text: Virtual CROI 2020 Highlights of Epidemiology Public Health and Prevention Research At the 2020 Conference on Retroviruses and Opportunistic Infections held virtually as a result of the emerging COVID 19 pandemic trends in the HIV epidemic were highlighted with decreasing HIV incidence reported across several countries although key regions remain heavily impacted including the US South Adolescent girls and young women men who have sex with men MSM transgender persons and people who inject drugs continue to experience a high burden of new infections Sexually transmitted infections during pregnancy can lead to a number of adverse outcomes in infants novel strategies to detect and treat these infections are needed Innovative HIV testing strategies including self testing and assisted partner services are expanding the reach of testing however linkage to care can be improved Novel preexposure prophylaxis PrEP delivery strategies are increasing uptake of PrEP in different groups although adherence and persistence remain a challenge Use of on demand PrEP is increasing among MSM in the US Strategies are needed to address barriers to PrEP uptake and persistence among cis and transgender women Several novel regimens for postexposure prophylaxis show promise 