text: Exploring the Regulatory Function of the i N i terminal Domain of SARS CoV 2 Spike Protein through Molecular Dynamics Simulation SARS CoV 2 is what has caused the COVID 19 pandemic Early viral infection is mediated by the SARS CoV 2 homo trimeric Spike S protein with its receptor binding domains RBDs in the receptor accessible state Molecular dynamics simulation on the S protein with a focus on the function of its i N i terminal domains NTDs is performed The study reveals that the NTD acts as a wedge and plays a crucial regulatory role in the conformational changes of the S protein The complete RBD structural transition is allowed only when the neighboring NTD that typically prohibits the RBDs movements as a wedge detaches and swings away Based on this NTD wedge model it is proposed that the NTD RBD interface should be a potential drug target 