text:  Application of the PCR number of cycle threshold value Ct in COVID 19 The SARS CoV 2 pandemic persists with all its virulence despite 650 382 819 doses of COVID vaccine worldwide The reference test for infection identification is reverse transcription polymerase chain reaction RT qPCR The usefulness of this test may be diminished by simplifying its result as positive or negative Determining the number of cycles Ct in positive RT qPCR tests can assist in decision making when interpreted in the clinical context of patients 