-b epidemiology, while complexity of the tuf-b epidemiology is further evidenced through influence of the third genetic lineage of the vector H. obsoletus ex C. foetida and its dual host-plant." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43001/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Hyalesthes obsoletus", "biljka domaćin", "populaciona genetika", "kriptična evolucija", "stolbur fitoplazma", "epidemiologija", "multigenska karakterizacija" ]
[ "Hyalesthes obsoletus", "host-plant", "population genetics", "cryptic evolution", "stolbur phytoplasma", "epidemiology", "multilocus genotypisation" ]
[ "Uticaj biljke domaćina na kriptičnu diferencijaciju populacija vektora Hyalesthes obsoletus Signoret, 1865 (Hemiptera: Cixiidae) i epidemiološke puteve prenošenja 'Candidatus Phytoplasma solani'" ]
[ "Cixiidae) and on epidemiological transmission routes of 'Candidatus Phytoplasma solani'" ]
doctoralThesis
[ "BY-NC-ND" ]
Томановић, Жељко; Тошевски, Иво; Јовић, Јелена; Косовац, Aндреа М.;
[ "https://nardus.mpn.gov.rs/bitstream/id/1521/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1522/IzvestajKomisije17877.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1521/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1522/IzvestajKomisije17877.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10053" ]
[ "" ]
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[ "" ]
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[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/3299
123456789-3299.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:8484/bdef:Content/download
no
Српска меморијална архитектура 1918-1955
Serbian memorial architecture 1918-1955
[ "Kadijević, Aleksandar" ]
[ "Makuljević, Nenad", "Ignjatović, Aleksandar", "Manojlović-Pintar, Olga" ]
Obrenović, Violeta N.
2016-01-05T12:54:10
[ "2016-01-05T12:54:10", "2020-07-03T10:03:57" ]
2013-11-08
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=1363", "https://nardus.mpn.gov.rs/handle/123456789/3299", "https://fedorabg.bg.ac.rs/fedora/get/o:8484/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=45401871" ]
[ "Радом Српска меморијална архитектура 1918-1955 обухваћен је релативно дуг временски период у оквиру кога су представљени разноврсни видови меморијалног градитељства, под којим се у ширем смислу подразумевају обележја, објекти, гробља, надгробници, просторне површине, историјски објекти и целине, који чувају успомену на историјске догађаје, личности и пале ратнике. Развој српског меморијалног градитељства испраћен је кроз два различита друштвено-политичка система, две државе- Краљевину Југославију и социјалистичку Југославију, у чијем саставу се налазила Србија у периоду 1918-1955. Период окупације 1941-1944 обухваћен је кроз једино репрезентативно дело меморијалног градитељства подигнуто у овом периоду, Спомен-костурницу у Смедереву. Краљевина СХС - Југославија у чији састав су ушле словенске земље некадашњи делови аустроугарске своју политику заснивала је на националној и верској равноправности свих народа. Уређење српских војничких гробаља, подизање меморијала посвећених српској војсци, и поред великих заслуга које је српска војска стекла у Првом светском рату, држава је спроводећи политику равноправности свих југословенских народа препустила српским националним удружењима, организацијама и посебно формираним oдборима. Држава је показала иницијативу за уређење појединих војничких гробаља у иностранству и подигла неколико меморијала. У међуратном периоду српскo меморијално градитељство, и поред недовољног ангажовања Државе, доживљава експанзију. У овом периоду долази до интезивног уређивања војничких гробаља и подизања костурница са црквама, капелама, маузолејима или архитектонским споменицима над њима. Уређење војничких гробаља је по први пут код нас законски регулисано што је дало посебан значај овом виду меморисања. У овом периоду кроз подизање споменика Незнаном јунаку и намеру да се подигне спомен-храм типа Пантеона, прихваћени су нови видови меморисања из европске меморијалне архитектуре. Поред репрезентативних архитектонских меморијала подигнутих на Зебрњаку, Церу, Гучеву, Споменика браниоцима Београда на Новом гробљу и других, посебан национални значај по бројности имају и други видови меморисања; обелисци, чесме, спомен-обележја, које је обично подизала локалана заједница. У међуратном периоду по први пут меморијална архитектура је прихваћена од стране архитеката и друштва као значајан вид ставралаштва, што су потврдиле изложбе студената архитектуре и Салони архитектуре. У меморијалном градитељству посебно су се истакле архитекте које су радиле при Министарству грађевина, Момир Коруновић, Николај Краснов, Василиј Андросов, као и самосталне архитекте Роман Верховској и Александар Дероко, док је значајан опус који се састоји од идејних пројеката остварио архитекта Милутин Борисављевић. Меморијали су извођени претежно у српско-византијском стилу или под утицајима академизма, мада су приметни и утицаји романтичарског експресионизма..." ]
[ "The work Serbian memorial architecture 1918-1955 covers a relatively long period of time, during which various forms of memorial construction were undertaken. In the broadest sense of the word, this covers emblems, buildings, cemeteries, graves, areas of land, and historical buildings and entities, all preserving the memory of historical events, historical figures and fallen soldiers. The development of Serbian memorial construction is presented through two differing socio-political systems, in two states – the Kingdom of Yugoslavia and socialist Yugoslavia, which Serbia was a part of in the period 1918-1955. The period of occupation (1941-44) is represented by the only grandiose work of this time, the Memorial Vault in Smederevo. The Kingdom of Slovenes, Croats and Serbs – Yugoslavia, comprising Slavic countries that were once part of Austria-Hungary, based its politics on the national and religious equality of all peoples. Despite the enormous praise the Serbian army earned during the First World War, the state, applying its policy of equality of all Yugoslav peoples, left the restoration of Serbian military cemeteries and the erecting of memorials dedicated to the Serbian army to Serbian national associations, organisations and specially formed committees. The state however did show willing to restore certain military cemeteries abroad and erected several memorials. During the inter-war period, Serbian memorial construction increased, despite the lack of state involvement. This period was marked by the intensive restoration of military cemeteries and the building of vaults with churches, chapels, mausoleums or architectural memorials above them. The restoration of military cemeteries was regulated by law for the first time in the country’s history, which gave special significance to this form of memorial. During this period, with the erection of memorials to the Unknown Soldier and the intention to build a memorial temple like the Pantheon, new forms of memorialising from European memorial architecture were accepted. Besides the grandiose architectural memorials erected at Zebrnjak, Cer, and Gučevo, the Monument to the Defenders of Belgrade at New Cemetery and others, other forms of memorial are of special national significance due to their number: obelisks, drinking fountains, and memorial emblems, which were often erected by local organisations. It was in the inter-war period that memorial architecture was accepted for the first time by architects and society as a significant form of creation, confirmed by the exhibitions of architecture students and the Salons of Architecture. Architects who worked for the Ministry of Civil Engineering - Momir Korunović, Nikolaj Krasnov, and Vasilij Androsov - excelled in particular in memorial architecture, as well as the freelance architects, Roman Verhovskoy and Aleksandar Deroko, while the architect Milutin Borisavljevic compiled a significant oeuvre comprising concept designs. Memorials were created mainly in the Serbian-Byzantine style or influenced by academism, although influences of Romantic Expressionism are also evident..." ]
application/pdf
sr
Универзитет у Београду, Филозофски факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "меморијална архитектура", "костурница", "споменик", "војничко гробље", "спомен-црква", "спомен-капела", "рат", "пали ратници" ]
[ "memorial architecture", "vault", "monument", "military cemetery", "memorial church", "memorial chapel", "war", "fallen soldiers" ]
[ "Српска меморијална архитектура 1918-1955", "Serbian memorial architecture 1918-1955" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Кадијевић, Aлександар; Игњатовић, Aлександар; Манојловић-Пинтар, Олга; Макуљевић, Ненад; Обреновић, Виолета Н.; Srpska memorijalna arhitektura 1918-1955;
[ "https://nardus.mpn.gov.rs/bitstream/id/27570/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/27570/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_3299" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/2054
123456789-2054.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:2127/bdef:Content/download
no
Učestalost i tipovi mutacija u genima BRCA u porodicama sa pozitivnom istorijom za karcinom dojke i/ili jajnika u Srbiji
Frequency and types of mutations in BRCA genes in families with positive history of breast/ovarian cancer in Serbia
[ "Stamenković-Radak, Marina" ]
[ "Branković-Magić, Mirjana", "Radulović, Siniša" ]
Dobričić, Jelena D.
2016-01-05T11:46:12
[ "2016-01-05T11:46:12", "2020-07-03T08:08:54" ]
2012-05-23
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6", "https://nardus.mpn.gov.rs/handle/123456789/2054", "https://fedorabg.bg.ac.rs/fedora/get/o:2127/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=" ]
[ "Uvod: Proteinski produkti gena BRCA1 i BRCA2 uključeni su u važne ćelijske procese, kao što su kontrola ćelijskog ciklusa i popravka oštećenja DNK. Mutacije u jednom od ovih gena predstavljaju korak bliže gubitku kontrole nad genomskom stabilnošću i ćelijskom deobom, pa osobe koje naslede mutacije u ovim genima češće oboljevaju od karcinoma dojke i/ili jajnika. Geni BRCA su veliki, mutacije su raspoređene duž celih gena bez grupisanja, što dodatno otežava njihovu detekciju. Spektar mutacija u genima BRCA različit je u različitim etničkim grupama. U nekim populacijama sa visokom učestalošću zastupljene su mutacije koje su u drugim populacijama retke. U isto vreme, veliki deo mutacija u genima BRCA detektovane su samo jednom, pa se može reći da većina porodica pod rizikom ima svoju sopstvenu porodičnu mutaciju. Mutacija 5382insC u 20. egzonu gena BRCA1 karakteristična je za slovenske populacije i njena učestalost opada od istoka ka zapadu Evrope. Oko 6-7% svih karcinoma dojke i oko 10% svih karcinoma jajnika je nasledno, povezano sa mutacijama germinativnih ćelija u genima BRCA1 i BRCA2. Nosioci mutacija u genima BRCA nose 5 do 8 puta veći životni rizik za oboljevanje od karcinoma dojke i 10 do 20 puta veći životni rizik za karcinom jajnika. Cilj istraživanja je bio da se otkriju najučestalije mutacije u genima BRCA1/2 u našoj populaciji i utvrdi koje se od njih mogu smatrati osnivačkim mutacijama, kako bi se ubrzalo, pojednostavilo i pojeftinilo BRCA testiranje. Da bi se ovaj cilj ostvario, bilo je neophodno utvrditi tipove i učestalosti mutacija u genima BRCA1 i BRCA2 kod osoba sa pozitivnom porodičnom istorijom za karcinom dojke i/ili jajnika u Srbiji. Osim toga, u sistematskom uzorku karcinoma dojke bilo je potrebno utvrditi učestalost mutacije 5382insC u genu BRCA1 kao potencijalne osnivačke mutacije u našoj populaciji. U ispitivanoj grupi osoba koje su testirane na prisustvo mutacija u genima BRCA1/2 procenjivana je i efikasnost predviđanja BRCAPRO programa. Materijal i metode: Analizirano je 85 uzoraka periferne krvi osoba sa povećanim rizikom za karcinom dojke i/ili jajnika koje potiču iz 69 porodica. Takođe, analiziran je i sistematski uzorak karcinoma dojke koji se sastoji od 257 uzoraka krvi obolelih. Prisustvo mutacija u genima BRCA analizirano je automatskim sekvenciranjem, dok je prisustvo mutacije 5382insC u genu BRCA1 u sistematskom uzorku određivano PCR-om specifičnim za alel. Za statističku obradu podataka korišćeni su testovi neparametrijske statistike: χ2 test i Fišerov test. Rezultati: Učestalost oštećujućih mutacija u uzorku porodica sa naslednim karcinomom dojke i/ili jajnika iznosi 10,59% (9/85). Sve detektovane oštećujuće mutacije su po tipu frameshift mutacije. Detektovane su i 3 nove porodično-specifične mutacije (1 u genu BRCA1 i 2 u genu BRCA2). Učestalost mutacije 5382insC u genu BRCA1 u sistematskom uzorku karcinoma dojke je 0,39% (1/257). Podizanjem granice BRCAPRO verovatnoće na 40% moguće je uočiti razliku između benignih polimorfizama i oštećujućih mutacija. Nije bilo moguće subgrupisanje neklasifikovanih varijanti na osnovu vrednosti BRCAPRO verovatnoće. BRCAPRO verovatnoća, sem sa srodnicima prvog i drugog stepena srodstva, koreliše i sa brojem obolelih srodnika u široj porodici. U odnosu na anatomsku lokalizaciju, BRCAPRO verovatnoća koreliše sa brojem srodnika obolelih od karcinoma dojke, ali ne i od karcinoma jajnika. Zaključak: Učestalost mutacija u genima BRCA1/2 kod ispitanika pod rizikom za nastanak naslednog karcinoma dojke i/ili jajnika u Srbiji slična je učestalostima dobijenim u drugim populacijama. Osim već poznatih mutacija u genima BRCA1/2 pokazane su i nove porodično-specifične mutacije. Nisu detektovane mutacije koje bi se mogle okarakterisati kao osnivačke mutacije za našu populaciju. BRCAPRO program se pokazao kao koristan pri odabiru osoba za BRCA testiranje, ali pri odabiru kandidata za testiranje, usled nedostataka BRCAPRO programa, treba analizirati i rodoslov. Podizanje granice BRCAPRO verovatnoće prilikom odabira ispitanika za BRCA testiranje sa 10% na 40% moglo bi da poveća efikasnost BRCA testiranja." ]
[ "Background: Protein products of BRCA1 and BRCA2 genes are included in important cellular processes, such as cell cycle control and DNA repair. Mutations in one of these genes is a step thowards losing control over genomic stability and cell division, and individuals who inherit mutations in these genes develop breast and/or ovarian cancer more frequently. BRCA genes are large, mutations are scattered throughout whole genes without clustering, which makes mutation detection even more difficult. The spectrum of BRCA mutations is different for each ethnic group. Mutations that are highly frequent in some populations are rare in other populations. At the same time, large proportion of BRCA mutations have been detected only once, and it can be said that majority of families under risk have their own family mutation. Mutation 5382insC in BRCA1 exon 20 is characteristic for Slavic populations and its frequency decreases from east to west of Europe. About 6-7% of all breast cancer cases and about 10% of all ovarian cancer cases are hereditary, associated to germ line mutations in BRCA1 and BRCA2 genes. BRCA mutation carriers have 5 to 8 times higher lifetime risk for breast cancer and 10 to 20 times higher lifetime risk for ovarian cancer. Aim of this study was to identify the most frequent BRCA1/2 mutations in our population and to find out which of them may be regarded as founder mutations, in order to make BRCA testing faster, easier and less expensive. In order to achieve this goal, it was necessary to identify types and frequencies of BRCA1/2 mutations in individuals with positive family history of breast and/or ovarian cancer in Serbia. In addition, it was necessary to identify the frequency of BRCA1 mutation 5382insC in breast cancer consecutive sample, as possible founder mutation in our population. In analysed group of individuals tested for the presence of BRCA1/2 mutations the efficiency of BRCAPRO software prediction was assessed. Material and methods: Eighty five peripheral blood samples from high risk individuals for breast and/or ovarian cancer from 69 families was analyzed. In addition, consecutive sample that included 257 blood samples from breast cancer patients was analyzed. The presence of BRCA gene mutations was analyzed by automatic sequencing, while the presence of 5382insC mutation in BRCA1 gene was determined by alel-specific PCR. For statistic analyses, non-parametric tests were used: χ2 test and Fisher test. Results: The frequency of deleterious mutations in sample of families with hereditary breast and/or ovarian cancer is 10.59% (9/85). All detected deleterious mutations are frameshift mutations. Three novel family-specific mutations have been detected (one in BRCA1 and two in BRCA2 gene). The frequency of BRCA1 mutation 5382insC in breast cancer consecutive sample is 0.39% (1/257). By raising the treshold of BRCAPRO probability to 40% it is possible to observe the difference between benign polymorphisms and deleterious mutations. Subgrouping of unclassified variants according to the value of BRCAPRO probability was not possible. BRCAPRO probability, in addition to the first and second degree relatives, correlates also with the number of more distant relatives who developed cancer. In relation to anatomic localisation, BRCAPRO probability correlates with the number of relatives who developed breast cancer, but not with the number of those who developed ovarian cancer. Conclusion: The frequency of BRCA1/2 mutations in probands under rsk for hereditary breast and/or ovarian cancer in Serbia is similar to those determined in other populations. In addition to already known BRCA1/2 mutations, new family-specific mutations have been detected. Mutation that could be qualified as founder mutations for our population have not been detected. BRCAPRO software has been shown to be useful in selection of probands for BRCA testing, but in this selection, due to BRCAPRO limitations, the pedigree should be analysed too. Raising the treshold for BRCAPRO probability in proband selection for BRCA testing from 10% to 40% could raise the efficiency of BRCA testing." ]
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Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "geni BRCA", "kontrola ćelijskog ciklusa", "popravka oštećenja DNK", "kancerogeneza", "karcinom dojke", "karcionom jajnika", "nasledni karcinom dojke i/ili jajnika", "genetičko testiranje" ]
[ "BRCA genes", "cell cycle control", "DNA repair", "carcinogenesis", "breast" ]
[ "Učestalost i tipovi mutacija u genima BRCA u porodicama sa pozitivnom istorijom za karcinom dojke i/ili jajnika u Srbiji", "Frequency and types of mutations in BRCA genes in families with positive history of breast/ovarian cancer in Serbia" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Стаменковић-Радак, Марина; Радуловић, Синиша; Бранковић-Магић, Мирјана; Добричић, Јелена Д.; Учесталост и типови мутација у генима БРЦA у породицама са позитивном историјом за карцином дојке и/или јајника у Србији; Учесталост и типови мутација у генима БРЦA у породицама са позитивном историјом за карцином дојке и/или јајника у Србији;
[ "https://nardus.mpn.gov.rs/bitstream/id/1968/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1968/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2054" ]
10.2298/bg20120523dobricic
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/11311
123456789-11311.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:19984/bdef:Content/download
no
Uticaj fiskalne politike na budžetsku, spoljnu i cenovnu ravnotežu u Republici Srbiji
The impact of fiscal policy on budgetary, external and price equilibria in the Republic of Serbia
[ "Arsić, Milojko" ]
[ "Mladenović, Zorica", "Urošević, Branko", "Nojković, Aleksandra", "Radulović, Branko" ]
Andrić, Vladimir
2019-09-09T13:52:52
[ "2019-09-09T13:52:52", "2020-07-03T08:31:51" ]
2019-03-15
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6862", "https://nardus.mpn.gov.rs/handle/123456789/11311", "https://fedorabg.bg.ac.rs/fedora/get/o:19984/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=512558434" ]
[ "U disertaciji se analizira uticaj fiskalne politike na budžetsku, spoljnu i cenovnu ravnotežu u Republici Srbiji nakon 2000. godine. Rezultati iz I poglavlja teze upućuju na neodrživost fiskalne pozicije Republike Srbije u analiziranom periodu, pri čemu je globalna finansijska kriza, zajedno sa izbornim političkim ciklusom, delovala u pravcu produbljivanja fiskalne neravnoteže, dok su aranžmani sa MMF-om delovali u pravcu stabilizacije javnih finansija Republike Srbije. Budžetska neravnoteža nakon 2000. godine je formirana kao posledica nesinhronizovanih diskrecionih mera na obe strane budžeta, što je u skladu sa hipotezom o institucionalnoj separaciji. Određeni empirijski dokazi iz II poglavlja, međutim, ukazuju da se putem kontrole javnih prihoda može uticati na dinamiku diskrecionih primarnih javnih rashoda, što je u skladu sa Fridmanovom oporezuj-troši hipotezom. Rezultati iz II poglavlja, takođe, odbacuju hipotezu o Rikardijanskoj ekvivalenciji, i upućuju da je neto efekat pozitivnih fiskalnih inovacija povezan sa: 1) aprecijacijom realnog i nominalnog efektivnog deviznog kursa; 2) smanjenjem privatne štednje domaćinstava; i 3) rastom bruto investicija. Opisani transmisioni mehanizmi deluju, zatim, u pravcu pogoršanja spoljnotrgovinske pozicije Republike Srbije. Konačno, rezultati iz III poglavlja teze pokazuju da fiskalna reakcija javnog duga na rast u primarnom fiskalnom bilansu postaje statistički značajna, u proseku, tek dve godine od početka sprovođenja programa fiskalne konsolidacije, što je u skladu sa teorijskim postavkama rikardijanskog, tj., monetarno-dominantnog, režima formiranja cena. Pojava statistički značajne negativne autokorelacije u stohastičkim procesima za primarni i strukturni primarni fiskalni bilans nakon isteka perioda od 3 godine upućuje, međutim, na potencijalnu relevantnost nerikardijanskog, tj., fiskalno-dominantnog, režima formiranja cena u slučaju Republike Srbije nakon 2000. godine." ]
[ "This thesis explores the impact of fiscal policy on budgetary, external and price equilibria in the Republic of Serbia after the year 2000. The results from the I thesis chapter point to unsustainable fiscal practices, with a particular emphasis on the destabilising effects of the Great Recession and the electoral political cycle, and the stabilising effects of the “stand-by” arrangements with the IMF. Budgetary disequilibrium in Serbia emerged as a consequence of ad-hoc discretionary measures on both sides of the budget, which is in accordance with the institutional separation hypothesis between government revenues and expenditures. Certain econometric estimates from the II thesis chapter, however, imply how policy makers can curtail primary government expenditures via government revenues control, giving support to Friedman’s tax-spend hypothesis. Other results from the II thesis chapter, which are concerned with the impact of fiscal policy on external equilibrium, reject the Ricardian equivalence hypothesis, and show how the net effect of unanticipated positive budgetary changes is connected with: 1) real and nominal exchange rate appreciation; 2) lower private saving; 3) higher gross fixed capital formation. Aforementioned transmission mechanisms lead, consequently, to the contraction of net exports. Finally, the III thesis chapter shows how fiscal reaction of public debt to an increase in primary fiscal balance becomes statistically significant, on average, after 2 years of fiscal consolidation, an empirical result consistent with the Ricardian, i.e., monetary-dominant, price formation regime. The occurrence of statistically significant negative autocorrelation in the stochastic processes for primary fiscal balance and structural primary fiscal balance after a 3 year period points, however, to potential relevance of nonricardian, i.e., fiscally dominant, price formation regime in the Republic of Serbia after the year 2000." ]
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sr
Универзитет у Београду, Економски факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc/4.0/" ]
Универзитет у Београду
[ "fiskalna održivost", "blizanački deficiti", "fiskalna teorija cena", "Srbija" ]
[ "fiscal sustainability", "twin deficits", "fiscal theory of the price level", "Serbia" ]
[ "Uticaj fiskalne politike na budžetsku, spoljnu i cenovnu ravnotežu u Republici Srbiji" ]
[ "The impact of fiscal policy on budgetary, external and price equilibria in the Republic of Serbia" ]
doctoralThesis
[ "BY-NC" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/4806/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/4807/IzvestajKomisije20762.pdf", "http://nardus.mpn.gov.rs/bitstream/id/4806/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/4807/IzvestajKomisije20762.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_11311" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/11508
123456789-11508.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:20271/bdef:Content/download
no
Analiza govorno-jezičkog, socio-emocionalnog i kognitivnog razvoja bolesnika sa heterozigotnom mikrodelecijom regiona q11.2 na hromozomu 22
Analysis of speech and language, social-emotional and cognitive development of patients with heterozygous microdeletion of the region q11.2 of chromosome 22.
[ "Drakulić, Danijela" ]
[ "Stevanović, Milena", "Čuturilo, Goran", "Zeljić, Katarina", "Vuković, Mile" ]
Rakonjac, Marijana
2019-11-15T10:03:57
[ "2019-11-15T10:03:57", "2020-07-03T08:07:51" ]
2019-07-01
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6920", "https://nardus.mpn.gov.rs/handle/123456789/11508", "https://fedorabg.bg.ac.rs/fedora/get/o:20271/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51645711" ]
[ "Sažetak: Sindrom delecije 22q11.2 (22q11.2DS) karakteriše prisustvo heterozigotne mikrodelecije regiona q11.2 na hromozomu 22. Pokazano je da deca sa 22q11.2DS često kasne na polju govorno-jezičkog, socio-emocionalnog i kognitivnog razvoja. Kako u literaturi nema podataka o ovim sposobnostima kod bolesnika sa 22q11.2DS govornika južno-slovenskih jezika, u okviru ove doktorske disertacije analizirane su govorno-jezičke, kognitivne i socio-emocionalne sposobnosti bolesnika sa 22q11.2DS izvornih govornika srpskog jezika. Dijagnoza 22q11.2DS postavljena je na osnovu prisustva najmanje dve od pet najčešćih fenotipskih karakteristika 22q11.2DS (urođene srčane malformacije konotrunkalnog tipa (KSM), facijalna dismorfija, aplazija/hipoplazija timusa, rascep nepca, hipokalcemije). Za detekciju mikrodelecije 22q11.2 primenjene su fluorescentna in situ hibridizacija (FISH) i metoda višestrukog umnožavanja proba koje je zavisno od ligacije (MLPA). Na osnovu rezultata FISH i/ili MLPA metoda, bolesnici sa kliničkom dijagnozom 22q11.2DS svrstani su u eksperimentalne grupe E1 (kod kojih je detektovana mikrodelecija 22q11.2) i E2 (kod kojih mikrodelecija 22q11.2 nije detektovana). Familijarna forma 22q11.2DS utvrđena je kod četiri porodice. Kako su kod svih bolesnika grupa E1 i E2 detektovane KSM i kako one mogu da utiču na razvoj govorno-jezičkih, kognitivnih i socio-emocionalnih sposobnosti istraživanjem su bila obuhvaćena i bolesnici sa nesindromskim KSM kod kojih mikrodelecija 22q11.2 nije detektovana FISH metodom (grupa E3). Govorno-jezičke, kognitivne i socioemocionalne sposobnosti bolesnika grupa E1, E2 i E3 su poređene sa ovim sposobnostima vršnjaka urednog govorno-jezičkog, socio-emocionalnog i kognitivnog razvoja, dobrog zdravstvenog stanja bez prisutnih hroničnih bolesti (grupa K). Rezultati istraživanja su pokazali da bolesnici grupe E1 imaju lošije govorno-jezičke sposobnosti u poređenju sa bolesnicima grupa E2 i E3 i ispitanicima grupe K. Niži nivo psihofizioloških sposobnosti detektovan je kod bolesnika grupe E1 u poređenju sa bolesnicima grupa E2 i E3 i ispitanicima grupe K..." ]
[ "22q11.2 deletion syndrome (22q11.2DS) is caused by a heterozygous microdeletion of region q11.2 of chromosome 22. It has been shown that speech and language, cognitive and socio-emotional impairments are very common in children with 22q11.2DS. To the best of our knowledge, there are no published data about these abilities in children with 22q11.2DS, native speakers of South-Slavic languages. Therefore, the main goal of this Doctoral Dissertation is to analyze speech and language, cognitive and socio-emotional abilities of children with 22q11.2DS, monolingual native speakers of the Serbian language. Enrollment of patients was based on the presence of at least two out of the five major characteristics of 22q11.2DS (congenital heart malformations (CHM), facial dysmorphism, thymic aplasia/hypoplasia, palatal clefts and hypocalcemia). Fluorescent in situ hybridization (FISH) and multiplex ligation-dependent probe amplification (MLPA) have been applied in order to detect 22q11.2 microdeletion. Based on FISH/MLPA results, patients with phenotypic features of 22q11.2DS were divided into experimental groups E1 (with 22q11.2 microdeletion) and E2 (without the 22q11.2 microdeletion). A familial form of 22q11.2DS was detected in four families. Patients with non-syndromic CHM (group E3) were included in the study since literature data implies that these children may exhibit a speech and language, cognitive and socioemotional impairments. Applying FISH, 22q11.2 microdeletion was not detected in these patients. Speech-language, cognitive and socio-emotional abilities of patients from the groups E1, E2 and E3 were compared to their age peers with proper speechlanguage, cognitive and socio-emotional development, and good general health condition without chronic diseases (group K). Obtained results revealed that patients from group E1 have less developed speech and language skills and psychophysiological abilities compared to patients from group E2 and E3 and children from group K. Also, cognitive abilities of 38.9% of patients from group E1, 18.8% of patients from group E2 and 25% of patients from group E3 did not reach levels expected for their calendar age..." ]
application/pdf
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Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/178027/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "sindrom delecije 22q11.2", "mikrodelecija 22q11.2", "govorno-jezičke sposobnosti", "kognitivne sposobnosti", "socio-emocionalne sposobnosti" ]
[ "22q11.2 deletion syndrome", "22q11.2 microdeletion", "speech and language abilities", "cognitive abilities", "social-emotional skills" ]
[ "Analiza govorno-jezičkog, socio-emocionalnog i kognitivnog razvoja bolesnika sa heterozigotnom mikrodelecijom regiona q11.2 na hromozomu 22" ]
[ "Analysis of speech and language, social-emotional and cognitive development of patients with heterozygous microdeletion of the region q11.2 of chromosome 22." ]
doctoralThesis
[ "BY-NC-ND" ]
[ "http://nardus.mpn.gov.rs/bitstream/id/1712/IzvestajKomisije21115.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1711/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1711/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1712/IzvestajKomisije21115.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_11508" ]
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https://nardus.mpn.gov.rs/handle/123456789/2035
123456789-2035.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:3046/bdef:Content/download
no
Neuroinflamatorni procesi mozga u amiotrofičnoj lateralnoj sklerozi : studija na modelu hSOD1 G93A pacova
Neuroinflammatory processes in the amyotrophic latera sclerosis brain : study of hSOD1 GV3A pacova
[ "Anđus, Pavle" ]
[ "Bačić, Goran", "Božić, Biljana", "Stević, Zorica" ]
Bataveljić, Danijela B.
2016-01-05T11:45:30
[ "2016-01-05T11:45:30", "2020-07-03T08:08:22" ]
2012-11-06
[ "https://nardus.mpn.gov.rs/handle/123456789/2035", "http://eteze.bg.ac.rs/application/showtheses?thesesId=51", "https://fedorabg.bg.ac.rs/fedora/get/o:3046/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=41902607" ]
[ "Amiotrofična lateralna skleroza (ALS) je neurodegenerativno oboljenje koje pogađa motorne neurone u kičmenoj moždini i višim moždanim centrima. Bolest karakteriše vrlo brza progresija i kratak životni vek od pojave simptoma. Predloženo je više mehanizama koji opisuju patogenezu bolesti, ali je uzrok ALS i dalje nepoznat. Cilj ove studije je da okarakteriše promene u višim moždanim centrima u modelu hSOD1G93A pacova i pokuša da dodatno razjasni mehanizme bolesti. Primenom tehnike magnetno-rezonantnog oslikavanja lokalizovana su neurodegenerativna žarišta u mozgu hSOD1G93A pacova. Istom metodom je in vivo praćena infiltracija T-limfocita pomoću posebno dizajniranih antitela obeleženih ultra malim paramagnetnim partikulama gvožđe oksida. Pokazano je da se CD4+ T-limfociti infiltriraju u tkivo oko moždanih komora, a CD8+ T-limfociti u moždano stablo hSOD1G93A pacova. Praćenje stanja krvno-moždane barijere pomoću Gd-DTPA kontrastnog agensa je ukazalo na njenu narušenost u moždanim regionima gde je uočena infiltracija T-ćelija. Prisustvo aktivirane mikroglije i reaktivnih astrocita je uočeno u moždanom stablu hSOD1G93A pacova. Pored toga, reaktivna mikroglija je uočena u moždanom tkivu oko lateralnih komora i hipokampusu. Nastavci aktivirane mikroglije ostvaruju kontakt sa telom neurona što ukazuje na mogućnost njihove interakcije. Primenom Western blot metode je pokazano da je ekspresija akvaporina-4 (AQP4) povećana, a ulaznog kalijumovog ispravljača (Kir4.1) smanjena u moždanom stablu i moždanoj kori hSOD1G93A pacova. Imunohistohemija na zamrznutim moždanim presecima je pokazala povećanu AQP4 i smanjenu Kir4.1 imunoreaktivnost u nc. facialis, nc. trigeminus i motornoj kori hSOD1G93A pacova. Kada su ispitivani kortikalni astrociti u kulturi, uočena je povećana AQP4 i smnjena Kir4.1 imunoreaktivnost u ALS. Proučavanje funkcionalnih osobina Kir kanala je ukazalo na smanjenu gustinu struja i smanjenu specifičnu membransku provodljivost u ALS astrocitima u kulturi. Pored toga, blokiranje ovih struja dodavanjem 1 mM CsCl ili 100 μM BaCl2 u vanćelijski rastvor je pokazalo da su Cs+-senzitivne odnosno Ba2+-senzitivne struje značajno manje u ALS astrocitima. U ovoj studiji su lokalizovana žarišta neurodegenerativnih i neuroinflamatornih promena u mozgu hSOD1G93A pacova, uočeno je oštećenje krvno-moždane barijere, a posebno su istaknute promene u ekspresiji i funkciji astrocitnih proteina koji doprinose stabilnosti krvno-moždane barijere. Dobijeni podaci su pružili dodatna objašnjenja koja doprinose razumevanju mehanizama ALS." ]
[ "Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder affecting lower and upper motor neurons. The disease shows fast progression and short lifetime following the disease onset. Several mechanisms contributing to ALS pathogenesis have been proposed, but the cause of disease remains unclear. The aim of this study was to characterize changes in the upper CNS regions of the hSOD1G93A rat model and to try to clarify disease mechanisms. Foci of neurodegeneration and neuroinflammation were localized in the brain of hSOD1G93A rat using magnetic resonance imaging. The same method was applied for in vivo monitoring of T-lymphocytes infiltration using specifically designed antibodies conjugated with ultrasmall superparamagnetic iron oxide particles. It was shown that CD4+ T-lymphocytes were infiltrated into the brain tissue around lateral ventricles while CD8+ T-lymphocytes were infiltrated into the brainstem of the hSOD1G93A rat. Using Gd-DTPA contrast, it has been shown that the blood-brain barrier was compromised in the brain regions invaded with T-cells. Activated microglia and reactive astrocytes were observed in the brainstem of the hSOD1G93A rat. In addition, reactive microglia was shown in the brain tissue around lateral ventricles and in the hippocampus. Processes of activated microglia were in contact with neuronal somata indicating possible interaction. Western blot results showed increased aquaporin-4 (AQP4) and reduced inwardly rectifying potassium channel (Kir4.1) expression in the brainstem and cortex of the hSOD1G93A rat. Immunohistochemistry on the frozen brain slices showed increased AQP4 and decreased Ki4.1 immunoreactivity in the nc. facialis, nc. trigeminus and motor cortex of the hSOD1G93A rat. Increased AQP4 and decreased Kir4.1 immunoreactivity were retained in cortical ALS astrocytes in culture. Examination of the functional properties of Kir channels revealed a decreased current density and diminished specific membrane conductance in ALS astrocytes in culture. Addition of 1 mM CsCl or 100 μM BaCl2 to the extracellular solution revealed significantly lower Cs+-sensitive and Ba2+-sensitive Kir currents in ALS. In this study we localized and characterized neurodegenerative nad neuroinflammatory changes in the brain of hSOD1G93A rat, confirmed the blood-brain barrier compromise and observed changes in the astrocytic proteins involved in the maintenance of the blood-brain barrier. Obtained data may contribute to further understanding of the mechanisms underlying ALS pathology." ]
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Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Amiotrofična lateralna skleroza", "neuroinflamacija", "krvno-moždana barijera", "akvaporin-4", "ulazni kalijumski ispravljač" ]
[ "Amyotrophic lateral sclerosis", "neuroinflammation", "blood-brain barrier", "aquaporin-4", "inwardly rectifying potassium channel" ]
[ "Neuroinflamatorni procesi mozga u amiotrofičnoj lateralnoj sklerozi : studija na modelu hSOD1 G93A pacova", "Neuroinflammatory processes in the amyotrophic latera sclerosis brain : study of hSOD1 GV3A pacova" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Aнђус, Павле; Божић, Биљана; Стевић, Зорица; Бачић, Горан; Батавељић, Данијела Б.; Неуроинфламаторни процеси мозга у амиотрофичној латералној склерози : студија на моделу хСОД1 Г93A пацова; Неуроинфламаторни процеси мозга у амиотрофичној латералној склерози : студија на моделу хСОД1 Г93A пацова;
[ "http://nardus.mpn.gov.rs/bitstream/id/1874/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1874/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2035" ]
10.2298/bg20121106bataveljic
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/5074
123456789-5074.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:10794/bdef:Content/download
no
Karakterizacija antimikrobnih supstanci prirodnih izolata Bacillus sp. za primenu u biološkoj kontroli fitopatogenih bakterija i gljiva
Characterization of antimicrobial substances from natural isolates of Bacillus sp. for application in biological control of plant pathogenic bacteria and fungi
[ "Stanković, Slaviša" ]
[ "Berić, Tanja", "Fira, Đorđe", "Lozo, Jelena", "Stević, Tatjana" ]
Dimkić, Ivica Z.
2016-04-03T18:25:59
[ "2016-04-03T18:25:59", "2020-07-03T08:07:30" ]
2015-04-04
[ "https://nardus.mpn.gov.rs/handle/123456789/5074", "http://eteze.bg.ac.rs/application/showtheses?thesesId=2682", "https://fedorabg.bg.ac.rs/fedora/get/o:10794/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=47636239" ]
[ "Bolesti biljaka izazvane infekcijama patogenim bakterijama i gljivama mogu dovesti do smanjenja kapaciteta biljnog rasta ili naneti mnogo ozbiljniju štetu dovodeći do smrti biljaka i značajnih gubitaka u proizvodnji hrane. Veliki broj istraživanja je u poslednje vreme posvećen proučavanju roda Bacillus kao producenta sekundarnih metabolita u kontroli različitih biljnih patogena. Analizom diverziteta gena odgovornih za produkciju neribozomalno sintetisanih lipopeptida kolekcije od 205 Bacillus sp. izolata utvrđeno je da je za najveći broj izolata (171) ustanovljen DNK fragment očekivane dužine, koji odgovara bacilomicin D sintetazi, dok su u sličnom broju izolata dobijeni fragmenti očekivanih dužina za kurstakinske (154) i surfaktinske operone (142), a prisustvo iturinskog operona ustanovljeno je kod 84, odnosno 59 izolata u slučaju gena za fengicin sintetazu. Prisustvo svih pet biosintetičkih operona je potvrđeno kod 11 izolata. Najveća produkcija antimikrobnih jedinjenja za pet izabranih izolata je pokazana u eksponencijalnoj ili ranoj stacionarnoj fazi rasta. Prikazan je jak antifungalni efekat lipopeptidnih ekstrakata prema više od 30 testiranih gljiva, sa najnižim zabeleženim minimalnim inhibitornim koncentracijama od 0,008 mg/ml protiv Fusarium semitectum, kao i izražen antibakterijski potencijal, naročito protiv fitopatogenih vrsta rodova Xanthomonas i Pseudomonas, sa najnižom minimalnom inhibitornom koncentracijom od 0,06 mg/ml. Proučavanje interakcija pojedinačnih lipopeptidnih ekstrakata u međusobnim, kao i u kombinacijama sa etarskim uljima, ukazalo je na postojanje sinergističkog efekta za neke kombinacije u in vitro, odnosno aditivnog efekta u in situ uslovima, na bakterijskim i fungalnim patogenima. Utvrđivanjem direktnog antagonističkog efekta lipopeptidnih jedinjenja in planta, ustanovljena je značajna redukcija nekroze tkiva listova Arabidopsis thaliana L. i Beta vulgaris L., tretiranih bakterijskim patogenima. Takođe, u in situ uslovima postignuta je značajna redukcija nekroze tkiva ploda jabuke izazvana fungalnom infekcijom..." ]
[ "Plant diseases caused by infection with pathogenic bacteria and fungi can lead to the reduction in the capacity of plant growth or can cause far more serious damage, leading to the death of plants and significant losses in food production. Numerous recent studies are devoted to the genus Bacillus as a producer of secondary metabolites in the control of different plant pathogens. In the analysis of diversity of genes responsible for the nonribosomal synthesized lipopeptides in collections of 205 Bacillus sp. isolates, for the largest number of isolates (171) the DNA PCR fragment of the expected length, corresponding to bacillomycin D synthases was obtained, while in a similar number of isolates fragments of the expected length for kurstakin (154) and surfactin operons (142) were obtained. The presence of iturin operon in the 84 or 59 isolates in the case of the gene for fengycin synthetase, was also found. The presence of all five biosynthetic operons in the 11 isolates was confirmed as well. The maximum production of antimicrobial compounds for five selected isolates in exponential or early stationary phase of growth was determined. Strong antifungal effect of lipopeptide extracts towards more than 30 fungi tested, with lowest recorded minimal inhibitory concentrations of 0,008 mg/ml against Fusarium semitectum, as well as expressed antibacterial potential, in particular against phytopathogenic species of the genera Pseudomonas and Xanthomonas, with the lowest minimum inhibitory concentration of 0,06 mg/ml was determined. In the analysis of the interaction of individual lipopeptide extracts mutually, as well as in combination with essential oils, the existence of a synergistic effect in vitro respectively additive effect in situ, on bacterial and fungal pathogens, for some combinations were shown. Establishing a direct antagonistic effect of lipopeptide compounds in planta, significant reduction in necrotic lesions of leaves of Arabidopsis thaliana L. and Beta vulgaris L. treated with bacterial pathogens was obtained. Also, a significant reduction in necrotic lesions of apple fruit caused by fungal infection in situ was determined..." ]
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Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173026/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Bacillus", "lipopeptidna jedinjenja", "biološka kontrola", "antimikrobna aktivnost", "sinergizam" ]
[ "Bacillus", "lipopeptides", "biological control", "antimicrobial activity", "synergism" ]
[ "Karakterizacija antimikrobnih supstanci prirodnih izolata Bacillus sp. za primenu u biološkoj kontroli fitopatogenih bakterija i gljiva", "Characterization of antimicrobial substances from natural isolates of Bacillus sp. for application in biological control of plant pathogenic bacteria and fungi" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Станковић, Славиша; Берић, Тања; Фира, Ђорђе; Лозо, Јелена; Стевић, Татјана; Димкић, Ивица З.; Карактеризација антимикробних супстанци природних изолата Бациллус сп. за примену у биолошкој контроли фитопатогених бактерија и гљива; Карактеризација антимикробних супстанци природних изолата Бациллус сп. за примену у биолошкој контроли фитопатогених бактерија и гљива;
[ "https://nardus.mpn.gov.rs/bitstream/id/1617/Disertacija683.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1618/Dimkic_Ivica_Z.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1617/Disertacija683.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1618/Dimkic_Ivica_Z.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_5074" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/2297
123456789-2297.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:5991/bdef:Content/download
no
Dinamička analiza posebnih klasa linearnih singularnih sistema sa kašnjenjem na konačnom i beskonačnom vremenskom intervalu
Dynamical analysis of particular classes of linear time-delay singular control systems defined over finite and infinite time interval
[ "Debeljković, Dragutin" ]
[ "Stojanović, Sreten", "Lazarević, Mihailo" ]
Aleksendrić, Marko S.
2016-01-05T12:01:45
[ "2016-01-05T12:01:45", "2020-07-03T08:40:52" ]
2012-10-17
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=347", "https://nardus.mpn.gov.rs/handle/123456789/2297", "https://fedorabg.bg.ac.rs/fedora/get/o:5991/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=514088099" ]
[ "U disertaciji su razmatrani problemi dinamicke analize posebnih klasa singularnih sistema sa cistim vremenskim kašnjenjem prisutnim u stanju sistema, kao i njihovo ponašanje na konacnom i beskonacnom vremenskom intervalu. Pružen je presek savremenih koncepata stabilnosti, prednosti jednih nad drugima i posebno su obraeni tzv neljapunovski koncepti: stabilnost na konacnom vremenskom intervalu i koncept prakticne stabilnosti. Nadograene su osnovne definicije stabilnosti. Isrpno je izložen hronološki sistematican pregled osnovnih koncepata stabilnosti, polazeci od ljapunovske metodologije, kao osnove na kojoj se zasniva dinamicka analiza sistema. Ukazano je na istorijski razvoj i nastanak ideja i rezultata u ovoj oblasti i na taj nacin su izvedene i smernice daljih istraživanja otvorenih problema. U disertaciji su sistemi tretirani sa stanovišta dva savremena pristupa: deskriptivnog i LMI, odnosno sa pozicija linearnih matricnih nejednakosti, koja se svodi na metode konveksne optimizacije. Izvedeni su i saopšteni novi rezultati. Izložen je prilaz koji se bazira na kvaziljapunovskim funkcijama za dobijanje uslova prakticne i stabilnosti na konacnom vremenskom intervalu posebne klase singularnih sistema sa cistim vremenskim kašnjenje, u stanju sistema. Pokazano je da, polazeci od pretpostavke da agregacione funkcije ne moraju da budu odreene po znaku i da njihovi izvodi duž trajektorija sistema ne moraju da budu negativno odrreene funkcije, uz pomoc deskriptivnog prilaza se mogu dobiti novi kriterijumi za ocenu neljapunovske stabilnosti. Kombinovanjem rezultata sa ljapunovskim prilazom, izvedeni su o uslovi atraktivne prakticne stabilnosti. Drugi doprinos je odreivanje dovoljnih uslova stabilnosti na konacnom vremenskom intervalu iste klase sistema pomocu savremenih LMI metoda. Dobijeni i prezentovani rezultati imaju prakticnu primenu u savremenoj teoriji i praksi upravljanja i mogu se primeniti na sve klase proucavanih sistema, pod uslovom da su dostupni verodostojni matematicki modeli. Verifikacija rezultata je izvedena kroz numericke primere" ]
[ "In this thesis the problems of dynamical analysis of particular class of singular control systems with time delays are considered, as well as their behavior on finite and infinite time intervals. Emphasis has been put on the peculiar properties of singular ad descriptor systems, concerning the existence and uniqueness of the solutions, the problems of impulsive behavior, consistent initial conditions and causality of the system itself. On overview of the modern stability frameworks has been presented, starting from the classical Lyapunov ideas and extending through so called non-lyapunov concepts: finite time stability and practical stability in particular. A historical overview of ideas, concepts and results has been presented and the key contributions have been highlighted through key papers from the modern literature. This dissertation follows two main lines of research: the descriptive approach and the LMI (linear matrix inequalities) methodology, the latter being known to reduce control tasks to convex optimization problems, thus making them easily solvable by numerical computation. New results are presented. A new approach, based on lyapunov-like functions, is used in order to establish new sufficient conditions of practical and finite time interval stability of a particular class of singular time delay systems. Another new result is based on the modern LMI approach and gives new sufficient conditions for finite time stability. The obtained results are numerically verified and have great practical value, as they are easy to compute and less restrictive and conservative than their predecessors." ]
application/pdf
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Универзитет у Београду, Машински факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by/4.0/" ]
Универзитет у Београду
[ "Singularni sistemi", "deskriptivni sistemi", "stabilnost na konacnom vremenskom intervalu", "atraktivna prakticna stabilnost", "vremenski diskretni i kontinualni sistemi sa cistim vremenskim kašnjenjem", "prakticna stabilnost" ]
[ "Singular systems", "descriptor systems", "finite time stability", "attractive practical stability", "discrete nad continuous time-delay singular systems", "practical stability" ]
[ "Dinamička analiza posebnih klasa linearnih singularnih sistema sa kašnjenjem na konačnom i beskonačnom vremenskom intervalu", "Dynamical analysis of particular classes of linear time-delay singular control systems defined over finite and infinite time interval" ]
[ "" ]
doctoralThesis
[ "BY" ]
Дебељковић, Драгутин; Лазаревић, Михаило; Стојановић, Сретен; Aлексендрић, Марко С.; Динамичка анализа посебних класа линеарних сингуларних система са кашњењем на коначном и бесконачном временском интервалу; Динамичка анализа посебних класа линеарних сингуларних система са кашњењем на коначном и бесконачном временском интервалу;
[ "https://nardus.mpn.gov.rs/bitstream/id/6956/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/6956/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2297" ]
10.2298/bg20121017aleksendric
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https://nardus.mpn.gov.rs/handle/123456789/11827
123456789-11827.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:20876/bdef:Content/download
no
Derivati ramnolipida i 4-aminohinolina kao inhibitori virulencije kod vrsta Pseudomonas aeruginosa i Serratia marcescens
Derivatives of rhamnolipids and 4-aminoquinoline as Pseudomonas aeruginosa and Serratia marcescens virulence inhibitors
[ "Stanković, Slaviša" ]
[ "Stanković, Slaviša", "Šenerović, Lidija", "Šenerović, Lidija", "Opsenica, Dejan" ]
Aleksić, Ivana
2020-02-07T10:29:24
[ "2020-02-07T10:29:24", "2020-07-03T08:08:14" ]
2019-11-08
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=7181", "https://nardus.mpn.gov.rs/handle/123456789/11827", "https://fedorabg.bg.ac.rs/fedora/get/o:20876/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025237170" ]
[ "Rezistencija bakterija na antibiotike je rastući problem globalnih razmera tako da su pronalaženje i uvođenje novih terapijskih opcija u borbu protiv bakterijskih infekcija postali prioriteti i fundamentalnih i primenjenih istraživanja. U potrazi za jedinjenjima prirodnog porekla sa anti-virulentnom aktivnošću izdvojen je nepatogeni soj iz rizosfere biljke dobračice (Glechoma hederacea), Lysinibacillus sp. BV152.1, čiji su metaboliti inhibirali formiranje biofilmova P. aeruginosa PAO1. Hemijske analize su pokazale da aktivnu komponentu etil acetatnog ekstrakta čini smeša ramnolipida. Uporednom analizom anti-biofilm aktivnosti utvrđeno je da su di-ramnolipidi soja Lysinibacillus sp. BV152.1 bolji inhibitori procesa formiranja biofilmova (adhezije i sazrevanja) kod P. aeruginosa PAO1 od komercijalno dostupnih ramnolipida, poreklom iz P. aeruginosa. Amidnom derivatizacijom di-ramnolipida poboljšana je njihova anti-biofilm aktivnost, gde je najaktivniji derivat di-ramnolipida iz Lysinibacillus sp. BV152.1 imao morfolinsku grupu i inhibirao je formiranje biofilmova P. aeruginosa PAO1 za 80% u koncentraciji od 100 μg/ml, a isti derivat u koncentraciji od 50 μg/ml inhibirao je formiranje biofilmova S. marcescens za 88%. Hinolini, jedinjenja sa širokim spektrom bioloških aktivnosti, a takođe i autoinduceri PQS signalnog puta međubakterijske komunikacije P. aeruginosa predstavljaju dobru osnovu za razvoj anti-virulentnih jedinjenja. Derivati aminohinolina sintetisani u ovoj studiji nisu pokazali značajnu antibakterijsku aktivnost prema Gram negativnim patogenima. Najveću inhibitornu aktivnost na formiranje biofilmova kod P. aeruginosa i S. marcescens imala su jedinjenja koja su sadržala atom hlora ili CF3 grupu na poziciji C(7) i alifatični lanac sa 12 C atoma na poziciji C(4) (jedinjenja 5 i 10), sa BFIC50 koncentracijama od 69 μM odnosno 63 μM. Ova jedinjenja su prvi derivati hinolina za koje je utvrđena sposobnost da inhibiraju biofilmove S. marcescens. Detaljnom analizom odnosa strukture i aktivnosti jedinjenja 5 i 10 je pokazano da dužina alifatičnog lanca i lipofilnost jedinjenja imaju najveći uticaj na formiranja biofilomova kod S. marcescens. Jedinjenje 10 je izdvojeno kao najpotentniji inhibitor proizvodnje piocijanina kod P. aeruginosa hinolinske prirode opisan do sada..." ]
[ "Bacterial resistance to antibiotics is a growing problem on a global scale, so finding and introducing new therapeutic options to combat bacterial infections has become a priority in both fundamental and applied research. In a search for structurally new compounds with anti-virulent activity, novel non-pathogenic strain was isolated from the rhizosphere of a Glechoma hederacea plant, named BV152.1, whose metabolites inhibited the formation of P. aeruginosa PAO1 biofilms. Chemical analyzes have shown that the active compound of ethyl acetate extract is a mixture of rhamnolipids. Comparative analysis of their anti-biofilm activity revealed that the di-rhamnolipids from the strain Lysinibacillus sp. BV152.1 are better inhibitors of P. aeruginosa PAO1 biofilm adhesion and maturation, than commercially available rhamnolipids, originated from P. aeruginosa. The amide derivatization of di-rhamnolipids enhanced the anti-biofilm activity of these compounds, where the most active di-rhamnolipid derivative from Lysinibacillus sp. BV152.1 had a morpholine group and inhibited the formation of P. aeruginosa PAO1 biofilms by 80% at a concentration at 100 μg/ml. The same derivative at 50 μg/ml inhibited formation of S. marcescens biofilm by 88%. Quinolines, compounds with a wide range of biological activities, and autoinducers of the quorum sensing PQS signaling pathway of P. aeruginosa represent a good basis for the development of compounds with anti-virulent activity. The aminoquinoline derivatives synthesized in this study did not show significant antibacterial activity against Gram-negative pathogens, making them suitable chemical structures for the development of anti-virulent agents. The highest inhibitory activity of P. aeruginosa and S. marcescens biofilm formation showed compounds containing a chlorine atom (compound 5) or CF3 group (compound 10) at position C(7) and an aliphatic chain of 12 C atoms at position C(4) with BFIC50 concentrations of 69 μM and 63 μM, respectively. These compounds are the first quinoline derivatives identified to have the ability to inhibit S. marcescens biofilms formation. Detailed analysis of the structure and activity relationships of compounds 5 and 10 showed that the aliphatic chain length and lipophilicity of the compounds had the greatest influence on inhibition of biofilm formation in S. marcescens..." ]
application/pdf
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Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173048/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "P. aeruginosa", "S. marcescens", "Lysinibacillus sp.", "anti-viurulentna aktivnost", "ramnolipidi", "hinolini", "PQS", "PqsR" ]
[ "P. aeruginosa", "S. marcescens", "Lysinibacillus sp.", "anti-virulent activity", "rhamnolipids", "quinolines", "PQS", "PqsR" ]
[ "Derivati ramnolipida i 4-aminohinolina kao inhibitori virulencije kod vrsta Pseudomonas aeruginosa i Serratia marcescens" ]
[ "Derivatives of rhamnolipids and 4-aminoquinoline as Pseudomonas aeruginosa and Serratia marcescens virulence inhibitors" ]
doctoralThesis
[ "BY-NC-ND" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1835/IzvestajKomisije21837.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1834/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1834/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1835/IzvestajKomisije21837.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_11827" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/11506
123456789-11506.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:20262/bdef:Content/download
no
Uticaj dugotrajne dijetalne restrikcije na insulinski signalni put u mozgu pacova tokom starenja
The effect of long-term dietary restriction on insulin signaling pathway in rat brain during aging
[ "Kanazir, Selma" ]
[ "Mladenović-Đorđević, Aleksandra", "Smiljanić, Kosara" ]
Todorović, Smilja
2019-11-15T10:03:56
[ "2019-11-15T10:03:56", "2020-07-03T08:07:48" ]
2019-07-01
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6913", "https://nardus.mpn.gov.rs/handle/123456789/11506", "https://fedorabg.bg.ac.rs/fedora/get/o:20262/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025218994" ]
[ "Starenje je normalan, fiziološki proces koji obuhvata sve organe i organske sisteme i tokom koga se organizam suočava sa nizom strukturnih i funkcionalnih promena. Starenje mozga je izrazito kompleksan proces, predstavljen nizom sukcesivnih događaja koji postepeno rezultiraju gubitkom kognitivnih i motornih funkcija. Postoji niz farmakoloških i sredinskih faktora koji su u stanju da odlože i/ili uspore mnoge od starosno-zavisnih procesa, a dijetalna restrikcija (DR) je jedna od najviše i najduže istraživanih. Mnogobrojni su eksperimentalni i epidemiološki podaci koji govore u prilog korisnih efekata restriktivnog režima ishrane, bez obzira da li se radi o svakodnevnoj, ili intermitentnoj dijeti, smanjenju količine hrane ili kalorija. Međutim, u poslednje vreme se sve više pojavljuju podaci koji dovode u pitanje univerzalnost korisnog dejstva dijete. Da bi ovakva intervencija postigla svoj optimalni efekat, neophodno je povesti računa o određenim specifičnostima prilikom uspostavljanja restriktivnog režima ishrane. Pre svega, pokazano je da stepen restrikcije igra bitnu ulogu u efektima koje ona proizvodi i da previše restriktivan režim ishrane može imati negativne posledice po organizam. Takođe, postoje indicije da nisu svi organski sistemi podjednako podložni procesima starenja, kao ni dejstvu dijete. Efekat dijete se može veoma razlikovati u zavisnosti od stepena i tipa restrikcije, pola i organa gde se efekat očekuje, kao i od starosti jedinke na koju se restriktivni režim primenjuje. Prvi korak u istraživanju korisnih efekata restrikcije hrane koja je iznosila 60% prosečnog dnevnog unosa hrane, bio je ispitati efekat različitih dijetalnih režima, odnosno efekte DR koja je različito trajala i koja je započeta u različitim životnim dobima kod mužjaka Wistar soja pacova. Praćen je čitav niz parametara u aktivnosti životinja i detektovane su značajne razlike u njihovim fizičkim i kognitivnim preformansama, kako tokom starenja, tako i pod uticajem različitih režima ishrane. Dugotrajna dijetalna restrikcija otpočeta u adultnom dobu je dovela do poboljšanja u izvođenju motoričkih i kognitivnih testova i do sveukupnog poboljšanog stanja organizma, što se ogledalo u smanjenoj krhkosti ovih životinja..." ]
[ "Aging is a normal, physiological process that involves all organs and organic systems, and during which the organism faces a number of structural and functional changes. Brain aging is an extremely complex process, represented by a series of successive events that gradually result in loss of cognitive and motor functions. There are a number of pharmacological and environmental factors that are able to postpone and / or slow down many of the age-dependent processes, and dietary restriction (DR) is one of the most investigated and most widely used experimental intervention in aging research. There are numerous experimental and epidemiological data that support the beneficial effects of a restrictive diet, regardless of the type: a daily reduction or intermittent diet, reduction in food or in calories, all of those approaches seem beneficial. Nevertheless, there is a body of data that challenges current premise about comprehensive DR usefulness. In order for DR to achieve its optimal effect, it is necessary to take certain specificities into account while establishing a restrictive dietary regime. First of all, it has been shown that the percentage of restriction plays an important role in the effects it induces, and that a very restrictive diet may have negative consequences for the organism. In addition, not all the body systems are equally susceptible to aging processes, nor to the beneficial effects of DR. The effect of the restrictive diet can vary greatly, depending on the degree and type of restriction, gender and organs examined, as well as the age of the individual to which the restrictive regimen is applied. In order to investigate the effect of 60% DR on the energy homeostasis and insulin signaling in the brain, we first examined the effects of three different types of dietary restriction that varied in length and onset, on rat behavior during aging. The experiments were performed on 12-, 18- and 24-month-old male Wistar rats exposed to AL or DR type of feeding (60% of AL daily intake). A variety of parameters were monitored in the activities of animals and significant differences in their physical and cognitive performance were detected, both during aging and under the influence of different dietary regimes..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173056/RS//", "info:eu-repo/grantAgreement/MESTD/MPN2006-2010/143004/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]
Универзитет у Београду
[ "mozak", "dijetalna restrikcija", "starenje", "krhkost", "motorička aktivnost", "habituacija", "prostorna memorija", "energetska homeostaza", "metabolizam glukoze", "insulin" ]
[ "dietary restriction", "aging", "frailty", "motor activity", "habituation", "spatial memory", "glucose metabolism", "energy homeostasis", "insulin", "brain" ]
[ "Uticaj dugotrajne dijetalne restrikcije na insulinski signalni put u mozgu pacova tokom starenja" ]
[ "The effect of long-term dietary restriction on insulin signaling pathway in rat brain during aging" ]
doctoralThesis
[ "BY-NC-SA" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1694/IzvestajKomisije21108.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1694/IzvestajKomisije21108.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1693/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1693/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_11506" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/10054
123456789-10054.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:18541/bdef:Content/download
no
Biološki potencijal i analiza metabolita jestivih i lekovitih gljiva iz rodova Agrocybe, Laetiporus, Pleurotus i Polyporus sa teritorije Srbije
Biological potential and metabolite analysis of edible and medicinal fungi from genera Agrocybe, Laetiporus, Pleurotus i Polyporus from the territory of Serbia
[ "Glamočlija, Jasmina" ]
[ "Vukojević, Jelena", "Soković, Marina", "Stanković, Slaviša", "Robajac, Dragana" ]
Petrović, Jovana D.
2018-11-07T15:53:14
[ "2018-11-07T15:53:14", "2020-07-03T08:07:05" ]
2018-07-12
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6106", "https://nardus.mpn.gov.rs/handle/123456789/10054", "https://fedorabg.bg.ac.rs/fedora/get/o:18541/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50644495" ]
[ "krupna plodonosna tela, dovoljno velika da se vide golim okom i uberu rukom. U ovoj doktorskoj disertaciji makromicete iz rodova: Agrocybe, Laetiporus, Pleurotus i Polyporus, sakupljene na teritoriji Srbije, detaljno su analizirane. Ispitivan je sadržaj makronutrijenata i odabranih metabolita u makromicetama i utvrđen je njihov biološki potencijal. Identifikacija i određivanje sadržaja nutrijenata (ukupnih ugljenih hidrata, proteina, masti i pepela), šećera, tokoferola, masnih, fenolnih i organskih kiselina izvedeno je putem HPLC-a sa različitim detektorima u zavisnosti od jedinjenja od interesa. Radi detaljne procene biološkog potencijala ekstrakata makromiceta, izvedeni su testovi in vitro antioksidativne, antimikrobne, anti-quorum sensing i citotoksične aktivnosti, a iz bazidiokarpa Laetiporus sulphureus 1 izolovan je i okarakterisan lektin i procenjen njegov antibakterijski potencijal. Rezultati analize makronutrijenata pokazali su da testirane makromicete imaju povoljan profil nutrijenata zbog čega se mogu preporučiti u ishrani. Karakteriše ih znatna količina ugljenih hidrata i proteina kao i nizak sadržaj masti. Analizom šećera identifikovane su: ramnoza, fruktoza, manitol, glukoza i trehaloza koja je bila i najzastupljeniji šećer sa 16,50 g/100 g suve mase kod Pleurotus ostreatus K i 32,60 g/100 g suve mase kod P. ostreatus S. U uzorcima testiranih vrsta makromiceta određen je sadržaj četiri izoforme tokoferola (α-, β-, γ- i δ- tokoferol), dok je analizom masnih kiselina potvrđeno prisustvo 27 masnih kiselina, među kojima je nutritivno poželjna linolna kiselina najviše zastupljena u uzorku Agrocybe aegerita 1 (78,40%). Među prostim organskim kiselinama utvrđeno je prisustvo: oksalne, kininske, jabučne, limunske i fumarne kiseline, dok su analizom organskih kiselina sa fenolnom grupom identifikovane protokatehinska, p-hidroksibenzoeva, p-kumarna i cinaminska kiselina. Izoloacijom i karakterizacijom lektina iz bazidiokarpa L. sulphureus 1 pokazano je da je lektin multimerne građe, pI vrednosti ~ 4,6. Rezultati antioksidativne aktivnosti (redukciona sposobnost, DPPH· aktivnost, β-karoten/linolna kiselina i TBARS test) pokazali su da su testirane makromicete moćni in vitro antioksidativni agensi više ili manje efikasni u neutralizaciji slobodnih radikala, inhibiciji lipidne peroksidacije i redukcionoj sposobnosti (EC50/ EC0.5 vrednosti su u opsegu 0,25 - 23,78 mg/mL). Etanolni i metanolni ekstrakti bazidiokarpa testiranih makromiceta pokazali su visok antimikrobni potencijal (u odnosu na vodeni ekstrakt) na testirane mikroorganizme, a utvrđeno je da pri testiranim koncentracijama lektin ne deluje na bakterije, što ide u prilog tvrdnji da supstance prirodnog porekla biološki efekat ostvaruju zahvaljujući aditivnim i sinergističkim interakcijama većeg broja prisutnih jedinjenja..." ]
[ "Macromycetes are fungi from subdivisions Ascomycotina and Basidiomycotina that are large enough to be seen with naked eye and to be picked by hand. In this thesis, selected species from the genera: Agrocybe, Laetiporus, Pleurotus and Polyporus were harvested in Serbia and chemically characterized. Their nutrients profile and selected metabolites as well as their biological potential was evaluated. Quantification of macronutrients (carbohydrates, proteins, fat and ash), selected sugars, tocopherols, fatty, phenolic and organic acids was determined by means of HPLC coupled with various detectors, depending on the type of desired compound. Regarding the evaluation of biological potential, detailed assesments of antioxidant, antimicrobial, anti-quorum sensing and cytotoxic activities were performed. A lectin was extracted from the fruiting body of Laetiporus sulphureus 1 as a compound of interest, and was evaluated for its antibacterial activity. Results of the macronutrient profile suggest that macromycetes are a rich source of carbohydrates and proteins, with low fat content which makes them excellent candidates for food. Sugar analysis revealed the presence of: rhamnose, fructose, mannitol, glucose and trehalose which was the most abundant sugar with 16,50 g/100 g dry weight in Pleurotus ostreatus K and 32,60 g/100 g dry weight in P. ostreatus S. Four isoforms of tocopherols (α-, β-, γ- and δ- tocopherol) were quantified in samples, while the fatty acid analysis revealed the presence of 27 fatty acids with linoleic acid as the most abundant polyunsatturated fatty acid in sample Agrocybe aegerita 1 (78,40%). Amongst simple organic acids, the following were detected in the samples: oxalic, quinic, malic, citric and fumaric acid. Phenolic group containing organic acids analysis indicated the presence of: protocatechuic, p-hydroxybenzoic, p-coumaric and cinnamic acid. Isolation and characterization of lectin from the L. sulphureus 1 basidiocarp revealed that lectin is a multimer with pI value of ~ 4,6. Results of antioxidant activity which was evaluated using four protocols (reducing power, DPPH· scavenging activity, β-caroten/linoleic acid and TBARS test) indicate that selected macromycetes are potent antioxidant agents that showed ability to neutralize free radicals, inhibit lipid peroxidation and reducing power (EC50/EC0.5 is in range of 0,25 – 23,78 mg/mL). Ethanolic and methanolic extracts of mushroom fruiting bodies revealed potent antimicrobial potential against the tested pathogenic microorganisms (compared to the aqueous extracts), while lectin didn't show antibacterial activity at the tested concentrations, which supports the claim that natural sourced substances achieve their full biological potential thanks to the additive and synergistic interactions of all the present compounds..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173032/RS//", "info:eu-repo/grantAgreement/MESTD/MPN2006-2010/143041/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc/4.0/" ]
Универзитет у Београду
[ "jestive makromicete", "nutrijenti", "odabrani metaboliti", "biološka aktivnost", "lektini" ]
[ "edible macromycetes", "nutrients", "selected metabolites", "biological activity", "lectins" ]
[ "Biološki potencijal i analiza metabolita jestivih i lekovitih gljiva iz rodova Agrocybe, Laetiporus, Pleurotus i Polyporus sa teritorije Srbije" ]
[ "Biological potential and metabolite analysis of edible and medicinal fungi from genera Agrocybe, Laetiporus, Pleurotus i Polyporus from the territory of Serbia" ]
doctoralThesis
[ "BY-NC" ]
Гламочлија, Јасмина; Вукојевић, Јелена; Соковић, Марина; Станковић, Славиша; Робајац, Драгана; Петровић, Јована Д.;
[ "https://nardus.mpn.gov.rs/bitstream/id/1529/IzvestajKomisije17878.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1528/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1528/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1529/IzvestajKomisije17878.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10054" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/10049
123456789-10049.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:18380/bdef:Content/download
no
Analiza ekspresije i uloge humanog gena SOX14 u neuralnoj diferencijaciji in vitro i regulaciji malignog fenotipa
Analysis of the expression and role of the human gene SOX14 in neural differentiation in vitro and in the regulation of malignant phenotype
[ "Popović, Jelena" ]
[ "Stevanović, Milena", "Savić Pavićević, Dušanka", "Popović, Jelena", "Stevanović, Milena" ]
Stanisavljević, Danijela D.
2018-11-07T15:53:11
[ "2018-11-07T15:53:11", "2020-07-03T08:06:58" ]
2018-05-04
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6046", "https://nardus.mpn.gov.rs/handle/123456789/10049", "https://fedorabg.bg.ac.rs/fedora/get/o:18380/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025186226" ]
[ "Visok stepen očuvanosti tokom evolucije i činjenica da do danas nije opisan nijedan klinički sindrom povezan sa mutacijama u genu SOX14, ukazuju na značajnu ulogu ovog gena tokom embrionalnog razvića. Iako je prošlo dvadeset godina od otkrića gena SOX14, funkcija ovog gena još uvek nije dovoljno istražena. U ovoj doktorskoj disertaciji analizirana je ekspresija i uloga gena SOX14 u procesu neuralne diferencijacije in vitro i regulaciji malignog fenotipa ćelija poreklom od karcinoma grlića materice. Tokom neuralnog razvića kod miša i pileta, gen Sox14 je eksprimiran u određenom setu neuralnih progenitorskih ćelija i u određenim tipovima zrelih neurona u mozgu. Za članove SOXB1 podgrupe, kojoj pripadaju geni SOX1, SOX2 i SOX3, poznato je da doprinose održavanju neuralnih progenitorskih ćelija u stanju proliferacije, sprečavajući proces neuralne diferencijacije, dok se za članove SOXB2 podgrupe, koja obuhvata gene SOX14 i SOX21, pretpostavlja da imaju ulogu u određivanju sudbine neuralnih progenitora, indukujući proces neurogeneze. Smatra se da nivo ekspresije SOX14 i SOX21 proteina tokom neuralnog razvića određuje da li će ćelija ostati neuralni progenitor ili će započeti proces neurogeneze. Iako je okarakterisan kao marker neurona, u ovoj tezi je pokazano da se gen SOX14 eksprimira u pluripotentnim ćelijama, neuronima, kao i u drugim tipovima ćelija koje se dobijaju nakon neuralne diferencijacije pluripotentnih NT2/D1 i P19 ćelija in vitro. Takođe, u ovoj tezi je pokazano da je profil ekspresije SOX14 proteina tokom neuralne diferencijacije sličan profilu ekspresije markera postmitotičkih neurona β-III-Tubulina kod NT2/D1 i P19 ćelija. Rezultati prikazani u ovoj doktorskoj disertaciji su pokazali da izlaskom ćelija iz stanja pluripotentnosti i ulaskom u proces neuralne diferencijacije dolazi do povećanja nivoa ekspresije SOX14 proteina. Pokazano je da SOX14 protein ima preklapajući profil ekspresije sa SOXB1 proteinima tokom procesa neuralne diferencijacije in vitro, a dobijeni rezultati su ukazali na specifičnu regulaciju ekspresije gena SOX14 na post-transkripcionom nivou u pluripotentnim ćelijama. Novija istraživanja ukazuju da je ekspresija gena SOX14 izmenjena u maligno transformisanim ćelijama. Naime, istraživanja su pokazala da je gen SOX14 metilovan u tkivima poreklom od tumora, i to naročito u tkivima poreklom od karcinoma grlića materice. Dok studije metilacionog statusa na uzorcima tkiva pacijentkinja obolelih od ovog karcinoma ukazuju na odsustvo ekspresije gena SOX14 i njegovu potencijalnu ulogu tumor supresora, in vitro studije na ćelijama poreklom od karcinoma grlića materice ukazuju na onkogeni potencijal ovog gena. Upravo je kontradiktornost ovih podataka otvorila nove pravce istraživanja usmerene na detaljnu analizu ekspresije i uloge gena SOX14 u regulaciji malignog fenotipa ćelija poreklom od karcinoma grlića materice. Rezultati prikazani u ovoj doktorskoj disertaciji su pokazali da SOX14 ostvaruje funkciju tumor supresora u HeLa ćelijama aktivacijom p53 signalnog puta koji se smatra jednim od najvažnijih signalnih puteva u ćeliji..." ]
[ "SOX14 might have an essential role during development due to the high evolutionary conservation and lack of any known mutated phenotype associated with this gene. Although the first SOX14 gene in vertebrates was cloned and characterized two decades ago, the function of this gene is largely unknown. This doctoral dissertation provides an insight into SOX14 expression and its roles during neural differentiation in vitro and in regulation of malignant phenotype of cervical carcinoma cells. During the neural development, the expression of Sox14 gene is restricted to a defined set of neural progenitors and the precise subset of neurons in the brain. Because of its specific expression in the brain, SOX14 is recognised as a marker of neurons. While SOXB1 subgroup of genes, including SOX1, SOX2 and SOX3 genes, maintains neural progenitors' identity, it is proposed that SOXB2 subgroup, comprising SOX14 and SOX21 genes, are important for induction of neurogenesis. It is suggested that the fine balance between expressions of these proteins during early stages of neural development determines neural progenitor identity. Results presented in this thesis show that SOX14 is expressed in pluripotent cells, as well as in neurons and non-neuronal differentiated cells. Also, it is shown that the expression profile of SOX14 correlates to the expression profile of neuronal marker β-III-Tubulin and that the exit of cells from the pluripotent state toward neural differentiation is accompanied with the increased expression of SOX14. It is also shown that the expression of SOX14 overlaps with the expression of SOXB1 proteins during neural differentiation in vitro. The presented results also imply the specific post-transcriptional mechanism of regulation of SOX14 expression in pluripotent cells. Recently it became evident that SOX14 has a role during malignant transformation of the cells. Previous studies showed that SOX14 is methylated in tissues originating from several tumors, including cervical carcinoma. Results obtained by the analysis of the methylation status of SOX14 gene in tissues from cervical carcinoma suggested its potential role as a tumor suppressor, while, on the other hand, in vitro studies suggested that SOX14 exerts oncogenic potential in cervical carcinoma cells. These contradictory data opened up the possibility for further study of the role of SOX14 gene in cervical carcinoma. Results presented in this thesis show that SOX14 exerts tumor suppressor activity in HeLa cells through activation of p53 signaling pathway, one of the most important signaling pathways in cell. In particular, increased expression of SOX14 leads to stabilization of p53 protein by increasing the level of phosphorylated form of p53 protein in HeLa cells. Phosphorylated p53 acts as a transcriptional regulator of target genes that have an important role in tumor growth prevention. The increased expression level of SOX14 and stabilization of p53 are followed by induction of the expression of p53 target genes, mainly involved in apoptosis and cell cycle regulation, such as pro-apoptotic BAX gene and cyclin-dependent kinase inhibitor CDKN1A/p21Waf1/Cip1. Increased expression of SOX14, through activation of a p53 signaling pathway, leads to decreased proliferation, cell cycle arrest and induction of apoptosis..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "SOX14", "neuralna diferencijacija", "SOXB", "karcinom grlića materice", "tumor supresor", "p53", "fosfo-p53", "apoptoza", "p53 signalni put", "CDKN1A/p21Waf1/Cip1" ]
[ "SOX14", "neural differentiation", "SOXB", "cervical carcinoma", "tumor suppressor", "p53", "phosho-p53", "apoptosis", "p53 signaling pathway", "CDKN1A/p21Waf1/Cip1" ]
[ "Analiza ekspresije i uloge humanog gena SOX14 u neuralnoj diferencijaciji in vitro i regulaciji malignog fenotipa" ]
[ "Analysis of the expression and role of the human gene SOX14 in neural differentiation in vitro and in the regulation of malignant phenotype" ]
doctoralThesis
[ "BY-NC-ND" ]
Поповић, Јелена; Стевановић, Милена; Стевановић, Милена; Савић Павићевић, Душанка; Поповић, Јелена; Станисављевић, Данијела Д.;
[ "https://nardus.mpn.gov.rs/bitstream/id/1489/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1490/IzvestajKomisije17837.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1489/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1490/IzvestajKomisije17837.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10049" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/12267
123456789-12267.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:21685/bdef:Content/download
no
Molekularna karakterizacija humanih izolata laktobacila rezistentnih na antibiotike
Molecular characterization of human vaginal lactobacilli resistant to antibiotics
[ "Topisirović, Ljubiša" ]
[ "Fira, Đorđe" ]
Begović, Jelena M.
2020-03-13T23:36:06
[ "2020-03-13T23:36:06", "2020-07-03T08:07:49" ]
2008
[ "https://nardus.mpn.gov.rs/handle/123456789/12267", "http://eteze.bg.ac.rs/application/showtheses?thesesId=7383", "https://fedorabg.bg.ac.rs/fedora/get/o:21685/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=34644751" ]
[ "" ]
[ "" ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "" ]
[ "" ]
[ "Molekularna karakterizacija humanih izolata laktobacila rezistentnih na antibiotike" ]
[ "Molecular characterization of human vaginal lactobacilli resistant to antibiotics" ]
doctoralThesis
[ "BY-NC-ND" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1698/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1698/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_12267" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/11749
123456789-11749.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:20797/bdef:Content/download
no
Modelovanje proboja u gasovima Monte Karlo tehnikom
Modeling of a gas breakdown by Monte Carlo technique
[ "Petrović, Zoran Lj." ]
[ "Cvetić, Jovan", "Đorđević, Antonije" ]
Puač, Marija
2020-01-27T12:20:41
[ "2020-01-27T12:20:41", "2020-07-03T08:35:50" ]
2019-07-10
[ "https://nardus.mpn.gov.rs/handle/123456789/11749", "http://eteze.bg.ac.rs/application/showtheses?thesesId=7126", "https://fedorabg.bg.ac.rs/fedora/get/o:20797/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51823887" ]
[ "Zbog velike zastupljenosti radiofrekvencijskih (RF) plazmi u industriji javila se potreba za definisanjem osnovnih fizičkih principa koji određuju proboj u RF električnim poljima. Uslovi, u vidu probojnog napona i pritiska, mogu se prikazati naponskom probojnom krivom, predstavljenom u V-p ravni. Oblik i položaj krive definisani su kako prirodom pozadinskog gasa tako i karakteristikama spoljašnjeg RF električnog izvora. Ova disertacija ima za cilj da nedvosmisleno definiše fizičke procese koji su odgovorni za proboj pri RF električnim poljima. Za to je korišćena Monte Karlo tehnika. Odabirom slučajnih brojeva, uz poznavanje preseka za određene procese, moguće je verno (čak se može tvrditi egzaktno) opisati kretanje roja naelektrisanih čestica između dve elektrode koje su pobuđene RF generatorom. Izgradnja modela je započeta posmatranjem kretanja elektrona u argonu pri frekvenciji od 13,56 MHz. Na primeru argona objašnjeni su osnovni mehanizmi proboja koji definišu oblik naponske probojne krive. Nadogradnja modela, kao verodostojne predstave RF proboja, uključuje efekte na površinama elektroda (refleksiju i emisiju sekundarnih elektrona), uticaj promene frekvencije i dvofrekvencijskih izvora, kao i teških čestica. Model je primenjen na različite gasove: helijum, kiseonik, sintetički vazduh i atmosfera Marsa." ]
[ "The wide range of radiofrequency (RF) plasma applications in industry rely on research of fundamental physical processes responsible for the RF breakdown. Conditions, such as the breakdown voltage and pressure, can be presented by breakdown voltage curves in the V-p plane. The shape and coordinates of the curve are defined by the nature of the background gas and characteristics of the applied RF electric field. The goal of this dissertation is to define physical processes responsible for the RF breakdown. For that purpose a Monte Carlo computational code has been used. If one knows cross sections for different processes by employing a random number generator, the transport of electrons between two electrodes can be determined. The development of the RF breakdown model begun by observing the motion of electrons in argon at the field frequency of 13.56 MHz. Argon was used to explain the basic mechanisms that define the shape of the breakdown voltage curve. Further upgrade of the model involved inclusion of the surface effects on electrodes (reflection and secondary electron emission), the influence of frequency and dual frequency RF power supplies, as well as the influence of heavy particles on the breakdown. The model was applied to different gases: helium, oxygen, and synthetic air, as well as the atmosphere of Mars." ]
application/pdf
sr
Универзитет у Београду, Електротехнички факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc/4.0/" ]
Универзитет у Београду
[ "radiofrekvencijski proboj", "radiofrekvencijske plazme", "kretanje naelektrisanih čestica", "Monte Karlo tehnika" ]
[ "Radiofrequency breakdown", "Radiofrequency plasma", "Charged particles transport", "Monte Carlo technique" ]
[ "Modelovanje proboja u gasovima Monte Karlo tehnikom" ]
[ "Modeling of a gas breakdown by Monte Carlo technique" ]
doctoralThesis
[ "BY-NC" ]
[ "http://nardus.mpn.gov.rs/bitstream/id/5732/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/5732/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/5733/IzvestajKomisije21626.pdf", "http://nardus.mpn.gov.rs/bitstream/id/5733/IzvestajKomisije21626.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_11749" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/10971
123456789-10971.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:19549/bdef:Content/download
no
Epilitske zajednice silikatnih algi - sezonska dinamika i procena ekološkog statusa odabranih reka istočne i jugoistočne Srbije
Epilithic diatom communities - seasonal dynamics and ecological status assessment of selected rivers from the eastern and southeastern Serbia
[ "Krizmanić, Jelena" ]
[ "Subakov Simić, Gordana", "Simić, Snežana" ]
Jakovljević, Olga
2019-04-22T11:59:02
[ "2019-04-22T11:59:02", "2020-07-03T08:07:36" ]
2019-02-13
[ "https://nardus.mpn.gov.rs/handle/123456789/10971", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6718", "https://fedorabg.bg.ac.rs/fedora/get/o:19549/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025213362" ]
[ "Analizom zajednice silikatnih algi moņe se dobiti slika opńteg stanja, ekolońkog integriteta i kvaliteta vode odreĊenog vodenog ekosistema. U cilju implementacije Okvirne direktive o vodama, u Srbiji je doneta zakonska regulativa na osnovu koje je obavezna procena ekolońkog statusa povrńinskih voda na osnovu bentosne zajednice silikatnih algi. Predmet ove doktorske disertacije je floristiĉka studija epilitskih zajednica silikatnih algi, njihova sezonska dinamika i primena dijatomnih indeksa kao relevantnih parametara u proceni ekolońkog statusa odabranih reka istoĉne i jugoistoĉne Srbije. Algolońki i fiziĉko-hemijski uzorci su sakupljeni ńest puta u periodu od dvanaest meseci sa ńest lokaliteta duņ toka reke Vrle i reke Crnice, odnosno sa pet lokaliteta duņ toka reke Mlave i Radovanske reke. Pri izboru lokaliteta vodilo se raĉuna o poloņaju pastrmskog ribnjaka i njegovom uticaju kao potencijalnom izvoru zagaĊenja ovih reka. Tokom istraņivanog perioda identifikovano je ukupno 375 taksona epilitskih silikatnih algi u Vrli, Mlavi, Crnici i Radovanskoj reci. Pored ugroņenih taksona, identifikovani su i taksoni koji se smatraju retkim. Od ukupnog broja identifikovanih, 50 taksona je prvi put zabeleņeno u flori algi Srbije. Rod Decussata predstavlja novi rod za floru algi Srbije. CCA analiza uticaja ekolońkih parametara na distribuciju taksona epilitske zajednice silikatnih algi iz 4 istraņivane reke je pokazala da ribnjaci utiĉu na fiziĉko-hemijske osobine vode, a samim tim na sastav epilitske zajednice. Kao znaĉajni fiziĉko-hemijski faktori izdvojili su se NH4+, NH3 i EP. Na osnovu većine dijatomnih indeksa voda sve ĉetiri istraņivane reke je dobrog ekolońkog statusa (II klasa kvaliteta). Prema zakonskoj regulativi Srbije, na osnovu biolońkih (fitobentos) i fiziĉko-hemijskih elemenata, ekolońki status vode istraņivanog dela reke Vrle je dobar (II klasa), a reke Mlave umeren (III klasa). Konaĉan ekolońki status Crnice i Radovanske reke je procenjen kao slab (IV klasa)..." ]
[ "By analyzing the benthic diatom community, a picture of the general condition, ecological integrity and water quality of a particular aquatic ecosystem can be obtained. In order to implement the Water Framework Directive, a legislation has been adopted in the Republic of Serbia that requires an assessment of the ecological status of surface waters based on the benthic diatoms. The aim of this PhD thesis was the floristic analysis of the epilithic diatom communities, their seasonal dynamics and the application of diatom indices as relevant parameters in the ecological status assessment of the selected rivers from the Eastern and Southeastern Serbia. Algological and physico-chemical samples were collected six times over a period of twelve months from six sites along the Vrla and Crnica rivers, and from five sites along the Mlava and Radovanska rivers. In selecting the sites taken into account the position of a trout ponds and its impact as a potential sources of pollution of the rivers. A total of 375 epilithic diatom taxa in Vrla, Mlavi, Crnica and Radovanova rivers were identified during the investigated period. Some endangered and rare taxa were identified. Of the total number of identified taxa, 50 taxa were new records for the Serbian algal flora. The genus Decussata, with the rare species D. hexagona, was recorded for the first time in Serbia. The CCA analysis of the impact of ecological parameters on the distribution of the diatom taxa from the investigated rivers has shown that the fish ponds affect the water physico-chemical properties and, consequently, the composition of the epilithic diatom communities. NH4+, NH3 and EP have been identified as important physical-chemical factors..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/37009/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "epilitske silikatne alge", "sezonska dinamika", "floristiĉki sastav", "dijatomni indeksi", "ekolońki status", "Vrla", "Mlava", "Crnica", "Radovanska reka" ]
[ "epilithic diatoms", "seasonal dynamics", "floristic composition", "diatom indices", "ecological status", "Vrla River", "Mlava River", "Crnica River", "Radovanska River" ]
[ "Epilitske zajednice silikatnih algi - sezonska dinamika i procena ekološkog statusa odabranih reka istočne i jugoistočne Srbije" ]
[ "Epilithic diatom communities - seasonal dynamics and ecological status assessment of selected rivers from the eastern and southeastern Serbia" ]
doctoralThesis
[ "BY-NC-ND" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1652/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1653/IzvestajKomisije19683.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1653/IzvestajKomisije19683.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1652/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_10971" ]
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https://nardus.mpn.gov.rs/handle/123456789/2037
123456789-2037.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:3461/bdef:Content/download
no
Ekološka analiza zajednice makrozoobentosa reke Nišave
Ecological analysis of macrozoobenthos communities in the Nišava River
[ "Krpo-Ćetković, Jasmina" ]
[ "Krpo-Ćetković, Jasmina", "Živić, Ivana", "Živić, Ivana", "Marković, Zoran" ]
Savić, Ana V.
2016-01-05T11:45:31
[ "2016-01-05T11:45:31", "2020-07-03T08:08:24" ]
2012-10-06
[ "https://nardus.mpn.gov.rs/handle/123456789/2037", "http://eteze.bg.ac.rs/application/showtheses?thesesId=71", "https://fedorabg.bg.ac.rs/fedora/get/o:3461/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024510386" ]
[ "Doktorska disertacija obuhvata ekološku analizu zajednica makrozoobentosa reke Nišave i njihovu zavisnost od biotičkih i abiotičkih faktora. Istraživanja su obavljena mesečnom dinamikom na dvanaest lokaliteta od maja 2006. do aprila 2007. godine. U disertaciji se diskutuje zavisnost koncentracije hlorofila a i mase perifitona od abiotičkih faktora. Sa druge strane, razmatran je pritisak (na osnovu brojnosti) filtratornih vrsta zajednice makroinvertebrata na količinu fitoplanktona (izraženu preko hlorofila a) i pritisak grebača na masu perifitona. Dobijen je predikcioni model za izračunavanje koncentracije hlorofila a korišćenjem multiple linearne regresije. Model ima značajnu predikcionu moć (sig=0,003). Na osnovu Pirsonovog koeficijenta korelacije konstatovano je da postoji značajna povezanost koncentracije hlorofila a sa mutnoćom (p=0,000), sa ukupnim fosforom (p=0,029) i sa kombinovanom (zbirnom) koncentracijom ukupnog azota i fosfora (p=0,036). Nije utvrđena značajna povezanost sa brojnošću filtratornih organizama, što govori da njihov pritisak nije dovoljno jak da bi limitirao količinu fitoplanktona. Taksonomskom analizom makroinvertebrata reke Nišave utvrđeno je prisustvo 132 vrste iz 4 različita filuma. Filum Platyhelminthes je predstavljen sa 3 vrste iz 2 familije. Filum Annelida obuhvata 23 vrste iz 6 familija. Najveći broj vrsta ovog filuma pripada potklasi Oligochaeta (16 vrsta). Filum Mollusca obuhvata 13 vrsta iz 5 familija, od kojih 11 pripada klasi Gastropoda, a 2 klasi Bivalvia. Najveći broj vrsta faune makroinvertebrata pripada filumu Arthropoda (92 vrste iz 33 familije), od kojih 3 pripadaju subfilumu Crustacea, a sve ostale podfilumu Hexapoda, odnosno klasi Insecta. Rezultati taksonomske analize pojedinačnih grupa u okviru zajednice makroinvertebrata pokazuju da najveći broj vrsta pripada grupi Ephemeroptera (28 vrsta), zatim slede Diptera (26 vrsta) i Trichoptera (20 vrsta). Ostale grupe su zastupljene sa manjim brojem vrsta. Diskutovana je sezonska i longitudinalna dinamika grupa makroinvertebrata i njihova uslovljenost faktorima sredine. Razmatrani su indeksi diverziteta i adekvatnost njihovog korišćenja kao pokazatelja zagađenosti ekosistema, posebno za svaku grupu..." ]
[ "This doctoral dissertation comprises the ecological analysis of macrozoobenthos communities in the Nišava River and their dependence on biotic and abiotic factors. The studies followed the monthly dynamics at 12 sites, from May 2006 to April 2007. The dissertation is discussing the dependence of chlorophyll a concentration and periphyton mass on abiotic factors, as well as the pressure (based on abundance) of filtrating species in the macroinvertebrate community on the amount of phytoplankton (expressed as chlorophyll a) and pressure of scraper species on periphyton mass. The prediction model for calculating chlorophyll a concentration was derived from the multiple linear regression. This model has a significant prediction power (sig=0.003). The Pearson's correlation coefficient was used to determine the correlation of chlorophyll a concentration with water turbidity (p=0.000), total phosphorus (p=0.029) and combined (summative) concentration of total nitrogen and total phosphorus (p=0.036). No significant connection with the abundance of filtrating organisms was determined, which indicates that their pressure was not strong enough to limit the quantity of phytoplankton. The taxonomic analysis of macroinvertebrates in the Nišava River yielded the presence of 132 species from 4 different phyla. The phylum Platyhelminthes was represented with 3 species from 2 families. The phylum Annelida was represented with 23 species from 6 families. Most recorded species from this phylum belong to the subclass Oligochaeta (16 species). The phylum Mollusca was represented with 13 species from 5 families, of which 11 were from the class Gastropoda and 2 from the class Bivalvia. Most species of the macroinvertebrate fauna belong to the phylum Arthropoda (92 species from 33 families), of which 3 were from the subphylum Crustacea, while all others belong to the subphylum Hexapoda and class Insecta. The results of the taxonomic analysis of individual groups within the macroinvertebrate community have shown that most species belong to the group Ephemeroptera (28 species), followed by Diptera (26 species), and Trichoptera (20 species). Other groups were represented with a smaller number of species. The study also includes the discussion on seasonal and longitudinal dynamics of macroinvertebrate groups and the effect of environmental factors on their presence. The diversity indices and their suitability as indicators of ecosystem pollution were discussed for each group separately..." ]
application/pdf
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Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "faktori sredine", "faunistička raznovrsnost", "trofičke gilde", "makroinvertebrate", "reka Nišava", "struktura zajednica", "zagađenost vode" ]
[ "environmental factors", "faunistic diversity", "trophic guilds", "macroinvertebrates", "Nišava River", "community structure", "water pollution" ]
[ "Ekološka analiza zajednice makrozoobentosa reke Nišave", "Ecological analysis of macrozoobenthos communities in the Nišava River" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Крпо-Ћетковић, Јасмина; Марковић, Зоран; Живић, Ивана; Крпо-Ћетковић, Јасмина; Живић, Ивана; Савић, Aна В.; Еколошка анализа заједнице макрозообентоса реке Нишаве; Еколошка анализа заједнице макрозообентоса реке Нишаве;
[ "https://nardus.mpn.gov.rs/bitstream/id/1882/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1882/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2037" ]
10.2298/bg20121006savic
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/8249
123456789-8249.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:15646/bdef:Content/download
no
Značaj razlika nivoa imunoglobulina specifičnih za melanin i tirozinazu u antitumorskoj imunosti bolesnika sa melanomom
The importance of the differences in immunoglobulin levels specific for melanin and tyrosinase in antitumor immunity in patients with melanoma
[ "Božić, Biljana" ]
[ "Matić, Ivana", "Brajušković, Goran" ]
Đorđić-Crnogorac, Marija J.
2017-06-02T18:42:10
[ "2017-06-02T18:42:10", "2020-07-03T08:08:08" ]
2017-02-13
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=4994", "https://nardus.mpn.gov.rs/handle/123456789/8249", "https://fedorabg.bg.ac.rs/fedora/get/o:15646/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025151922" ]
[ "Melanom je visoko imunogeno maligno oboljenje. Melanomski antigeni imaju sposobnost imunizacije i stimulacije sinteze specifičnih antitela. Antitela specifična za iste, melanomske antigene, koji su prisutni kako na neoplastično transformisanim, tako i na neizmenjenim melanocitima, pronađeni su i kod bolesnika sa vitiligom. Pojava hipopigmentacija sličnih vitiligu kod bolesnika sa melanomom utiče na bolju prognozu preživljavanja, što ukazuje na značaj veze između tumorske imunosti i autoimunosti kod ovih bolesnika. Ćelije melanoma karakteriše povećano prisustvo melanina i tirozinaze, a u serumu bolesnika sa metastatskim melanomom uočena je tirozinazna aktivnost. Dipeptidil peptidaza IV (DPPIV/CD26) je transmembranski glikoprotein koji se eksprimira na površini limfocita, i predstavlja važan marker aktivacije ćelija imunskog sistema. Upravo su limfociti jedan od najznačajnijih izvora solubilne forme ovog proteina u serumu. DPPIV ima značajnu ulogu u regulaciji imunskih funkcija i procesu neoplastične transformacije. Osnovni cilj ovog istraživanja je bio da se ispitaju razlike u nivoima IgG, IgA i IgM klasa antitela specifičnih za melanin i tirozinazu u bolesnika sa melanomom u odnosu na bolesnike sa vitiligom i zdrave osobe. Od značaja je bilo i da se ispita postojanje razlika u humoralnom imunskom odgovoru između bolesnika sa melanomom bez metastaza i bolesnika sa melanomom i metastazama. S ciljem dodatne karakterizacije imunskog odgovora, određivan je procenat CD16+ i CD16+CD56+ limfocita, i CD89+ granulocita kod bolesnika sa melanomom, kao i bolesnika sa vitiligom i zdravih osoba, a zatim je ispitivana povezanost nivoa antitela i CD16/CD89+ ćelija u grupi bolesnika sa melanomom..." ]
[ "Melanoma is a highly immunogenic malignancy. Melanoma antigens are capable of immunization and stimulation of the synthesis of specific antibodies. Antibodies specific for same, melanoma antigens, which are present both on the neoplastically transformed, as well as on the non-transformed melanocytes, were also found in patients with vitiligo. The presence of vitiligo-like hypopigmentations in patients with melanoma is associated with a better survival prognosis, indicating the importance of the link between tumor immunity and autoimmunity. The increased presence of melanin and tyrosinase is characteristic of melanoma cells, while the tyrosinase activity was observed in the sera of patients with metastatic melanoma. Dipeptidyl peptidase IV (DPPIV/CD26) is a transmembrane glycoprotein which is expressed on the surface of lymphocytes, and represents an important marker of activation of immune system cells. Lymphocytes represent one of the major sources of soluble form of this protein in the serum. DPPIV is implicated in the regulation of immune functions and neoplastic transformation. The main goal of this study was to examine the differences in the levels of IgG, IgA and IgM classes of antibodies specific for melanin and tyrosinase in patients with melanoma, compared with the group of patients with vitiligo and the group of healthy individuals. The examination of the presence of differences in the humoral immune response between patients with melanoma without metastases and patients with melanoma and metastases was also significant for our research. To further characterize the immune response, it was important to determine the percentage of CD16+ and CD16+CD56+ lymphocytes and CD89+ granulocytes in patients with melanoma, as well as patients with vitiligo and healthy persons, and also to investigate the connection between the levels of antibodies and CD16/CD89+ cells in the group of patients with melanoma..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175011/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Melanom", "melanin", "tirozinaza", "vitiligo", "antitela", "CD16+ limfociti", "CD89+ granulociti", "DPPIV/CD26" ]
[ "Melanoma", "melanin", "tyrosinase", "vitiligo", "antibodies", "CD16+ lymphocytes", "CD89+ granulocytes", "DPPIV/CD26" ]
[ "Značaj razlika nivoa imunoglobulina specifičnih za melanin i tirozinazu u antitumorskoj imunosti bolesnika sa melanomom" ]
[ "The importance of the differences in immunoglobulin levels specific for melanin and tyrosinase in antitumor immunity in patients with melanoma" ]
doctoralThesis
[ "BY-NC-ND" ]
Божић, Биљана; Матић, Ивана; Брајушковић, Горан; Ђорђић-Црногорац, Марија Ј.; Значај разлика нивоа имуноглобулина специфичних за меланин и тирозиназу у антитуморској имуности болесника са меланомом; Значај разлика нивоа имуноглобулина специфичних за меланин и тирозиназу у антитуморској имуности болесника са меланомом;
[ "https://nardus.mpn.gov.rs/bitstream/id/1813/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1814/IzvestajKomisije9473.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1813/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1814/IzvestajKomisije9473.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_8249" ]
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https://nardus.mpn.gov.rs/handle/123456789/2041
123456789-2041.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:3033/bdef:Content/download
no
Ispitivanje senzitivnosti genoma kod pacijenata sa bolestima hromozomske nestabilnosti .
Genome sensitivity in patients with chromosomal instability disorders
[ "Guć-Šćekić, Marija" ]
[ "Milašin, Jelena", "Vuković-Gačić, Branka" ]
Ćirković, Sanja S.
2016-01-05T11:45:33
[ "2016-01-05T11:45:33", "2020-07-03T08:08:39" ]
2012-10-29
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=48", "https://nardus.mpn.gov.rs/handle/123456789/2041", "https://fedorabg.bg.ac.rs/fedora/get/o:3033/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024511666" ]
[ "U cilju ispitivanja senzitivnosti genoma i potvrde klinički postavljene dijagnoze bolesti hromozomske nestabilnosti, korišćeni su uzorci dve grupe pacijenata sa kliničkom slikom: Fankonijeve anemije (FA) i Nijmegenovog sindroma nestabilnosti (NSN) (eng. Nijmegen Breakage syndrome, NBS). Specifična hipersenzitivnost ćelija obolelih od FA na diepoksibutan (DEB), odnosno kod obolelih od NSN na bleomicin (BLC), iskorišćena je u diferencijalno dijagnostičke svrhe. Studija je obuhvatila ukupno 100 pacijenata: 90/100 sa kliničkom slikom FA i 10/100 pacijenata sa kliničkim znacima NSN, koji su dijagnostikovani na Institutu za zdravstvenu zaštitu majke i deteta Srbije „Dr Vukan Čupić“. U detekciji DEB-om indukovane hromozomske nestabilnosti obolelih od FA primenjene su standardne citogenetičke metode. Za potvrdu ili isključenje mozaičnog fenotipa FA korišćene su dodatne metode: analiza oštećenja DNK komet testom i protočno citometrijska analiza zastoja ćelija u G2 fazi ćelijskog ciklusa. Hromozomska osetljivost ćelija obolelih od NSN je analizirana primenom bleomicinovog testa (BLC test). Molekularna analiza prisustva ili odsustva c.657_661del5 mutacije u NBN genu obolelih od NSN, urađena je korišćenjem modifikovane PCR metode uz analizu heterodupleksa. Kod 11,11% (10/90) pacijenata pod sumnjom da boluju od FA (FA grupa), otkrivena je povećana hromozomska osetljivost na DEB u odnosu na kontrolnu grupu (Mann-Whitneyev test: p < 0,05), dok su kod 3,33% (3/90) pacijenata dobijene granične vrednosti iste analize (FA* grupa). U cilju preciznijeg klasifikovanja pacijenata u FA grupi, primenjen je indeks hromozomske fragilnosti. Na ovaj način je u FA grupi, kod 40% (4/10) pacijenata otkriven ćelijski fenotip FA nemozaičnog tipa, dok je kod 60% (6/10) pacijenata dobijen mozaični odgovor na DEB. Kod svih pacijenata sa mozaičnim fenotipom FA, kao i kod dva pacijenta sa graničnim vrednostima diepoksibutanovog testa (DEB testa), daljim analizama primenom komet testa i analizom zastoja ćelija u G2 fazi, potvrđena je dijagnoza FA. U drugoj grupi od 10 bolesnika sa sumnjom da boluju od NSN analizirana je BLC-om indukovana hromozomska nestabilnost, koja je bila povećana kod 40% (4/10) pacijenata (NBS grupa). Kod 30% (3/10) pacijenata dobijeni rezultati su se preklapali sa rezultatima kontrolne grupe (ne-NBS grupa), dok je kod preostala tri pacijenta analiza bila neuspešna zbog slabog mitotskog indeksa. Molekularnim analizama je otkriveno homozigotno prisustvo c.657_661del5 mutacije u NBN genu kod sva četiri pacijenta iz NBS grupe, kao i kod tri pacijenta kod kojih je citogenetička analiza bila neuspešna, i tako potvrđena konačna dijagnoza NSN. Na osnovu dobijenih rezultata može se zaključiti da su analize korišćene u ovoj studiji omogućile potvrdu klinički postavljene dijagnoze FA i NSN, što opravdava njihovu dalju primenu u diferencijalnoj dijagnostici ovih oboljenja." ]
[ "In order to determine the sensitivity of the genome and to confirm a clinical diagnosis of chromosomal instability disorders, blood samples from patients with clinical suspicion of Fanconi’s anemia (FA) and Nijmegen Breakage syndrome (NBS), were collected for chromosome fragility evaluation by the diepoxybutane (DEB) and bleomycin (BLC) tests. The study considered a total of 100 patients: 90/100 with the hematological and/or congenital phenotypic symptoms reminiscent of FA and 10/100 patients with clinical features of NBS, all diagnosed at the Mother and Child Health Care Institute of Serbia \"Dr Vukan Čupić”. The DEB-induced chromosomal fragility analysis in patients with FA symptoms was carried out by using standard cytogenetic methods. The additional methods, such as: DNA damage analysis by comet assay and flow cytometric analysis of G2 phase cell cycle arrest, were used in order to confirm FA phenotype in patients with mosaic cytogenetic response to DEB. Chromosomal sensitivity of cells in patients with NBS symptoms was analyzed using a BLC test. Molecular analysis for the presence of the c.657_661del5 mutation in NBN gene in patients with NBS was carried out using modified PCR method and heteroduplex analysis on PAGE gel. In this study 11.11% (10/90) of patients with clinical suspicion of FA were found to have an increased chromosomal sensitivity to DEB (FA group), comparing to healthy controls (Mann-Whitney test: p < 0.05), while 3.33% (3/90) of patients showed borderline results of the same analysis (FA* group). The chromosome fragility index was used in order to provide a clear cut-off diagnostic level distinguishing FA mosaic from other patients in the FA group. In this group, 40% (4/10) of FA patients revealed non-mosaic FA phenotype, while the remaining 60% (6/10) of FA patients showed a mosaic response to DEB. In all of FA mosaic patients, and two patients with borderline DEB-sensitivity, further analyses were performed using comet assay and analysis of G2 phase cell cycle arrest, in order to confirm the diagnosis of FA. In the second group of 10 patients with clinical suspicion of NBS, the cytogenetic analysis revealed an increased chromosomal sensitivity to BLC in 40% (4/10) of them (NBS group). The BLC-induced chromosome fragility values in 30% (3/10) of these patients were overlapping those in the control group (non-NBS group). In the remaining of three patients the cytogenetic analysis was unsuccessful, due to the low mitotic index. Molecular analysis confirmed the presence of homozygous c.657_661del5 mutation in all seven NBS patients, including three patients with unsuccessful cytogenetic analysis, which confirmed the final diagnosis of NBS. Based on these results it can be concluded that the analyses used in this study are useful in confirmation of clinical diagnosis of FA and NBS, which justifies their further application in the differential diagnosis of these two diseases." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Fankonijeva anemija (FA)", "Nijmegenov sindrom nestabilnosti (NSN)", "diepoksibutan (DEB)", "bleomicin (BLC)", "hromozomska nestabilnost", "citogenetika", "komet test", "analiza ćelijskog ciklusa", "c.657_661del5 mutacija" ]
[ "Fanconi’s anemia (FA)", "Nijmegen Breakage syndrome (NBS)", "diepoxybutane (DEB)", "bleomycin (BLC)", "chromosomal fragility", "cytogenetic", "comet assay", "cell cycle", "c.657_661del5 mutation" ]
[ "Ispitivanje senzitivnosti genoma kod pacijenata sa bolestima hromozomske nestabilnosti .", "Genome sensitivity in patients with chromosomal instability disorders" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Гућ-Шћекић, Марија; Вуковић-Гачић, Бранка; Милашин, Јелена; Ћирковић, Сања С.; Испитивање сензитивности генома код пацијената са болестима хромозомске нестабилности .; Испитивање сензитивности генома код пацијената са болестима хромозомске нестабилности .;
[ "https://nardus.mpn.gov.rs/bitstream/id/1929/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1929/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2041" ]
10.2298/bg20121029cirkovic
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https://nardus.mpn.gov.rs/handle/123456789/11823
123456789-11823.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:20861/bdef:Content/download
no
Antimikrobni i antitumorski potencijal sekreta i morfološka karakterizacija žlezdanih struktura pigidijalnih žlezda odabranih vrsta trčuljaka (Coleoptera: Carabidae)
Antimicrobial and antitumor potential of the secretions and morphological characterization of the glandular structures of the pygidial glands of selected species of ground beetles (Coleoptera: Carabidae)
[ "Ćurčić, Srećko B." ]
[ "Ćirić, Ana", "Soković, Marina", "Pantelić, Dejan", "Dimkić, Ivica" ]
Nenadić, Marija Z.
2020-02-07T10:29:21
[ "2020-02-07T10:29:21", "2020-07-03T08:08:03" ]
2019-12-23
[ "https://nardus.mpn.gov.rs/handle/123456789/11823", "http://eteze.bg.ac.rs/application/showtheses?thesesId=7168", "https://fedorabg.bg.ac.rs/fedora/get/o:20861/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025241266" ]
[ "Ispitivanje pigidijalnog žlezdanog kompleksa nalazi značajno mesto u karabidologiji, prevashodno sa stanovišta morfologije i taksonomije, ali u novije vreme i ostalih aspekata koji se mogu odnositi na potencijalnu upotrebu primarnih produkata ovih žlezda u biomedicinskim istraživanjima. Ovaj kompleks predstavlja sistem egzokrinih žlezda koji se sastoji od sekretornih lobusa izgrađenih od sekretornih ćelija, gde se sekreti sintetišu, zatim radijalnih sabirnih kanala, glavnog sabirnog kanala, rezervoara, gde se sekreti skladište, i eferentnog kanala, čija je uloga transport sekreta iz rezervoara u spoljašnju sredinu. Primarna funkcija sekreta pigidijalnih žlezda jeste odbrana trčuljaka od potencijalnih predatora iz prirode. U hemijski sastav ovih sekreta ulazi čitav niz jedinjenja koja se uglavnom ubrajaju u aldehide, ketone i organske kiseline. U poslednje vreme postoji interesovanje za istraživanje antimikrobnog i antitumorskog potencijala navedenih smeša sekreta, s obzirom da je prethodno dokazan antimikrobni i antitumorski potencijal pojedinačnih jedinjenja koja se nalaze u njihovom sastavu. Ciljevi ove doktorske disertacije su usmereni ka ispitivanju antimikrobnog i antitumorskog potencijala sekreta pigidijalnih žlezda odabranih vrsta trčuljaka, što može imati značaj u bazičnim biomedicinskim istraživanjima, kao i ka pronalaženju pozadine ovakvih bioloških aktivnosti u ekološkoj funkciji kod ovih insekata, koja bi podobnije bila približena detaljnijom opservacijom pigidijalnih žlezdanih struktura sa morfo-anatomskog aspekta, primenom elektronske i nelinearne mikroskopije, što bi doprinelo i boljem razumevanju fiziologije ovog sistema. U prvom delu doktorske disertacije koji obuhvata biomedicinski aspekt, posmatrana je po prvi put antimikrobna aktivnost sekreta pigidijalnih žlezda 6 odabranih vrsta trčuljaka na humane patogene (osam laboratorijskih i kliničkih bakterijskih sojeva i osam patogenih mikromiceta), kao i citotoksičnost sekreta 4 odabrane vrste trčuljaka na netumorske (primarne ćelije jetre izolovane iz organizma svinje) i humane tumorske ćelijske linije (nesitnoćelijski karcinom pluća, vii cervikalni karcinom, adenokarcinom dojke i hepatocelularni karcinom)..." ]
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application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Carabidae", "pigidijalne žlezde", "humani patogeni", "antimikrobna aktivnost", "mikrodiluciona metoda", "citotoksični efekat", "antitumorski potencijal", "sulforodamin B esej", "guano", "SEM", "TPEF", "NLM" ]
[ "Carabidae", "pygidial glands", "human pathogens", "antimicrobial activity", "microdilution method", "cytotoxic effect", "antitumor potential", "sulforhodamine B assay", "guano", "SEM", "TPEF", "NLM" ]
[ "Antimikrobni i antitumorski potencijal sekreta i morfološka karakterizacija žlezdanih struktura pigidijalnih žlezda odabranih vrsta trčuljaka (Coleoptera: Carabidae)" ]
[ "Antimicrobial and antitumor potential of the secretions and morphological characterization of the glandular structures of the pygidial glands of selected species of ground beetles (Coleoptera: Carabidae)" ]
doctoralThesis
[ "BY-NC-ND" ]
[ "https://nardus.mpn.gov.rs/bitstream/id/1778/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1779/IzvestajKomisije21830.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1778/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1779/IzvestajKomisije21830.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_11823" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
[ "" ]
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[ "" ]
[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/2051
123456789-2051.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:2208/bdef:Content/download
no
Molekulski mehanizmi strukturnog remodeliranja mrkog masnog tkiva pacova indukovanog insulinom
Molecular mechanisms of insulin-induced rat brown adipose tissue structural remodelling
[ "Korać, Aleksandra" ]
[ "Stančić, Ana", "Korać, Bato", "Janković, Aleksandra", "Buzadžić, Biljana" ]
Markelić, Milica B.
2016-01-05T11:46:10
[ "2016-01-05T11:46:10", "2020-07-03T08:08:28" ]
2012-05-04
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=17", "https://nardus.mpn.gov.rs/handle/123456789/2051", "https://fedorabg.bg.ac.rs/fedora/get/o:2208/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=" ]
[ "U svetlu sve većeg broja dokaza koji ukazuju na prisustvo metabolički aktivnog mrkog masnog tkiva (BAT – brown adipose tissue, engl.) kod odraslih ljudi, kao i na njegovu potencijalnu ulogu u sprečavanju razvoja gojaznosti, insulinske rezistence i metaboličkog sindroma uopšte, sve je više studija o rasvetljavanju metaboličkog značaja ovog tkiva, kao i njegovoj termogenoj stimulaciji u svrhu terapije navedenih poremećaja. Insulin se, kao važan anabolički hormon, smatra značajnim modulatorom strukturne organizacije i funkcije BAT. Cilj ovog istraživanja bio je rasvetljavanje molekulskih osnova insulinomindukovanog strukturnog remodeliranja BAT putem identifikacije mehanizama koji regulišu insulinom-stimulisanu proliferaciju i diferencijaciju ćelija, kao i identifikacije mehanizama koji učestvuju u oštećenjima ćelija i njihovom umiranju. Pacovi soja Wistar su jednom dnevno tretirani fiziološkom (0.4 IU/kg telesne mase) i suprafiziološkom (4 IU/kg telesne mase) dozom insulina, jedan (akutan tretman) ili tri (hroničan tretman) dana. Kao fiziološka kontrola, dve grupe pacova su u istom trajanju tretirane fiziološkim rastvorom (akutna i hronična kontrola). Tri sata nakon poslednje doze, životinje su žrtvovane, a interskapularni depo BAT je izolovan, izmeren i pripremljen za tehnike svetlosne i elektronske mikroskopije. Analiza tkiva izvršena je korišćenjem metoda histohemijskog i imunohistohemijskog bojenja, metoda konfokalne i transmisione elektronske mikroskopije i elektron-disperzivne analize X-zracima (EDX) hemijskog sastava tkiva kao i metoda stereološke i morfometrijske analize. Rezultati su pokazali postojanje strukturnog i funkcijskog remodeliranja BAT, gde dominiraju procesi hipertrofije i hiperplazije ćelija, praćene povećanjem termogenog kapaciteta mrkih adipocita (raste ekspresija dekuplujućeg proteina 1 (UCP1 – uncoupling protein 1, engl.), udeo mitohondrija, procesi mitohondriogeneze). S druge strane, primetno je da se, naročito pri hroničnim i tretmanima visokom dozom insulina, javljaju citotoksični i inhibitorni efekti na pojedine ćelije ili klastere ćelija: raste učestalost ćelijske smrti, ekstravazacija eritrocita i njihovo uklanjanje od strane mrkih adipocita i makrofaga, (evidentno toksično po adipocite sudeći po rastu: ekspresije enzima antioksidativne odbrane; lipidne peroksidacije i nitrozilacije proteina, učestalosti lipofuscinogeneze). Takođe se pri hronično visokoj hiperinsulinemiji smanjuje ekspresija regulatora termogeneze (PGC-1α - peroxisome proliferator-activated receptor – γ coactivator - 1α, engl.) ukazujući da produženo izlaganje visokim dozama insulina, može da inhibira termogeni odgovor tkiva, o čemu svedoči i povećana učestalost mitohondrijalnih oštećenja. Povećana ekspresija proinflamatornih citokina (TNF-α - tumor-necrosis factor – α, engl., interleukina 6), nukleusnog faktora – kB (NF-kB) i makrofagnog inflamatornog proteina - 3β (MIP-3β) u pojedinačnim zrelim, multilokulusnim adipocitima, preadipocitima i unilokulusnim adipocitima ukazuje na njihovu potencijalnu ulogu u lokalnom smanjenju insulinske senzitivnosti. U vezi sa hiperplazijom ćelija, primećeno je da su adipogeneza i angiogeneza u BAT vremenski i prostorno povezane, tj. da postoji udruženost navedenih procesa, koja ukazuje na potrebu udruženog nastanka adipocita i endotelskih ćelija, u svrhu efikasnog strukturno-funkcijskog remodeliranja tkiva. Sumarno, može se zaključiti da hiperinsulinemija ima značajan efekat na BAT – potvrđeno je anaboličko dejstvo insulina i važnost njegove uloge u stimulaciji BAT, ali je i pokazana toksičnost visoke doze i hroničnog izlaganja na ćelije BAT; što je sve doprinelo rasvetljavanju molekulskih mehanizama u osnovi strukturnog remodeliranja ovog tkiva. Takođe, pokazano je da se visoka plastičnost BAT ogleda u postojanju funkcionalnih klastera ćelija, u kojima se odvijaju svi važni tkivni procesi (adipo/angiogeneza, ćelijska smrt, eritrofagocitoza), što ukazuje na pravilnu uređenost tkivne organizacije i strukturnog remodeliranja, kao preduslova za pravilno funkcionisanje tkiva, kako u fiziološkim, tako i u uslovima izmenjene tkivne homeostaze, što je pokazano na modelu hiperinsulinemije." ]
[ "There is an increasing amount of evidence that indicates presence of brown adipose tissue (BAT) in adult humans, and its potential role in prevention of obesity, insulin resistance and metabolic syndrom, in general. That is why number of studies on elucidation of the metabolic importance of this tissue, along with its thermogenic stimulation in order to treat these disorders, increases. As an important anabolic hormone, insulin is considered as a major modulator of BAT structure and function. The aim of this study was to elucidate the molecular basis of insulin-induced structural remodeling of BAT through the identification of mechanisms that regulate insulin-stimulated proliferation and differentiation and through the identification of mechanisms involved in cell damage and death. Wistar strain rats were treated intraperitoneally with physiological (0.4 IU/kg BW) or supraphysiological (4 IU/kg BW ) dose of insulin for one (acute treatment) or three days (chronic treatment), respectively. Two groups of rats served as physiological controls, and were treated with saline (acute and chronic control). Three hours after the last injection the animals were sacrificed and the interscapular portion of BAT was removed, its weight was measured and it was processed for electron and light microscopic examinations. Methods of histochemical staining, immunohistochemical labeling, confocal and transmission electron microscopy, tissue electron-dispersive X-ray microanalysis (EDX) and stereological and morphometric analyses were performed. The results showed the existence of structural and functional remodeling of BAT, based on adipocyte hypertrophy and hyperplasia which are followed by increasing of thermogenic capacity (expression of uncoupling protein 1 (UCP1), cellular proportion of mitochondria, mitochondrial biogenesis). On the other hand, it is obvious that, especially after chronic and high-dose treatments, insulin cytotoxic and inhibitory effects on individual cells or cell clusters occur: incidence of cell death increases, as well as red blood cell extravasation and their removal by brown adipocytes and macrophages (which is evidently toxic for the adipocyte, since expression of antioxidative defense enzymes, lipid peroxidation, protein nitrosilation and lipofuscin formation were increased). Also, during chronically high hyperinsulinemia, expression of peroxisome proliferator-activated receptor - γ coactivator - 1α (PGC-1α), regulator of thermogenesis decreases, indicating that prolonged exposure to high doses of insulin can inhibit thermogenic response of BAT, as evidenced by the increased frequency of mitochondrial damage in brown adipocytes. Increased expression of proinflammatory cytokines (tumornecrosis factor - α (TNF-α), interleukin 6), nuclear factor – kB (NF-kB) and macrophage inflammatory protein - 3β (MIP-3β) in individual mature, multilocular adipocytes, preadipocytes and unilocular adipocytes, demonstrates their potential role in local decrease of insulin sensitivity. Regarding hyperplasia of cells, it was noticed spaciotemporal association between processes of adipogenesis and angiogenesis, which means that there is a connection between these processes, demonstrating the need for the coupled differentiation of adipocytes and endothelial cells, for the purpose of efficient structural and functional remodeling of the tissue. In summary, it can be concluded that hyperinsulinemia has a significant impact on BAT – we confirmed the anabolic effect of insulin, and the importance of its role in BAT stimulation, but also demonstrated the toxicity of high dose and chronic exposure to insulin on BAT cells, all of which contribute to understanding the molecular mechanisms underlying structural remodeling of the tissue. In addition, it was shown that the high plasticity of BAT is reflected in the existence of functional clusters of cells, which take place in all the important processes in the tissue (adipo/angiogenesis, cell death, erythrophagocytosis), indicating the proper arrangement of tissue organization and structural remodeling as a prerequisite for the proper functioning of the tissue under physiological conditions and during altered tissue homeostasis, as shown on the model of hyperinsulinemia." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173055/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Mrko masno tkivo", "insulin", "hiperinsulinemija", "strukturno remodeliranje tkiva", "adipogeneza", "angiogeneza", "termogeneza", "mrki adipocit" ]
[ "Brown adipose tissue", "insulin", "hyperinsulinaemia", "structural tissue remodeling", "adipogenesis", "angiogenesis", "thermogenesis", "brown adipocyte" ]
[ "Molekulski mehanizmi strukturnog remodeliranja mrkog masnog tkiva pacova indukovanog insulinom", "Molecular mechanisms of insulin-induced rat brown adipose tissue structural remodelling" ]
[ "" ]
doctoralThesis
[ "BY-NC-ND" ]
Кораћ, Aлександра; Јанковић, Aлександра; Станчић, Aна; Кораћ, Бато; Бузаджић, Биљана; Маркелић, Милица Б.; Молекулски механизми структурног ремоделирања мрког масног ткива пацова индукованог инсулином; Молекулски механизми структурног ремоделирања мрког масног ткива пацова индукованог инсулином;
[ "https://nardus.mpn.gov.rs/bitstream/id/1890/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1890/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2051" ]
10.2298/bg20120504markelic
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https://nardus.mpn.gov.rs/handle/123456789/5089
123456789-5089.pdf
no
no
https://fedorabg.bg.ac.rs/fedora/get/o:10881/bdef:Content/download
no
Micromorphological and cytological analysis of trichomes and biological effects of extracts of Salvia aegyptiaca L., S. fruticosa Mill. and S. lanigera Poir. (Lamiaceae) from Libya
Mikromorfološka i citološka analiza trihoma i biološki efekti ekstrakata Salvia aegyptiaca L., S. fruticosa Mill. i S. lanigera Poir. (Lamiaceae) iz Libije
[ "Duletić-Laušević, Sonja" ]
[ "Marin, Petar", "Janošević, Dušica", "Džamić, Ana", "Budimir, Snežana" ]
Al Sheef, Najat Beleed
2016-04-10T11:23:01
[ "2016-04-10T11:23:01", "2020-07-03T08:07:31" ]
2015-12-25
[ "https://nardus.mpn.gov.rs/handle/123456789/5089", "http://eteze.bg.ac.rs/application/showtheses?thesesId=2739", "https://fedorabg.bg.ac.rs/fedora/get/o:10881/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025026482" ]
[ "" ]
[ "Three Libyan Salvia species were subjected to comprehensive study of microscopic characteristics of trichomes and biological activities of their extracts, while the selected species have not been studied previously. The micromorphology, ultrastructure and histochemistry of S. aegyptiaca, S. fruticosa and S. lanigera leaf trichomes were investigated using light, scanning and transmission electron microscopy. The leaves bear abundant unbranched non-glandular and two types of glandular trichomes - peltate and capitate, on both leaf surfaces. Since Salvia species are used for medicinal and cosmetic purposes, due to essential oils secreted in the glandular trichomes, the focus was on the analyses of the glandular trichomes. In S. aegyptiaca leaves the peltate trichomes were composed of basal cell, short cylindrical stalk cell and broad head of eight to twelve secretory cells arranged in a single circle, or more in two circles. Capitate trichomes (hairs) consisted of one-celled glandular head, subtended by a stalk of variable length, and classified into two types: capitate type I or short-stalked capitate and capitate type II or long-stalked capitate trichomes. Histochemical tests showed that the secreted material in all types of S. aegyptiaca glandular trichomes was of a complex nature. Positive reactions to lipids for all types of glandular trichomes were obtained, with especially abundant secretion observed in peltate and capitate trichomes type II. In S. fruticosa peltate hairs consisted of basal epidermal cell, very short stalk cell and large round head of eight secretory cells arranged in a circle. Capitate trichomes can be divided into two main types, short stalked and long stalked, and further into five subtypes according to the number of stalk cells, the morphology and the number of the glandular head cells. Digitiform trichomes consisted of one basal cell, one or two stalk cells and one apical secretory cell, which are of similar diameter and approximately equal length..." ]
application/pdf
en
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173029/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc/4.0/" ]
Универзитет у Београду
[ "Salvia aegyptiaca", "Salvia fruticosa", "Salvia lanigera", "micromorphology", "antioxidant activity", "antimicrobial activity", "cytotoxic activity", "neurodegenerative enzymes" ]
[ "Salvia aegyptiaca", "Salvia fruticosa", "Salvia lanigera", "mikromorfologija", "antioksidativna aktivnost", "antimikrobna aktivnost", "citotoksična aktivnost", "neurodegenerativni enzimi" ]
[ "Micromorphological and cytological analysis of trichomes and biological effects of extracts of Salvia aegyptiaca L., S. fruticosa Mill. and S. lanigera Poir. (Lamiaceae) from Libya", "Mikromorfološka i citološka analiza trihoma i biološki efekti ekstrakata Salvia aegyptiaca L., S. fruticosa Mill. i S. lanigera Poir. (Lamiaceae) iz Libije" ]
[ "" ]
doctoralThesis
[ "BY-NC" ]
Дулетић-Лаушевић, Соња; Марин, Петар; Јаношевић, Душица; Джамић, Aна; Будимир, Снежана; Aл Схееф, Најат Белеед;
[ "http://nardus.mpn.gov.rs/bitstream/id/1624/Disertacija746.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1625/Najat_Beleed_Al_Sheef_Referat_BF.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1624/Disertacija746.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1625/Najat_Beleed_Al_Sheef_Referat_BF.pdf", "https://fedorabg.bg.ac.rs/fedora/get/o:10881/bdef:Content/download" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_5089" ]
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https://nardus.mpn.gov.rs/handle/123456789/7572
123456789-7572.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:14512/bdef:Content/download
no
Развој атлетике на параолимпијским играма под утицајем нових технологија
Development of athletics at the paralympic under the influence of new technologies
[ "Stefanović, Đorđe" ]
[ "Mijatović, Slađana", "Juhas, Irina", "Mihajlović, Ilona" ]
Plakona, Eleni K.
2017-02-20T10:24:13
[ "2017-02-20T10:24:13", "2020-07-03T09:45:51" ]
2016-06-02
[ "https://nardus.mpn.gov.rs/handle/123456789/7572", "http://eteze.bg.ac.rs/application/showtheses?thesesId=4502", "https://fedorabg.bg.ac.rs/fedora/get/o:14512/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=514774700" ]
[ "Научно-технолошка револуција базирана на убрзаним друштвеним променама је допринела популаризацији и развоју Параолимпијског покрета, односно, врхунског спорта особа са инвалидитетом. Тежња ка остваривању све бољих резултата у врхунском спорту има за потребу да се улажу велики материјални и нематеријални ресурси. Напредак технологије и преданост спортиста постигли су то да некада недостижни резултати данас буду стварност. На Параолимпијским играма, као и на Олимпијским играма, од свих спортова, атлетске дисциплине привлаче највећи број спортиста и гледалаца. На атлетским такмичењима учествују и мушкарци и жене у свим категоријама инвалидитета. Проблем који се односи на развој атлетике на Параолимпијским играма под утицајем нових технологија није био предмет научних истраживања до сада. Највећи број истраживања у области спортских активности особа са инвалидитетом се односи на тренажни део у складу са степеном инвалидности. Питања која се односе на утицај нових технологија на развој атлетике на Параолимпијским играма захтевају одговарајуће научне одговоре. Чињеница је да је визија развоја Параолимпијског покрета у будућности у директној спрези са развојем нове технологије. У циљу постигнућа што бољих резултата ових спортиста, поред квалитетног тренажног процеса, отворена је могућност за побољшање квалитета њихових помагала. Квалитет помагала којима се служе спортисти са инвалидитетом се развијао и усавршавао паралелно са самим Параолимпијским покретом. Захваљујући провајдерима протетичких и ортопедских помагала и техничкој сервисној подршци, ови спортисти могу да се усредсреде на само такмичење. Предмет истраживања се односи на феномен атлетике која постоји у програму Параолимпијских игара – на настанак и развој атлетике под утицајем нових технологија..." ]
[ "" ]
application/pdf
sr
Универзитет у Београду, Факултет спорта и физичког васпитања
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]
Универзитет у Београду
[ "Параолимпијске игре", "атлетика", "резултат", "ортопедска помагала", "нове технологије" ]
[ "Paralympic Games", "track and field", "orthopedic aids", "new technologies" ]
[ "Развој атлетике на параолимпијским играма под утицајем нових технологија" ]
[ "Development of athletics at the paralympic under the influence of new technologies" ]
doctoralThesis
[ "BY-NC-ND" ]
Стефановић, Ђорђе; Мијатовић, Слађана; Јухас, Ирина; Михајловић, Илона; Плакона, Елени К.; Razvoj atletike na paraolimpijskim igrama pod uticajem novih tehnologija;
[ "https://nardus.mpn.gov.rs/bitstream/id/23760/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/23761/IzvestajKomisije7656.pdf", "http://nardus.mpn.gov.rs/bitstream/id/23760/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/23761/IzvestajKomisije7656.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_7572" ]
[ "" ]
[ "" ]
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https://nardus.mpn.gov.rs/handle/123456789/2038
123456789-2038.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:2135/bdef:Content/download
no
Biologija kozice Parapenaeus longirostris (Lucas, 1846) (Crustacea, Decapoda) na šelfu crnogorskog primorja
Biology of the deep water pink shrimp Parapenaeus longirostris (Lucas, 1846) (Crustacea, Decapoda) on the Montenegrin shelf
[ "Simonović, Predrag" ]
[ "Nikolić, Vera", "Petrov, Brigita", "Regner, Slobodan", "Joksimović, Aleksandar" ]
Marković, Olivera B.
2016-01-05T11:45:32
[ "2016-01-05T11:45:32", "2020-07-03T08:08:37" ]
2012-06-11
[ "https://nardus.mpn.gov.rs/handle/123456789/2038", "http://eteze.bg.ac.rs/application/showtheses?thesesId=16", "https://fedorabg.bg.ac.rs/fedora/get/o:2135/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=" ]
[ "Jedna od najvažnijih komercijalnih vrsta dekapodnih rakova u kočarskom ribolovu na šelfu Crnogorskog primorja je kozica, Parapenaeus longirostris (Lucas, 1846). Istraživanje je obavljeno tokom dvije godine, od novembra 2006. do oktobra 2007. i od juna 2009. do maja 2010. godine pri čemu su uzorci uzimani sa kočarskih brodova mjesečnom dinamikom. Tokom prve godine je ukupno obrađeno 1979 jedinki, od kojih su 1421 (71.80%) bile ženke a 558 (28.20%) mužjaci. Odnos polova je i u drugoj godini bio u korist ženki tokom koje je analizirano 1967 jedinki, od kojih su 1304 (66.30%) bile ženke a 663 (33.70%) mužjaci. Raspon dužina karapaksa svih ulovljenih jedinki zajedno je bio manji u prvoj godini i kretao se od 15 do 37 mm (25.23 ± 4.48) dok je u drugoj taj raspon bio 11-37 mm (24.85 ± 4.48). Uočen je polni dimorfizam: ženke su u prosjeku i duže i teže od mužjaka, i u dužinskim grupama >28- 30 mm CL su 100% prisutne u ulovu. Mužjaci su u boljoj kondiciji od ženki. Vrijednosti alometrijskog koeficijenata b dužinsko-težinskog odnosa u prvoj godini iznose b = 2.58 za ukupan uzorak, b = 2.51 za ženke i b = 2.34 za mužjake; dok te vrijednosti za drugu godinu iznose b = 2.59 za ukupan uzorak, b = 2.57 za ženke i b = 2.33 za mužjake. Prva polna zrelost ženki je nastupila pri dužini karapaksa od 20.48 mm u prvoj godini, odnosno od 19.89 mm CL u drugoj godini..." ]
[ "Parapenaeus longirostris (Lucas, 1846) is one of the most economically important decapod species in the trawl fishery on the Montenegrin shelf (southern Adriatic Sea). Despite its importance, there were no biological studies on the species for that area. Data were obtained from monthly samples taken from commercial catches realized in the southern Adriatic during the period from November 2006 to October 2007 and from June 2009 to May 2010. During the first year among the 1979 specimens, 71.80% were females and 28.20% males, while during the second year of investigation, among the 1967 specimens, 66.30% were females and 33.70% males. The overall sex ratio was in favour of females. The carapace length of all specimens ranged from 15 to 37 mm, with a mean of 25.23 ± 4.48, while during the second year the range of carapace length of all specimens was wider and ranged from 11 to 37 mm, with a mean of 24.85 ± 4.48. A marked sexual dimorphism was observed between males and females: females have a wider size range, greater size and weight. Percentage of females reached 100% in the 28-30 mm size class and beyond. Males were in better condition than females. The calculated allometric coefficient of length-weight relationships was b= 2.58 for the total sample of 2006-2007 year, b= 2.51 for females and b= 2.34 for males; while during the 2009-2010 values were b=2.59 for the total sample, b=2.57 for females and b=2.33 for males. The size at which 50% of the female population reached maturity was at a carapace length of 20.48 mm in the first year and 19.89 mm during the second year..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]
Универзитет у Београду
[ "Parapenaeus longirostris", "biologija", "kočarski ribolov", "šelf", "prelov", "Crnogorsko primorje", "Južni Jadran" ]
[ "Parapenaeus longirostris", "biology", "demersal trawl fishery", "shelf", "overexploited stock", "Montenegrin coast", "South Adriatic" ]
[ "Biologija kozice Parapenaeus longirostris (Lucas, 1846) (Crustacea, Decapoda) na šelfu crnogorskog primorja", "Biology of the deep water pink shrimp Parapenaeus longirostris (Lucas, 1846) (Crustacea, Decapoda) on the Montenegrin shelf" ]
[ "" ]
doctoralThesis
[ "BY-NC-SA" ]
Симоновић, Предраг; Јоксимовић, Aлександар; Регнер, Слободан; Петров, Бригита; Николић, Вера; Марковић, Оливера Б.; Биологија козице Парапенаеус лонгирострис (Луцас, 1846) (Црустацеа, Децапода) на шелфу црногорског приморја; Биологија козице Парапенаеус лонгирострис (Луцас, 1846) (Црустацеа, Децапода) на шелфу црногорског приморја;
[ "https://nardus.mpn.gov.rs/bitstream/id/1921/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1921/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_2038" ]
10.2298/bg20120611markovic
[ "" ]
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[ "" ]
https://nardus.mpn.gov.rs/handle/123456789/11534
123456789-11534.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:20301/bdef:Content/download
no
Asocijacija genskih varijanti na hromozomu 9p21 i transkripcije gena za CDKN2B i HACD4 sa nastankom ateroskleroze i njenim kliničkim komplikacijama kod čoveka
Association of genetic variants in chromoseome 9p21 and transcription of CDKN2B and HACD4 genes with the onset of atherosclerosis and its clinical complications in human.
[ "Živković, Maja" ]
[ "Brajušković, Goran", "Đurić, Tamara", "Živković, Maja", "Brajušković, Goran" ]
Životić, Ivan
2019-11-15T12:36:56
[ "2019-11-15T12:36:56", "2020-07-03T08:07:56" ]
2019-05-14
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6931", "https://nardus.mpn.gov.rs/handle/123456789/11534", "https://fedorabg.bg.ac.rs/fedora/get/o:20301/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025217714" ]
[ "Ateroskleroza je kompleksna, hronična i progresivna bolest arterijskih krvnih sudova koja se razvija tokom života i najčešće se klinički manifestuje u kasnijem životnom dobu. Dve najznačajnije kliničke komplikacije su ishemijski moždni i srčani udar (IM), na čiji nastanak utiču sredinski i nasledni faktori (genetska predispozicija). U ovoj studiji ispitana je asocijacija četiri genetičke varijante prisutne u hromozomskom regionu 9p21 (rs10757278 i rs518394 u genu ANRIL, i rs36212560 i rs2275888 u genu HACD4), sa rizikom za nastanak IM, karotidnog plaka (KP) i kliničkih komplikacija KP (ultrasonografski definisan tip plaka, simptomatska karotidna bolest, prisustvo ulceracije na plaku). Ispitana je transkripcija gena CDKN2B i HACD4 kao i nivo iRNK u tkivu karotidnog aterosklerotskog plaka i mononuklearnim ćelijama periferne krvi (MĆPK) bolesnika koji su doživeli prvi IM, 6 meseci nakon infarkta. Nivo iRNK ispitan je u odnosu na genotipove i tip karotidnog plaka. Utvrđena je značajna asocijacija rs10757278 sa rizikom za nastanak KP kod žena. Utvrđeno je da je haplotip genetičkih varijanti rs36212560 i rs2275888 značajno asociran sa rizikom za nastanak KP i IM. Rezutlat je pokazao polno specifičnu asocijaciju (kod muškaraca) kao i značajnu asocijaciju varijante rs36212560 testirane van haplotipa. Nije detektovana značajna asocijacija genetičkih varijanti rs2275888 i rs518394 sa rizikom za razvoj karotidnog plaka, ali je ustanovljena značajna, nezavisna asocijacija varijante rs2275888 sa rizikom za nastanak IM. Varijanta rs10757278 je značajno asocirana sa tipom karotidnog aterosklerotskog plaka. Ustanovljeno je da je haplotip varijanti rs36212560 i rs2275888 značajno asociran sa rizikom za razvoj ulceracije karotidnog plaka, i sa simptomatskom karotidnom bolešću u grupi bolesnika sa KP, kao i da polno specifična asocijacija sa haplotipom postoji kod muškaraca. Razlika u nivoima ekspresije merena je na nivou iRNK u odnosu na genotipove ispitivanih varijanti. U tkivu KP nije detektovana razlika u nivou iRNK za CDKN2B u odnosu na genotipove ispitivanih varijanti, ali je ustanovljen statistički trend u asocijaciji sa genotipom varijante rs1075278. Varijanta rs2275888 je prethodno okarakterisan kao eQTL (eng. expression Quantitative Trait Locus) za gen FOCAD gen u tibijalnoj arteriji..." ]
[ "Atherosclerosis is a complex, chronic and progeressive disease of arterial blood vessels that develops during the life and usualy with clinical manifestations at later age. Two most singnifficant clinical manifestations are stroke and myocardial infarction (MI), which development is affected by the median and hereditary factors (genetic predisposition). In this study, association of four genetic variants from chromosome region 9p21 have been investigated (rs10757278 and rs518394 in the ANRIL gene, and rs36212560 and rs2275888 in the HACD4 gene) with the risk for development of MI, the carotid plaque (CP) and its clinical complications (ultrasonographicaly deffined plaque type, symphtomatic disease, presence of carotid plaque ulceration). The transcription of genes CDKN2B and HACD4 at the mRNA level was examined in the CP tissue and peripheral blood mononuclear cells (PBMC) of patients who sustained first MI, six months after the infarction. Level of mRNA was examined in relation to genotypes and CP type. Signifficant association was determined for rs10757278 with the risk for CP development among females. It was determined that haplotype of gene variants rs36212560 and rs2275888 is significantly associated with the risk for CP and MI development. Result has shown gender specific association (among males) as well as association of rs36212560 with CP and MI when tested independently. It wasn’t detected signifficant association of gene variants rs2275888 and rs518394 with the risk for the development of CP, but, the signifficant idependant association of rs2275888 variant with the risk for MI. Variant rs10757278 is signifficantly associated with the carotid atherosclerotic plaque type. Haplotype of rs36212560 and rs2275888 variants is signifficantly associated with the carotid plaque ulceration, as well as with simpthomatic carotid disease in the group of CP patients., and in addition, the gender speciffic association with the haplotype exist in males. Diference in expression levels was measured at the level of mRNA in relation to genotypes of tested variants. In CP tissue difference in CDKN2B mRNK level wasn’t detected in relation to tested variants according to genotype, but statistical trend was detected in association with rs10757278 genotypes..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41028/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by/4.0/" ]
Универзитет у Београду
[ "studija asocijacije", "genetičke varijante", "karotidni plak", "infarkt miokarda", "9p21", "CDKN2B", "HACD4", "FOCAD", "iRNK", "relativna ekspresija" ]
[ "association study", "genetic variants", "carotid plaque (CP)", "myocardial infarction (MI)", "9p21", "CDKN2B", "HACD4", "FOCAD", "mRNK" ]
[ "Asocijacija genskih varijanti na hromozomu 9p21 i transkripcije gena za CDKN2B i HACD4 sa nastankom ateroskleroze i njenim kliničkim komplikacijama kod čoveka" ]
[ "Association of genetic variants in chromoseome 9p21 and transcription of CDKN2B and HACD4 genes with the onset of atherosclerosis and its clinical complications in human." ]
doctoralThesis
[ "BY" ]
[ "http://nardus.mpn.gov.rs/bitstream/id/1740/IzvestajKomisije21142.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1739/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1740/IzvestajKomisije21142.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1739/Disertacija.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_11534" ]
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https://nardus.mpn.gov.rs/handle/123456789/9980
123456789-9980.pdf
yes
no
https://fedorabg.bg.ac.rs/fedora/get/o:18344/bdef:Content/download
no
Efekat estradiola na regulaciju endotelne i inducibilne azot-monoksid-sintaze u srcu gojaznih pacova
Effect of estradiol on regulation of endothelial and inducible nitric oxide synthase in the heart of obese rats
[ "Isenović, Esma R." ]
[ "Đorđević, Jelena", "Sudar Milovanović, Emina", "Jasnić, Nebojša", "Labudović-Borović, Milica" ]
Zafirović, Sonja S.
2018-10-09T09:44:26
[ "2018-10-09T09:44:26", "2020-07-03T08:07:18" ]
2018-05-09
[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=6031", "https://nardus.mpn.gov.rs/handle/123456789/9980", "https://fedorabg.bg.ac.rs/fedora/get/o:18344/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50433295" ]
[ "Estradiol ostvaruje pozitivan efekat na kardiovaskularni sistem (KVS), sprečavanjem nastanka ateroskleroze i endotelne i/ili vaskularne disfunkcije, a deluje i direktno na srce smanjujući njegovu hipertrofiju. Estradiol ostvaruje pozitivne efekte u KVS regulacijom azot-monoksid (NO)-sintaza (NOS), endotelne (eNOS) i inducibilne (iNOS) forme, koje su odgovorne za sintezu NO, aktivacijom sloţene mreţe unutarćelijskih signalnih puteva, u koje su uključeni signalni molekuli supstrat receptora za insulin 1 (IRS-1), fosfatidil-inozitol-3-kinaza (PI3K) i protein kinaza B (Akt). Estradiol utiče na signalnu transdukciju angiotenzina II (Ang II), delujući na ekspresiju receptora za Ang II tipa 1 (AT1R) i tipa 2 (AT2R), kao i na nivo RhoA proteina. Estradiol utiče i na transport energetskih supstrata regulišući translokazu masnih kiselina (CD36) i transportere za glukozu tipa 1 (GLUT1) i tipa 4 (GLUT4). Kontrolni i gojazni muţjaci pacova soja Wistar tretirani su estradiolom (40μg/kg) ili 1% etanolom 24 sata pre ţrtvovanja. Spektrofotometrijskim metodama određivane su koncentracije NO u plazmi i koncentracije L-Arginina (L-Arg), NO i slobodnih masnih kiselina (SMK) u lizatu srca. Western blot metodom određivani su nivoi proteina eNOS, iNOS, NF-κB-p65, pIRS-1/IRS-1, p85 i p110 subjedinice PI3K, pAkt/Akt, RhoA, AT1R, AT2R, GLUT1, GLUT4 i CD36 proteina, kao i asocijacija IRS-1 sa p85-PI3K proteinom u srcu pacova, dok je imunohistohemijskom metodom određivana ekspresija i lokalizacija eNOS i iNOS. Metodom qRT-PCR određivan je nivo iRNK za eNOS i iNOS u srcu pacova. Rezultati su analizirani korišćenjem Studentovog t-testa. Rezultati prikazani u ovoj doktorskoj disertaciji pokazuju da tretman estradiolom kod kontrolnih pacova povećava nivo p85 subjedinice PI3K i fosforilaciju Akt na Thr308, dok smanjuje ekspresiju gena za iNOS..." ]
[ "Estradiol has a positive effect on the cardiovascular system (CVS) by preventing the development of atherosclerosis and endothelial and/or vascular dysfunction. Estradiol exerts many of its effects on the CVS by regulation of nitric oxide synthase (NOS), endothelial (eNOS) and inducible (iNOS) forms that are responsible for the synthesis of nitric oxide (NO), through activation of the complex network of intercellular signal pathways that include signaling molecules: insulin receptor substrate 1 (IRS-1), phosphatidylinositol 3-kinase (PI3K) and protein kinase B (Akt.) Estradiol also affects the signal transduction of angiotensin II (Ang II) by affecting the expression of Ang II receptors: type 1 (AT1R) and type 2 (AT2R), as well as the level of RhoA protein. Finally, estradiol indirectly regulates the transport of energy substrates in heart, by regulating transporters of free fatty acids (FFA) and glucose, CD36 and glucose transporters type 1 (GLUT1) and type 4 (GLUT4). Control and obese male Wistar rats were treated with one dose of estradiol (40 μg/kg) or with the same amount of 1% ethanol in saline 24 hours before sacrificed. The concentration of NO was measured in the plasma and in lysates, while the concentrations of L-Arginine and FFA were determined in the lysates of the rats‘ hearts. The Western blot method was used to determine the level of eNOS, iNOS, NF-kB p65, IRS-1, p85 and p110 subunits of PI3K, Akt, AT1R, AT2R, RhoA, CD36, GLUT1 and GLUT4 in the rats‘ heart, as well as association of IRS-1 with p85 subunit of PI3K. Immunohistochemical analysis was used for localization and expression of eNOS and iNOS proteins in heart. qRT-PCR was used to determen the level of iRNA of eNOS and iNOS in rats‘ heart. Results were analyzed using Student‘s t test..." ]
application/pdf
sr
Универзитет у Београду, Биолошки факултет
[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173033/RS//" ]
[ "openAccess" ]
[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]
Универзитет у Београду
[ "estradiol", "srce", "eNOS", "iNOS", "energetski supstrati" ]
[ "estradiol", "heart", "eNOS", "iNOS", "metabolic substrates" ]
[ "Efekat estradiola na regulaciju endotelne i inducibilne azot-monoksid-sintaze u srcu gojaznih pacova" ]
[ "Effect of estradiol on regulation of endothelial and inducible nitric oxide synthase in the heart of obese rats" ]
doctoralThesis
[ "BY-NC-SA" ]
Исеновић, Есма Р.; Ђорђевић, Јелена; Лабудовић-Боровић, Милица; Јаснић, Небојша; Судар Миловановић, Емина; Зафировић, Соња С.; Ефекат естрадиола на регулацију ендотелне и индуцибилне азот-моноксид-синтазе у срцу гојазних пацова; Ефекат естрадиола на регулацију ендотелне и индуцибилне азот-моноксид-синтазе у срцу гојазних пацова;
[ "http://nardus.mpn.gov.rs/bitstream/id/1562/IzvestajKomisije17726.pdf", "http://nardus.mpn.gov.rs/bitstream/id/1561/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1561/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/1562/IzvestajKomisije17726.pdf" ]
[ "https://hdl.handle.net/21.15107/rcub_nardus_9980" ]
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